Un modelo de potencialidad climática para la trufa negra (Tuber melanosporum) en Teruel (España)

Black truffle (Tuber melanosporum) is an edible ectomycorrhizal mushroom whose cultivation is an economically-viable alternative in rural areas affected by depopulation. In the province of Teruel (Spain), truffle plantations occupy nowadays more than 4,500 ha, but no land use planning guidelines have been available. Species distribution models are tools that allow managers, farmers and foresters to plan ahead and make decisions with a scientific basis. The territory climatically suitable for T. melanosporum in Teruel has been predicted using a method based on the ecology field theory, previously tested in the development of distribution models for forest species. A dataset of 924 locations where black truffle has been collected in the Catalano-Aragonesa ecoregion and eight climatic parameters deeply influencing the presence and fructification of the black truffle have been considered and their corresponding habitats have been built. The distribution model, corrected under lithological criteria, has identified more than 8,000 km² as suitable for black truffle fructification in Teruel, almost 55% of the province, belonging 3,000 of them to the optimal class. This land is mainly distributed over the regions of Gúdar-Javalambre, Comunidad de Teruel and Maestrazgo. A further correction based on land-use criteria has identified more than 4,000 km² as suitable for truffle plantations, being 1,150 of them optimal. The model here presented will help to a proper planning for truffle cultivation in the province of Teruel.La trufa negra (Tuber melanosporum) es un hongo ectomicorrícico comestible cuyo cultivo supone en la actualidad una alternativa económica viable para zonas rurales afectadas gravemente por la despoblación. En la provincia de Teruel (España) hay más de 4.500 ha de plantaciones truferas, instaladas sin ningún proceso de planificación orientado a alcanzar un territorio rural ordenado. Los modelos territoriales de potencialidad o aptitud para las especies son herramientas que permiten a los gestores, agricultores y selvicultores desarrollar dicha planificación y tomar decisiones fundamentadas en un conocimiento científico lo más riguroso posible. Utilizando un método basado en la teoría de campos aplicada a la ecología, habitual para elaborar las áreas potenciales de especies forestales vegetales, se ha determinado el territorio climáticamente apto para T. melanosporum en la provincia Teruel. Para construir este modelo se han utilizado 924 localizaciones de la ecorregión Catalano-Aragonesa. Elaborando ocho parámetros climáticos trascendentes para la fructificación de la trufa negra, se han construido sus correspondientes hábitats. El modelo de potencialidad climática elaborado, completado con una corrección litológica, ha permitido identificar algo más de 8.000 km2 como aptos para la recolección de la trufa negra en Teruel, casi el 55% de la provincia, de los cuales 3.000 corresponden a la clase óptima y están distribuidos por las comarcas de Gúdar-Javalambre, Comunidad de Teruel y Maestrazgo. La adaptación del modelo ha permitido identificar más de 4.000 km2 como aptos para la instalación de plantaciones truferas, 1.150 de los cuales tienen potencialidad óptima. El modelo generado en el presente trabajo permitirá la adecuada planificación territorial de la truficultura en la provincia de Teruel

Gamificación educativa, control de asistencia y evaluación continua mediante Kahoot en clases teóricas y prácticas

