Support for Same-Sex Marriage at Record High, but Key Segments Remain Opposed

As the Supreme Court prepares to decide a key case involving states' requirements to recognize same-sex marriage, public support for allowing gays and lesbians to marry legally continues its rapid rise: A 57%-majority of Americans now favor allowing same-sex marriage and 39% oppose. As recently as five years ago, more opposed (48%) same-sex marriage than supported it (42%).This is the highest level of support measured for same-sex marriage in nearly 20 years of Pew Research Center polling of the issue. Yet even as support for same-sex marriage has increased among nearly all segments in the public, some groups remain broadly opposed to gay marriage.The Pew Research Center survey, conducted May 12-18 among 2,002 adults, finds that partisans are as divided on this issue as ever: Today, 65% of Democrats and an identical percentage of independents favor gay marriage; only about one third (34%) of Republicans do so. Growing shares of all three groups support same-sex-marriage, yet the differences between Democrats and Republicans are as wide today as they were a decade ago

A Deep Dive Into Party Affiliation: Sharp Differences by Race, Gender, Generation, Education

Democrats hold advantages in party identification among blacks, Asians, Hispanics, well-educated adults and Millennials. Republicans have leads among whites -- particularly white men, those with less education and evangelical Protestants -- as well as members of the Silent Generation.A new analysis of long-term trends in party affiliation among the public provides a detailed portrait of where the parties stand among various groups in the population. It draws on more than 25,000 interviews conducted by the Pew Research Center in 2014, which allows examination of partisan affiliation across even relatively small racial, ethnic, educational and income subgroups

Speaking rate and articulation rate of native speakers of Irish English

Previous studies have shown apparent difference in speaking rate and/or articulation rate between different dialects of English. In addition, native speakers of Irish English are perceived to be speaking very fast; however, there is very little information in the current literature regarding speech rate of speakers of this variety of English. Establishing regional norms might be needed for various reasons, such as speech intervention, if speakers of Irish English do have a higher speaking and articulation rates than individuals who speaks other English dialects. This study measured the speaking rate and articulation rate of 22 men and 22 women who were native speakers of Irish English. The study compared the speech rates between the male and female speakers, and across different speech tasks â first and second reading the Rainbow Passage and spontaneous speech elicited in a conversation. Overall, the male speakers seemed to speak faster than the females, although significant difference was found only in the articulation rate for the second reading of the passage. The speakers showed significantly higher speaking rate during passage reading than in conversation but the difference in articulation rate between the two tasks was not significant. Finally, the Irish English speakers in this study appeared to have relatively higher speech rates than most of the English dialects reported in previous studies

Slip length dependent propulsion speed of catalytic colloidal swimmers near walls

Catalytic colloidal swimmers that propel due to self-generated fluid flows exhibit strong affinity for surfaces. We here report experimental measurements of significantly different velocities of such microswimmers in the vicinity of substrates made from different materials. We find that velocities scale with the solution contact angle $\theta$ on the substrate, which in turn relates to the associated hydrodynamic substrate slip length, as $V\propto(\cos\theta+1)^{-3/2}$. We show that such dependence can be attributed to osmotic coupling between swimmers and substrate. Our work points out that hydrodynamic slip at the wall, though often unconsidered, can significantly impact the self-propulsion of catalytic swimmers

