Stable populations in unstable habitats: temporal genetic structure of the introduced ascidian Styela plicata in North Carolina

14 páginas, 3 tablas, 4 figuras.The analysis of temporal genetic variability is an essential yet largely neglected tool to unveil and predict the dynamics of introduced species. We here describe the temporal genetic structure and diversity over time of an introduced population of the ascidian Styela plicata (Lesueur, 1823) in Wilmington (North Carolina, USA, 34°08′24″N, 77°51′44″W). This population suffers important salinity and temperature changes, and in June every year we observed massive die-offs, leaving free substratum that was recolonized within a month. We sampled 12–14 individuals of S. plicata every 2 months from 2007 to 2009 (N = 196) and analyzed a mitochondrial marker (the gene cytochrome oxidase subunit I, COI) and seven nuclear microsatellites. Population genetic analyses showed similar results for both types of markers and revealed that most of the genetic variation was found within time periods. However, analyses conducted with microsatellite loci also showed weak but significant differences among time periods. Specifically, in the samplings after die-off episodes (August–November 2007 and 2008) the genetic diversity increased, the inbreeding coefficient showed prominent drops, and there was a net gain of alleles in the microsatellite loci. Taken together, our results suggest that recruits arriving from neighboring populations quickly occupied the newly available space, bringing new alleles with them. However, other shifts in genetic diversity and allele loss and gain episodes were observed in December–January and February–March 2008, respectively, and were apparently independent of die-off events. Overall, our results indicate that the investigated population is stable over time and relies on a periodic arrival of larvae from other populations, maintaining high genetic diversity and a complex interplay of allele gains and losses.This research was supported by a grant from the United States–Israel Binational Science Foundation (BSF), Jerusalem, Israel (number 2014025), the Spanish Government project CTM2013—48163—and the Catalan Government Grant 2014SGR-336 for Consolidated Research Groups.Peer reviewe

Simulation of static and random errors on Grover's search algorithm implemented in a Ising nuclear spin chain quantum computer with few qubits

We consider Grover's search algorithm on a model quantum computer implemented on a chain of four or five nuclear spins with first and second neighbour Ising interactions. Noise is introduced into the system in terms of random fluctuations of the external fields. By averaging over many repetitions of the algorithm, the output state becomes effectively a mixed state. We study its overlap with the nominal output state of the algorithm, which is called fidelity. We find either an exponential or a Gaussian decay for the fidelity as a function of the strength of the noise, depending on the type of noise (static or random) and whether error supression is applied (the 2pi k-method) or not.Comment: 18 pages, 8 figures, extensive revision with new figure

An observational study of the 7 September 2005 Barcelona tornado outbreak

This paper presents an observational study of the tornado outbreak that took place on the 7 September 2005 in the Llobregat delta river, affecting a densely populated and urbanised area and the Barcelona International airport (NE Spain). The site survey confirmed at least five short-lived tornadoes. Four of them were weak (F0, F1) and the other one was significant (F2 on the Fujita scale). They started mostly as waterspouts and moved later inland causing extensive damage estimated in 9 million Euros, three injured people but fortunately no fatalities. Large scale forcing was provided by upper level diffluence and low level warm air advection. Satellite and weather radar images revealed the development of the cells that spawned the waterspouts along a mesoscale convergence line in a highly sheared and relatively low buoyant environment. Further analysis indicated characteristics that could be attributed indistinctively to non-supercell or to mini-supercell thunderstorms

An observational study of the 7 September 2005 Barcelona tornado outbreak

This paper presents an observational study of the tornado outbreak that took place on the 7 September 2005 in the Llobregat delta river, affecting a densely populated and urbanised area and the Barcelona International airport (NE Spain). The site survey confirmed at least five short-lived tornadoes. Four of them were weak (F0, F1) and the other one was significant (F2 on the Fujita scale). They started mostly as waterspouts and moved later inland causing extensive damage estimated in 9 million Euros, three injured people but fortunately no fatalities. Large scale forcing was provided by upper level diffluence and low level warm air advection. Satellite and weather radar images revealed the development of the cells that spawned the waterspouts along a mesoscale convergence line in a highly sheared and relatively low buoyant environment. Further analysis indicated characteristics that could be attributed indistinctively to non-supercell or to mini-supercell thunderstorms

Phosphorus restriction does not prevent the increase in fibroblast growth factor 23 elicited by high fat diet

