Fecal Coliform Transport through Intact Soil Blocks Amended with Poultry Manure

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and fecal coliforms through the soil blocks was not uniform. Fecal coliforms appeared where most drainage flowed. Drainage water from each soil block consistently exceeded 200 000 fecal coliforms per 100 mL and was as great as 30 million fecal coliforms per 100 mL of leachate collected. Fecal coliforms leached as a pulse, but the breakthrough of fecal coliforms through tilled blocks was delayed with respect to the breakthrough of fecal coliforms through sod-covered blocks. Rainfall on a well-structured soil will cause the preferential movement of fecal bacteria, even with unsaturated flow conditions, and could contribute to fecal coliform concentrations in shallow groundwater that exceed standards for domestic discharge and primary contact water in Kentucky (200 fecal coliforms/100 mL)

Tillage Slows Fecal Bacteria Infiltration through Soil

Bacterial pathogens can degrade ground water quality by infiltrating and eroding from land treated with poultry wastes. The potential for ground water contamination (as well as associated health risks and cost of water treatment) greatly depends on the depth of soil to the water table or bedrock and soil structure. Pathogens must move through the soil profile to contaminate ground water (although sinkholes can provide a direct channel from the soil surface to the water table in karst areas). Deep soils have less potential for contamination than shallow soils. Structureless soils retain fecal bacteria better than well structured soils. Research at UK indicates that surface-applied fecal bacteria, and other contaminants, travel rapidly toward ground water through soil pores in well structured, intact soil. Tillage disrupts pores and channels in the tilled layer, and increases water and bacteria contact with soil. To improve our understanding of bacterial movement, and of the potential for ground water contamination, we decided to examine whether tillage affected fecal coliform transport through intact soil amended with poultry wastes. We used poultry wastes because their disposal is an increasingly important waste management issue in western Kentucky

Fecal Coliform Transport through Intact Soil Blocks Amended with Poultry Manure

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and fecal coliforms through the soil blocks was not uniform. Fecal coliforms appeared where most drainage flowed. Drainage water from each soil block consistently exceeded 200 000 fecal coliforms per 100 mL and was as great as 30 million fecal coliforms per 100 mL of leachate collected. Fecal coliforms leached as a pulse, but the breakthrough of fecal coliforms through tilled blocks was delayed with respect to the breakthrough of fecal coliforms through sod-covered blocks. Rainfall on a well-structured soil will cause the preferential movement of fecal bacteria, even with unsaturated flow conditions, and could contribute to fecal coliform concentrations in shallow groundwater that exceed standards for domestic discharge and primary contact water in Kentucky (200 fecal coliforms/100 mL)

Solute Transport as Related to Soil Structure in Unsaturated Intact Soil Blocks

Concern about soil and groundwater pollution has resulted in numerous studies focused on solute transport. The objectives of our study were to investigate the effect of soil type and land-use management on solute movement. Transport of water and Cl− were measured through intact blocks of Maury (fine, mixed, semiactive, mesic Typic Paleudalf) and Cecil (fine, kaolinitic, thermic Typic Kanhapludult) soils, under steady-state, unsaturated flow conditions. Three replicate blocks for the Maury soil and two replicate blocks for the Cecil soil were studied per land-use treatment. The land-use treatments were conventional-till corn (Zea mays L.) production and long-term grass pasture. Individual blocks were instrumented with time domain reflectometry (TDR) probes at the 5-, 15-, and 25-cm depths. The effluent Cl− and TDR breakthrough curves were fitted using the convection dispersion equation (CDE); the estimated parameters were pore water velocity (v), dispersion coefficient (D), and, for the TDR breakthrough curves, maximum bulk electrical conductivity (BECmax). The CDE fitted the data very well, with model R 2 values ranging from 0.971 to 0.999. Volumetric water content (θ), total porosity, the soil water retention curve, and saturated hydraulic conductivity were determined on the same blocks. Volumetric water content increased (R2 = 0.25) as the slope of the water retention curve decreased. Increasing θ resulted in decreasing v (R2 =0.20) and thus, because of the linear relationship between D and v(R2 = 0.26), decreasing D Structural controls on solute dispersion in this study were mainly indirect, and related to variations in water content produced by differences in pore-size distribution

Phase Transition in Liquid Drop Fragmentation

A liquid droplet is fragmented by a sudden pressurized-gas blow, and the resulting droplets, adhered to the window of a flatbed scanner, are counted and sized by computerized means. The use of a scanner plus image recognition software enables us to automatically count and size up to tens of thousands of tiny droplets with a smallest detectable volume of approximately 0.02 nl. Upon varying the gas pressure, a critical value is found where the size-distribution becomes a pure power-law, a fact that is indicative of a phase transition. Away from this transition, the resulting size distributions are well described by Fisher's model at coexistence. It is found that the sign of the surface correction term changes sign, and the apparent power-law exponent tau has a steep minimum, at criticality, as previously reported in Nuclear Multifragmentation studies [1,2]. We argue that the observed transition is not percolative, and introduce the concept of dominance in order to characterize it. The dominance probability is found to go to zero sharply at the transition. Simple arguments suggest that the correlation length exponent is nu=1/2. The sizes of the largest and average fragments, on the other hand, do not go to zero but behave in a way that appears to be consistent with recent predictions of Ashurst and Holian [3,4].Comment: 10 pages, 11 figures. LaTeX (revtex4) with psfig/epsfi

