Remodeling Office for Growing Grounds

This project was my first real re-condition project for a portion of an office space for Growing Grounds Farm here in San Luis Obispo. The purpose of this essay is to look back onto my experience and see how much I struggled and learned, and how I can benefit from it in the future. Throughout my project, I made sure there was constant communication between myself and Craig Wilson, program manager for Growing Grounds Farm, with clarifications and several updates. I made sure to fit and complete the project within my tight schedule and adjust it throughout the process. Throughout the project there were several building adaptations and tweaks I had to make in this unique space in order to install, and build out an office safely and efficiently. There were some new and difficult experiences for me that I had to learn for the first time as I was constructing the new office space. Throughout this process, I had a new appreciation and gained more sympathy for those who do this work on a daily basis

Vegetation Diversity in Natural and Restored Forested Wetland Sites in Southeast Arkansas

The loss of forested wetlands in the Lower Mississippi River Alluvial Valley in Arkansas has altered regional vegetation communities. Multiple restoration projects have been established in this region to restore wetlands and the services they provide. In order to return these functions to the environment, microtopographic features were constructed in 2001 at Bob White Memorial Wetlands Research and Teaching Station (Bob White). Vegetation diversity was examined at Cut-Off Creek Wildlife Management Area (Cutoff), a naturally forested wetland, and Bob White, an area formally converted to cropland that is now undergoing forest wetland restoration. Vegetation diversity is one way to determine if restoration efforts are effectively restoring ecosystem structure and functions to natural wetland conditions. Vegetation diversity and composition were examined across three topographical features: hummocks/ridges, swales, and flats. Vegetation diversity was examined in the spring, summer, and fall. Indices were used for determining composition similarities between Bob White and Cutoff. Bob White had a species richness of 33 and Cutoff’s species richness was 47. Beta diversity between the two sites was 76 species, this value is high and suggests there is low similarity between the two sites. Sorensen-Dice Similarity Index value was calculated as 0.05, where on a scale of zero to one a low value indicates low similarity in composition. The low similarity between the two sites suggests that vegetation composition at Bob White has not been fully restored to conditions comparable to a natural setting. An explanation for this is the presence of Baccharis halimifolia (Eastern baccharis). B. halimifolia inhibits other species from colonizing. Another factor for the difference between the vegetation at Bob White and Cutoff is that Cutoff is an older forest. Hydrophyte communities in a forested wetland take 50 years after restoration begins for them to resemble a natural forested wetland. The results from this study provide mixed evidence that restoration at Bob White is succeeding; there is a high percentage of wetlands species, while vegetation lacks similarity. This study improves our understanding of the influence that anthropogenic changes have on wetland functions as agricultural lands are restored to their previous land cover. Ecosystem functions should continue to be monitored to determine time frames as these functions are restored to Bob Whit

Ethnic Differences in Children’s Entry into Public Mental Health Care via Emergency Mental Health Services

For children and youth making a mental health crisis visit, we investigated ethnic disparities in whether the children and youth were currently in treatment or whether this crisis visit was an entry or reentry point into mental health treatment. We gathered Medicaid claims for mental health services provided to 20,110 public-sector clients ages 17 and younger and divided them into foster care and non-foster care subsamples. We then employed logistic regression to analyze our data with sociodemographic and clinical controls. Among children and youth who were not placed in foster care, African Americans, Latinos, and Asian Americans were significantly less likely than Caucasians to have received mental health care during the three months preceding a crisis visit. Disparities among children and youth in foster care were not statistically significant. Ethnic minority children and youth were more likely than Caucasians to use emergency care as an entry or reentry point into the mental health treatment, thereby exhibiting a crisis-oriented pattern of care

Soft X-ray seeding studies for the SLAC Linac Coherent Light Source II

We present the results from studies of soft X-ray seeding options for the LCLS-II X-ray free electron laser (FEL) at SLAC. The LCLS-II will use superconducting accelerator technology to produce X-ray pulses at up to 1 MHz repetition rate using 4 GeV electron beams. If properly seeded, these pulses will be nearly fully coherent, and highly stable in photon energy, bandwidth, and intensity, thus enabling unique experiments with intense high-resolution soft X-rays. Given the expected electron beam parameters from start to end simulations and predicted FEL performance, our studies reveal echo enabled harmonic generation (EEHG) and soft X-ray self-seeding (SXRSS) as promising and complementary seeding methods. We find that SXRSS has the advantage of simplicity and will deliver 5-35 times higher spectral brightness than EEHG in the 1-2 nm range, but lacks some of the potential for phase-stable multipulse and multicolor FEL operations enabled by external laser seeding with EEHG