This study was designed to evaluate the influence of phosphorus (P) restriction on the dele- terious effects of high fat diets on mineral metabolism. Twenty-four rats were allotted to 3 groups (n = 8 each) that were fed different diets for 7 months. Rats in group 1 were fed nor- mal fat-normal P (0.6%) diet (NF-NP), rats in group 2 were fed high fat- normal P diet (HF- NP) and rats in group 3 were fed high fat-low P (0.2%) diet (HF-LP). Blood, urine and tissues were collected at the end of the experiments. When compared with the control group (NF- NP), rats fed HF diets showed increases in body weight, and in plasma concentrations of tri- glycerides and leptin, and decreased plasma calcitriol concentrations. In rats fed HF-NP plasma fibroblast growth factor 23 (FGF23) was higher (279.6 ± 39.4 pg/ml vs 160.6 ± 25.0 pg/ml, p = 0.018) and renal klotho (ratio klotho/GAPDH) was lower (0.75 ± 0.06 vs 1.06 ± 0.08, p < 0.01) than in rats fed NF-NP. Phosphorus restriction did not normalize plasma FGF23 or renal klotho; in fact, rats fed HF-LP, that only ingested an average of 22.9 mg/day of P, had higher FGF23 (214.7 ± 32.4 pg/ml) concentratio ns than rats fed NF-NP (160.6 ± 25. 0 pg/ml), that ingested and average of 74.4 mg/day of P over a 7 month period. In conclusion, our results demonstrate that severe P restriction over a prolonged period of time (7 months) does not normalize the increase in circulating FGF23 induced by HF diets. These data indi- cate that the deleterious effects of high fat diet on the FGF23/klotho axis are not eliminated by reduced P intake

Nueva información para la avifauna del estado de Querétaro, México

We present new information about 24 species, including 12 noteworthy records for the avifauna of the Mexican state of Queretaro. These data were gathered during fieldwork performed between 1993 to 2007, mainly in the Eje Neovolcánico province. Five species are considered protected under Mexican law NOM-059-SEMARNAT-2001: Buteogallus anthracinus, Buteo albonotatus, Trogon collaris, Turdus infuscatus and Vermivora crissalis. The checklist of birds present in Queretaro is now at least 347 species.Presentamos información nueva de 24 especies, incluyendo 12 nuevos registros de aves para el estado de Querétaro, México. Estos datos fueron obtenidos en diversos estudios de campo de 1993 a 2007, principalmente en la provincia del Eje Neovolcánico. Cinco especies están bajo alguna categoría de riesgo según la NOM-059-SEMARNAT-2001: Buteogallus anthracinus, Buteo albonotatus, Trogon collaris, Turdus infuscatus y Vermivora crissalis. Con estas adiciones el listado de aves de Querétaro se incrementa al menos a 347 especie

Relationship Between Bird Diversity and Habitat along a Pine- Oak Successional Forest in Southern Mexico

Few studies have related bird species richness and abundance with vegetation structure at different successional stages in pine-oak forests of Mexico. We studied changes in the bird community across three successional stages of pine-oak forest: early, intermediate, and mature. Also, we related bird community attributes—including generalist and specialist birds—with vegetation variables. We analyzed the vegetation and estimated bird abundances in 10-min-count periods in 10 circular plots per successional stage. We recorded a total of 71 bird species: 21 were specialists and 50 were generalists. The completeness of species richness was between 79 and 88% in the sampled habitats. Diversity profiles were consistently larger in the intermediate stage, except for its species richness that was not different between this and the early stage. We found a more richness and a higher number of individuals of generalist in the early and intermediate stages. The abundance of specialists was higher in the mature forest. An ordination analysis showed that generalists were associated to different variables. This suggests that these species can adapt to different forest conditions. Some specialist birds were more abundant in sites with high dominance of trees. Our results confirm the importance of maintaining not only mature forests but also young successional stages in order to conserve the species typical of secondary pine-oak forest bird species

Oral Acid Load Down-Regulates Fibroblast Growth Factor 23

Increased dietary acid load has a negative impact on health, particularly when renal function is compromised. Fibroblast growth factor 23 (FGF23) is a bone-derived hormone that is elevated during renal failure. The relationship between metabolic acidosis and FGF23 remains unclear. To investigate the effect of dietary acid load on circulating levels of FGF23, rats with normal renal function and with a graded reduction in renal mass (1/2 Nx and 5/6 Nx) received oral NH4Cl for 1 month. Acid intake resulted in a consistent decrease of plasma FGF23 concentrations in all study groups when compared with their non-acidotic control: 239.3 ± 13.5 vs. 295.0 ± 15.8 pg/mL (intact), 346.4 ± 19.7 vs. 522.6 ± 29.3 pg/mL (1/2 Nx) and 988.0 ± 125.5 vs. 2549.4 ± 469.7 pg/mL (5/6 Nx). Acidosis also decreased plasma PTH in all groups, 96.5 ± 22.3 vs. 107.3 ± 19.1 pg/mL, 113.1 ± 17.3 vs. 185.8 ± 22.2 pg/mL and 504.9 ± 75.7 vs. 1255.4 ± 181.1 pg/mL. FGF23 showed a strong positive correlation with PTH (r = 0.877, p < 0.0001) and further studies demonstrated that acidosis did not influence plasma FGF23 concentrations in parathyroidectomized rats, 190.0 ± 31.6 vs. 215 ± 25.6 pg/mL. In conclusion, plasma concentrations of FGF23 are consistently decreased in rats with metabolic acidosis secondary to increased acid intake, both in animals with intact renal function and with decreased renal function. The in vivo effect of metabolic acidosis on FGF23 appears to be related to the simultaneous decrease in PTH