Multiscale Soil Investigations: Physical Concepts And Mathematical Techniques

Soil variability has often been considered to be composed of “functional” (explained) variations plus random fl uctuations or noise. However, the distinction between these two components is scale dependent because increasing the scale of observation almost always reveals structure in the noise (Burrough, 1983). Soils can be seen as the result of spatial variation operating over several scales, indicating that factors infl uencing spatial variability differ with scale. Th is observation points to variability as a key soil attribute that should be studied

Deletion of Cryptococcus neoformans AIF Ortholog Promotes Chromosome Aneuploidy and Fluconazole-Resistance in a Metacaspase-Independent Manner

Apoptosis is a form of programmed cell death critical for development and homeostasis in multicellular organisms. Apoptosis-like cell death (ALCD) has been described in several fungi, including the opportunistic human pathogen Cryptococcus neoformans. In addition, capsular polysaccharides of C. neoformans are known to induce apoptosis in host immune cells, thereby contributing to its virulence. Our goals were to characterize the apoptotic signaling cascade in C. neoformans as well as its unique features compared to the host machinery to exploit the endogenous fungal apoptotic pathways as a novel antifungal strategy in the future. The dissection of apoptotic pathways revealed that apoptosis-inducing factor (Aif1) and metacaspases (Mca1 and Mca2) are independently required for ALCD in C. neoformans. We show that the apoptotic pathways are required for cell fusion and sporulation during mating, indicating that apoptosis may occur during sexual development. Previous studies showed that antifungal drugs induce ALCD in fungi and that C. neoformans adapts to high concentrations of the antifungal fluconazole (FLC) by acquisition of aneuploidy, especially duplication of chromosome 1 (Chr1). Disruption of aif1, but not the metacaspases, stimulates the emergence of aneuploid subpopulations with Chr1 disomy that are resistant to fluconazole (FLCR) in vitro and in vivo. FLCR isolates in the aif1 background are stable in the absence of the drug, while those in the wild-type background readily revert to FLC sensitivity. We propose that apoptosis orchestrated by Aif1 might eliminate aneuploid cells from the population and defects in this pathway contribute to the selection of aneuploid FLCR subpopulations during treatment. Aneuploid clinical isolates with disomies for chromosomes other than Chr1 exhibit reduced AIF1 expression, suggesting that inactivation of Aif1 might be a novel aneuploidy-tolerating mechanism in fungi that facilitates the selection of antifungal drug resistance

Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium.

BACKGROUND: Invasive fungal diseases (IFDs) remain important causes of morbidity and mortality. The consensus definitions of the Infectious Diseases Group of the European Organization for Research and Treatment of Cancer and the Mycoses Study Group have been of immense value to researchers who conduct clinical trials of antifungals, assess diagnostic tests, and undertake epidemiologic studies. However, their utility has not extended beyond patients with cancer or recipients of stem cell or solid organ transplants. With newer diagnostic techniques available, it was clear that an update of these definitions was essential. METHODS: To achieve this, 10 working groups looked closely at imaging, laboratory diagnosis, and special populations at risk of IFD. A final version of the manuscript was agreed upon after the groups' findings were presented at a scientific symposium and after a 3-month period for public comment. There were several rounds of discussion before a final version of the manuscript was approved. RESULTS: There is no change in the classifications of "proven," "probable," and "possible" IFD, although the definition of "probable" has been expanded and the scope of the category "possible" has been diminished. The category of proven IFD can apply to any patient, regardless of whether the patient is immunocompromised. The probable and possible categories are proposed for immunocompromised patients only, except for endemic mycoses. CONCLUSIONS: These updated definitions of IFDs should prove applicable in clinical, diagnostic, and epidemiologic research of a broader range of patients at high-risk

A Prospective Longitudinal Study of the Clinical Outcomes from Cryptococcal Meningitis following Treatment Induction with 800 mg Oral Fluconazole in Blantyre, Malawi

Introduction: Cryptococcal meningitis is the most common neurological infection in HIV infected patients in Sub Saharan Africa, where gold standard treatment with intravenous amphotericin B and 5 flucytosine is often unavailable or difficult to administer. Fluconazole monotherapy is frequently recommended in national guidelines but is a fungistatic drug compromised by uncertainty over optimal dosing and a paucity of clinical end-point outcome data. Methods: From July 2010 until March 2011, HIV infected adults with a first episode of cryptococcal meningitis were recruited at Queen Elizabeth Central Hospital, Blantyre, Malawi. Patients were treated with oral fluconazole monotherapy 800 mg daily, as per national guidelines. ART was started at 4 weeks. Outcomes and factors associated with treatment failure were assessed 4, 10 and 52 weeks after fluconazole initiation. Results: Sixty patients were recruited. 26/60 (43%) died by 4 weeks. 35/60 (58.0%) and 43/56 (77%) died or failed treatment by 10 or 52 weeks respectively. Reduced consciousness (Glasgow Coma Score ,14 of 15), moderate/severe neurological disability (modified Rankin Score .3 of 5) and confusion (Abbreviated Mental Test Score ,8 of 10) were all common at baseline and associated with death or treatment failure. ART prior to recruitment was not associated with better outcomes. Conclusions: Mortality and treatment failure from cryptococcal meningitis following initiation of treatment with 800 mg oral fluconazole is unacceptably high. To improve outcomes, there is an urgent need for better therapeutic strategies and point-of-care diagnostics, allowing earlier diagnosis before development of neurological deficit