Hier ist wahrhaftig ein Loch im Himmel - The NGC 1999 dark globule is not a globule

The NGC 1999 reflection nebula features a dark patch with a size of ~10,000 AU, which has been interpreted as a small, dense foreground globule and possible site of imminent star formation. We present Herschel PACS far-infrared 70 and 160mum maps, which reveal a flux deficit at the location of the globule. We estimate the globule mass needed to produce such an absorption feature to be a few tenths to a few Msun. Inspired by this Herschel observation, we obtained APEX LABOCA and SABOCA submillimeter continuum maps, and Magellan PANIC near-infrared images of the region. We do not detect a submillimer source at the location of the Herschel flux decrement; furthermore our observations place an upper limit on the mass of the globule of ~2.4x10^-2 Msun. Indeed, the submillimeter maps appear to show a flux depression as well. Furthermore, the near-infrared images detect faint background stars that are less affected by extinction inside the dark patch than in its surroundings. We suggest that the dark patch is in fact a hole or cavity in the material producing the NGC 1999 reflection nebula, excavated by protostellar jets from the V 380 Ori multiple system.Comment: accepted for the A&A Herschel issue; 7 page

Oscillations in the massive Wolf-Rayet star WR 123 with the MOST satellite

Publisher's version/PDFWe present the results of intensive visual-broadband photometric monitoring of the highly variable WN8 Wolf-Rayet star WR 123, obtained by the MOST (Microvariability and Oscillations of STars) satellite. This first Canadian astronomical space telescope observed WR 123 for 38 days nonstop during 2004 June and July. Fourier analysis shows that no periodic signal is stable for more than several days in the low-frequency domain (f [less than] 1 day[superscript -1]), where most of the stochastic power is contained. Also, no significant variability is seen in the high-frequency domain (10 day[superscript -1] [less than] f [less than] 1400 day[superscript -1]) down to the level of 0.2 mmag, an order of magnitude lower than theoretical predictions for strange-mode pulsations. On the other hand, there seems to be a relatively stable 9.8 hr periodic signal present throughout the whole run. This period is probably too short to represent the axial rotation of the star, unless it is related to multiple substructures equidistantly spread along the stellar equator. It is also too short to be orbital in nature; it is more likely to be related to pulsational instablilities [sic] (although with a much longer period than expected), thus finally revealing a possible fundamental driver behind the highly variable wind of this object, and others of similar type

The regulatory subunit of PKA-I remains partially structured and undergoes β-aggregation upon thermal denaturation

Background: The regulatory subunit (R) of cAMP-dependent protein kinase (PKA) is a modular flexible protein that responds with large conformational changes to the binding of the effector cAMP. Considering its highly dynamic nature, the protein is rather stable. We studied the thermal denaturation of full-length RIα and a truncated RIα(92-381) that contains the tandem cyclic nucleotide binding (CNB) domains A and B. Methodology/Principal Findings: As revealed by circular dichroism (CD) and differential scanning calorimetry, both RIα proteins contain significant residual structure in the heat-denatured state. As evidenced by CD, the predominantly α-helical spectrum at 25°C with double negative peaks at 209 and 222 nm changes to a spectrum with a single negative peak at 212-216 nm, characteristic of β-structure. A similar α→β transition occurs at higher temperature in the presence of cAMP. Thioflavin T fluorescence and atomic force microscopy studies support the notion that the structural transition is associated with cross-β-intermolecular aggregation and formation of non-fibrillar oligomers. Conclusions/Significance: Thermal denaturation of RIα leads to partial loss of native packing with exposure of aggregation-prone motifs, such as the B' helices in the phosphate-binding cassettes of both CNB domains. The topology of the β-sandwiches in these domains favors inter-molecular β-aggregation, which is suppressed in the ligand-bound states of RIα under physiological conditions. Moreover, our results reveal that the CNB domains persist as structural cores through heat-denaturation. © 2011 Dao et al

Multifrequency variability of the blazar AO 0235+164. The WEBT campaign in 2004-2005 and long-term SED analysis

A huge multiwavelength campaign targeting the blazar AO 0235+164 was organized by the Whole Earth Blazar Telescope (WEBT) in 2003-2005 to study the variability properties of the source. Monitoring observations were carried out at cm and mm wavelengths, and in the near-IR and optical bands, while three pointings by the XMM-Newton satellite provided information on the X-ray and UV emission. We present the data acquired during the second observing season, 2004-2005, by 27 radio-to-optical telescopes. They reveal an increased near-IR and optical activity with respect to the previous season. Increased variability is also found at the higher radio frequencies, down to 15 GHz, but not at the lower ones. The radio (and optical) outburst predicted to peak around February-March 2004 on the basis of the previously observed 5-6 yr quasi-periodicity did not occur. The analysis of the optical light curves reveals now a longer characteristic time scale of 8 yr, which is also present in the radio data. The spectral energy distributions corresponding to the XMM-Newton observations performed during the WEBT campaign are compared with those pertaining to previous pointings of X-ray satellites. Bright, soft X-ray spectra can be described in terms of an extra component, which appears also when the source is faint through a hard UV spectrum and a curvature of the X-ray spectrum. Finally, there might be a correlation between the X-ray and optical bright states with a long time delay of about 5 yr, which would require a geometrical interpretation.Comment: 14 pages, 10 figures (8 included in the text and 2 PNG files), in press for A&

