Odontoameloblastoma: descripción de un caso y revisión de la literatura

El odontoameloblastoma (OA) es un tumor odontogénico mixto extremadamente raro que aparece en los huesos maxilares y que presenta tanto componentes epiteliales como mesenquimales. El término odontoameloblastoma fue incluido en la clasificación de 1971 de la OMS. Tan solo 23 casos bien documentados han sido publicados. Debido a su rareza, existe controversia en cuanto al tratamiento de este tumor. Presentamos un nuevo caso de OA que afecta a la mandíbula y simula un odontoma compuesto, así como una breve revisión de la literatura.Odontoameloblastoma (OA) is an extremely rare mixed odontogenic tumor appearing within the maxillary bone, with both epithelial and mesenchymal components. The term odontoameloblastoma (OA) was included in the 1971's WHO classification. Only 23 well-documented cases have been reported in the medical literature. Because of their rarity, controversy exists in the treatment of this tumor. We present a new case of OA involving the mandible mimicking a compound odontoma and a brief review of the related literature

Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus

The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virio

Analysis of adaptive responses of Pinus pinaster to changing environmental conditions in the Mediterranean region

Recent climate evolution studies highlight the progressive temperature increase and prevalence of seasonal drought, with specially incidence in the Mediterranean region. Although conifers are very important species regarding forest conservation, sustainability and productivity, given the large forest surface they cover in Spain and their active role in preventing soil erosion and desertification, we know little about the molecular mechanisms which control adaptation in this ancient taxonomic grou

Efecto de la fertilización nitrogenada en pasturas consociadas

En los sistemas de producción ganaderos argentinos la aplicación de fertilizantes está menos difundida que en los agrícolas. La aplicación de N en otoño adelanta el crecimiento de las gramíneas y, por lo tanto, el pastoreo, en un momento estratégico de oferta forrajera en general limitante. Las leguminosas tienen la capacidad de fijar grandes cantidades de N atmosférico y transferir parte del mismo a las gramíneas acompañantes o a cultivos posteriores. En Entre Ríos es escasa la información sobre el impacto de la fertilización nitrogenada en pasturas consociadas con base leguminosa. A partir de ensayos instalados en sistemas reales de producción se evaluó el impacto productivo en función de dosis y momentos de agregado de N en pasturas consociadas de distinta antigüedad y composición. La respuesta y la eficiencia de uso del N agregado fue dependiente de la composición de la pastura. La respuesta al agregado de N en pasturas con bajo porcentaje de gramínea (<70%) puede no ser viable económicamente. Las aplicaciones divididas de N no generaron una mayor producción de forraje.EEA ParanáFil: Pautasso, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná. Agencia de Extensión Rural Diamante; ArgentinaFil: Pautasso, Juan Manuel. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Barbagelata, Pedro Anibal. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina.Fil: Giordano, Matías J. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Peltzer, Y.H. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Cian, María Marcela. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Lorenzon, Marina de las Mercedes. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentin

Semaphorin 4B is an ADAM17-cleaved adipokine that inhibits adipocyte differentiation and thermogenesis

Objective: The metalloprotease ADAM17 (also called TACE) plays fundamental roles in homeostasis by shedding key signaling molecules from the cell surface. Although its importance for the immune system and epithelial tissues is well-documented, little is known about the role of ADAM17 in metabolic homeostasis. The purpose of this study was to determine the impact of ADAM17 expression, specifically in adipose tissues, on metabolic homeostasis.Methods: We used histopathology, molecular, proteomic, transcriptomic, in vivo integrative physiological and ex vivo biochemical approaches to determine the impact of adipose tissue-specific deletion of ADAM17 upon adipocyte and whole organism metabolic physiology.Results: ADAM17adipoq-creD/D mice exhibited a hypermetabolic phenotype characterized by elevated energy consumption and increased levels of adipocyte thermogenic gene expression. On a high fat diet, these mice were more thermogenic, while exhibiting elevated expression levels of genes associated with lipid oxidation and lipolysis. This hypermetabolic phenotype protected mutant mice from obesogenic challenge, limiting weight gain, hepatosteatosis and insulin resistance. Activation of beta-adrenoceptors by the neurotransmitter norepinephrine, a key regulator of adipocyte physiology, triggered the shedding of ADAM17 substrates, and regulated ADAM17 expression at the mRNA and protein levels, hence identifying a functional connection between thermogenic licensing and the regulation of ADAM17. Proteomic studies identified Semaphorin 4B (SEMA4B), as a novel ADAM17-shed adipokine, whose expression is regulated by physiological thermogenic cues, that acts to inhibit adipocyte differentiation and dampen thermogenic responses in adipocytes. Transcriptomic data showed that cleaved SEMA4B acts in an autocrine manner in brown adipocytes to repress the expression of genes involved in adipogenesis, thermogenesis, and lipid uptake, storage and catabolism.Conclusions: Our findings identify a novel ADAM17-dependent axis, regulated by beta-adrenoceptors and mediated by the ADAM17-cleaved form of SEMA4B, that modulates energy balance in adipocytes by inhibiting adipocyte differentiation, thermogenesis and lipid catabolism.(c) 2023 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Trajectories of alcohol consumption during life and the risk of developing breast cancer

