Métodoestesis: Los caminos del sentir en los saberes de la tierra una aventura geo-epistémica en clave sur

The word Metodoestesis (The paths of feeling), invented by Ana Patricia Noguera, names the epistemic trajectories described in this article. The first is the Ecosystem - Culture proposal, prepared by Augusto Ángel- Maya, one of the creators of Latin American Environmental Thought. In it, Augusto deviated from the Sustainable Development model, to think of the environment as emerging from the intersections between natural capital, social capital and economic capital, proposing to think of the environment as emerging from the relationships between ecosystems and cultures. The permanent contacts between these two systems opened the door to a systemic-aesthetic environmental thought. The second path is Body - Earth, emerging from the work of Ana Patricia Noguera. In this proposal, the path of feeling is placed in the bodies-between-bodies, which in their intertactality are transforming and dissolving each other. This aesthetic episteme opens the way to the ethical, as what is configured in these intertactalities and not as a meta-theory of values. From the two previous trajectories arises the episteme of the knowledge of the earth. In it, the organization of knowledge emerges from the earth in an ecopoietic key. From the Methodology, as a weaving of paths of feeling, we are interested in asking ourselves how we are inhabiting the earth.La palabra Métodoestesis (Los caminos del sentir) creada por Ana Patricia Noguera, nombra las trayectorias epistémicas que se describen en este artículo. La primera es la propuesta Ecosistema – Cultura, elaborada por Augusto Ángel-Maya, uno de los creadores del Pensamiento Ambiental Latinoamericano. En ella, Augusto de apartó del modelo de Desarrollo Sostenible, para pensar lo ambiental como lo emergente de las intersecciones entre el capital natural, el capital social y el capital económico, proponiendo pensar lo ambiental como lo emergente de las relaciones entre los ecosistemas y las culturas. Los contactos permanentes entre estos dos sistemas, abrieron la puerta a un pensamiento ambiental sistémicoestético. La segunda trayectoria es Cuerpo – Tierra, emergente de la obra de Ana Patricia Noguera. En esta propuesta, el camino del sentir se coloca en los cuerpos-entre-cuerpos, que en su intertactalidad van transformándose y disolviéndose unos en otros. Esta episteme estética, abre camino a lo ético, como lo que se configura en dichas intertactalidades y no como una metateoría de valores. De las dos trayectorias anteriores surge la episteme de los saberes de la tierra. En ella, la organización de los saberes emerge de la tierra en clave ecopoiésica. Desde la Metodoestesis, como tejido de caminos del sentir nos interesa preguntarnos por cómo estamos habitando la tierra

Hepatitis B: Inmunogenicidad de la vacuna recombinante cubana anti-hbv en trabajadores de la salud vacunados sin seroprotección

We measured ant-HBs to 153 health care workers in the Children Hospital Lorencita Villegas de Santos in Bogota who had receive Hepatitis B vaccine in the last 4 years. 124 had receive three doses (6 with booster), 18 two doses and 11 only one. They hadn't any positive titles in 25%, 33% and 73% respectively. Their average age was 40 years old. Then we gave 32 of the worker who has negative titles one 20 mgms dose of the Cuban recombinant vaccine and we measured blind titles before and 15 days after with ELISA quantitative method. 81% went into seroconversion (> O UYL), 75% seroprotection (> 10 IUIL) and 56% had levels over 100 UVL. This results are hilly significant and raises a possibility for health care workers who are "poor antibody responders" with other types of vaccines or even other population with the same problem.Medimos anti-HBVs a 153 trabajadores de la salud del Hospital Infantil Universitario, "Lorencita Villegas de Santos" en Santafé de Bogotá, a quien se les había vacunado contra la Hepatitis B en los últimos 4 años. 124 habían recibido tres dosis (6 con refuerzo), 18 dos dosis y 11 una sola dosis; no tenían títulos el 25%. 33% Y 73% respectivamente. Estos trabajadores tenían promedios de edad de 40 años. Se vacunaron 32 de estos trabajadores sin títulos con 1 dosis de 20 mgms de la vacuna cubana recombinante y se cuantificaron títulos anti-HBVs antes y 15 días después de la vacunación con método inmuno-enzimático cuantitativo a ciegas; obteniéndose seroconversión en el 81% (> UI/L), seroprotección en el 75% (> 10 UI/L), y 56% de los individuos tuvieron valores superiores a 100 UI/L. Estos resultados son altamente significativos y dan una alternativa para un grupo de trabajadores de la salud que no ha logrado títulos protectores y plantea un posibilidad para las poblaciones con factores que los cataloguen como "malos respondedores"