Memoria ID-107. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019

EVALUACIÓN DEL CONOCIMIENTO ENFERMERO EN EL MANEJO AL PACIENTE NEUTROPÉNICO

Objective: To evaluate the nursing staff's knowledge about management of cancer patients with febrile neutropenia. Methodology: An observational descriptive study was carried out with 35 nurses of hospital centers in Havana city, from 2006 to 2008. A structured survey was designed and then applied, with previous consent, to the participating nurses. Results: Out of 35 nurses interviewed, 51.4% have their degree, 54.4% work in institutes, 57.1% perform assistance functions and 37.7% work in oncohematologic services. Work experience in this service is between one and two years. 68.5% of nurses know a lot about neutropenia, 25.7 % know a little, and 5.7 % do not know anything about the topic. Conclusions: The knowledge level was high in the majority, and it was associated with the precedent of having received postgraduate courses regarding Oncology and training from the nurses in the units where they work.Objetivo: Evaluar el conocimiento del personal de enfermería sobre el manejo del paciente oncológico con neutropenia febril. Método: Estudio observacional descriptivo evaluativo con 35 enfermeros pertenecientes a centros hospitalarios de Ciudad de la Habana durante el período 2006 -2008. Se diseño un cuestionario estructurado que fue aplicado con previo consentimiento de los enfermeros participantes. Resultados: De los 35 enfermeros encuestados, el 51.4% son licenciados, 54.4% trabajan en institutos, el 57.1% ejercen funciones asistenciales, y el 37.7% laboran en servicios oncohematologicos. Los años de experiencia de trabajo en el servicio oscilan entre 1 y 2 años. El 68.5% posee mucho conocimiento sobre neutropenia, poco un 25.7% y no tienen conocimientos el 5.7%. Conclusiones: El nivel de conocimiento fue evaluado en su mayoría de mucho, y se asoció al antecedente de haber recibido cursos postgrados sobre Oncología y adiestramientos de los enfermeros en los servicios donde laboran

Pulsational frequencies of the eclipsing delta-Scuti star HD 172189

The eclipsing delta-Scuti star HD 172189 is a probable member of the open cluster IC 4756 and a promising candidate target for the CoRoT mission. The detection of pulsation modes is the first step in the asteroseismological study of the star. Further, the calculation of the orbital parameters of the binary system allows us to make a dynamical determination of the mass of the star, which works as an important constraint to test and calibrate the asteroseismological models. From a detailed frequency analysis of 210 hours of photometric data of HD 172189 obtained from the STEPHI XIII campaign we have identified six pulsation frequencies with a confidence level of 99% and a seventh with a 65% confidence level in the range between 100-300 uHz. In addiction, three eclipses were observed during the campaign, allowing us to improve the determination of the orbital period of the system.Comment: 6 pages, 7 figure

New compound sets identified from high throughput phenotypic screening against three kinetoplastid parasites:an open resource

Using whole-cell phenotypic assays, the GlaxoSmithKline high-throughput screening (HTS) diversity set of 1.8 million compounds was screened against the three kinetoplastids most relevant to human disease, i.e. Leishmania donovani, Trypanosoma cruzi and Trypanosoma brucei. Secondary confirmatory and orthogonal intracellular anti-parasiticidal assays were conducted, and the potential for non-specific cytotoxicity determined. Hit compounds were chemically clustered and triaged for desirable physicochemical properties. The hypothetical biological target space covered by these diversity sets was investigated through bioinformatics methodologies. Consequently, three anti-kinetoplastid chemical boxes of ~200 compounds each were assembled. Functional analyses of these compounds suggest a wide array of potential modes of action against kinetoplastid kinases, proteases and cytochromes as well as potential host–pathogen targets. This is the first published parallel high throughput screening of a pharma compound collection against kinetoplastids. The compound sets are provided as an open resource for future lead discovery programs, and to address important research questions.The support and funding of Tres Cantos Open Lab Foundation is gratefully acknowledgedPeer reviewe

Estimation of photovoltaic potential for electricity self-sufficiency: A study case of military facilities in northwest Spain

Renewable energies, including photovoltaic energy, are attracting widespread international attention, in reaction to worsening environmental problems and the diminishing long-term sustainability of fossil fuel energies. In this work, the potential benefits of installing photovoltaic panels on several buildings at the Spanish Naval Military School (Escuela Naval Militar, ENM) of Mar ın are considered. The two salient advantages are significant economic savings from the production and the sale of electricity to the Spanish Electricity Network and achieving selfsufficiency in electricity requirements. Consequently, the main objective of this work is to estimate the energy potential of photovoltaic installations on the roofs of the ENM buildings. This is the first time that a project of this nature and size is presented to the Spanish Navy. To that end, a three-dimensional geographic analysis of the buildings is performed using three freeware software: Trimble SketchUp, Skelion, and Photovoltaic Geographical Information System. An economic study is also conducted to determine the feasibility of the installations, by estimating the Net Present Value of the photovoltaic installation and the Internal Rate of Return associated with the project. Subsequently, a sensitivity analysis that considers the most important parameters for the calculation of the amortization period is reported. The results show that the installation could fulfill the ENM electrical demands and could, in addition, generate significant economic benefits. The conclusions end with a recommendation to consider the merits of the proposed solution.Regional Government of Castilla y Le on (Ref. BU034U16), under European Regional Development Fund, and the Spanish Ministry of Economy, Industry and Competitiveness under the IþD þ i state programme Challenges for the Society (Ref. ENE-2014-54601-R). One of the authors, David Gonz alez Pe~na, thanks Junta de Castilla-Le on for economic support (PIRTU Program, ORDEN EDU/301/2015

