Potential use of endophytic bacteria to promote the plant growth of micropropagated banana cultivar Prata Anã

Endophytic bacteria isolated from shoot tip cultures of banana ‘Tropical’ and ‘Galil 18’ were identified at the genera and species levels by means of the profile of fatty acids test through gas chromatography and the potential of plant growth promotion of micropropagated ‘Prata Anã’ banana plantlets was evaluated. A completely randomized experimental design in a factorial scheme 2 × 9, with two immersion periods (30 and 60 min), eight isolates (endophytic bacteria) plus control (saline solution; 0.85% sodium chloride) with seven replications was used. The plantlets were cultured under greenhouse conditions and the evaluations were performed at 24 (DAP1) and 48 (DAP2) days after planting. The isolates of endophytic bacteria affected significantly pseudostem height, number of leaves and pseudostem diameter. Five isolates: 03, 05, 06, 07 and 08 showed percentages of increment index over 20% for pseudostem height. Among them, the isolate 07, Klebsiella pneumoniae pneumoniae showed the highest increment indices: 27.48, 13.68 and 13.55% for pseudostem height, number of leaves and pseudostem diameter, respectively. The results show the potential use of K. pneumoniae pneumoniae (Isolate 07) for growth promotion in micropropagated ‘Prata Anã’ banana plantlets.Keywords: Musa sp., Klebsiella pneumoniae pneumoniae, plant growth promoting bacteriaAfrican Journal of Biotechnology Vol. 12(31), pp. 4915-491

Clinical and Laboratory Profile of Dogs Seroreactive to Ehrlichiosis Treated at the Veterinary Medical Teaching Hospital in Niterói, State of Rio de Janeiro, Brazil

Background: Ehrlichiosis is a tick-borne disease highly prevalent in Brazil, and is relevant in canine clinical practice due to its high morbidity and mortality. Its clinical signs are nonspecific and its phases are acute, lasting 2 to 4 weeks; subclinical, i.e., asymptomatic; and chronic, resembling an autoimmune disease. The purpose of this study was to identify the occurrence of reactivity to Ehrlichia canis of bitches treated at the Veterinary Medical Teaching Hospital of the Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil, based on serological examination by iELISA, and to compare the hematological, biochemical, urinary protein-creatinine and urinary density profiles of reactive and non-reactive animals. Materials, Methods & Results: This study involved solely bitches, regardless of breed, starting at 1 year of age. One hundred and thirty bitches, 1 to 16 year-old (mean age 7.02 ± 4.00), weighing 1.5 to 50 kg (mean weight 12.12 ± 10.65) were subjected to clinical examination and abdominal ultrasound. Complete blood count, biochemical measurements, urinalysis and serology for E. canis were also performed. The serum was used in the iELISA to identify immunoglobulin G (IgG), using a canine Ehrlichia Imunotest® diagnostic kit (Imunodot®, Jaboticabal, SP, Brazil) according to the manufacturer’s instructions. Sixty animals (46.20%) were reactive to E. canis. According to their owners, only 5 (8.3%) of the 60 seroreactive animals had a history of tick-borne disease. The most common profile was that of mixed breed animals living with their owners, older than 7 years, who had not been treated preventatively with specific drugs against ectoparasites. Laboratory tests showed significant differences between groups in terms of total protein (TP), and calcium and urinary protein-creatinine ratio (UPC). TP and UPC were elevated in the non-reactive group, while the only significant change in the reactive group was mild hypocalcemia. In this study, 30% (18/60) of the bitches were seroreactive to E. canis and had hypocalcemia. Of these, 50% (9/18) had a UPC above 0.5. Furthermore, 66.7% (12/18) of this group with hypocalcemia also showed urine density (UD) of less than 1024. Among these 18 bitches, 5 had both alterations, i.e., UPC > 0.5 and UD < 1024.Discussion: In this study, a high prevalence of bitches seroreactive to Ehrlichia canis was observed, despite the absence of clinical and/or laboratory signs indicative of the disease. In the investigation of IgG class antibodies, it is not possible to determine the exact time of infection, and titers may remain high for a period of more than 11 months, even after treatment and elimination of the bacterium. The fact that most seroreactive bitches showed no symptoms compatible with the disease either before or during the study suggests that they were in the subclinical phase of ehrlichiosis. The main reason for calcium metabolism disorders is a phosphorus imbalance, a condition that occurs in kidney diseases. Isosthenuria reflects the kidney’s inability to concentrate urine. This finding may be one of the first clinical manifestations of chronic kidney disease (CKD), especially in dogs. On the other hand, the UPC ratio may increase with the progression of CKD. The presence of hypocalcemia, isosthenuria and increased UPC associated with seroreactivity suggests that infection by E. canis may be associated with the onset of CKD. Veterinarians should keep in mind the complexity of the pathophysiology of ehrlichiosis to ensure the disease is not underdiagnosed in any of its phases, thereby ensuring the correct treatment is provided. Such awareness is expected to reduce the chronicity of the disease and underlying sequelae among dogs.Keywords: Ehrlichia canis, serology, tick, clinic, renal.Descritores: Ehrlichia canis, sorologia, carrapato, clínica, renal.Título: Perfil clínico e laboratorial de cadelas sororeativas para erliquiose tratadas em um Hospital Veterinário Universitário em Niterói, Estado do Rio de Janeiro, Brasil