Combinatorial Use of Chitosan Nanoparticles, Reversine, and Ionising Radiation on Breast Cancer Cells Associated with Mitosis Deregulation

Breast cancer is the most commonly occurring cancer in women worldwide and the second most common cancer overall. The development of new therapies to treat this devastating malignancy is needed urgently. Nanoparticles are one class of nanomaterial with multiple applications in medicine, ranging from their use as drug delivery systems and the promotion of changes in cell morphology to the control of gene transcription. Nanoparticles made of the natural polymer chitosan are easy to produce, have a very low immunogenic profile, and diffuse easily into cells. One hallmark feature of cancer, including breast tumours, is the genome instability caused by defects in the spindle-assembly checkpoint (SAC), the molecular signalling mechanism that ensures the timely and high-fidelity transmission of the genetic material to an offspring. In recent years, the use of nanoparticles to treat cancer cells has gained momentum. This is in part because nanoparticles made of different materials can sensitise cancer cells to chemotherapy and radiotherapy. These advances prompted us to study the potential sensitising effect of chitosan-based nanoparticles on breast cancer cells treated with reversine, which is a small molecule inhibitor of Mps1 and Aurora B that induces premature exit from mitosis, aneuploidy, and cell death, before and after exposure of the cancer cells to X-ray irradiation. Our measurements of metabolic activity as an indicator of cell viability, DNA damage by alkaline comet assay, and immunofluorescence using anti-P-H3 as a mitotic biomarker indicate that chitosan nanoparticles elicit cellular responses that affect mitosis and cell viability and can sensitise breast cancer cells to X-ray radiation (2Gy). We also show that such a sensitisation effect is not caused by direct damage to the DNA by the nanoparticles. Taken together, our data indicates that chitosan nanoparticles have potential application for the treatment of breast cancer as adjunct to radiotherapy

Ética Profesional y Responsabilidad Social Universitaria

este libro compila reflexiones y experiencias en responsabilidad social y ética profesional desde instituciones de Educación Superior. La responsabilidad social universitaria, como ámbito de investigación y de desarrollo conceptual y metodológico es transversal a las universidades, tanto desde el punto de vista organizacional, como desde el misional e investigativo. Quienes impulsen la responsabilidad social, requieren de ética profesional, que debe ser la clave para la construcción de principios que guíen a empresarios, políticos, gestores sociales, investigadores, entre otros, para lograr consensuar el a veces difícil equilibrio entre el bien común y el desarrollo personal

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Métodoestesis: Los caminos del sentir en los saberes de la tierra una aventura geo-epistémica en clave sur

The word Metodoestesis (The paths of feeling), invented by Ana Patricia Noguera, names the epistemic trajectories described in this article. The first is the Ecosystem - Culture proposal, prepared by Augusto Ángel- Maya, one of the creators of Latin American Environmental Thought. In it, Augusto deviated from the Sustainable Development model, to think of the environment as emerging from the intersections between natural capital, social capital and economic capital, proposing to think of the environment as emerging from the relationships between ecosystems and cultures. The permanent contacts between these two systems opened the door to a systemic-aesthetic environmental thought. The second path is Body - Earth, emerging from the work of Ana Patricia Noguera. In this proposal, the path of feeling is placed in the bodies-between-bodies, which in their intertactality are transforming and dissolving each other. This aesthetic episteme opens the way to the ethical, as what is configured in these intertactalities and not as a meta-theory of values. From the two previous trajectories arises the episteme of the knowledge of the earth. In it, the organization of knowledge emerges from the earth in an ecopoietic key. From the Methodology, as a weaving of paths of feeling, we are interested in asking ourselves how we are inhabiting the earth.La palabra Métodoestesis (Los caminos del sentir) creada por Ana Patricia Noguera, nombra las trayectorias epistémicas que se describen en este artículo. La primera es la propuesta Ecosistema – Cultura, elaborada por Augusto Ángel-Maya, uno de los creadores del Pensamiento Ambiental Latinoamericano. En ella, Augusto de apartó del modelo de Desarrollo Sostenible, para pensar lo ambiental como lo emergente de las intersecciones entre el capital natural, el capital social y el capital económico, proponiendo pensar lo ambiental como lo emergente de las relaciones entre los ecosistemas y las culturas. Los contactos permanentes entre estos dos sistemas, abrieron la puerta a un pensamiento ambiental sistémicoestético. La segunda trayectoria es Cuerpo – Tierra, emergente de la obra de Ana Patricia Noguera. En esta propuesta, el camino del sentir se coloca en los cuerpos-entre-cuerpos, que en su intertactalidad van transformándose y disolviéndose unos en otros. Esta episteme estética, abre camino a lo ético, como lo que se configura en dichas intertactalidades y no como una metateoría de valores. De las dos trayectorias anteriores surge la episteme de los saberes de la tierra. En ella, la organización de los saberes emerge de la tierra en clave ecopoiésica. Desde la Metodoestesis, como tejido de caminos del sentir nos interesa preguntarnos por cómo estamos habitando la tierra

