Transfer of antibiotic resistance in Staphylococcus aureus

Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera. Transfer is mediated by a diverse complement of mobile genetic elements and occurs primarily by conjugation or bacteriophage transduction, with the latter traditionally being perceived as the primary route. Recent work on conjugation and transduction suggests that transfer by these mechanisms may be more extensive than previously thought, in terms of the range of plasmids that can be transferred by conjugation and the efficiency with which transduction occurs. Here, we review the main routes of antibiotic resistance gene transfer in S. aureus in the context of its biology as a human commensal and a life-threatening pathogen

A Machine Learning Approach to Prediction of the Compressive Strength of Segregated Lightweight Aggregate Concretes Using Ultrasonic Pulse Velocity

Lightweight aggregate concrete (LWAC) is an increasingly important material for modern construction. However, although it has several advantages compared with conventional concrete, it is susceptible to segregation due to the low density of the incorporated aggregate. The phenomenon of segregation can adversely affect the mechanical properties of LWAC, reducing its compressive strength and its durability. In this work, several machine learning techniques are used to study the influence of the segregation of LWAC on its compressive strength, including the K-nearest neighbours (KNN) algorithm, regression tree-based algorithms such as random forest (RF) and gradient boosting regressors (GBRs), artificial neural networks (ANNs) and support vector regression (SVR). In addition, a weighted average ensemble (WAE) method is proposed that combines RF, SVR and extreme GBR (or XGBoost). A dataset that was recently used for predicting the compressive strength of LWAC is employed in this experimental study. Two different types of lightweight aggregate (LWA), including expanded clay as a coarse aggregate and natural fine limestone aggregate, were mixed to produce LWAC. To quantify the segregation in LWAC, the ultrasonic pulse velocity method was adopted. Numerical experiments were carried out to analyse the behaviour of the obtained models, and a performance improvement was shown compared with the machine learning models reported in previous works. The best performance was obtained with GBR, XGBoost and the proposed weighted ensemble method. In addition, a good choice of weights in the WAE method allowed our approach to outperform all of the other models.This research was funded by MCIN/AEI/10.13039/501100011033, grant PID2021-123627OB-C55 and by “ERDF A way of making Europe”

Construcción industrializada de edificios

In this article several examples about industrial construction of buildings are analysed. One of these example, considered to be the best in complete prefabricated construction of buildings. refers to the housings built in Japan. Other cases analysed are buildings constructed in Spain with different prefabricated levels.En este artículo se analizan algunos ejemplos sobre la prefabricación de edificios. Desde el caso que se podría considerar como ideal, la prefabricación integral de viviendas en Japón hasta el análisis de algunas obras, más o menos industrializadas, realizadas en España

Product Line-based customization of e-Government documents

International audienceContent personalization has been one of the major trends in recent Document Engineering Research. The "one docum ent for n users" paradigm is being replaced by the "one user, one document" model, where the content to be delivered to a particular user is generated by some means. This is a very promising approach for e-Government, where personalized government services, including document generation, are more and more required by users. In this paper, we introduce a method to the generation of personalized documents called Document Product Lines (DPL). DPL allows generating content in domains with high variability and with high levels of reuse. We describe the basic principles underlying DPL and show its application to the e-Government field using the personalized tax statement as case study

Deciphering the molecular mechanism underpinning phage arbitrium communication systems

Bacillus phages use a communication system, termed “arbitrium,” to coordinate lysis-lysogeny decisions. Arbitrium communication is mediated by the production and secretion of a hexapeptide (AimP) during lytic cycle. Once internalized, AimP reduces the expression of the negative regulator of lysogeny, AimX, by binding to the transcription factor, AimR, promoting lysogeny. We have elucidated the crystal structures of AimR from the Bacillus subtilis SPbeta phage in its apo form, bound to its DNA operator and in complex with AimP. AimR presents intrinsic plasticity, sharing structural features with the RRNPP quorum-sensing family. Remarkably, AimR binds to an unusual operator with a long spacer that interacts nonspecifically with the receptor TPR domain, while the HTH domain canonically recognizes two inverted repeats. AimP stabilizes a compact conformation of AimR that approximates the DNA-recognition helices, preventing AimR binding to the aimX promoter region. Our results establish the molecular basis of the arbitrium communication system

A framework for variable content document generation with multiple actors

“NOTICE: this is the author’s version of a work that was accepted for publication in Information and Software Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Information and Software Technology, Volume 56, Issue 9, September 2014, Pages 1101–1121 DOI10.1016/j.infsof.2013.12.006Context - Advances in customization have highlighted the need for tools supporting variable content document management and generation in many domains. Current tools allow the generation of highly customized documents that are variable in both content and layout. However, most frameworks are technology-oriented, and their use requires advanced skills in implementation-related tools, which means their use by end users (i.e. document designers) is severely limited. Objective - Starting from past and current trends for customized document authoring, our goal is to provide a document generation alternative in which variants are specified at a high level of abstraction and content reuse can be maximized in high variability scenarios. Method Based on our experience in Document Engineering, we identified areas in the variable content document management and generation field open to further improvement. We first classified the primary sources of variability in document composition processes and then developed a methodology, which we called DPL based on Software Product Lines principles to support document generation in high variability scenarios. Results - In order to validate the applicability of our methodology we implemented a tool DPLfw to carry out DPL processes. After using this in different scenarios, we compared our proposal with other state-of-the-art tools for variable content document management and generation. Conclusion - The DPLfw showed a good capacity for the automatic generation of variable content documents equal to or in some cases surpassing other currently available approaches. To the best of our knowledge, DPLfw is the only framework that combines variable content and document workflow facilities, easing the generation of variable content documents in which multiple actors play different roles.This work has been partially funded by the Spanish Ministerio de Economia y Competitividad under Grant TIPEx (TIN2010-19859-C03-03).Gómez Llana, A.; Penadés Gramage, MC.; Canos Cerda, JH.; Borges, MR.; Llavador Campos, M. (2014). A framework for variable content document generation with multiple actors. Information and Software Technology. 56(9):1101-1121. https://doi.org/10.1016/j.infsof.2013.12.006S1101112156

Life cycle assessment of earth-retaining walls: An environmental comparison

[EN] Earth-retaining walls are one of the most common structures in civil engineering, a discipline of the construction sector, which is known to produce one of the highest environmental impacts. Therefore, developing cleaner design and construction practices could contribute to a more sustainable future for our planet. To make a step towards this goal, this study comprises the life cycle assessment (LCA) of the four most common earth-retaining walls built between 1 to 6 m of height: cantilever walls, gravity walls, masonry walls and gabion walls to obtain the best solutions for the environment. To assess the environmental impacts caused throughout their whole life-cycle including the production, construction, use and end of life phases, we used the OpenLCA software, the ecoinvent 3.3 database and the ReCiPe (H) method. The associated uncertainties have been considered and the results are provided in both midpoint and endpoint approaches. Our findings show that gabion and masonry walls produce the lowest global impact. On the one hand, gabion walls cause less damage to human health but on the other hand, masonry walls cause less damage to the ecosystems. Furthermore, gravity walls produce similar impacts to gabion and masonry walls between 1 and 3 m of height as well as fewer impacts than cantilever walls for a height of 4 m. In conclusion, gabion and masonry walls are preferable to concrete walls for heights between 1 and 6 m and cantilever walls should be used over gravity walls for greater heights than 4.5 m.This research was funded by the Spanish Ministry of Economy and Competitiveness along with FEDER funding (Project BIA2017-85098-R).Pons, J.; Penadés-Plà, V.; Yepes, V.; Martí Albiñana, JV. (2018). Life cycle assessment of earth-retaining walls: An environmental comparison. Journal of Cleaner Production. 192:411-420. doi:10.1016/j.jclepro.2018.04.268S41142019

Building Urban Resilience: A Dynamic Process Composition Approach

Urban resilience (also referred to as city resilience) has become a strategic goal of city administrators. Given the diversity of threats and city contexts, managing urban resilience is a complex task that has been conceptualized as a process by the so-called urban resilience frameworks proposed during the last decade. But conceptualization is not enough: an urban resilience building process may last for months, even years, and needs to coordinate many different actors using different tools. Therefore, some type of tool support is required for process control. In this paper, we introduce a proposal for the operationalization of urban resilience processes based on the notion of process family. The notion of family allows to deal with the natural diversity of urban resilience, and its transformation into a process specification allows the enactment, monitoring and measuring of the process. We have applied our approach to the well-known Smart Mature Resilience framework