Direct Observation of Self-Assembled Chain-Like Water Structures in a Nanoscopic Water Meniscus

Sawtooth-like oscillatory forces generated by water molecules confined between two oxidized silicon surfaces were observed using a cantilever-based optical interfacial force microscope when the two surfaces approached each other in ambient environments. The humidity-dependent oscillatory amplitude and periodicity were 3-12 nN and 3-4 water diameters, respectively. Half of each period was matched with a freely jointed chain model, possibly suggesting that the confined water behaved like a bundle of water chains. The analysis also indicated that water molecules self-assembled to form chain-like structures in a nanoscopic meniscus between two hydrophilic surfaces in air. From the friction force data measured simultaneously, the viscosity of the chain-like water was estimated to be between 108 and 1010 times greater than that of bulk water. The suggested chain-like structure resolves many unexplained properties of confined water at the nanometer scale, thus dramatically improving the understanding of a variety of water systems in nature

The kinematic footprints of five stellar streams in Andromeda's halo

(abridged) We present a spectroscopic analysis of five stellar streams (`A', `B', `Cr', `Cp' and `D') as well as the extended star cluster, EC4, which lies within streamC, all discovered in the halo of M31 from our CFHT/MegaCam survey. These spectroscopic results were initially serendipitous, making use of our existing observations from the DEep Imaging Multi-Object Spectrograph mounted on the Keck II telescope, and thereby emphasizing the ubiquity of tidal streams that account for ~70% of the M31 halo stars in the targeted fields. Subsequent spectroscopy was then procured in streamCr/p and streamD to trace the velocity gradient along the streams. For the cluster EC4, candidate member stars with average [Fe/H]~-1.4 (Fe/H_spec=-1.6), are found at v_{hel}=-285 km/s suggesting it could be related to streamCp. No similarly obvious cold kinematic candidate is found for streamD, although candidates are proposed in both of two spectroscopic pointings along the stream (both at -400 km/s). Spectroscopy near the edge of streamB suggests a likely kinematic detection, while a candidate kinematic detection of streamA is found (plausibly associated to M33 rather than M31). The low dispersion of the streams in kinematics, physical thickness, and metallicity makes it hard to reconcile with a scenario whereby these stream structures as an ensemble are related to the giant southern stream. We conclude that the M31 stellar halo is largely made up of multiple kinematically cold streams.Comment: 19 pages, 12 figures, accepted in MNRAS. High resolution version, with fig10 here: http://www.ast.cam.ac.uk/~schapman/streams.pd

Dynamics in the satellite system of Triangulum: Is AndXXII a dwarf satellite of M33?

We present results from a spectroscopic survey of the dwarf spheroidal And XXII and the two extended clusters EC1 and EC2. These three objects are candidate satellites of the Triangulum galaxy, M33, which itself is likely a satellite of M31. We use the DEep Imaging Multi-Object Spectrograph mounted on the Keck-II telescope to derive radial velocities for candidate member stars of these objects and thereby identify the stars that are most likely actual members. Eleven most probable stellar members (of 13 candidates) are found for AndXXII. We obtain an upper limit of sigma_v < 6.0 km s-1 for the velocity dispersion of AndXXII, [Fe/H] ~ -1.6 for its metallicity, and 255pc for the Plummer radius of its projected density profile. We construct a colour magnitude diagram for AndXXII and identify both the red giant branch and the horizontal branch. The position of the latter is used to derive a heliocentric distance to And XXII of 853 pm 26 kpc. The combination of the radial velocity, distance, and angular position of AndXXII indicates that it is a strong candidate for being the first known satellite of M33 and one of the very few examples of a galactic satellite of a satellite. N-body simulations imply that this conclusion is unchanged even if M31 and M33 had a strong encounter in the past few Gyr. We test the hypothesis that the extended clusters highlight tidally stripped galaxies by searching for an excess cloud of halo-like stars in their vicinity. We find such a cloud for the case of EC1 but not EC2. The three objects imply a dynamical mass for M33 that is consistent with previous estimates.Comment: 14 pages, 14 figures, revised for MNRAS publicatio

Analysis of subcellular metabolite levels of potato tubers (Solanum tuberosum) displaying alterations in cellular or extracellular sucrose metabolism

The expression of a heterologous invertase in potato tubers (Solanum tuberosum) in either the cytosol or apoplast leads to a decrease in total sucrose content and to an increase in glucose. Depending on the targeting of the enzyme different changes in phenotype and metabolism of the tubers occur: the cytosolic invertase expressing tubers show an increase in the glycolytic flux, accumulation of amino acids and organic acids, and the appearance of novel disaccharides; however, these changes are not observed when the enzyme is expressed in the apoplast [Roessner et al. (2001). Plant Cell, 13, 11-29]. The analysis of these lines raised several questions concerning the regulation of compartmentation of metabolites in potato tubers. In the current study we addressed these questions by performing comparative subcellular metabolite profiling. We demonstrate that: (i) hexoses accumulate in the vacuole independently of their site of production, but that the cytosolic invertase expression led to a strong increase in the cytosolic glucose concentration and decrease in cytosolic sucrose, whereas these effects were more moderate in the apoplastic expressors; (ii) three out of four of the novel compounds found in the cytosolic overexpressors accumulate in the same compartment; (iii) despite changes in absolute cellular content the subcellular distribution of amino acids was invariant in the invertase overexpressing tubers. These results are discussed in the context of current models of the compartmentation of primary metabolism in heterotrophic plant tissues

Global mortality and readmission rates following COPD exacerbation-related hospitalisation: a meta-analysis of 65 945 individual patients

\ua9 2024, European Respiratory Society. All rights reserved.Background Exacerbations of COPD (ECOPD) have a major impact on patients and healthcare systems across the world. Precise estimates of the global burden of ECOPD on mortality and hospital readmission are needed to inform policy makers and aid preventive strategies to mitigate this burden. The aims of the present study were to explore global in-hospital mortality, post-discharge mortality and hospital readmission rates after ECOPD-related hospitalisation using an individual patient data meta-analysis (IPDMA) design. Methods A systematic review was performed identifying studies that reported in-hospital mortality, postdischarge mortality and hospital readmission rates following ECOPD-related hospitalisation. Data analyses were conducted using a one-stage random-effects meta-analysis model. This study was conducted and reported in accordance with the PRISMA-IPD statement. Results Data of 65 945 individual patients with COPD were analysed. The pooled in-hospital mortality rate was 6.2%, pooled 30-, 90- and 365-day post-discharge mortality rates were 1.8%, 5.5% and 10.9%, respectively, and pooled 30-, 90- and 365-day hospital readmission rates were 7.1%, 12.6% and 32.1%, respectively, with noticeable variability between studies and countries. Strongest predictors of mortality and hospital readmission included noninvasive mechanical ventilation and a history of two or more ECOPD-related hospitalisation

Sucrose to starch: a transition in molecular plant physiology [Review]

The major flux in potato tuber carbon metabolism is the conversion of sucrose through hexose phosphates to starch. The enzymes that mediate this pathway are well characterized, the genes that encode them have been cloned and transgenic plants have been generated. These transgenic studies have confirmed hypotheses based on more indirect methods, but they have also generated new challenges by highlighting the enormous flexibility and complexity inherent in plant metabolism. The investigation of the sucrose-to-starch transition in potato tubers is an excellent example of how the discipline of molecular plant physiology is evolving at both the scientific and technical levels. [References: 65] 6