Recruitment and Activation of RSK2 by HIV-1 Tat

The transcriptional activity of the integrated HIV provirus is dependent on the chromatin organization of the viral promoter and the transactivator Tat. Tat recruits the cellular pTEFb complex and interacts with several chromatin-modifying enzymes, including the histone acetyltransferases p300 and PCAF. Here, we examined the interaction of Tat with activation-dependent histone kinases, including the p90 ribosomal S6 kinase 2 (RSK2). Dominant-negative RSK2 and treatment with a small-molecule inhibitor of RSK2 kinase activity inhibited the transcriptional activity of Tat, indicating that RSK2 is important for Tat function. Reconstitution of RSK2 in cells from subjects with a genetic defect in RSK2 expression (Coffin-Lowry syndrome) enhanced Tat transactivation. Tat interacted with RSK2 and activated RSK2 kinase activity in cells. Both properties were lost in a mutant Tat protein (F38A) that is deficient in HIV transactivation. Our data identify a novel reciprocal regulation of Tat and RSK2 function, which might serve to induce early changes in the chromatin organization of the HIV LTR

Differential regulation of mitochondrial pyruvate carrier genes modulates respiratory capacity and stress tolerance in yeast

Mpc proteins are highly conserved from yeast to humans and are necessary for the uptake of pyruvate at the inner mitochondrial membrane, which is used for leucine and valine biosynthesis and as a fuel for respiration. Our analysis of the yeast MPC gene family suggests that amino acid biosynthesis, respiration rate and oxidative stress tolerance are regulated by changes in the Mpc protein composition of the mitochondria. Mpc2 and Mpc3 are highly similar but functionally different: Mpc2 is most abundant under fermentative non stress conditions and important for amino acid biosynthesis, while Mpc3 is the most abundant family member upon salt stress or when high respiration rates are required. Accordingly, expression of the MPC3 gene is highly activated upon NaCl stress or during the transition from fermentation to respiration, both types of regulation depend on the Hog1 MAP kinase. Overexpression experiments show that gain of Mpc2 function leads to a severe respiration defect and ROS accumulation, while Mpc3 stimulates respiration and enhances tolerance to oxidative stress. Our results identify the regulated mitochondrial pyruvate uptake as an important determinant of respiration rate and stress resistance.This work was supported by Ministerio de Economia y Competitividad grant BFU2011-23326 to M.P.; A.T.-G. was supported by a JAE predoctoral grant from Consejo Superior de Investigaciones Cientificas. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Timón Gómez, A.; Proft ., MH.; Pascual-Ahuir Giner, MD. (2013). Differential regulation of mitochondrial pyruvate carrier genes modulates respiratory capacity and stress tolerance in yeast. PLoS ONE. 8(11):1-9. doi:10.1371/journal.pone.0079405S19811Murphy, M. P. (2008). How mitochondria produce reactive oxygen species. 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Multifrequency variability of the blazar AO 0235+164 the WEBT campaign in 2004-2005 and long-term SED analysis

A huge multiwavelength campaign targeting the blazar AO 0235+164 was organized by the Whole Earth Blazar Telescope (WEBT) in 2003-2005 to study the variability properties of the source. Monitoring observations were carried out at cm and mm wavelengths, and in the near-IR and optical bands, while three pointings by the XMM-Newton satellite provided information on the X-ray and UV emission. We present the data acquired during the second observing season, 2004-2005, by 27 radio-to-optical telescopes. They reveal an increased near-IR and optical activity with respect to the previous season. Increased variability is also found at the higher radio frequencies, down to 15 GHz, but not at the lower ones. The radio (and optical) outburst predicted to peak around February-March 2004 on the basis of the previously observed 5-6 yr quasi-periodicity did not occur. The analysis of the optical light curves reveals now a longer characteristic time scale of 8 yr, which is also present in the radio data. The spectral energy distributions corresponding to the XMM-Newton observations performed during the WEBT campaign are compared with those pertaining to previous pointings of X-ray satellites. Bright, soft X-ray spectra can be described in terms of an extra component, which appears also when the source is faint through a hard UV spectrum and a curvature of the X-ray spectrum. Finally, there might be a correlation between the X-ray and optical bright states with a long time delay of about 5 yr, which would require a geometrical interpretation

The Effects of Alfalfa Seed Scarification in Saline Environments

The handling of alfalfa and other crop seed may result in seed scarification. Scarification may not affect germination of alfalfa seed in a non - saline environment, but may decrease germination where farmer's fields are severely salt-stressed

Carbohydrates in Germination Salt Tolerant and Non-Salt Tolerant Alfalfa Seed

Alfalfa which is extremely salt tolerant during germination has been developed by researchers at the University of Arizona Carbohydrates were analyzed in the original parental gemiplasm 'Mesa- Sirsa' and Cycle₅Syn₂ and Cycle₈Syn₂ seed to determine why this seed could germinate in extremely saline conditions. Raffinose and sucrose were both significantly higher in the salt -tolerant germplasm compared to the parental germplasm; however, the magnitude increase of these free sugars was not sufficient to explain the increased ability of the seed to absorb water in a stress environment. The galactomannan content was not different among the alfalfa germplasms