Anúncio publicitário à luz da Análise de Discurso Crítica / Advertising in accordance with Critical Discourse Analysis

O trabalho é resultado de uma proposta de atividade desenvolvida na disciplina Gêneros Discursivos e/ou Textuais nas Práticas Sociais, ministrada pela professora Dra. Maria Aparecida Resende Ottoni, do Programa de Pós-Graduação Mestrado Profissional em Letras – Profletras, pela Universidade Federal de Uberlândia no primeiro semestre de 2021, com o intuito de percebermos a viabilidade dessa análise na prática escolar. Fizemos uma reflexão acerca de um exemplar do gênero discursivo/textual anúncio publicitário da Renault Kwid Outsider sob a perspectiva da Análise de Discurso Crítica (ADC) cujo objetivo é fornecer instrumentos para desvelar as relações assimétricas de poder. O texto selecionado para nossa análise recria o famoso desenho animado Caverna do Dragão e tem como mote “Deixe o Impossível para Trás”. Para tanto, recorremos às considerações de Fairclough (2003) e Ottoni (2017). Para nossa análise foram selecionados alguns aspectos da ADC pertinentes ao exemplar escolhido: questões sobre o gênero, relações sociais, tecnologias de comunicação, relações semânticas, relações formais, modos operacionais e intertextualidade. Os resultados dessa análise mostraram que, por meio da ADC, é possível proporcionar aos indivíduos uma visão crítica das relações de poder nas práticas sociais

A modified hydrogel production protocol to decrease cellular content

PURPOSE: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. METHODS: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. RESULTS: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. CONCLUSIONS: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Preliminary comparative analysis of the acute toxicity and median lethal dose (LD50) of the fruit of the Brazilian black pepper (Schinus terebinthifolius Raddi) and black pepper (Piper nigrum L.)

No presente trabalho, procurou-se avaliar a toxicidade aguda comparativa entre duas espécies de pimentas extensamente utilizadas na culinária brasileira. A Schinus terebinthifolius Raddi (pimenta-doreino do Brasil) é encontrada com frequência em supermercados e outros estabelecimentos comerciais, sendo empregada como condimento ou até mesmo fraudando a tradicional pimenta-do-reino (Piper nigrum L.), porém sem a existência de trabalhos científicos que assegurem sua inocuidade ou riscos para o consumo humano. Para tanto os extratos brutos dos frutos de Schinus terebinthifolius Raddi e Piper nigrum L. foram administrados per os em dose única a camundongos em concentrações crescentes (2-5 g.Kg-1) para a avaliação de seu "Screening Hipocrático" e determinação da dose letal mediana (DL50) pela administração per os e intraperitoneal (ip). A administração per os revelou valor estimado de DL50 para ambas pimentas superior a 5 g.Kg-1, já pela via ip os resultados de DL50 para a Schinus terebinthifolius Raddi e Piper nigrum L. foram 3.5 e 3.714 g.Kg-1 respectivamente. A espécie Piper nigrum L. nas primeiras 12 h de análise do "Screening Hipocrático" evidenciou efeito depressor sobre os parâmetros atividade geral, resposta ao toque, reflexo de endireitamento e tônus do corpo, manifestações estas que reverteram espontâneamente nas primeiras 24 h de observação. Os resultados obtidos revelaram uma baixa toxicidade oral de ambas pimentas quando administradas a camundongos (5 g.Kg-1, per os), dose esta 2.500 vezes superior ao consumo diário pelo ser humano de Piper nigrum L.In the present paper, the acute toxicity of two species of pepper that are extensively used in Brazilian culinary was compared. Schinus terebinthifolius Raddi (Brazilian black pepper) is frequently found in supermarkets and other commercial establishments sold as a spice and even fraudulently in place of Pipper nigrum L, in spite of the absence of scientific papers determining whether it is innocuous or if there are risks for human consumption. For this reason, growing concentrations (2-5 g.Kg-1 ) of the crude extracts of the fruit of Schinus terebinthifolius Raddi and of Pipper nigrum L. were administered orally in a single dose to mice, in order to carry out their Hippocratic Screening and determine the average lethal dose (LD50) by oral (per os) and intraperitoneal (ip) administration. The LD50 by oral administration was estimated to be over 5 g Kg-1, whereas by ip administration the LD50 values for Schinus terebinthifolius Raddi and Pipper nigrum L, were found to be 3.5 and 3.714 g.Kg-1, respectively. In the first 12 h of the Hippocratic Screening, the species Pipper nigrum L. was shown to depress the general activity parameters, response to touch, righting reflex and body tonus; reverting spontaneously in the first 24 h of observation. The results obtained show that both peppers have a low oral toxicity, when administered to mice (5 g.Kg-1, per os) in doses which are 2.500 times greater that the daily human consumption of Pipper nigrum L.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Low-level light-emitting diode therapy increases mRNA expressions of IL-10 and type I and III collagens on Achilles tendinitis in rats