Background Whether there are lifetime points of greater sensitivity to the deleterious effects of alcohol intake on the breasts remains inconclusive. Objective To compare the influence of distinctive trajectories of alcohol consumption throughout a woman's life on development of breast cancer (BC). Methods 1278 confirmed invasive BC cases and matched (by age and residence) controls from the Epi-GEICAM study (Spain) were used. The novel group-based trajectory modelling was used to identify different alcohol consumption trajectories throughout women's lifetime. Results Four alcohol trajectories were identified. The first comprised women (45%) with low alcohol consumption (= 15 g/day), never having a low alcohol consumption. Comparing with the first trajectory, the fourth doubled BC risk (OR 2.19; 95% CI 1.27, 3.77), followed by the third (OR 1.44; 0.96, 2.16) and ultimately by the second trajectory (OR 1.17; 0.86, 1.58). The magnitude of BC risk was greater in postmenopausal women, especially in those with underweight or normal weight. When alcohol consumption was independently examined at each life stage, >= 15 g/day of alcohol consumption in adolescence was strongly associated with BC risk followed by consumption in adulthood. Conclusions The greater the alcohol consumption accumulated throughout life, the greater the risk of BC, especially in postmenopausal women. Alcohol consumption during adolescence may particularly influence BC risk.This study was funded by the Fundacion Cientifica Asociacion Espanola Contra el Cancer (AECC) (Scientific Foundation of the Spanish Association against Cancer 2006 & 2016) (Marina Pollan), Sociedad Espanola de Oncologia Medica (SEOM) (Spanish Society of Medical Oncology) (Miguel Martin), Scholarship 'Contrato de atraccion de talento' from Community of Madrid (Carolina Donat-Vargas), Fundacion Cerveza y Salud 2005 (Beer and Health Foundation 2005) (Miguel Martin) and Federacion de Asociaciones de Mujeres con Cancer de Mama (FECMA) (Spanish Federation of Associations of Women with Breast Cancer) (Miguel Martin, Marina Pollan)

Primary breast cancer and health related quality of life in Spanish women: The EpiGEICAM case-control study

This study evaluates the impact of breast cancer (BC) in health related quality of life (HRQL) and in psychological distress (PD) during the initial phases of the disease and looks for contributing factors. A multicentric case-control study, EpiGEICAM, was carried out. Incident BC cases and age- and residence- matched controls were included. Clinical, epidemiological, HRQL (SF-36) and PD information (GHQ-28) was collected. We used multivariable logistic regression models to estimate OR of low HRQL and of PD in cases compared to controls, and to identify factors associated with low HRQL and with PD. Among 896 BC cases and 890 control women, cases had poorer scores than both, the reference population and the control group, in all SF-36 scales. BC women with lower education, younger, active workers, never smokers, those with comorbidities, in stage IV and with surgical treatment had lower physical HRQL; factors associated with low mental HRQL were dissatisfaction with social support, being current smoker and having children. Cases had a fivefold increased odds of PD compared to controls. Managing comorbidities and trying to promote social support, especially in younger and less educated women, could improve well-being of BC patients

Serum Phospholipids Fatty Acids and Breast Cancer Risk by Pathological Subtype

This study evaluates whether serum phospholipids fatty acids (PL-FAs) and markers of their endogenous metabolism are associated with breast cancer (BC) subtypes. EpiGEICAM is a Spanish multicenter matched case-control study. A lifestyle and food frequency questionnaire was completed by 1017 BC cases and healthy women pairs. Serum PL-FA percentages were measured by gas chromatography-mass spectrometry. Conditional and multinomial logistic regression models were used to quantify the association of PL-FA tertiles with BC risk, overall and by pathological subtype (luminal, HER2+ and triple negative). Stratified analyses by body mass index and menopausal status were also performed. Serum PL-FAs were measured in 795 (78%) pairs. Women with high serum levels of stearic acid (odds ratio (OR)T3vsT1 = 0.44; 95% confidence interval (CI) = 0.30-0.66), linoleic acid (ORT3vsT1 = 0.66; 95% CI = 0.49-0.90) and arachidonic to dihomo-γ-linolenic acid ratio (OR T3vsT1 = 0.64; 95% CI = 0.48-0.84) presented lower BC risk. Participants with high concentrations of palmitoleic acid (ORT3vsT1 = 1.65; 95% CI = 1.20-2.26), trans-ruminant palmitelaidic acid (ORT3vsT1 = 1.51; 95% CI = 1.12-2.02), trans-industrial elaidic acid (ORT3vsT1 = 1.52; 95% CI = 1.14-2.03), and high oleic to stearic acid ratio (ORT3vsT1 = 2.04; 95% CI = 1.45-2.87) showed higher risk. These associations were similar in all BC pathological subtypes. Our results emphasize the importance of analyzing fatty acids individually, as well as the desaturase activity indices

A social-ecological approach to identify and quantify biodiversity tipping points in South America’s seasonal dry ecosystems

ropical dry forests and savannas harbour unique biodiversity and provide critical ES, yet they are under severe pressure globally. We need to improve our understanding of how and when this pressure provokes tipping points in biodiversity and the associated social-ecological systems. We propose an approach to investigate how drivers leading to natural vegetation decline trigger biodiversity tipping and illustrate it using the example of the Dry Diagonal in South America, an understudied deforestation frontier. The Dry Diagonal represents the largest continuous area of dry forests and savannas in South America, extending over three million km² across Argentina, Bolivia, Brazil, and Paraguay. Natural vegetation in the Dry Diagonal has been undergoing large-scale transformations for the past 30 years due to massive agricultural expansion and intensification. Many signs indicate that natural vegetation decline has reached critical levels. Major research gaps prevail, however, in our understanding of how these transformations affect the unique and rich biodiversity of the Dry Diagonal, and how this affects the ecological integrity and the provisioning of ES that are critical both for local livelihoods and commercial agriculture.Fil: Thonicke, Kirsten. Institute for Climate Impact Research ; AlemaniaFil: Langerwisch, Fanny. Institute for Climate Impact Research ; Alemania. Czech University of Life Sciences Prague; República ChecaFil: Baumann, Matthias. Humboldt Universität zu Berlin; Alemania. Technische Universitat Carolo Wilhelmina Zu Braunschweig.; AlemaniaFil: Leitão, Pedro J.. Humboldt Universität zu Berlin; Alemania. Technische Universitat Carolo Wilhelmina Zu Braunschweig.; AlemaniaFil: Václavík, Tomáš. Helmholtz Centre for Environmental Research; Alemania. Palacký University Olomouc; República ChecaFil: Alencar, Anne. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Simões, Margareth. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA); BrasilFil: Scheiter, Simon. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA); Brasil. Universidade Federal do Rio de Janeiro; BrasilFil: Langan, Liam. Senckenberg Biodiversity and Climate Research Centre; AlemaniaFil: Bustamante, Mercedes. Universidade do Brasília; BrasilFil: Gasparri, Nestor Ignacio. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto de Ecología Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Hirota, Marina. Universidade Federal de Santa Catarina; Brasil. Universidade Estadual de Campinas; BrasilFil: Börner, Jan. Universitat Bonn; AlemaniaFil: Rajao, Raoni. Universidade Federal de Minas Gerais; BrasilFil: Soares Filho, Britaldo. Universidade Federal de Minas Gerais; BrasilFil: Yanosky, Alberto. Consejo Nacional de Ciencia y Tecnología; ParaguayFil: Ochoa Quinteiro, José Manuel. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt; ColombiaFil: Seghezzo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Conti, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: de la Vega Leiner, Anne Cristina. Universität Greifswald; Alemani

Clinical and Sociodemographic Determinants of Adherence to World Cancer Research Fund/American Institute for Cancer Research (WCRF/AICR) Recommendations in Breast Cancer Survivors—Health-EpiGEICAM Study

Breast cancer (BC) survivors are advised to follow the WCRF/AICR cancer prevention recommendations, given their high risk of developing a second tumour. We aimed to explore compliance with these recommendations in BC survivors and to identify potentially associated clinical and sociodemographic factors. A total of 420 BC survivors, aged 31–80, was recruited from 16 Spanish hospitals. Epidemiological, dietary and physical activity information was collected through questionnaires. A 7-item score to measure compliance with the recommendations was built according to the 2018 WCRF/AICR scoring criteria. Standardized prevalences and standardized prevalence ratios of moderate and high compliance across participant characteristics were estimated using multinomial and binary logistic regression models. The mean score was 3.9 (SD: 1.0) out of 7 points. Recommendations with the worst adherence were those of limiting consumption of red/processed meats (12% of compliance, 95% CI: 8.2–15.0) and high fibre intake (22% of compliance, 95% CI: 17.6–27.0), while the best compliance was observed for the consumption of fruits and vegetables (73% of compliance, 95% CI: 69.2–77.7). Overall, adherence was worse in women with university education and in those with first-degree relatives with BC. This information may be of interest to design and implement personalized preventive measures adapted to the characteristics of these patients19 página