BONE RESORPTION MARKER BETA-CROSSLAPS FOR EARLY MONITORING OF OSTEOPOROSIS TREATMENT WITH DENOSUMAB

Antiresorptive long-term treatment for osteoporosis increases bone mineral density. Due to the long duration, poor compliance of patients to medication therapy is a big challenge. Bone mineral density measurements are recommended for the monitoring of treatment, but detectable changes may take up to 2-3 years. Bone turnover markers can indicate changes in bone turnover rates earlier, and assessment their levels may be effective in enhancing compliance and clinical efficacy. Purpose: Our study aimed to assess whether the dynamics in serum levels of bone resorption marker beta-CrossLaps have a real practical benefit for early monitoring of postmenopausal osteoporosis treatment. Materials and methods: 21 Bulgarian women in menopause with newly diagnosed osteoporosis were studied. All participants hadn’t been under treatment. Serum beta-CrossLaps levels were measured before and six months after subcutaneous administration of Denosumab – Injection 60 mg. Results: Serum concentrations before and six months after starting treatment were respectively 0.589 ± 0.266 ng/ml (0.06 – 1.2) and 0.166 ± 0.139 ng/ml (0.05 – 0.59). The beta-CrossLaps pre-treatment serum levels were within the reference range for the commercial kit used. After six months of treatment, there was a significant decrease in serum concentrations of about 72% from baseline. In our study, although pre-treatment levels were within the reference range, a significant decrease in concentrations was observed. Conclusions: The results show that the dynamics of beta-CrossLaps may be useful in the early monitoring of osteoporosis treatment

Ochratoxin A and β2-Microglobulin in BEN Patients and Controls

Ochratoxin A (OTA) is a mycotoxin naturally occurring in different foods. OTA is arguably a risk factor for Balkan endemic nephropathy (BEN). The aims of this study are to (1) test the OTA-BEN association in BEN-groups and controls and (2) determine whether urine β2-microglobulin, a marker of impaired ability of the kidneys to re-absorb, is related to OTA. BEN patients had significantly higher OTA serum levels. Within the offspring, OTA was significantly related to higher β2-microglobulin excretion. OTA (2005/2006) was related to a higher incidence of BEN after 2008, providing further evidence that OTA is a risk factor for BEN

Influence of chemical structure and mechanism of hydrolysis on pharmacological activity and toxicological profile of approved platinum drugs

The problems with platinum complexes are resistance and toxicity of anticancer therapy. The aim of current study is the comparison of the influence of chemical structure and mechanism of hydrolysis on pharmacological activity and toxicological profile of approved in platinum drugs: Cisplatin, Carboplatin, Oxaliplatin, Nedaplatin, Lobaplatin, Heptaplatin, Satraplatin. Hydrolysis of Carboplatin and Nedaplatin occurs by double step hydration, to obtain the same active products as with Cisplatin: diaqudiamine-platinum. The similarity in mechanisms of hydrolysis of Oxaliplatin, Lobaplatin Heptaplatin, and Satraplatin is that the first part of the hydrolysis corresponds to the ring-opening and addition of the first water molecule, and in the second step of reaction occur the loss of the ligand and the formation of the di-aquated product by the addition of a second water molecule. Cisplatin, Carboplatin, and Oxaliplatin are nephrotoxic. Cisplatin and Heptaplatin are nephrotoxic. The similar dose-limiting effects of Carboplatin, Oxaliplatin, Nedaplatin, Lobaplatin, and Satraplatin is myelosuppression

Immunogenesis in patients with medium and severe coronavirus infection – dynamics in different age groups

The results of a one-year prospective study, during which the process of immunogenesis in patients over 18 years of age with moderate and severe coronavirus infection was monitored and analyzed in clinical and paraclinical (clinical laboratory) aspects, are summarized and presented. The study included 2683 patients, all treated in the Clinic of Internal Diseases at the University Multiprofile Hospital for Active Treatment and Emergency Medicine “N. I. Pirogov” EAD, Sofia for the period from April 2020 to December 2020. Patients were followed for one year after recovering from moderate to severe coronavirus infection. Patients are grouped into four age categories as follows: 18–45 years; 46–65 years; 66–80 years and over 80 years. The results of our study show that during the study period in 97% of patients the level of anti-SARS-CoV2, rose and in the remaining three percent it was flat or followed by subsequent waning (in less than 1% of patients), but does not reach critically low levels (i. e. below the positivity conditional threshold). The level of IgG reached a peak and then waned, but on the other hand, as mentioned above, the amount of Ig-Total tested shows a significant increase. This trend is observed in all age groups, with a difference in the level of IgG and Ig-Total depending on age. The results of the additional screening in the target period in terms of virulence and virus segregation, categorically rule out the suspicion of the presence of “silent spreader”. During the follow-up period, no patients were re-hospitalized due to recurrence of Coronavirus infection (re-infection and illness)

Immunogenesis in patients with medium and severe coronavirus infection – dynamics in different age groups

The results of a one-year prospective study, during which the process of immunogenesis in patients over 18 years of age with moderate and severe coronavirus infection was monitored and analyzed in clinical and paraclinical (clinical laboratory) aspects, are summarized and presented. The study included 2683 patients, all treated in the Clinic of Internal Diseases at the University Multiprofile Hospital for Active Treatment and Emergency Medicine “N. I. Pirogov” EAD, Sofia for the period from April 2020 to December 2020. Patients were followed for one year after recovering from moderate to severe coronavirus infection. Patients are grouped into four age categories as follows: 18–45 years; 46–65 years; 66–80 years and over 80 years. The results of our study show that during the study period in 97% of patients the level of anti-SARS-CoV2, rose and in the remaining three percent it was flat or followed by subsequent waning (in less than 1% of patients), but does not reach critically low levels (i. e. below the positivity conditional threshold). The level of IgG reached a peak and then waned, but on the other hand, as mentioned above, the amount of Ig-Total tested shows a significant increase. This trend is observed in all age groups, with a difference in the level of IgG and Ig-Total depending on age. The results of the additional screening in the target period in terms of virulence and virus segregation, categorically rule out the suspicion of the presence of “silent spreader”. During the follow-up period, no patients were re-hospitalized due to recurrence of Coronavirus infection (re-infection and illness)

Втора научна сесия за преподаватели и студенти 3-4 Октомври 2013, Варна

Варненски медицински форум (Varna Medical Forum) V. 2, Suppl 3(2013