Effects of aqueous extract of Moringa oleifera seed on cadmium-induced reproductive toxicity in male Wistar rats

Bacground: Moringa oleifera seeds have been reported to increase sexual activity of male rats but its mechanism of action remains unknown.Objective: Cadmium is a heavy metal that induces reproductive toxicity. To elucidate its possible mechanisms of action, the effects of aqueous extract of Moringa seeds (AEMS) on Cadmium-induced reproductive damage were investigated.Methods: Thirty male rats (180-200g, n=5) were grouped as follows: Control, Cadmium (2mg/kg), AEMS (100 and 500mg/kg), Cadmium+100mg/kg AEMS and Cadmium+500mg/kg AEMS. Single intraperitoneal dose of Cadmium was administered while AEMS was given daily (p.o) for 6 weeks and thereafter sacrificed by cervical dislocation. Sperm variables were examined microscopically while serum was analysed for sex hormones and antioxidants, testicular and epididymal total protein from tissue homogenate. Data were analysed using Students t-test and ANOVA.Results: Cadmium caused significant decrease in sperm variables. The AEMS decreased sperm motility, count and testosterone. These declines were dose dependent. Total testicular protein was significantly lowered by AEMS but total epididymal protein was not affected. Serum catalase and SOD was reduced by AEMS while MDA increased.Conclusion: Moringa seeds may exhibit male reproductive toxicity, as observed from its deleterious effect on androgen and sperm variables. It failed to prevent cadmium-induced alteration in reproductive variables and serum antioxidants.Keywords: Aqueous extract, Moringa oleifera seed, cadmium-induced reproductive toxicity, male Wistar rats

Effects of aqueous extract of Moringa oleifera seed on cadmium-induced reproductive toxicity in male Wistar rats

Bacground: Moringa oleifera seeds have been reported to increase sexual activity of male rats but its mechanism of action remains unknown. Objective: Cadmium is a heavy metal that induces reproductive toxicity. To elucidate its possible mechanisms of action, the effects of aqueous extract of Moringa seeds (AEMS) on Cadmium-induced reproductive damage were investigated. Methods: Thirty male rats (180-200g, n=5) were grouped as follows: Control, Cadmium (2mg/kg), AEMS (100 and 500mg/ kg), Cadmium+100mg/kg AEMS and Cadmium+500mg/kg AEMS. Single intraperitoneal dose of Cadmium was administered while AEMS was given daily (p.o) for 6 weeks and thereafter sacrificed by cervical dislocation. Sperm variables were examined microscopically while serum was analysed for sex hormones and antioxidants, testicular and epididymal total protein from tissue homogenate. Data were analysed using Students t-test and ANOVA. Results: Cadmium caused significant decrease in sperm variables. The AEMS decreased sperm motility, count and testosterone. These declines were dose dependent. Total testicular protein was significantly lowered by AEMS but total epididymal protein was not affected. Serum catalase and SOD was reduced by AEMS while MDA increased. Conclusion: Moringa seeds may exhibit male reproductive toxicity, as observed from its deleterious effect on androgen and sperm variables. It failed to prevent cadmium-induced alteration in reproductive variables and serum antioxidants

Thermal diffusivity measurement for p-Si and Ag/p-Si by photoacoustic technique

Thermal diffusivity (TD) of p-Si and Ag/p-Si samples were measured by photoacoustic technique using open photoacoustic cell (OPC). The samples were annealed by heating them at 960, 1050, 1200, and 1300 °C for 3 h in air. The thermal diffusivity of Ag-coated samples was obtained by fitting the photoacoustic experimental data to the thermally thick equation for Rosencwaig and Gersho (RG) theory. For the single layer samples, the thermal diffusivity can be obtained by fitting as well as by obtaining the critical frequency f c . In this study, the thermal diffusivity of the p-Si samples increased with increasing the annealing temperature. The thermal diffusivity of the Ag/p-Si samples, after reaching the maximum value of about 2.73 cm2/s at a temperature of 1200 °C, decreased due to the silver complete melt in the surface of the silicon

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background: Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods: This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was coprioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low-middle-income countries. Results: In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of 'single-use' consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low-middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion: This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high- and low-middle-income countries