MULTIVARIATE ANALYSIS OF GENETIC DIVERGENCE AMONG NIGER GENOTYPES IN RELATION TO SEED OIL QUALITY TRAITS

A study was conducted to assess the extent and pattern of genetic variability of niger genotypes with respect to twelve seed oil quality traits in forty niger genotypes. The analysis of variances has shown that there was significant variation among the genotypes in all traits. The high heritability (>60%) was recorded for all oil parameters except for gamma tocopherol (2.80%). The multivariate analysis has resulted in formation of eleven clusters. Out of eleven clusters formed, cluster 1 was the largest with eighteen genotypes followed by cluster 4 with six genotypes. The highest genetic distance was recorded between cluster 7 and 11 (28.19), while lowest genetic distances noticed between cluster 6 and 7 (8.24).  Cluster 2 included four genotypes with highest means for oil content (39.83), protein (33.51), oleic acid (36.53), stearic acid (10.31), alpha tocopherol (94.42) and gamma tocopherol (7.50). Thus, on the basis of present finding there is a possibility of simultaneously improving the fatty acid profile, tocopherol as well as the oil content with seed yield per plant of the genotypes through inter crossing between genotypes viz., JNS-164, JNS-165, Utakal Niger -150, GA-10, IGPN-08-66, IGPN-2004-1, JNS-501, IGPN-8004 and BAU-10-5. Among twelve traits studied palmatic acid (28.97%) contributed highest towards the genetic divergence followed by linoleic acid (27.44%) and stearic acid (18.46%)

INDUCTION OF SOMATIC MUTATION IN CHRYSANTHEMUM CULTIVAR LOCAL GOLDEN

The rooted sucker of chrysanthemum cultivar “Local Golden” was treated with 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 Krad of gamma rays. Each treatment consisted of 25 suckers. The data were recorded on vegetative and flower traits i.e. survival percent, days for sprouting, plant height, internodal distance, number of branches per plant, number of sucker per plant, total crop duration. Flowering behavior (days to bud initiation, days to full bloom, diameter of flower, number of ray florets per flower, number of flower per plant, colour of ray and disc florets). There were no leaf and floral abnormalities in treated plants. LD50 was determined in between 2.5 and 3.0 Krad dose of gamma rays. There was no significant difference in colour of florets of treated and control plants. Chimera in ray florets of flower was observed in one plant after 1.5 Krad treatments. The original shape of ray florets was flat with small tube at the base whereas in case of tubular mutant, shape at tip was spoon type and basal portion showed pipe or tube like appearance

Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent.

Biosurfactants are amphipathic, surface-active molecules of microbial origin which accumulate at interfaces reducing interfacial tension and leading to the formation of aggregated micellular structures in solution. Some biosurfactants have been reported to have antimicrobial properties, the ability to prevent adhesion and to disrupt biofilm formation. We investigated antimicrobial properties and biofilm disruption using sophorolipids at different concentrations. Growth of Gram negative Cupriavidus necator ATCC 17699 and Gram positive Bacillus subtilis BBK006 were inhibited by sophorolipids at concentrations of 5% v/v with a bactericidal effect. Sophorolipids (5% v/v) were also able to disrupt biofilms formed by single and mixed cultures of B. subtilis BBK006 and Staphylococcus aureus ATCC 9144 under static and flow conditions, as was observed by scanning electron microscopy. The results indicated that sophorolipids may be promising compounds for use in biomedical application as adjuvants to other antimicrobial against some pathogens through inhibition of growth and/or biofilm disruption

Formulation Develoment And Evaluation Herbal Effervescent Floating Tablet By Using Syzygium Cumini Seed Extract Used In Treatment Of Diabetes

Background: Floating tablets prolong the gastric residence time of drugs, improves bioavailability, and facilitate local drug delivery to the stomach. With this objective, floating tablets containing extract of Syzgium cumini seed extract as active ingredient was prepared for the treatment of antidiabetic.  Material and method: Floating tablets of Syzgium cumini seed extract were prepared by direct compression method using Magnesium stearate, Microcrystalline cellulose, Citric acid and Sucrose. The formulations were evaluated for various physical parameters, floating lagtime. Result: The thickness was in the range 4.02-4.086 mm. The hardness ranged from 3.1-3.3 kg/cm2, All formulations passed the USP requirements for friability and uniformity of weight. The buoyancy time of all tablet formulations was less than 5min and tablet remained in floating condition throughout the study. Conclusion: The optimized formulation was found to be F5 batch which released 98.13% of drug in 8hr invitro, while the floating lag time was 92 seconds

