Observations of Hippopotamus H. amphibius in the Little Scarcies River of Sierra Leone and Arguments for their conservation based on roles they play in riverine grasslands and nutrient loading

A small population of Hippopotamus amphibius remains in Sierra Leone and conservation efforts may be needed to support the Hippos and their indirect benefits to fisheries and grassland production. The hippo is a known ecosystem engineer, and a potentially important contributor of nutrients to the river systems they inhabit supporting the food web and local fisheries. In this study I observed hippos in the Little Scarcies River and uplands of the Outamba-Kilimi National Park to estimate their numbers and ultimately their potential input of nutrients into the river. Hippos forage at night in grasslands neighboring rivers, removing up to 3.62 ha of annual production, but spend most of the day submerged in the river. The group of hippos observed in this study contained up to 12 individuals, estimated to be contributing approximately 44 kilograms of wet matter (feces) into the river each day, liberating approximately 8 kg of dissolved organic carbon, 1.14 kg of total nitrogen, and 0.14 kg (140 g) of total phosphate to the Little Scarcies River. Because of these valuable nutrient contributions, as well as other ecosystem services provided by their movements and interactions with other species, hippopotami should be closely monitored and protected. Park management includes a buffer zone that has been created to mitigate anthropogenic changes to hippo habitat and ensure survival of the population, and with proper enforcement could help hippo populations. The results of this study are presented in the context of the broader picture of hippo conservation in Sierra Leone

Policies and Practices in Support of Mathematics Learning in Global Contexts

This issue of Global Education Review focuses on policies and practices in support of mathematics learning in global contexts. The issue highlights relevant research findings, illuminates areas that are in need of additional research, and examines the implications of these findings on educational outcomes. The ten manuscripts in this issue focus on three primary topics: efforts to improve student achievement and factors that impact achievement; implications of assessment frameworks, scoring, and analyses; and methods to improve teacher preparation and teacher knowledge. The manuscripts are organized in this issue by these topics

Policies and Practices in Support of Mathematics Learning in Global Contexts

This issue of Global Education Review focuses on policies and practices in support of mathematics learning in global contexts. The issue highlights relevant research findings, illuminates areas that are in need of additional research, and examines the implications of these findings on educational outcomes. The ten manuscripts in this issue focus on three primary topics: efforts to improve student achievement and factors that impact achievement; implications of assessment frameworks, scoring, and analyses; and methods to improve teacher preparation and teacher knowledge. The manuscripts are organized in this issue by these topics

Increased Abundance of the Common Raven Within the Ranges of Greater and Gunnison Sage-grouse: Influence of Anthropogenic Subsidies and Fire

The common raven (Corvus corax; raven) is native to North America and has increased in abundance, especially throughout western North America, during the last century. Human subsidies have facilitated raven dispersal into less suitable habitats and enabled these populations to maintain higher annual survival and reproduction. Concomitantly, overabundant raven populations are impacting other native at-risk species such as the greater sage-grouse (Centrocercus urophasianus) and potentially the Gunnison sage-grouse (C. minimus). Using Breeding Bird Survey data from 1995–2014, we evaluated raven count data to quantitatively describe changes in abundance and expansion into sagebrush (Artemisia spp.) ecosystems, specifically sage-grouse habitat. We focused our analyses on the 7 sage-grouse management zones (MZs) delineated across 11 western U.S. states and 2 Canadian provinces. We assessed the effects of land cover and anthropogenic disturbance on instantaneous growth rate (r) or carrying capacity (K) of ravens. Abundance of ravens in western and southeastern MZs was greater than northeastern MZs within the greater sage-grouse range. While raven abundance was lower in MZ I and II (Alberta, Canada; Dakotas, Montana, and northwestern Colorado, USA; Saskatchewan, Canada; and Wyoming, USA), raven expansion and percent increase were equivalent or greater than all other MZs. High abundance in MZ VII indicated Gunnison sage-grouse have been exposed to increased raven populations for several decades. Areas with greater electric power transmission line density had higher r; higher K was positively related to proportion of urban land cover within 25 km and burned area within 3 km and negatively related to greater distance from landfills and proportion of forest land cover within 15 km. Ravens have capitalized on human subsidies to increase abundance and expand into sagebrush ecosystems that did not historically support high raven populations. As such, managers are now faced with a new dilemma of reducing populations of a native species to benefit other native sagebrush obligate species

A Novel Technique to Improve Capture Success of Common Ravens

Traditional trapping techniques for common ravens (Corvus corax; raven) require significant effort, often produce low capture rates, and cannot be used in some situations. We designed a 3-m noose pole to secure ravens from nocturnal roost locations while using a strobe spotlight to temporarily disorient them. We collected measures of trapping efficiency and contrasted them with padded leghold traps also used in the study. We effectively implemented our noose pole method in July and August of 2018, 2019, and 2020 in the Baker and Cow Lakes sage-grouse (Centrocercus urophasianus) Priority Areas of Conservation in eastern Oregon, USA, which yielded trapping efficiency of 0.48 trap-hours/raven (37 total captured ravens). Our trapping efficiency using leghold traps during the same summer months was 76.42 trap-hours/raven (3 total captured ravens). Our new trapping method constitutes an inexpensive and simple way to safely trap ravens at accessible communal roosts and merits further refinement to increase utility and capitalize on the vulnerability of ravens to capture at night

Expression of Normally Repressed Myosin Heavy Chain 7b in the Mammalian Heart Induces Dilated Cardiomyopathy

