Uncertainties in TOC retrieval for Brewer and Dobson data and the role of cross-correlations among influence parameters

Presentación realizada en: ATMOZ workshop at 11th RBCC-E, celebrado en El Arenosillo, Huelva, el 1 de junio de 2017

Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica

Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA

Gre factors-mediated control of hilD transcription is essential for the invasion of epithelial cells by Salmonella enterica serovar Typhimurium

The invasion of epithelial cells by Salmonella enterica serovar Typhimurium is a very tightly regulated process. Signaling cascades triggered by different environmental and physiological signals converge to control HilD, an AraC regulator that coordinates the expression of several virulence factors. The expression of hilD is modulated at several steps of the expression process. Here, we report that the invasion of epithelial cells by S. Typhimurium strains lacking the Gre factors, GreA and GreB, is impaired. By interacting with the RNA polymerase secondary channel, the Gre factors prevent backtracking of paused complexes to avoid arrest during transcriptional elongation. Our results indicate that the Gre factors are required for the expression of the bacterial factors needed for epithelial cell invasion by modulating expression of HilD. This regulation does not occur at transcription initiation and depends on the capacity of the Gre factors to prevent backtracking of the RNA polymerase. Remarkably, genetic analyses indicate that the 3'-untranslated region (UTR) of hilD is required for Gre-mediated regulation of hilD expression. Our data provide new insight into the complex regulation of S. Typhimurium virulence and highlight the role of the hilD 3'-UTR as a regulatory motif

Reconstructing complex lineage trees from scRNA-seq data using MERLoT

Advances in single-cell transcriptomics techniques are revolutionizing studies of cellular differentiation and heterogeneity. It has become possible to track the trajectory of thousands of genes across the cellular lineage trees that represent the temporal emergence of cell types during dynamic processes. However, reconstruction of cellular lineage trees with more than a few cell fates has proved challenging. We present MERLoT (https://github.com/soedinglab/merlot), a flexible and user-friendly tool to reconstruct complex lineage trees from single-cell transcriptomics data. It can impute temporal gene expression profiles along the reconstructed tree. We show MERLoT’s capabilities on various real cases and hundreds of simulated datasets

Estudio de viabilidad de mercado para la importación de aceites y lubricantes en Cuenca - Ecuador.

Entrepreneurship, business creation, search for new markets, generation of niches, product innovation, drives the continuous research of their feasibility. The present investigation consists of the realization of the commercial viability for the import of oils and lubricants in Cuenca - Ecuador, within the administration, the formulation of projects and the analyses that arise from the management, are indispensable tools, for which the study includes a mixed analysis generating in the process a synthetic analytical method realized through primary information obtained through a sampling of consumption of oils and lubricants for automotive, that allows to demonstrate the increase of the market of oils and lubricants in the province of Azuay; Also to know the opportunity to enter the market with innovation of brands of automotive lubricants determined by an unsatisfied demand, which will demonstrate the viability of the venture and its success.El emprendimiento, la creación de negocios, búsqueda de nuevos mercados, generación de nichos, innovación en productos, impulsa a la contínua investigación de la factibilidad de los mismos. La presente investigación consta de la realización de la viabilidad comercial para la importación de aceites y lubricantes en Cuenca - Ecuador, dentro de la administración, la formulación de proyectos y los análisis que se desprenden de la gestión, son herramientas indispensables, por lo cual el estudio comprende un análisis mixto generando en el proceso un método analítico sintético realizado a través de información primaria obtenida a través de una muestreo de consumo de aceites y lubricantes para automotor, que permite demostrar el incremento de mercado de aceites y lubricantes en la provincia del Azuay; conocer además la oportunidad de ingresar al mercado con innovación de marcas de lubricantes para automotor determinado por una demanda insatisfecha, lo cual permitirá evidenciar la viabilidad del emprendimiento y su éxito

Mean square solution of Bessel differential equation with uncertainties

[EN] This paper deals with the study of a Bessel-type differential equation where input parameters (coefficient and initial conditions) are assumed to be random variables. Using the so-called Lp-random calculus and assuming moment conditions on the random variables in the equation, a mean square convergent generalized power series solution is constructed. As a result of this convergence, the sequences of the mean and standard deviation obtained from the truncated power series solution are convergent as well. The results obtained in the random framework extend their deterministic counterpart. The theory is illustrated in two examples in which several distributions on the random inputs are assumed. Finally, we show through examples that the proposed method is computationally faster than Monte Carlo method.This work has been partially supported by the Spanish Ministerio de Economía y Competitividad grant MTM2013-41765-P and by the European Union in the FP7-PEOPLE-2012-ITN Program under Grant Agreement No. 304617 (FP7 Marie Curie Action, Project Multi-ITN STRIKE-Novel Methods in Computational Finance) and Mexican Conacyt.Cortés, J.; Jódar Sánchez, LA.; Villafuerte, L. (2017). Mean square solution of Bessel differential equation with uncertainties. Journal of Computational and Applied Mathematics. 309:383-395. https://doi.org/10.1016/j.cam.2016.01.034S38339530

