Modified Debye-Huckel Electron Shielding and Penetration Factor

Screened potential, modified by non standard electron cloud distributions responsible for the shielding effect on fusion of reacting nuclei in astrophysical plasmas, is derived. The case of clouds with depleted tails in space coordinates is discussed. The modified screened potential is obtained both from statistical mechanics arguments based on fluctuations of the inverse of the Debye-Huckel radius and from the solution of a Bernoulli equation used in generalized statistical mechanics. Plots and tables useful in evaluating penetration probability at any energy are provided.Comment: 9 pages, 3 figures, 3 table

Variation Between Individuals in Voluntary Intake and Herbage Intake of Grazing Dairy Cows

Herbage intake and milk yield of unsupplemented grazing dairy cows are highly variable between animals within a herd (Delaby et al., 2001). The objective of this experiment was to describe the relationship between the individual voluntary intake (VI) of dairy cows measured before turnout and their herbage intake at grazing, at two herbage allowances

Wannier functions analysis of the nonlinear Schr\"{o}dinger equation with a periodic potential

In the present Letter we use the Wannier function basis to construct lattice approximations of the nonlinear Schr\"{o}dinger equation with a periodic potential. We show that the nonlinear Schr\"{o}dinger equation with a periodic potential is equivalent to a vector lattice with long-range interactions. For the case-example of the cosine potential we study the validity of the so-called tight-binding approximation i.e., the approximation when nearest neighbor interactions are dominant. The results are relevant to Bose-Einstein condensate theory as well as to other physical systems like, for example, electromagnetic wave propagation in nonlinear photonic crystals.Comment: 5 pages, 1 figure, submitted to Phys. Rev.

Heating of gas inside radio sources to mildly relativistic temperatures via induced Compton scattering

Measured values of the brightness temperature of low-frequency synchrotron radiation emitted by powerful extragalactic sources reach 10^11--10^12 K. If some amount of nonrelativistic ionized gas is present within such sources, it should be heated as a result of induced Compton scattering of the radiation. If this heating is counteracted by cooling due to inverse Compton scattering of the same radio radiation, then the plasma can be heated up to mildly relativistic temperatures kT~10--100 keV. The stationary electron velocity distribution can be either relativistic Maxwellian or quasi-Maxwellian (with the high-velocity tail suppressed), depending on the efficiency of Coulomb collisions and other relaxation processes. We derive several easy-to-use approximate expressions for the induced Compton heating rate of mildly relativistic electrons in an isotropic radiation field, as well as for the stationary distribution function and temperature of electrons. We also give analytic expressions for the kernel of the integral kinetic equation (one as a function of the scattering angle and another for the case of an isotropic radiation field), which describes the redistribution of photons in frequency caused by induced Compton scattering in thermal plasma. These expressions can be used in the parameter range hnu<< kT<~ 0.1mc^2 (the formulae earlier published in Sazonov, Sunyaev, 2000 are less accurate).Comment: 22 pages, 7 figures, submitted to Astronomy Letter

Metabolomics methods for the synthetic biology of secondary metabolism

Many microbial secondary metabolites are of high biotechnological value for medicine, agriculture, and the food industry. Bacterial genome mining has revealed numerous novel secondary metabolite biosynthetic gene clusters, which encode the potential to synthesize a large diversity of compounds that have never been observed before. The stimulation or “awakening” of this cryptic microbial secondary metabolism has naturally attracted the attention of synthetic microbiologists, who exploit recent advances in DNA sequencing and synthesis to achieve unprecedented control over metabolic pathways. One of the indispensable tools in the synthetic biology toolbox is metabolomics, the global quantification of small biomolecules. This review illustrates the pivotal role of metabolomics for the synthetic microbiology of secondary metabolism, including its crucial role in novel compound discovery in microbes, the examination of side products of engineered metabolic pathways, as well as the identification of major bottlenecks for the overproduction of compounds of interest, especially in combination with metabolic modeling. We conclude by highlighting remaining challenges and recent technological advances that will drive metabolomics towards fulfilling its potential as a cornerstone technology of synthetic microbiology

Metabolite Profiling Uncovers Plasmid-Induced Cobalt Limitation under Methylotrophic Growth Conditions

BACKGROUND:The introduction and maintenance of plasmids in cells is often associated with a reduction of growth rate. The reason for this growth reduction is unclear in many cases. METHODOLOGY/PRINCIPAL FINDINGS:We observed a surprisingly large reduction in growth rate of about 50% of Methylobacterium extorquens AM1 during methylotrophic growth in the presence of a plasmid, pCM80 expressing the tetA gene, relative to the wild-type. A less pronounced growth delay during growth under non-methylotrophic growth conditions was observed; this suggested an inhibition of one-carbon metabolism rather than a general growth inhibition or metabolic burden. Metabolome analyses revealed an increase in pool sizes of ethylmalonyl-CoA and methylmalonyl-CoA of more than 6- and 35-fold, respectively, relative to wild type, suggesting a strongly reduced conversion of these central intermediates, which are essential for glyoxylate regeneration in this model methylotroph. Similar results were found for M. extorquens AM1 pCM160 which confers kanamycin resistance. These intermediates of the ethylmalonyl-CoA pathway have in common their conversion by coenzyme B(12)-dependent mutases, which have cobalt as a central ligand. The one-carbon metabolism-related growth delay was restored by providing higher cobalt concentrations, by heterologous expression of isocitrate lyase as an alternative path for glyoxylate regeneration, or by identification and overproduction of proteins involved in cobalt import. CONCLUSIONS/SIGNIFICANCE:This study demonstrates that the introduction of the plasmids leads to an apparent inhibition of the cobalt-dependent enzymes of the ethylmalonyl-CoA pathway. Possible explanations are presented and point to a limited cobalt concentration in the cell as a consequence of the antibiotic stress

Methylobacterium Genome Sequences: A Reference Blueprint to Investigate Microbial Metabolism of C1 Compounds from Natural and Industrial Sources

Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus Methylobacterium, the reference species Methylobacterium extorquens strain AM1 and the dichloromethane-degrading strain DM4, were compared. Methodology/Principal Findings The 6.88 Mb genome of strain AM1 comprises a 5.51 Mb chromosome, a 1.26 Mb megaplasmid and three plasmids, while the 6.12 Mb genome of strain DM4 features a 5.94 Mb chromosome and two plasmids. The chromosomes are highly syntenic and share a large majority of genes, while plasmids are mostly strain-specific, with the exception of a 130 kb region of the strain AM1 megaplasmid which is syntenic to a chromosomal region of strain DM4. Both genomes contain large sets of insertion elements, many of them strain-specific, suggesting an important potential for genomic plasticity. Most of the genomic determinants associated with methylotrophy are nearly identical, with two exceptions that illustrate the metabolic and genomic versatility of Methylobacterium. A 126 kb dichloromethane utilization (dcm) gene cluster is essential for the ability of strain DM4 to use DCM as the sole carbon and energy source for growth and is unique to strain DM4. The methylamine utilization (mau) gene cluster is only found in strain AM1, indicating that strain DM4 employs an alternative system for growth with methylamine. The dcm and mau clusters represent two of the chromosomal genomic islands (AM1: 28; DM4: 17) that were defined. The mau cluster is flanked by mobile elements, but the dcm cluster disrupts a gene annotated as chelatase and for which we propose the name “island integration determinant” (iid).Conclusion/Significance These two genome sequences provide a platform for intra- and interspecies genomic comparisons in the genus Methylobacterium, and for investigations of the adaptive mechanisms which allow bacterial lineages to acquire methylotrophic lifestyles.Organismic and Evolutionary Biolog