Estimation of the atmospheric flux of nutrients and trace metals to the Eastern Tropical North Atlantic Ocean

Atmospheric deposition contributes potentially significant amounts of the nutrients iron, nitrogen and phosphorus (via mineral dust and anthropogenic aerosols) to the oligotrophic tropical North Atlantic Ocean. Transport pathways, deposition processes and source strengths contributing to this atmospheric flux are all highly variable in space and time. Atmospheric sampling was conducted during 28 research cruises through the Eastern Tropical North Atlantic (ETNA) over a 12 year period and a substantial dataset of measured concentrations of nutrients and trace metals in aerosol and rainfall over the region was acquired. This database was used to quantify (on a spatial- and seasonal-basis) the atmospheric input of ammonium, nitrate, soluble phosphorus and soluble and total iron, aluminium and manganese to the ETNA. The magnitude of atmospheric input varies strongly across the region, with high rainfall rates associated with the Inter-tropical Convergence Zone contributing to high wet deposition fluxes in the south, particularly for soluble species. Dry deposition fluxes of species associated with mineral dust exhibited strong seasonality, with highest fluxes associated with winter-time low-level transport of Saharan dust. Overall (wet plus dry) atmospheric inputs of soluble and total trace metals were used to estimate their soluble fractions. These also varied with season and were generally lower in the dry north than in the wet south. The ratio of ammonium plus nitrate to soluble iron in deposition to the ETNA was lower than the N:Fe requirement for algal growth in all cases, indicating the importance of the atmosphere as a source of excess iron

Flight Experiments on the Effects of Contamination on Electron Emission of Materials

We report on a study of the effects of prolonged exposure to the space environment and of chargeenhanced contamination on the electron emission and resistivity of spacecraft materials. The State of Utah Space Environment & Contamination Study (SUSpECS) was deployed on the International Space Station (ISS) in March 2008 onboard the MISSE-6 payload during STS-123. The Materials International Space Station Experiment (MISSE-6) program is designed to characterize the performance of candidate new space materials over the course of its ~17 month exposure to the LEO environment, with a target return date of August 2009 on STS-127. Approximately 165 samples are mounted on three separate SUSpECS panels in the ram and wake sides on the ISS. They have been carefully chosen to provide needed information for different ongoing studies and a broad cross-section of prototypical materials used on the exteriors of spacecrafts. Design of the sample panels are detailed, including a three tiered configuration intended to provide variable atomic oxygen and ultraviolet radiation exposure. The methods used to simulate charge enhanced contamination by actively biasing samples to low positive and negative voltages are also described. A primary emphasis of SUSpECS is the study of modifications to the electron emission resulting from exposure to the space plasma environment and to environmental contamination. There is presently little available data related to the effects of sample deterioration and contamination on emission properties for materials actually flown in space. Electron emission and transport properties of materials are key in determining the amount of charge build-up and the time for the charge to dissipate, as well as the likelihood of deleterious spacecraft charging effects. Such materials properties are essential parameters in modeling spacecraft charging with engineering tools like NASCAP-2K code. SUSpECS studies will test the validity of our predictions from ground-based studies that very thin layers of contamination can lead to severe charging effects under certain circumstance. Electron-, ion-, and photon-induced electron emission yield curves, crossover energies and emission spectra, as well as resistivity and dielectric strength, have been tested for most SUSpECS samples in their pristine conditions before flight. These measurements will be compared with post-flight measurements. Additional pre- and post-flight characterization measurements include optical and electron microscopy, reflection spectroscopy, emissivity and Auger electron spectroscopy

Faculty Recital: Justin Vickers, Tenor R. Kent Cook & Geoffrey Duce, Piano

Center for Performing Arts Concert Hall September 1, 2017 8:00p.m

Atmospheric trace metal concentrations, solubility and deposition fluxes in remote marine air over the south-east Atlantic

Total and soluble trace metal concentrations were determined in atmospheric aerosol and rainwater samples collected during seven cruises in the south-east Atlantic. Back trajectories indicated the samples all represented remote marine air masses, consistent with climatological expectations. Aerosol trace metal loadings were similar to previous measurements in clean, marine air masses. Median total Fe, Al, Mn, V, Co and Zn concentrations were 206, 346, 5, 3, 0.7 and 11 pmol m-3 respectively. Solubility was operationally defined as the fraction extractable using a pH4.7 ammonium acetate leach. Median soluble Fe, Al, Mn, V, Co, Zn, Cu, Ni, Cd and Pb concentrations were 6, 55, 1, 0.7, 0.06, 24, 2, 1, 0.05 and 0.3 pmol m-3 respectively. Large ranges in fractional solubility were observed for all elements except Co; median solubility values for Fe, Al and Mn were below 20% while the median for Zn was 74%. Volume weighted mean rainwater concentrations were 704, 792, 32, 10, 3, 686, 25, 0.02, 0.3 and 10 nmol L-1 for Fe, Al, Mn, V, Co, Zn, Cu, Ni, Cd and Pb respectively (n = 6). Wet deposition fluxes calculated from these values suggest rain makes a significant contribution to total deposition in the study area for all elements except perhaps Ni