Impact of Liver Inflammation on Bile Acid Side Chain Shortening and Amidation

Bile acid (BA) synthesis from cholesterol by hepatocytes is inhibited by inflammatory cytokines. Whether liver inflammation also affects BA side chain shortening and conjugation was investigated. In human liver cell lines (IHH, HepG2, and HepaRG), agonists of nuclear receptors including the farnesoid X receptor (FXR), liver X receptor (LXR), and peroxisome proliferator-activated receptors (PPARs) did not affect the expression of BA-related peroxisomal enzymes. In contrast, hepatocyte nuclear factor 4? (HNF4?) inhibition down-regulated acyl-CoA oxidase 2 (ACOX2). ACOX2 was repressed by fibroblast growth factor 19 (FGF19), which was prevented by extracellular signal-regulated kinase (ERK) pathway inhibition. These changes were paralleled by altered BA synthesis (HPLC-MS/MS). Cytokines able to down-regulate cholesterol-7?-hydroxylase (CYP7A1) had little effect on peroxisomal enzymes involved in BA synthesis except for ACOX2 and bile acid-CoA:amino acid N-acyltransferase (BAAT), which were down-regulated, mainly by oncostatin M (OSM). This effect was prevented by Janus kinase (JAK) inhibition, which restored BA side chain shortening and conjugation. The binding of OSM to the extracellular matrix accounted for a persistent effect after culture medium replacement. In silico analysis of four databases (n = 201) and a validation cohort (n = 90) revealed an inverse relationship between liver inflammation and ACOX2/BAAT expression which was associated with changes in HNF4? levels. In conclusion, BA side chain shortening and conjugation are inhibited by inflammatory effectors. However, other mechanisms involved in BA homeostasis counterbalance any significant impact on the serum BA profile

Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation

Introduction Periodontitis is a complex pathology characterized by the loss of alveolar bone. The causes and the mechanisms that promote this bone resorption still remain unknown. The knowledge of the critical regulators involved in the alteration of alveolar bone homeostasis is of great importance for developing molecular therapies. Procaine is an anesthetic drug with demethylant properties, mainly used by dentists in oral surgeries. The inhibitor role of Wnt signaling of procaine was described in vitro in colon cancer cells. Methods In this work we evaluated the role of procaine (1 uM) in osteo/odontogenesis of rat bone marrow mesenchymal stem cells. Similarly, the mechanisms whereby procaine achieves these effects were also studied. Results Procaine administration led to a drastic decrease of calcium content, alkaline phosphatase activity, alizarin red staining and an increase in the expression of Matrix Gla Protein. With respect to osteo/odontogenic markers, procaine decreased early and mature osteo/odontogenic markers. In parallel, procaine inhibited canonical Wnt/β-catenin pathway, observing a loss of nuclear β-catenin, a decrease in Lrp5 and Frizzled 3, a significant increase of sclerostin and Gsk3β and an increase of phosphorylated β-catenin. The combination of osteo/ odontogenic stimuli and Lithium Chloride decreased mRNA expression of Gsk3β, recovered by Procaine. Furthermore it was proved that Procaine alone dose dependently increases the expression of Gsk3β and β-catenin phosphorylation. These effects of procaine were also observed on mature osteoblast. Interestingly, at this concentration of procaine no demethylant effects were observed. PLO