Dinámica de uso y cobertura del suelo en un parque nacional mexicano

To understand the dynamics of land cover at the Parque Nacional Cofre de Perote, the rates of change in land use were compared at two different scales during the period 1995-2004. At the meso scale, these patterns were studied throughout the entire Parque Nacional Cofre de Perote, which is one of the 60 priority mountains of Mexico, and an important natural protected area of the country located in the state of Veracruz. At a micro scale, the work was focused in ejido El Conejo, located within the boundaries of this national park. Federal government digital orthophotos were used to determine changes in nine categories of land use. In both, the meso- and micro-scale, it was found that the predominant land cover categories are agriculture and forest. The probabilities of land cover change at both scales are low and essentially the same for most land use categories, reflecting both small gains in forest cover park-wide as well as the effectiveness of the ejido in managing natural resources within the park. The authors consider that the findings of the study may be applicable to the broader situation of national parks in Mexico and, finally, the importance of integrating local stakeholders in the management of natural protected areas is discussedPara entender la dinámica de la cobertura del suelo en el Parque Nacional Cofre de Perote, se hizo una comparación de las tasas de cambio de uso a dos escalas durante el periodo 1995-2004. A escala meso, se estudiaron estos patrones en toda la superficie del Parque Nacional Cofre de Perote, que es una de las 60 montañas prioritarias de México y también una importante área natural protegida del país ubicada en el estado de Veracruz. A escala micro el estudio se enfocó en el ejido El Conejo que se encuentra dentro de los límites de este parque nacional. Se utilizaron ortofotos digitales del gobierno federal para determinar el cambio en nueve categorías de uso del suelo. En ambas, meso y microescalas, se encontró que las categorías predominantes de cobertura son la agricultura y el bosque. Las probabilidades de cambio de cobertura del suelo en ambas escalas son bajas y tienen valores esencialmente iguales para la mayoría de las categorías de uso, reflejando tanto pequeñas ganancias en cobertura forestal en todo el parque como la efectividad del ejido en el manejo de recursos naturales dentro del parque. Se considera que los hallazgos de este estudio pueden ser aplicables a la situación prevaleciente en los restantes parques nacionales de México; al final se discute la importancia de integrar a los actores locales en el manejo de áreas naturales protegidasS

Energy-dense diets increase FGF23, lead to phosphorus retention and promote vascular calcifications in rats

Rats with normal renal function (Experiment 1, n = 12) and uninephrectomized (1/2Nx) rats (Experiment 2, n = 12) were fed diets with normal P (NP) and either normal (NF) or high fat (HF). Rats with intact renal function (Experiment 3, n = 12) were also fed NF or HF diets with high P (HP). Additionally, uremic (5/6Nx) rats (n = 16) were fed HP diets with NF or HF. Feeding the HF diets resulted in significant elevation of plasma FGF23 vs rats fed NF diets: Experiment 1, 593 ± 126 vs 157 ± 28 pg/ ml (p < 0.01); Experiment 2, 538 ± 105 vs 250 ± 18 pg/ml (p < 0.05); Experiment 3, 971 ± 118 vs 534 ± 40 pg/ml (p < 0.01). Rats fed HF diets showed P retention and decreased renal klotho (ratio klotho/actin) vs rats fed NF diets: Experiment 1, 0.75 ± 0.06 vs 0.97 ± 0.02 (p < 0.01); Experiment 2, 0.69 ± 0.07 vs 1.12 ± 0.08 (p < 0.01); Experiment 3, 0.57 ± 0.19 vs 1.16 ± 0.15 (p < 0.05). Uremic rats fed HF diet showed more severe vascular calcification (VC) than rats fed NF diet (aortic Ca = 6.3 ± 1.4 vs 1.4 ± 0.1 mg/g tissue, p < 0.001). In conclusion, energy-rich diets increased plasma levels of FGF23, a known risk factor of cardiovascular morbidity and mortality. Even though FGF23 has major phosphaturic actions, feeding HF diets resulted in P retention, likely secondary to decreased renal klotho, and aggravated uremic V