In vitro anthelmintic effects of cysteine proteinases from plants against intestinal helminths of rodents

Infections with gastrointestinal (GI) nematodes are amongst the most prevalent worldwide, especially in tropical climates. Control of these infections is primarily through treatment with anthelmintic drugs, but the rapid development of resistance to all the currently available classes of anthelmintic means that alternative treatments are urgently required. Cysteine proteinases from plants such as papaya, pineapple and fig are known to be substantially effective against three rodent GI nematodes, Heligmosomoides polygyrus, Trichuris muris and Protospirura muricola, both in vitro and in vivo. Here, based on in vitro motility assays and scanning electron microscopy, we extend these earlier reports, demonstrating the potency of this anthelmintic effect of plant cysteine proteinases against two GI helminths from different taxonomic groups – the canine hookworm, Ancylostoma ceylanicum, and the rodent cestode, Rodentolepis microstoma. In the case of hookworms, a mechanism of action targeting the surface layers of the cuticle indistinguishable from that reported earlier appears to be involved, and in the case of cestodes, the surface of the tegumental layers was also the principal location of damage. Hence, plant cysteine proteinases have a broad spectrum of activity against intestinal helminths (both nematodes and cestodes), a quality that reinforces their suitability for development as a muchneeded novel treatment against GI helminths of humans and livestock

Usability of mobile applications: literature review and rationale for a new usability model

The usefulness of mobile devices has increased greatly in recent years allowing users to perform more tasks in amobile context. This increase in usefulness has come at the expense of the usability of these devices in somecontexts. We conducted a small review of mobile usability models and found that usability is usually measured interms of three attributes; effectiveness, efficiency and satisfaction. Other attributes, such as cognitive load, tend tobe overlooked in the usability models that are most prominent despite their likely impact on the success or failureof an application. To remedy this we introduces the PACMAD (People At the Centre of Mobile ApplicationDevelopment) usability model which was designed to address the limitations of existing usability models whenapplied to mobile devices. PACMAD brings together significant attributes from different usability models inorder to create a more comprehensive model. None of the attributes that it includes are new, but the existingprominent usability models ignore one or more of them. This could lead to an incomplete usability evaluation.We performed a literature search to compile a collection of studies that evaluate mobile applications and thenevaluated the studies using our model

Recapitulating Parkinson's disease pathology in a three-dimensional human neural cell culture model.

Extensive loss of dopaminergic neurons, and aggregation of the protein α-synuclein into ubiquitin-positive Lewy bodies represents a major neuropathological hallmark of Parkinson's disease. At present the generation of large nuclear-associated Lewy bodies from endogenous wild-type α-synuclein, translationally regulated under its own promoter in human cell culture models requires costly and time-consuming protocols. Here, we demonstrate that fully differentiated human SH-SY5Y neuroblastoma cells grown in three-dimensional cell culture develop Lewy body-like pathology upon exposure to exogenous α-synuclein species. In contrast to most cell- and rodent-based models that exhibit multiple diffuse α-synuclein aggregates throughout the cytoplasm, a single large nuclear inclusion immuno-positive for α-synuclein and ubiquitin is rapidly obtained in our model. This was achieved, without the need for over-expression of α-synuclein or genetic modification of the cell line. However, phosphorylation of α-synuclein within these inclusions was not observed. The system described here provides an ideal tool to screen compounds to therapeutically intervene in Lewy body formation and to investigate the mechanisms involved in disease progression in synucleinopathies

Cortical AAV-CNTF gene therapy combined with intraspinal mesenchymal precursor cell transplantation promotes functional and morphological outcomes after spinal cord injury in adult rats

Ciliary neurotrophic factor (CNTF) promotes survival and enhances long-distance regeneration of injured axons in parts of the adult CNS. Here we tested whether CNTF gene therapy targeting corticospinal neurons (CSN) in motor-related regions of the cerebral cortex promotes plasticity and regrowth of axons projecting into the female adult F344 rat spinal cord after moderate thoracic (T10) contusion injury (SCI). Cortical neurons were transduced with a bicistronic adeno-associated viral vector (AAV1) expressing a secretory form of CNTF coupled to mCHERRY (AAV-CNTFmCherry) or with control AAV only (AAV-GFP) two weeks prior to SCI. In some animals, viable or nonviable F344 rat mesenchymal precursor cells (rMPCs) were injected into the lesion site two weeks after SCI to modulate the inhibitory environment. Treatment with AAV-CNTFmCherry, as well as with AAV-CNTFmCherry combined with rMPCs, yielded functional improvements over AAV-GFP alone, as assessed by open-field and Ladderwalk analyses. Cyst size was significantly reduced in the AAV-CNTFmCherry plus viable rMPC treatment group. Cortical injections of biotinylated dextran amine (BDA) revealed more BDA-stained axons rostral and alongside cysts in the AAV-CNTFmCherry versus AAV-GFP groups. After AAV-CNTFmCherry treatments, many sprouting mCherry-immunopositive axons were seen rostral to the SCI, and axons were also occasionally found caudal to the injury site. These data suggest that CNTF has the potential to enhance corticospinal repair by transducing parent CNS populations