Formulation Development and Evaluation of Leaf Extract of Ficus benghalensis for Antidiabetic Activity

Herbal products are known for their inherent property i.e. comparatively safe and economic. In present study, leaf extract of ficus benghalensis was evaluated for antidiabetic activity. The aim of the research work was to formulate and evaluate capsule dosage form of ethanolic extract. Leaves of Ficus benghalensis collected from local area of Ahmednagar district and shade dried. Ethanolic, Hydroalcoholic and petroleum ether extracts were prepared using soxhlet apparatus. Extracts were screened for antidiabetic activity using alloxan induced diabetes in rats. Oral glucose tolerance test was measured as parameter to check antidiabetic activity. Ethanolic extract was found most effective among them. Granules were prepared using ethanolic ectract and filled in capsule.  Capsule were evaluated for parameters including uniformity of weight, disintegration time. Keywords: Ficus benghalensis, Ethanolic extract, Antidiabetic activit

Disseminating technology in global surgery.

BACKGROUND:Effective dissemination of technology in global surgery is vital to realize universal health coverage by 2030. Challenges include a lack of human resource, infrastructure and finance. Understanding these challenges, and exploring opportunities and solutions to overcome them, are essential to improve global surgical care. METHODS:This review focuses on technologies and medical devices aimed at improving surgical care and training in low- and middle-income countries. The key considerations in the development of new technologies are described, along with strategies for evaluation and wider dissemination. Notable examples of where the dissemination of a new surgical technology has achieved impact are included. RESULTS:Employing the principles of frugal and responsible innovation, and aligning evaluation and development to high scientific standards help overcome some of the challenges in disseminating technology in global surgery. Exemplars of effective dissemination include low-cost laparoscopes, gasless laparoscopic techniques and innovative training programmes for laparoscopic surgery; low-cost and versatile external fixation devices for fractures; the LifeBox pulse oximeter project; and the use of immersive technologies in simulation, training and surgical care delivery. CONCLUSION:Core strategies to facilitate technology dissemination in global surgery include leveraging international funding, interdisciplinary collaboration involving all key stakeholders, and frugal scientific design, development and evaluation

A new species of genus Amoebotaenia alokni sp. nov. From Gallus gallus domesticus from Ahmednagar district, (MS) India

Present communication deals with taxonomical studies to identified and describe a new species, about forty nine cestode parasites were collected from the gastrointestinal tract of Gallus gallus domesticus from different villages of Ahmednagar district for taxonomical studies. The genus Amoebotaenia was erected by Cohn L. in the year 1900, since from 1900 about 19 species are added in Amoebotaenia genus. After going through the literature the identified Amoebotaenia alokni n. sp. parasite is differ from the known species of the genus Amoebotaenia in distinct and differentiating characters like shape and size of the scolex, number of hook, testes, Proglottids and Shape and Size Ovary etc. Some additional characters are given in comparative chart at the end. These distinct characters are more than enough to erected a new species from this genus and hence the name Amoebotaenia alokni n.sp</jats:p

Clinicoinvestigative profile of ischemic heart disease patients admitted in tertiary care teaching hospital: A cross-sectional study

Background: Despite considerable progress in the diagnosis, management, and prevention of cardiovascular diseases, they are still the main cause of death globally. These diseases have assumed epidemic proportions in India as well. The angiographic profile of coronary artery disease patients, its clinical presentation, and risk factors differ worldwide. Aim and Objective: The aim of the study was to study the clinicoinvestigative profile of ischemic heart disease (IHD) patients seeking treatment at tertiary care teaching hospital of western Maharashtra. Materials and Methods: Institutional Ethics Committee approval was sought before initiation of the study. The present, descriptive, and cross-sectional study was conducted on IHD patients admitted for coronary angiography in cardiac recovery unit of tertiary care hospital. Detailed clinical data such as signs and symptoms, associated diseases, general, and systemic examination findings were collected. Results: Out of total 190 patients, 61.57% (117) were males and 38.42% (73) were females. Majority patients were between 41 and 60 years (78.94%) of age group. The most common chief complaint was chest pain (72%), followed by breathlessness (28%), palpitation (12%) sweating (18%), and vomiting (08%). Out of all 77.89% (148) had ST-segment elevation myocardial infarction (STEMI) while other hand 16.84% (32) and 04.73% (09) had non-ST-segment elevation myocardial and unstable angina, respectively. Conclusion: The present study concluded that IHD is more prevalent in males than females. STEMI was more common clinical presentation and cardiac failure (69.09%) was more prevalent complication among IHD.</jats:p