Biofilm - forming characteristics of vancomycin - resistant Enterococcus faecium vanA of the epidemic clonal complex 17 isolated in a university hospital of southern Brazil: Características dos biofilmes formados por Enterococcus faecium resistente à vancomicina vanA do complexo clonal epidêmico 17 isolado em um hospital universitário do sul do Brasil

Vancomycin-resistant Enterococcus faecium (VREfm) is an important etiologic agent of healthcare-associated infections worldwide. The present study aimed to characterize the vanA-carrying VREfm isolates according to their genetic relatedness, multilocus sequence typing (MLST), biofilm-forming characteristics, and the occurrence of putative genes involved in adhesion, biofilm formation, and pili assembly. High genetic diversity among the ten vanA VREfm isolated from inpatients was observed using the rep-PCR analysis. According to the MLST analyses, four different STs were detected among the vanA VREfm isolates, with ST 412 being the most prevalent. Most of the STs detected in this study belong to Clonal Complex 17 (CC 17), a high-risk clone adapted to the hospital environment. All VREfm&nbsp;were capable of forming biofilm on a polystyrene surface. However, significant differences in biofilm biomass were observed among the different isolates, including those with the same ST. Scanning electron microscopy analyses of the 24-h biofilms revealed the presence of sessile cells surrounded by an extracellular polymeric substance. All VREfm harbored the atlA gene, and nine were positive for the ecbA gene. The acm, scm, and ebpC genes were detected in six isolates. Finally, the fms21/pilA gene was detected in seven isolates

Screening of six medicinal plant species for antileishmanial activity

This study is aimed to investigate the in vitro antileishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 µg mL–1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 µg mL–1 for L. amazonensis and > 200 µg mL–1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 µg mL–1 and hemolytic activity from 0 to 46.1 %

