4,565 research outputs found
Methods to Isolate Possible Bacteriophage for Micrococcus Luteus and Acinetobacter Baumannii
The increasing prevalence of antibiotic-resistant strains of bacteria has led to a crisis in treatment options. Acinetobacter baumannii is an example of a bacterium that has developed a dangerous level of multidrug resistance. Not only does it have genes allowing for the resistance to antibiotics, but it also produces a biofilm that protects it. In recent years, A. baumannii has become a major contributor to nosocomial infections making it critical to develop new treatment methods. Micrococcus luteus, while typically not thought of as a pathogen, is also developing a resistance to antibiotics. M. luteus is capable of forming a biofilm on its own making it worrisome as it has increasingly been noted as an opportunistic pathogen.
One potential new treatment of antibiotic resistance is the development of bacteriophage therapy, using bacterial viruses to target the infection and treat it. This study examines methods for isolating novel bacteriophage from dairy cattle feces, specifically for the biofilm producers A. baumannii and M. luteus
Immediate replacement of fishing with dairying by the earliest farmers of the NE Atlantic archipelagos
The appearance of farming, from its inception in the Near East around 12 000 years ago, finally reached the northwestern extremes of Europe by the fourth millennium BC or shortly thereafter. Various models have been invoked to explain the Neolithization of northern Europe; however, resolving these different scenarios has proved problematic due to poor faunal preservation and the lack of specificity achievable for commonly applied proxies. Here, we present new multi-proxy evidence, which qualitatively and quantitatively maps subsistence change in the northeast Atlantic archipelagos from the Late Mesolithic into the Neolithic and beyond. A model involving significant retention of hunter–gatherer–fisher influences was tested against one of the dominant adoptions of farming using a novel suite of lipid biomarkers, including dihydroxy fatty acids, ω-(o-alkylphenyl)alkanoic acids and stable carbon isotope signatures of individual fatty acids preserved in cooking vessels. These new findings, together with archaeozoological and human skeletal collagen bulk stable carbon isotope proxies, unequivocally confirm rejection of marine resources by early farmers coinciding with the adoption of intensive dairy farming. This pattern of Neolithization contrasts markedly to that occurring contemporaneously in the Baltic, suggesting that geographically distinct ecological and cultural influences dictated the evolution of subsistence practices at this critical phase of European prehistory
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Accessing and assessing lunar resources with PROSPECT
PROSPECT is a package in development by ESA to assess the in-situ resource potential of lunar regolith. PROSPECT will: obtain sub-surface regolith samples, extract volatiles, identify chemical species, quantify abundances, and characterize isotopes
Do ultrafast exciton-polaron decoherence dynamics govern photocarrier generation efficiencies in polymer solar cells?
All-organic-based photovoltaic solar cells have attracted considerable
attention because of their low-cost processing and short energy payback time.
In such systems the primary dissociation of an optical excitation into a pair
of photocarriers has been recently shown to be extremely rapid and efficient,
but the physical reason for this remains unclear. Here, two-dimensional
photocurrent excitation spectroscopy, a novel non-linear optical spectroscopy,
is used to probe the ultrafast coherent decay of photoexcitations into
charge-producing states in a polymer:fullerene based solar cell. The
two-dimensional photocurrent spectra are interpreted by introducing a
theoretical model for the description of the coupling of the electronic states
of the system to an external environment and to the applied laser fields. The
experimental data show no cross-peaks in the two-dimensional photocurrent
spectra, as predicted by the model for coherence times between the exciton and
the photocurrent producing states of 20\,fs or less
Ion Trap Mass Spectrometers for Identity, Abundance and Behavior of Volatiles on the Moon
NASA GSFC and The Open University (UK) are collaborating to deploy an Ion Trap Mass Spectrometer on the Moon to investigate the lunar water cycle. The ITMS is flight-proven throughthe Rosetta Philae comet lander mission. It is also being developed under ESA funding to analyse samples drilled from beneath the lunar surface on the Roscosmos Luna-27 lander (2025).Now, GSFC and OU will now develop a compact ITMS instrument to study the near-surface lunar exosphere on board a CLPS Astrobotic lander at Lacus Mortis in 2021
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Investigating TriHaloMethanes with respect to humidity
The disinfection of potable water has dramatically reduced the instances of Cholera and similar ailments within a population drawing upon that water source. There is however the possibility that Natural Organic Matter (NOM) can interact with the disinfection compounds to form Disinfection By-Products (DBP). One group of DBPs are Trihalomethanes (THMs) with several compounds of the group being suspected carcinogens. Within the UK the total concentration of all THMs within drinking water must not exceed 100µg/l.
At present water authorities take samples of the water supply and return them to a central laboratory for analysis. This provides an accurate test but one which can involve a long lead time in discovering a potential hazard to public health.
A Field Asymmetric Ion Mobility Spectrometer (FAIMS) sensor may be ideally placed to perform in situ continuous monitoring at particular sites. As part of a PhD co-sponsored by The Open University and Owlstone Nanotech Plc an investigation is ongoing to discover how sensitive a FAIMS device is with respect to THMs and humidity when sampling. Initial results and the method of data processing, which involves peak fitting to evolving spectra are presented
Exceptional Heterogeneity in Viral Evolutionary Dynamics Characterises Chronic Hepatitis C Virus Infection.
The treatment of HCV infection has seen significant progress, particularly since the approval of new direct-acting antiviral drugs. However these clinical achievements have been made despite an incomplete understanding of HCV replication and within-host evolution, especially compared with HIV-1. Here, we undertake a comprehensive analysis of HCV within-host evolution during chronic infection by investigating over 4000 viral sequences sampled longitudinally from 15 HCV-infected patients. We compare our HCV results to those from a well-studied HIV-1 cohort, revealing key differences in the evolutionary behaviour of these two chronic-infecting pathogens. Notably, we find an exceptional level of heterogeneity in the molecular evolution of HCV, both within and among infected individuals. Furthermore, these patterns are associated with the long-term maintenance of viral lineages within patients, which fluctuate in relative frequency in peripheral blood. Together, our findings demonstrate that HCV replication behavior is complex and likely comprises multiple viral subpopulations with distinct evolutionary dynamics. The presence of a structured viral population can explain apparent paradoxes in chronic HCV infection, such as rapid fluctuations in viral diversity and the reappearance of viral strains years after their initial detection.status: publishe
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MoonLITE – Technological feasibility of the penetrator concept
Introduction: While the surface missions to the Moon of the 1960s and 1970s achieved a great deal, scientifically a great deal was also left unresolved. The recent plethora of lunar missions (flown or proposed) reflects resurgence in interest in the Moon, not only in its own right, but also as a record of the formation of the Earth-Moon System and the interplanetary environment at 1 AU. Results from orbiter missions have indicated the possible presense of ice within permanently shaded craters at the lunar poles [1] – a situation that, if confirmed, will have profound impacts on lunar exploration
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