Biocompatibility and corrosion protection behaviour of hydroxyapatite sol-gel-derived coatings on Ti6Al4V alloy

-2,5-diphenyl tetrazolium bromide (MTT) and Alamar blue cell viability assays were used to study the biocompatibility. Finally, the corrosion behaviour of HAp-coatings/Ti6Al4V system was researched by means of Electrochemical Impedance Spectroscopy (EIS). The obtained results showed that the prepared powders were nanocrystalline HAp with little deviations from that present in the human bone. All the prepared HAp coatings deposited on Ti6Al4V showed well-behaved biocompatibility, good bioactivity and corrosion protection properties.The authors would like to acknowledge financial support from the Ministry of Economy and Competitiveness of Spain (MAT2012-30854 and MAT2015-65445-C2-1-R Projects)

Maternal Serum Meteorin Levels and the Risk of Preeclampsia

BACKGROUND: Meteorin (METRN) is a recently described neutrophic factor with angiogenic properties. This is a nested case-control study in a longitudinal cohort study that describes the serum profile of METRN during different periods of gestation in healthy and preeclamptic pregnant women. Moreover, we explore the possible application of METRN as a biomarker. METHODS AND FINDINGS: Serum METRN was measured by ELISA in a longitudinal prospective cohort study in 37 healthy pregnant women, 16 mild preeclamptic women, and 20 healthy non-pregnant women during the menstrual cycle with the aim of assessing serum METRN levels and its correlations with other metabolic parameters. Immunostaining for METRN protein was performed in placenta. A multivariate logistic regression model was proposed and a classifier model was formulated for predicting preeclampsia in early and middle pregnancy. The performance in classification was evaluated using measures such as sensitivity, specificity, and the receiver operating characteristic (ROC) curve. In healthy pregnant women, serum METRN levels were significantly elevated in early pregnancy compared to middle and late pregnancy. METRN levels are significantly lower only in early pregnancy in preeclamptic women when compared to healthy pregnant women. Decision trees that did not include METRN levels in the first trimester had a reduced sensitivity of 56% in the detection of preeclamptic women, compared to a sensitivity of 69% when METRN was included. CONCLUSIONS: The joint measurements of circulating METRN levels in the first trimester and systolic blood pressure and weight in the second trimester significantly increase the probabilities of predicting preeclampsia

Gre factors are required fot biofilm formation in Salmonella enterica serovar Typhimurium by targeting transcription of the cgsD gene

Rdar biofilm formation of Salmonella typhimurium and Escherichia coli is a common ancient multicellular behavior relevant in cell-cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix components amyloid curli fimbriae and the exopolysaccharide cellulose, the central hub for the delicate regulation of rdar morphotype expression is the orphan transcriptional regulator CsgD. Gre factors are ubiquitously interacting with RNA polymerase to selectively overcome transcriptional pausing. In this work, we found that GreA/GreB are required for expression of the csgD operon and consequently the rdar morphotype. The ability of the Gre factors to suppress transcriptional pausing and the 147 bp 5′-UTR of csgD are required for the stimulatory effect of the Gre factors on csgD expression. These novel mechanism(s) of regulation for the csgD operon might be relevant under specific stress conditions

Chromogenic Chemodosimeter Based on Capped Silica Particles to Detect Spermine and Spermidine

[EN] A new hybrid organic-inorganic material for sensing spermine (Spm) and spermidine (Spd) has been prepared and characterized. The material is based on MCM-41 particles functionalized with an N-hydroxysuccinimide derivative and loaded with Rhodamine 6G. The cargo is kept inside the porous material due to the formation of a double layer of organic matter. The inner layer is covalently bound to the silica particles, while the external layer is formed through hydrogen and hydrophobic interactions. The limits of detection determined by fluorimetric titration are 27 mu M and 45 mu M for Spm and Spd, respectively. The sensor remains silent in the presence of other biologically important amines and is able to detect Spm and Spd in both aqueous solution and cells.This research was funded by Spanish Government (RTI2018-100910-B-C42 and RTI2018100910-B-C44 (MCUI/AEI/FEDER, UE) and grant GRISOLIAP/2019/023.Barros, M.; López-Carrasco, A.; Amorós, P.; Gil Grau, S.; Gaviña, P.; Parra Alvarez, M.; El Haskouri, J.... (2021). Chromogenic Chemodosimeter Based on Capped Silica Particles to Detect Spermine and Spermidine. Nanomaterials. 11(3):1-12. https://doi.org/10.3390/nano1103081811211