Análise ecológica dos acidentes e da violência letal em Vitória, ES

OBJECTIVE: To analyze the socioeconomic background and its relationship with spatial distribution of mortality due to violence. METHODS: Ecological study conducted to explore the space distribution of mortality due to violence in the city of Vitória, Southeastern Brazil, between 2000 and 2003, based on population and socioeconomic information. Mortality data were correlated with information on victim's place of residence, type of violence, gender, and skin color. Data were analyzed using space average, odds ratio, and cluster analysis. RESULTS: There were reported 828 deaths due to violence during the study period, accounting for 17% of all deaths in the city. Of these, 72% were homicides, 21.8% traffic accidents, and 6% suicides. Violence victims were mostly young black males, living in poorer areas of the city. In contrast, as for suicide and traffic accidents, the victims were older white females living in the wealthiest area of the city. CONCLUSIONS: The study showed that violence is a phenomenon occurring in all socioeconomic levels but black people at the lowest level are more likely to die from homicides while white well-off people are more likely to die from suicide and traffic accidents.OBJETIVO: Analizar el contexto socioeconómico y su relación con la incidencia espacial de la mortalidad debido a la violencia. MÉTODOS: Fue realizado estudio del tipo ecológico en el municipio de Vitória (Sureste de Brasil), de 2000 a 2003, sobre la distribución espacial de la mortalidad por accidentes y violencia, con base en las informaciones poblacionales y socioeconómicas. Los datos sobre mortalidad fueron relacionados con informaciones como lugar de residencia de la víctima, tipo de ocurrencia, sexo y raza/color. El análisis de las informaciones utilizó el promedio espacial, odds ratio y análisis de cluster. RESULTADOS: Ocurrieron 828 óbitos por violencia en el período estudiado, representando 17% del total de óbitos del municipio. De estos, 72% eran homicidios, 21,8% accidentes de transporte y 6% suicidios. El patrón de las víctimas de los homicidios fue ser joven, negro, del sexo masculino y residente en regiones más pobres de la ciudad. Suicidios y accidentes de transporte afectaron víctimas más viejas, blancas, del sexo femenino y residentes en el área más rica de la ciudad. CONCLUSIONES: Los resultados muestran que la violencia es un fenómeno que afecta todas las clases sociales, con destaque para las personas de raza negra y bajo nivel socioeconómico que tienen mayor oportunidad de muerte por homicidio; y blancos de nivel socioeconómico más elevado, suicidios y accidentes de transporte predominan.OBJETIVO: Analisar o contexto socioeconômico e sua relação com a incidência espacial da mortalidade devido à violência. MÉTODOS: Foi realizado estudo do tipo ecológico no município de Vitória, ES, de 2000 a 2003, sobre a distribuição espacial da mortalidade por acidentes e violência, com base nas informações populacionais e socioeconômicas. Os dados sobre mortalidade foram relacionados a informações como local de residência da vítima, tipo de ocorrência, sexo e raça/cor. A análise das informações utilizou a média espacial, odds ratio e análise de cluster. RESULTADOS: Ocorreram 828 óbitos por violência no período estudado, representando 17% do total de óbitos do município. Destes, 72% eram homicídios, 21,8% acidentes de transporte e 6% suicídios. O padrão das vítimas dos homicídios foi ser jovem, negro, do sexo masculino e residente em regiões mais pobres da cidade. Suicídios e acidentes de transporte acometeram vítimas mais velhas, brancas, do sexo feminino e residentes na área mais rica da cidade. CONCLUSÕES: O resultados mostram que a violência é um fenômeno que atinge todas as classes sociais, com destaque para as pessoas da raça negra e baixo nível socieconômico que têm maior chance de morte por homicídio; e brancos de nível socioeconômico mais elevado, suicídios e acidentes de transporte se sobressaem

Carbamazepine inhibits angiotensin I-converting enzyme, linking it to the pathogenesis of temporal lobe epilepsy

We find that a common mutation that increases angiotensin I-converting enzyme activity occurs with higher frequency in male patients suffering from refractory temporal lobe epilepsy. However, in their brains, the activity of the enzyme is downregulated. As an explanation, we surprisingly find that carbamazepine, commonly used to treat epilepsy, is an inhibitor of the enzyme, thus providing a direct link between epilepsy and the renin-angiotensin and kallikrein-kinin systems. Translational Psychiatry (2012) 2, e93; doi:10.1038/tp.2012.21; published online 13 March 2012INNTConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Biophys, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023032 São Paulo, BrazilUniv São Paulo, Sch Arts Sci & Humanities, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Sci & Technol, BR-04023032 São Paulo, BrazilNove de Julho Univ UNINOVE, Dept Rehabil Sci, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Sci & Technol, BR-04023032 São Paulo, BrazilWeb of Scienc