Possible new Arkansas endemic plant revealed by DNA sequence analysis, A

Cardamine angustata var. ouachitana, a wildflower in the mustard family (Brassicaceae), was described by Smith in 1982 to include a form of Cardamine found only in the Ouachita Mountains of Arkansas. This variety is morphologically very similar to typical Cardamine angustata. The major difference noted by Smith for the two varieties was the complete lack of leaf hairs (trichomes) in the new variety, whereas typical Cardamine angustata normally possesses trichomes. However, Al-Shehbaz rejected the variety ouachitana and reduced it to synonymy with the typical C. angustata. The recommendation of Al-Shehbaz has been followed and the taxon Cardamine angustata var. ouachitana is currently not accepted by most plant taxonomists. We performed a preliminary evaluation of the status of Cardamine angustata var. ouachitana by comparing ribosomal internal transcribed spacer region DNA sequences from specimens of Cardamine angustata var. ouachitana with sequences of Cardamine angustata from the main range of the species and other related species of Cardamine. Phylogenetic analyses of these data produced an unexpected result; specimens of C. angustata var. ouachitana were actually closely related to C. concatenata, rather than the expected close relationship with C. angustata. However, C. angustata var. ouachitana is morphologically distinct from C. concatenata. These results suggest that Cardamine angustata var. ouachitana is actually a new species found only in the Ouachita Mountains of Arkansas

Aerial dissemination of Clostridium difficile spores

Background: Clostridium difficile-associated diarrhoea (CDAD) is a frequently occurring healthcare-associated infection, which is responsible for significant morbidity and mortality amongst elderly patients in healthcare facilities. Environmental contamination is known to play an important contributory role in the spread of CDAD and it is suspected that contamination might be occurring as a result of aerial dissemination of C. difficile spores. However previous studies have failed to isolate C. difficile from air in hospitals. In an attempt to clarify this issue we undertook a short controlled pilot study in an elderly care ward with the aim of culturing C. difficile from the air. Methods: In a survey undertaken during February (two days) 2006 and March (two days) 2007, air samples were collected using a portable cyclone sampler and surface samples collected using contact plates in a UK hospital. Sampling took place in a six bedded elderly care bay (Study) during February 2006 and in March 2007 both the study bay and a four bedded orthopaedic bay (Control). Particulate material from the air was collected in Ringer's solution, alcohol shocked and plated out in triplicate onto Brazier's CCEY agar without egg yolk, but supplemented with 5 mg/L of lysozyme. After incubation, the identity of isolates was confirmed by standard techniques. Ribotyping and REP-PCR fingerprinting were used to further characterise isolates. Results: On both days in February 2006, C. difficile was cultured from the air with 23 samples yielding the bacterium (mean counts 53 – 426 cfu/m3 of air). One representative isolate from each of these was characterized further. Of the 23 isolates, 22 were ribotype 001 and were indistinguishable on REP-PCR typing. C. difficile was not cultured from the air or surfaces of either hospital bay during the two days in March 2007. Conclusion: This pilot study produced clear evidence of sporadic aerial dissemination of spores of a clone of C. difficile, a finding which may help to explain why CDAD is so persistent within hospitals and difficult to eradicate. Although preliminary, the findings reinforce concerns that current C. difficile control measures may be inadequate and suggest that improved ward ventilation may help to reduce the spread of CDAD in healthcare facilities

Simulation Studies of the X-Ray Free-Electron Laser Oscillator

Simulations of the x-ray free-electron laser (FEL) oscillator are presented that include transverse effects and realistic Bragg crystal properties with the two-dimensional code GINGER. In the present cases considered the radiation divergence is much narrower than the crystal acceptance, and the numerical algorithm can be simplified by ignoring the finite angular bandwidth of the crystal. In this regime GINGER shows that the saturated x-ray pulses have 109 photons and are nearly Fourier-limited with peak powers in excess of 1 MW. Wealso include preliminary results for a four-mirror cavity that can be tuned in wavelength over a few percent, with future plans to incorporate the full transverse response of the Bragg crystals into GINGER to more accurately model this tunable source

Photosynthetic pigments of oceanic chlorophyta belonging to prasinophytes clade VII

Preprin