In mammals, muscle contraction is controlled by a family of 10 sarcomeric myosin motors. The expression of one of its members, MYH7b, is regulated by alternative splicing, and while the protein is restricted to specialized muscles such as extraocular muscles or muscle spindles, RNA that cannot encode protein is expressed in most skeletal muscles and in the heart. Remarkably, birds and snakes express MYH7b protein in both heart and skeletal muscles. This observation suggests that in the mammalian heart, the motor activity of MYH7b may only be needed during development since its expression is prevented in adult tissue, possibly because it could promote disease by unbalancing myocardial contractility. Methods and Results We have analyzed MYH7b null mice to determine the potential role of MYH7b during cardiac development and also generated transgenic mice with cardiac myocyte expression of MYH7b protein to measure its impact on cardiomyocyte function and contractility. We found that MYH7b null mice are born at expected Mendelian ratios and do not have a baseline cardiac phenotype as adults. In contrast, transgenic cardiac MYH7b protein expression induced early cardiac dilation in males with significantly increased left ventricular mass in both sexes. Cardiac dilation is progressive, leading to early cardiac dysfunction in males, but later dysfunction in females. Conclusions The data presented show that the expression of MYH7b protein in the mammalian heart has been inhibited during the evolution of mammals most likely to prevent the development of a severe cardiomyopathy that is sexually dimorphic.</p

Evaluating the potential for the environmentally sustainable control of foot and mouth disease in Sub-Saharan Africa

Strategies to control transboundary diseases have in the past generated unintended negative consequences for both the environment and local human populations. Integrating perspectives from across disciplines, including livestock, veterinary and conservation sectors, is necessary for identifying disease control strategies that optimise environmental goods and services at the wildlife-livestock interface. Prompted by the recent development of a global strategy for the control and elimination of foot-and-mouth disease (FMD), this paper seeks insight into the consequences of, and rational options for potential FMD control measures in relation to environmental, conservation and human poverty considerations in Africa. We suggest a more environmentally nuanced process of FMD control that safe-guards the integrity of wild populations and the ecosystem dynamics on which human livelihoods depend while simultaneously improving socio-economic conditions of rural people. In particular, we outline five major issues that need to be considered: 1) improved understanding of the different FMD viral strains and how they circulate between domestic and wildlife populations; 2) an appreciation for the economic value of wildlife for many African countries whose presence might preclude the country from ever achieving an FMD-free status; 3) exploring ways in which livestock production can be improved without compromising wildlife such as implementing commodity-based trading schemes; 4) introducing a participatory approach involving local farmers and the national veterinary services in the control of FMD; and 5) finally the possibility that transfrontier conservation might offer new hope of integrating decision-making at the wildlife-livestock interface

Gene content evolution in the arthropods

Arthropods comprise the largest and most diverse phylum on Earth and play vital roles in nearly every ecosystem. Their diversity stems in part from variations on a conserved body plan, resulting from and recorded in adaptive changes in the genome. Dissection of the genomic record of sequence change enables broad questions regarding genome evolution to be addressed, even across hyper-diverse taxa within arthropods. Using 76 whole genome sequences representing 21 orders spanning more than 500 million years of arthropod evolution, we document changes in gene and protein domain content and provide temporal and phylogenetic context for interpreting these innovations. We identify many novel gene families that arose early in the evolution of arthropods and during the diversification of insects into modern orders. We reveal unexpected variation in patterns of DNA methylation across arthropods and examples of gene family and protein domain evolution coincident with the appearance of notable phenotypic and physiological adaptations such as flight, metamorphosis, sociality, and chemoperception. These analyses demonstrate how large-scale comparative genomics can provide broad new insights into the genotype to phenotype map and generate testable hypotheses about the evolution of animal diversity

Tumour risks and genotype-phenotype correlations associated with germline variants in succinate dehydrogenase subunit genes SDHB, SDHC and SDHD.

BACKGROUND: Germline pathogenic variants in SDHB/SDHC/SDHD are the most frequent causes of inherited phaeochromocytomas/paragangliomas. Insufficient information regarding penetrance and phenotypic variability hinders optimum management of mutation carriers. We estimate penetrance for symptomatic tumours and elucidate genotype-phenotype correlations in a large cohort of SDHB/SDHC/SDHD mutation carriers. METHODS: A retrospective survey of 1832 individuals referred for genetic testing due to a personal or family history of phaeochromocytoma/paraganglioma. 876 patients (401 previously reported) had a germline mutation in SDHB/SDHC/SDHD (n=673/43/160). Tumour risks were correlated with in silico structural prediction analyses. RESULTS: Tumour risks analysis provided novel penetrance estimates and genotype-phenotype correlations. In addition to tumour type susceptibility differences for individual genes, we confirmed that the SDHD:p.Pro81Leu mutation has a distinct phenotype and identified increased age-related tumour risks with highly destabilising SDHB missense mutations. By Kaplan-Meier analysis, the penetrance (cumulative risk of clinically apparent tumours) in SDHB and (paternally inherited) SDHD mutation-positive non-probands (n=371/67 with detailed clinical information) by age 60 years was 21.8% (95% CI 15.2% to 27.9%) and 43.2% (95% CI 25.4% to 56.7%), respectively. Risk of malignant disease at age 60 years in non-proband SDHB mutation carriers was 4.2%(95% CI 1.1% to 7.2%). With retrospective cohort analysis to adjust for ascertainment, cumulative tumour risks for SDHB mutation carriers at ages 60 years and 80 years were 23.9% (95% CI 20.9% to 27.4%) and 30.6% (95% CI 26.8% to 34.7%). CONCLUSIONS: Overall risks of clinically apparent tumours for SDHB mutation carriers are substantially lower than initially estimated and will improve counselling of affected families. Specific genotype-tumour risk associations provides a basis for novel investigative strategies into succinate dehydrogenase-related mechanisms of tumourigenesis and the development of personalised management for SDHB/SDHC/SDHD mutation carriers

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum