Les relations phylogénétiques au sein d'un système réticulé : cas particulier de Cytisus scoaprius L. (Genisteae, Fabaceae) et des espèces, hybrides et cultivars apparentés.

The " scoparius " group is composed of Cytisus scoparius (Genisteae, Fabaceae) and its related taxa. Taxonomic and morphometric studies enabled us to delimitate 10 species divided into three sections: Alburnoides, Spartopsis and Verzinum. Phylogenetic analyses based on chloroplastic markers trnD-T and trnS-G and nuclear markers ITS, LEGCYCIAa, LEGCYCIAb and LEGCYCIAc revealed that sections Alburnoides and Verzinum, both monophyletic if C. Striatus is included within section Alburnoides, are sister clades. Section Spartopsis, either paraphyletic or monophyletic depending on the position of C. Commutatus (sect. Corothamnus), is more distantly related. Four cultivated hybrids result from crossbreedings between species of the " scoparius " group. The comparison of polymorphic ITS sequences obtained from cloning confirmed both parents at the origin of three of those hybrids and one parent for the fourth hybrid of the group. The use of trnD-T sequences determined the maternal parent at the origin of three of the hybrids. The addition of hybrid taxa in the phylogenetic analyses showed that hybrids between closely related taxa form a clade with their maternal parent and do not modify the relationships between non-hybrid taxa. However, hybrids between distant taxa do not necessarily place with one of their parents and can cause the collapsing of some clades and changes in the topology. More than 150 cultivars are also included in the " scoparius " group, 40 of which were characterised. Molecular data (ISSR, trnD-T sequences and microsatellites) were used to confirm and complete the genealogical information on 28 of those cultivar whose origin is resumed in a 7-generation pedigree on broom cultivars

Test in a beam of large-area Micromegas chambers for sampling calorimetry

Application of Micromegas for sampling calorimetry puts specific constraints on the design and performance of this gaseous detector. In particular, uniform and linear response, low noise and stability against high ionisation density deposits are prerequisites to achieving good energy resolution. A Micromegas-based hadronic calorimeter was proposed for an application at a future linear collider experiment and three technologically advanced prototypes of 1$\times$1 m$^{2}$ were constructed. Their merits relative to the above-mentioned criteria are discussed on the basis of measurements performed at the CERN SPS test-beam facility

Structure of an active volcano associated with a resurgent block inferred from thermal mapping: The Yasur–Yenkahe volcanic complex (Vanuatu)

International audienceSubsurface thermal measurements provide a valuable tool to map hydrothermal-fluid release zones in active volcanic areas. On the Yasur–Yenkahe volcanic complex (Tanna Island, Vanuatu archipelago), fumaroles and hot springs abound, signs of upraising heat fluxes associated to a well-developed hydrothermal activity. Combination of high resolution mapping of ground thermal anomalies with geomorphological analysis allows the characterization of the structural relationships between the active Yasur volcano and the Yenkahe resurgent block.A complex system of heat release and hydrothermal fluid circulation below the Yasur–Yenkahe complex is evidenced. Circulation, though propagating vertically as a whole, is funneled by stratification. Thus, the main thermal fluid released is almost exclusively concentrated along structural limits that break the seals induced by the stratified nature of the ground. Three types of medium/high temperature anomalies have been evidenced: (1) broad hydrothermalized areas linked with planar stratification that favor lateral spreading, (2) linear segments that represent active faults, and (3) arcuate segments related to paleo-crater rims. The limit between the Yasur volcano and the Yenkahe resurgent block is characterized by an active fault system accommodating both the rapid uplift of the Yenkahe block and the overloading induced by the volcano weight. In such a setting, faults converge below the cone of Yasur, which acts as a focus for the faults. Evidence of such structures, sometimes hidden in the landscape but detected by thermal measurements, is critical for risk assessment of flank landslides

Subcellular distribution of carbonic anhydrase in Solanum tuberosum L. leaves

The intracellular compartmentation of carbonic anhydrase (CA; EC 4.2.1.1), an enzyme that catalyses the reversible hydration of CO2 to bicarbonate, has been investigated in potato (#Solanum tuberosum$ L.) leaves. Although enzyme activity was mainly located in chloroplasts (87% of total cellular activity), significant activity (13%) was also found in the cytosol. The corresponding CA isoforms were purified either from chloroplasts or crude leaf extracts, respectively. The cytosolic isoenzyme has a molecular mass of 255 000 and is composed of eight identical subunits with an estimated Mr of 30 000. The chloroplastic isoenzyme (Mr 220 000) is also an octamer composed of two different subunits with Mr estimated at 27 000 and 27 500, respectively. The N-terminal amino acid sequences of both chloroplastic CA subunits demonstrated that they were identical except that the Mr-27 000 subunit was three amino acids shorter than that of the Mr-27 500 subunit. Cytosolic and chloroplastic CA isoenzymes were found to be similarly inhibited by monovalent anions (Cl-, I-, N3- and NO3-) and by sulfonamides (ethoxyzolamide and acetozolamide). Both CA isoforms were found to be dependent on a reducing agent such as cysteine or dithiothreitol in order to retain the catalytic activity, but 2-mercaptoethanol was found to be a potent inhibitor. A polyclonal antibody directed against a synthetic peptide corresponding to the N-terminal amino acid sequence of the chloroplastic CA monomers also recognized the cytosolic CA isoform. This antibody was used for immunocytolocalization experiments which confirmed the intracellular compartmentation of CA : within chloroplasts, CA is restricted to the stroma and appears randomly distributed in the cytosol. (Résumé d'auteur

Connectivity forests for homological analysis of digital volumes

In this paper, we provide a graph-based representation of the homology (information related to the different “holes” the object has) of a binary digital volume. We analyze the digital volume AT-model representation [8] from this point of view and the cellular version of the AT-model [5] is precisely described here as three forests (connectivity forests), from which, for instance, we can straightforwardly determine representative curves of “tunnels” and “holes”, classify cycles in the complex, computing higher (co)homology operations,... Depending of the order in which we gradually construct these trees, tools so important in Computer Vision and Digital Image Processing as Reeb graphs and topological skeletons appear as results of pruning these graphs

Changes in wave climate over the northwest European shelf seas during the last 12,000 years

Because of the depth attenuation of wave orbital velocity, wave-induced bed shear stress is much more sensitive to changes in total water depth than tidal-induced bed shear stress. The ratio between wave- and tidal-induced bed shear stress in many shelf sea regions has varied considerably over the recent geological past because of combined eustatic changes in sea level and isostatic adjustment. In order to capture the high-frequency nature of wind events, a two-dimensional spectral wave model is here applied at high temporal resolution to time slices from 12 ka BP to present using paleobathymetries of the NW European shelf seas. By contrasting paleowave climates and bed shear stress distributions with present-day conditions, the model results demonstrate that, in regions of the shelf seas that remained wet continuously over the last 12,000 years, annual root-mean-square (rms) and peak wave heights increased from 12 ka BP to present. This increase in wave height was accompanied by a large reduction in the annual rms wave- induced bed shear stress, primarily caused by a reduction in the magnitude of wave orbital velocity penetrating to the bed for increasing relative sea level. In regions of the shelf seas which remained wet over the last 12,000 years, the annual mean ratio of wave- to (M-2) tidal-induced bed shear stress decreased from 1 (at 12 ka BP) to its present-day value of 0.5. Therefore compared to present- day conditions, waves had a more important contribution to large-scale sediment transport processes in the Celtic Sea and the northwestern North Sea at 12 ka BP

Removal operations in nD generalized maps for efficient homology computation

In this paper, we present an efficient way for computing homology generators of nD generalized maps. The algorithm proceeds in two steps: (1) cell removals reduces the number of cells while preserving homology; (2) homology generator computation is performed on the reduced object by reducing incidence matrices into their Smith-Agoston normal form. In this paper, we provide a definition of cells that can be removed while preserving homology. Some results on 2D and 3D homology generators computation are presented

Advanced homology computation of digital volumes via cell complexes

Given a 3D binary voxel-based digital object V, an algorithm for computing homological information for V via a polyhedral cell complex is designed. By homological information we understand not only Betti numbers, representative cycles of homology classes and homological classification of cycles but also the computation of homology numbers related additional algebraic structures defined on homology (coproduct in homology, product in cohomology, (co)homology operations,...). The algorithm is mainly based on the following facts: a) a local 3D-polyhedrization of any 2×2×2 configuration of mutually 26-adjacent black voxels providing a coherent cell complex at global level; b) a description of the homology of a digital volume as an algebraic-gradient vector field on the cell complex (see Discrete Morse Theory [5], AT-model method [7,5]). Saving this vector field, we go further obtaining homological information at no extra time processing cost

Influence of block versus random monomer distribution on the cellular uptake of hydrophilic copolymers

The use of polymers has revolutionized the field of drug delivery in the past two decades. Properties such as polymer size, charge, hydrophilicity, or branching have all been shown to play an important role in the cellular internalization of polymeric systems. In contrast, the fundamental impact of monomer distribution on the resulting biological properties of copolymers remains poorly studied and is always only investigated for biologically active self-assembling polymeric systems. Here, we explore the fundamental influence of monomer distribution on the cellular uptake of nonaggregating and biologically passive copolymers. Reversible addition–fragmentation chain-transfer (RAFT) polymerization was used to prepare precisely defined copolymers of three hydrophilic acrylamide monomers. The cellular internalization of block copolymers was compared with the uptake of a random copolymer where monomers are statistically distributed along the chain. The results demonstrate that monomer distribution in itself has a negligible impact on copolymer uptake

On the Expressivity and Applicability of Model Representation Formalisms

A number of first-order calculi employ an explicit model representation formalism for automated reasoning and for detecting satisfiability. Many of these formalisms can represent infinite Herbrand models. The first-order fragment of monadic, shallow, linear, Horn (MSLH) clauses, is such a formalism used in the approximation refinement calculus. Our first result is a finite model property for MSLH clause sets. Therefore, MSLH clause sets cannot represent models of clause sets with inherently infinite models. Through a translation to tree automata, we further show that this limitation also applies to the linear fragments of implicit generalizations, which is the formalism used in the model-evolution calculus, to atoms with disequality constraints, the formalisms used in the non-redundant clause learning calculus (NRCL), and to atoms with membership constraints, a formalism used for example in decision procedures for algebraic data types. Although these formalisms cannot represent models of clause sets with inherently infinite models, through an additional approximation step they can. This is our second main result. For clause sets including the definition of an equivalence relation with the help of an additional, novel approximation, called reflexive relation splitting, the approximation refinement calculus can automatically show satisfiability through the MSLH clause set formalism.Comment: 15 page