The present study investigated the effects of low-level light-emitting diode (LED) therapy (880 +/- 10 nm) on interleukin (IL)-10 and type I and III collagen in an experimental model of Achilles tendinitis. Thirty male Wistar rats were separated into six groups (n = 5), three groups in the experimental period of 7 days, control group, tendinitis-induced group, and LED therapy group, and three groups in the experimental period of 14 days, tendinitis group, LED therapy group, and LED group with the therapy starting at the 7th day after tendinitis induction (LEDT delay). Tendinitis was induced in the right Achilles tendon using an intratendinous injection of 100 mu L of collagenase. the LED parameters were: optical power of 22 mW, spot area size of 0.5 cm(2), and irradiation time of 170 s, corresponding to 7.5 J/cm(2) of energy density. the therapy was initiated 12 h after the tendinitis induction, with a 48-h interval between irradiations. the IL-10 and type I and III collagen mRNA expression were evaluated by real-time polymerase chain reaction at the 7th and 14th days after tendinitis induction. the results showed that LED irradiation increased IL-10 (p < 0.001) in treated group on 7-day experimental period and increased type I and III collagen mRNA expression in both treated groups of 7- and 14-day experimental periods (p < 0.05), except by type I collagen mRNA expression in LEDT delay group. LED (880 nm) was effective in increasing mRNA expression of IL-10 and type I and III collagen. Therefore, LED therapy may have potentially therapeutic effects on Achilles tendon injuries.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Vales Jequitinhonha & Mucuri, Dept Physiotherapy, Lab Res & Anim Experimentat, BR-39100000 Diamantina, MG, BrazilUniv Camilo Castelo Branco, Inst Biomed Engn, São Paulo, BrazilInst Fed Educ Ciencia & Tecnol Sul Minas Gerais, Grp Estudos & Pesquisa Ciencias Saude, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilNove de Julho Univ, UNINOVE, Dept Rehabil Sci, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilFAPEMIG: APQ-01733-11Web of Scienc

A modified hydrogel production protocol to decrease cellular content

Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. Conclusion: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%

Relações entre profissionais de saúde e usuários durante as práticas em saúde Relationships between health professionals and users throughout health care practices

Apresenta-se uma revisão integrativa sobre estudos que abordam as relações entre profissionais de saúde e usuários durante as práticas em saúde. Objetivou-se identificar os aspectos pesquisados no cotidiano dos serviços acerca dessas relações. A coleta foi realizada nas bases Lilacs e Pubmed segundo os descritores: acolhimento; relações profissional-família; relações profissional-paciente; humanização da assistência; e a palavra 'vínculo' associada ao descritor Sistema Único de Saúde. Selecionaram-se 290 estudos publicados entre 1990 e 2010. Por meio da análise temática, foram criados cinco núcleos de sentido: a relevância da confiança na relação profissional-usuário; sentimentos e sentidos na prática do cuidado; a importância da comunicação nos serviços de saúde; modo de organização das práticas em saúde; e (des)colonialismo. Identificou-se que as relações estabelecidas nas práticas de saúde têm uma dimensão transformadora. No entanto, permanece o desafio de humanizar os serviços de saúde. A enfermagem se destaca na produção do conhecimento nessa temática.<br>This article presents an integrative review about studies that address the relationships between health professionals and users in health care practices. It aimed to identify aspects that were researched on the daily life of the services concerning such relationships. Data were collected from the Lilacs and Pubmed databases based on these descriptors: user embracement; professionalfamily relations; professionalpatient relations; humanization of the care; and the bonding word associated to the Single Health System descriptor. Two hundred and ninety studies, published from 1990 to 2010, were selected. Through thematic analyses, five meaning cores were created: the relevance of the confidence in the professionaluser relationship; feelings and senses in the health care practice; the importance of communications in health care services; ways to organize health care practices and (de)colonialism. It was found that relationships established in health care practices have a transformative dimension. However, the challenge to humanize health care services remains. Nursing stands out in the production of knowledge on such theme

Neotropical freshwater fisheries : A dataset of occurrence and abundance of freshwater fishes in the Neotropics

The Neotropical region hosts 4225 freshwater fish species, ranking first among the world's most diverse regions for freshwater fishes. Our NEOTROPICAL FRESHWATER FISHES data set is the first to produce a large-scale Neotropical freshwater fish inventory, covering the entire Neotropical region from Mexico and the Caribbean in the north to the southern limits in Argentina, Paraguay, Chile, and Uruguay. We compiled 185,787 distribution records, with unique georeferenced coordinates, for the 4225 species, represented by occurrence and abundance data. The number of species for the most numerous orders are as follows: Characiformes (1289), Siluriformes (1384), Cichliformes (354), Cyprinodontiformes (245), and Gymnotiformes (135). The most recorded species was the characid Astyanax fasciatus (4696 records). We registered 116,802 distribution records for native species, compared to 1802 distribution records for nonnative species. The main aim of the NEOTROPICAL FRESHWATER FISHES data set was to make these occurrence and abundance data accessible for international researchers to develop ecological and macroecological studies, from local to regional scales, with focal fish species, families, or orders. We anticipate that the NEOTROPICAL FRESHWATER FISHES data set will be valuable for studies on a wide range of ecological processes, such as trophic cascades, fishery pressure, the effects of habitat loss and fragmentation, and the impacts of species invasion and climate change. There are no copyright restrictions on the data, and please cite this data paper when using the data in publications