A novel live cell assay to measure diacylglycerol lipase α activity

Diacylglycerol lipase α (DAGLα) hydrolyses DAG to generate the principal endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) in the central nervous system. DAGLα dependent cannabinoid (CB) signalling has been implicated in numerous processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measurement of diacylglycerol lipase (DAGL) activity using its native substrate generally involves low-throughput MS techniques. Some relatively high-throughput membrane based assays utilizing surrogate substrates have been reported, but these do not take into account the rate-limiting effects often associated with the ability of a drug to cross the cell membrane. In the present study, we report the development of a live cell assay to measure DAGLα activity. Two previously reported DAGLα surrogate substrates, p-nitrophenyl butyrate (PNPB) and 6,8-difluoro-4-methylumbelliferyl octanoate (DiFMUO), were evaluated for their ability to detect DAGLα activity in live cell assays using a human cell line stably expressing the human DAGLα transgene. Following optimization, the small molecule chromogenic substrate PNPB proved to be superior by providing lower background activity along with a larger signal window between transfected and parental cells when compared with the fluorogenic substrate DiFMUO. The assay was further validated using established DAGL inhibitors. In summary, the live cell DAGLα assay reported here offers an economical and convenient format to screen for novel inhibitors as part of drug discovery programmes and compliments previously reported high-throughput membrane based DAGL assays

Phenotypic Plasticity in Uveal Melanoma Is Not Restricted to a Tumor Subpopulation and Is Unrelated to Cancer Stem Cell Characteristics

Purpose: Uveal melanoma (UM) is the most common primary intraocular malignancy in adults and approximately half of those diagnosed will die of metastasis. This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed “cancer stem cells” (CSCs). Methods: Expression of postulated stem cell markers aldehyde dehydrogenase (ALDH), CD44, and CD133 was analyzed in UM cell lines and primary UM short-term cultures (STCs) established from tumor samples. Additionally, the notion of a “cellular hierarchy” within UM was investigated. Finally, the phenomenon of phenotypic plasticity in response to environmental factors was explored. Results: We demonstrate that expression of ALDH, CD44, and CD133 does not select for a subpopulation of stem-like cells in either UM cell lines or UM STCs. Furthermore, there is an absence of a cellular hierarchy in cell lines and all cells in culture are able to drive tumor progression. Last, we show that established UM cell lines and UM STCs are plastic in nature and switch their phenotype in response to environmental stimuli. Conclusions: We hypothesize that this capacity to undergo phenotypic plasticity may be a consequence of neural crest lineage and renders the exploration of the CSC hypothesis extremely challenging in UM

Poly-ε-Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens

Microbial keratitis (MK) is a serious issue in many countries and is often caused by contact lens wear. Antimicrobial peptides (AMPs) are a potentially useful tool for creating antimicrobial surfaces in light of increasing antibiotic resistance. Poly-ε-lysine (pεK) is an AMP that has been used extensively as a food preservative and Mel4 has recently been synthesized and studied as an antimicrobial coating for contact lenses. A hydrogel synthesized of pεK cross-linked with biscarboxylic acids provides a potential lens material which has many surface free amines, that can be subsequently used to attach additional AMPs, creating an antimicrobial lens. The aim of this study is to investigate pεK hydrogels against a clinical strain of Pseudomonas aeruginosa (P. aeruginosa) for preventing or treating MK. Covalent attachment of AMPs is investigated and confirmed by fluorescently tagged peptides. Bound pεK effectively reduces the number of adherent P. aeruginosa in vitro (>3 log). In ex vivo studies positive antimicrobial activity is observed on bare pεK hydrogels and those with additionally bound pεK or Mel4; lenses allow the maintenance of the corneal epithelium. A pεK hydrogel contact lens with additional AMPs can be a therapeutic tool to reduce the incidence of MK

British Council School Programmes in Scotland: an Impact Study: Final Report

This report evaluates the impact of British Council programmes in Scottish schools, particularly their contribution to closing the attainment gap for students from different socioeconomic backgrounds. British Council Scotland works with approximately a third of all schools in Scotland through programmes, including Erasmus+, Connecting Classrooms, e-Twinning, and Modern Language Assistants, that enrich curriculum, pedagogy and professional development with a global outlook. Despite a variety of definitions and treatments, there is growing attention to global citizenship education and sustainability education as important curricular goals across nations and systems.The research reported herein sought to document the impact of BC programmes in Scottish schools, and its contribution to the Attainment Challenge’s focus on achieving both excellence and equity. It used a mixed methods approach of quantitative data analysis (using open national data and individual pupil data) in combination with qualitative case studies of differently positioned schools and interviews with senior staff in key stakeholder agencies