La carrera de Enseñanza de la Matemática: pasado y futuro en la Sede de Occidente de la Universidad de Costa Rica

En este artículo se abordan los detalles más importantes que favorecieron el fortalecimiento de la carrera de Enseñanza de la Matemática en la Sede de Occidente de la Universidad de Costa Rica (UCR), tomando en consideración los dos recintos de esta sede. Se indican cuáles han sido los cursos ofrecidos, los profesores que los han impartido, la cantidad de graduados por semestre y se menciona el impacto de la carrera en las instituciones de educación secundaria de la región de Occidente. Se describe la organización interna de la carrera, el enlace que se establece con la Escuela de Matemática, la Escuela de Formación Docente de la Sede Rodrigo Facio y con el Departamento de Educación de la Sede de Occidente. Se analizan las principales fortalezas y debilidades que tiene la carrera en la actualidad según la percepción de los autores y de la opinión de veinticinco estudiantes de cuarto y quinto año, esta última obtenida por medio de una encuesta. Por último se presentan algunas ideas generales que permitan iniciar una discusión para el planteamiento de una carrera propia. In this article the most important details which favored the strength of the Mathematics Major at Sede de Occidente of the Universidad de Costa Rica (UCR) are board, taking into consideration the two campuses of the Sede. Courses offered, professors who taughtthose courses, and the number of graduated students by semester is indicated. Also, the major´s impact, over the Secondary Schools from the Occidental Region, is mentioned. The internal organization of the major is described, and its established link with the Mathematics School, the Education School from Sede Rodrigo Facio and the Education Department from Sede de Occidente. The main strengths and weaknesses of the current major are analyzed according to the authors´ perception and the opinion of twenty-five students from the fourth and fifth years, data obtained by means of a questionnaire. Finally, some general ideas to initiate a discussion for the planning of a proper major to belong to the Sede de Occidente are presented

Cultivo in vitro de Anaplasma marginale en líneas celulares endoteliales

Anaplasma marginale, intracellular bacteria causes bovine anaplasmosis, characterized by progressive anemia, fever, decrease in body weight and death occasionally. The use of A. marginale in vitro cell culture - derived has been suggested as an alternative for development new prevention tools and control strategies.Anaplasma marginale, bacteria intracelular obligada, es el agente causal de la anaplasmosis bovina, caracterizada por anemia progresiva, fiebre, pérdida de peso y ocasionalmente, la muerte. Como alternativa para el desarrollo de nuevas estrategias de prevención se plantea el uso de cultivos celulares para la obtención in vitro de A. marginale

Modification of Proliferation and Apoptosis in Breast Cancer Cells by Exposure of Antioxidant Nanoparticles Due to Modulation of the Cellular Redox State Induced by Doxorubicin Exposure

In this report, we investigated whether the use of chitosan-carrying-glutathione nanoparticles (CH-GSH NPs) can modify proliferation and apoptosis, and reduce cell damage induced by doxorubicin on breast cancer cells. Doxorubicin is a widely used antineoplasic agent for the treatment of various types of cancer. However, it is also a highly toxic drug because it induces oxidative stress. Thus, the use of antioxidant molecules has been considered to reduce the toxicity of doxorubicin. CH-GSH NPs were characterized in size, zeta potential, concentration, and shape. When breast cancer cells were treated with CH-GSH nanoparticles, they were localized in the cellular cytoplasm. Combined doxorubicin exposure with nanoparticles increased intracellular GSH levels. At the same time, decreasing levels of reactive oxygen species and malondialdehyde were observed and modified antioxidant enzyme activity. Levels of the Ki67 protein were evaluated as a marker of cell proliferation and the activity of the Casp-3 protein related to cell apoptosis was measured. Our data suggests that CH-GSH NPs can modify cell proliferation by decreasing Ki67 levels, induce apoptosis by increasing caspase-3 activity, and reduce the oxidative stress induced by doxorubicin in breast cancer cells by modulating molecules associated with the cellular redox state. CH-GSH NPs could be used to reduce the toxic effects of this antineoplastic. Considering these results, CH-GSH NPs represent a novel delivery system offering new opportunities in pharmacy, material science, and biomedicine