Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution

High-throughput functional analysis of autism genes in zebrafish identifies convergence in dopaminergic and neuroimmune pathways

Advancing from gene discovery in autism spectrum disorders (ASDs) to the identification of biologically relevant mechanisms remains a central challenge. Here, we perform parallel in vivo functional analysis of 10 ASD genes at the behavioral, structural, and circuit levels in zebrafish mutants, revealing both unique and overlapping effects of gene loss of function. Whole-brain mapping identifies the forebrain and cerebellum as the most significant contributors to brain size differences, while regions involved in sensory-motor control, particularly dopaminergic regions, are associated with altered baseline brain activity. Finally, we show a global increase in microglia resulting from ASD gene loss of function in select mutants, implicating neuroimmune dysfunction as a key pathway relevant to ASD biology

Predicting Hemolytic Uremic Syndrome and Renal Replacement Therapy in Shiga Toxin-producing Escherichia coli-infected Children.

BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) infections are leading causes of pediatric acute renal failure. Identifying hemolytic uremic syndrome (HUS) risk factors is needed to guide care. METHODS: We conducted a multicenter, historical cohort study to identify features associated with development of HUS (primary outcome) and need for renal replacement therapy (RRT) (secondary outcome) in STEC-infected children without HUS at initial presentation. Children agedeligible. RESULTS: Of 927 STEC-infected children, 41 (4.4%) had HUS at presentation; of the remaining 886, 126 (14.2%) developed HUS. Predictors (all shown as odds ratio [OR] with 95% confidence interval [CI]) of HUS included younger age (0.77 [.69-.85] per year), leukocyte count ≥13.0 × 103/μL (2.54 [1.42-4.54]), higher hematocrit (1.83 [1.21-2.77] per 5% increase) and serum creatinine (10.82 [1.49-78.69] per 1 mg/dL increase), platelet count \u3c250 \u3e× 103/μL (1.92 [1.02-3.60]), lower serum sodium (1.12 [1.02-1.23 per 1 mmol/L decrease), and intravenous fluid administration initiated ≥4 days following diarrhea onset (2.50 [1.14-5.46]). A longer interval from diarrhea onset to index visit was associated with reduced HUS risk (OR, 0.70 [95% CI, .54-.90]). RRT predictors (all shown as OR [95% CI]) included female sex (2.27 [1.14-4.50]), younger age (0.83 [.74-.92] per year), lower serum sodium (1.15 [1.04-1.27] per mmol/L decrease), higher leukocyte count ≥13.0 × 103/μL (2.35 [1.17-4.72]) and creatinine (7.75 [1.20-50.16] per 1 mg/dL increase) concentrations, and initial intravenous fluid administration ≥4 days following diarrhea onset (2.71 [1.18-6.21]). CONCLUSIONS: The complex nature of STEC infection renders predicting its course a challenge. Risk factors we identified highlight the importance of avoiding dehydration and performing close clinical and laboratory monitoring

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Alabama DOE/EPSCoR traineeship program. Final report, September 28, 1991--September 28, 1995

This report covers programmatic accomplishments of the Alabama DOE/EPSCoR Traineeship Program for the period September 28, 1991 to September 29, 1995. The Alabama DOE/EPSCoR Traineeship Program is an integral part of this state`s efforts to address barriers that inhibit the full development and substantial growth of energy-related research at the six major research institutions and at Alabama`s Historically Black Colleges and Universities (HBCUs). To overcome these barriers it was determined that the following actions were needed: Area 1: Strengthening the Research Faculty Base Area 2: Increasing the Number of Outstanding Graduate Students Area 3: Improving the Research Environment Area 4: Developing the Human Resources Base Area 5. Improving the Energy-related Infrastructure, Collaborations and Communications. Although the DOE/EPSCoR Traineeship Grant complements each of the areas listed above, its primary emphasis is the enhancement of opportunities for graduate students. The extent to which this program has met this challenge during the three year funding period constitutes the substance of this report

Prevalence of speech language impairment in African American families

Poster presentation at the annual convention of the American Speech Language Hearing Association, Boston, MA

Grammatical Judgment Tasks: Evidence From AAE Speakers

Poster presentation at the annual convention of the American Speech Language Hearing Association, San Diego, CA

Family History of Speech and Language Impairment in African American Children: Implications for Assessment

Purpose We explored the prevalence of a positive family history of speech and language impairment in African American children as a function of their socioeconomic status (SES), receipt of speech–language services, and diagnosis of specific language impairment (SLI). Method Data were collected in 2 phases. Phase 1 included family questionnaires from 161 kindergartners. Phase 2 included interviews with the primary caregivers of 17 of these kindergartners. Results Overall, the prevalence of a positive family history was 24%. Children receiving services did not present a higher rate of positive family history than children not receiving services, but low-SES children were 2 times more likely than middle-SES children to present positive family histories. Children with SLI were also 2 times more likely to present a positive family history than children with typical development, and after controlling for SES, elevated rates of a positive family history for those with SLI remained. Conclusions Results support studies that have found higher rates of positive family history in children with SLI relative to controls while also highlighting SES as an important variable to consider within family history studies. These findings call for careful consideration of family history and SES information when assessing African American children

Support of the IPSyn for children who speak AAE

Poster presentation at the annual convention of the American Speech Language Hearing Association, Chicago, IL