Estratégias Emergentes no Manejo do Acidente Vascular Cerebral - Perspectivas e Desafios

This paper presents a systematic review on approaches to the management of acute ischemic stroke (AIS) in the emergency setting, highlighting therapeutic interventions such as thrombolytic therapy and mechanical thrombectomy. The analysis of the reviewed studies reveals significant advances in the treatment of acute stroke, including the expansion of therapeutic windows and the growing role of endovascular intervention. Despite progress, challenges persist in early diagnosis, effective patient referral, and availability of specialized resources. Continuous research and interdisciplinary collaboration are crucial to advancing understanding and treatment of stroke in the emergency setting, aiming to improve clinical outcomes and patients' quality of life.Este trabalho apresenta uma revisão sistemática sobre as abordagens para o manejo do acidente vascular cerebral (AVC) na emergência, destacando intervenções terapêuticas como terapia trombolítica e trombectomia mecânica. A análise dos estudos revisados revela avanços significativos no tratamento do AVC agudo, incluindo a ampliação das janelas terapêuticas e o papel crescente da intervenção endovascular. Apesar dos progressos, desafios persistem no diagnóstico precoce, encaminhamento eficaz de pacientes e disponibilidade de recursos especializados. A pesquisa contínua e a colaboração interdisciplinar são fundamentais para avançar na compreensão e no tratamento do AVC na emergência, visando melhorar os desfechos clínicos e a qualidade de vida dos pacientes

estudos artísticos

O tropicalismo arranca em 1967, através do corpo: a música de Caetano Veloso e Gilberto Gil, os vestíveis de Hélio Oiticica, as propostas teatrais de José Celso Martinez Corrêa e os cenários de Hélio Eichbauer. Hoje as coisas são um pouco mais complexas. Em tempo de redes sociais, os aspirantes ao poder fazem uso da sua imediatez para suscitarem reações epidérmicas, superficiais, populistas e de grande instantaneidade. A boçalidade triunfa nas caixas de comentários, e com mais alguns perfis falsificados podem manipular-se plebiscitos, movimentos secessionistas, ou, e também censurar-se exposições de arte. Nesta variação do fascismo, a epiderme eletrificada das redes sociais estrutura-se como uma poderosa arena onde se aparenta uma falsa democracia. Talvez a arte continue a ser um reduto para reflexão, mas vemos que a censura se manifesta hoje de modo talvez mais eficaz, silenciando artistas e professores, através da pressão mediatizada, da emoção do momento. Para isto é necessária a atenção consciente da arte, dos artistas, e também dos arte-educadores: enfrenta-se uma massa cada vez mais informe, alienada e despojada de reflexão para além do imediato.info:eu-repo/semantics/publishedVersio

Paciente pediátrico portador de transtorno espectro autista em um ambulatório: relato de experiência / Pediatric patient with autistic spectrum disorder in an outpatient clinic: experience report

O transtorno do espectro autista (TEA) é um grupo de distúrbios do desenvolvimento neurológico de início precoce, caracterizado por comprometimento das habilidades sociais e de comunicação, além de comportamentos estereotipados. Este trabalho teve como objetivo relatar a experiência vivenciadas durante o período das aulas práticas em um ambulatório pediátrico, pontuando a assistência de enfermagem para um paciente pediátrico com Transtorno do Espectro Autista (TEA), e os cuidados voltados para as suas enfermidades. Trata-se de um estudo descritivo do tipo relato de experiência, realizado por acadêmicas de enfermagem da Universidade da Amazônia - UNAMA, durante as aulas práticas em um ambulatório infantil em Belém – PA, concluímos que a importância do enfermeiro sobre orientar os pais, e direciona-los a consultar o filho com outros profissionais da equipe multiprofissional de saúde, e para melhorar a qualidade de vida da criança.