Radio observations of evaporating objects in the Cygnus OB2 region

We present observations of the Cygnus OB2 region obtained with the Giant Metrewave Radio Telescope (GMRT) at frequencies of 325 and 610 MHz. In this contribution we focus on the study of proplyd-like objects (also known as free-floating evaporating gas globules or frEGGs) that typically show an extended cometary morphology. We identify eight objects previously studied at other wavelengths and derive their physical properties by obtaining their optical depth at radio-wavelengths. Using their geometry and the photoionization rate needed to produce their radio-continuum emission, we find that these sources are possibly ionized by a contribution of the stars Cyg OB2 #9 and Cyg OB2 #22. Spectral index maps of the eight frEGGs were constructed, showing a flat spectrum in radio frequencies in general. We interpret these as produced by optically thin ionized gas, although it is possible that a combination of thermal emission, not necessarily optically thin, produced by a diffuse gas component and the instrument response (which detects more diffuse emission at low frequencies) can artificially generate negative spectral indices. In particular, for the case of the Tadpole we suggest that the observed emission is not of non-thermal origin despite the presence of regions with negative spectral indices in our maps.Fil: Isequilla, Natacha Laura. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas; ArgentinaFil: Fernandez Lopez, Manuel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Benaglia, Paula. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Ishwara Chandra, C. H.. National Center For Radio Astrophysics; IndiaFil: del Palacio, Santiago. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas; Argentin

Kv2.1 channels play opposing roles in regulating membrane potential, Ca2+ channel function, and myogenic tone in arterial smooth muscle.

The accepted role of the protein Kv2.1 in arterial smooth muscle cells is to form K+ channels in the sarcolemma. Opening of Kv2.1 channels causes membrane hyperpolarization, which decreases the activity of L-type CaV1.2 channels, lowering intracellular Ca2+ ([Ca2+]i) and causing smooth muscle relaxation. A limitation of this model is that it is based exclusively on data from male arterial myocytes. Here, we used a combination of electrophysiology as well as imaging approaches to investigate the role of Kv2.1 channels in male and female arterial myocytes. We confirmed that Kv2.1 plays a canonical conductive role but found it also has a structural role in arterial myocytes to enhance clustering of CaV1.2 channels. Less than 1% of Kv2.1 channels are conductive and induce membrane hyperpolarization. Paradoxically, by enhancing the structural clustering and probability of CaV1.2-CaV1.2 interactions within these clusters, Kv2.1 increases Ca2+ influx. These functional impacts of Kv2.1 depend on its level of expression, which varies with sex. In female myocytes, where expression of Kv2.1 protein is higher than in male myocytes, Kv2.1 has conductive and structural roles. Female myocytes have larger CaV1.2 clusters, larger [Ca2+]i, and larger myogenic tone than male myocytes. In contrast, in male myocytes, Kv2.1 channels regulate membrane potential but not CaV1.2 channel clustering. We propose a model in which Kv2.1 function varies with sex: in males, Kv2.1 channels control membrane potential but, in female myocytes, Kv2.1 plays dual electrical and CaV1.2 clustering roles. This contributes to sex-specific regulation of excitability, [Ca2+]i, and myogenic tone in arterial myocytes

Investigation of the WR 11 field at decimeter wavelengths

The massive binary system WR 11 (γ2-Velorum) has recently been proposed as the counterpart of a Fermi source. If this association is correct, this system would be the second colliding wind binary detected in GeV γ-rays. However, the reported flux measurements from 1.4 to 8.64 GHz fail to establish the presence of nonthermal (synchrotron) emission from this source. Moreover, WR 11 is not the only radio source within the Fermi detection box. Other possible counterparts have been identified in archival data, some of which present strong nonthermal radio emission. We conducted arcsec-resolution observations toward WR 11 at very low frequencies (150-1400 MHz) where the nonthermal emission - if existent and not absorbed - is expected to dominate. We present a catalog of more than 400 radio emitters, among which a significant portion are detected at more than one frequency, including limited spectral index information. Twenty-one of these radio emitters are located within the Fermi significant emission. A search for counterparts for this last group pointed at MOST 0808-471; this source is 2´ away from WR 11 and is a promising candidate for high-energy emission, having a resolved structure along 325-1390 MHz. For this source, we reprocessed archive interferometric data up to 22.3 GHz and obtained a nonthermal radio spectral index of - 0.97 ± 0.09. However, multiwavelength observations of this source are required to establish its nature and to assess whether it can produce (part of) the observed γ-rays. WR 11 spectrum follows a spectral index of 0.74 ± 0.03 from 150 to 230 GHz, consistent with thermal emission. We interpret that any putative synchrotron radiation from the colliding-wind region of this relatively short-period system is absorbed in the photospheres of the individual components. Notwithstanding, the new radio data allowed us to derive a mass-loss rate of 2.5 × 10-5 M☉ yr-1, which, according to the latest models for γ-ray emission in WR 11, would suffice to provide the required kinetic power to feed nonthermal radiation processes.Full Table 4 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/625/A99The radio data presented in this work were obtained with the Giant Metrewave Radio Telescope (GMRT). The GMRT is operated by the National Centre for Radio Astrophysics of the Tata Institute of Fundamental Research.Fil: Benaglia, Paula. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Observatorio Astronómico de La Plata - Sede Central; ArgentinaFil: del Palacio, Santiago. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Ishwara-Chandra, C. H.. International Centre Of Theoretical Science. Tata Institute Of Fundamental Research; EspañaFil: De Becker, M.. Université de Liège; BélgicaFil: Isequilla, Natacha Laura. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Observatorio Astronómico de La Plata - Sede Central; ArgentinaFil: Saponara, Juliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; Argentin

Atrial proarrhythmic effect of lead as one of the PM10 metal components of air pollution. An in-silico study

[EN] Particulate matter (PM) is considered the most severe environmental pollution problem due to its serious effects on human health associated with an increased risk of cardiovascular morbidity and mortality. In this work, a physicochemical characterization of PM10 from the city of Medellin was developed. The results evince that lead (Pb) is one of the most abundant elements since it is present in all analyzed samples. Therefore, Pb was chosen to perform an in-silico study to assess its effects on atrial arrhythmias generation. For this purpose, we developed a model representing the Pb2+ blocking effect on the L-type calcium channel. This formulation was incorporated in a human atrial cell mathematical model and in 2D and 3D models of human atria. The simulations showed a proarrhythmic effect at high Pb2+ concentrations, through shortening of action potential duration inducing the generation of reentrant activity and atrial flutter. The results contribute to the knowledge about the cardiac physiopathological processes, triggered by lead as one of the main PM10 metal components of air pollution, that yields the generation of arrhythmias.This work was supported by Ministerio de Ciencia, Tecnologi a e Innovacion MINCIENCIAS from Colombia, through grant No. 120677757994; and by the Direccion General de Politica Cientifica de la Generalitat Valenciana PROMETEO/2020/043.Palacio, LC.; Pachajoa, DC.; Durango-Giraldo, G.; Zapata-Hernández, C.; Ugarte, JP.; Saiz Rodríguez, FJ.; Buitrago-Sierra, R.... (2021). Atrial proarrhythmic effect of lead as one of the PM10 metal components of air pollution. An in-silico study. PLoS ONE. 16(10):1-17. https://doi.org/10.1371/journal.pone.0258313S117161

Radio observations of evaporating objects in the Cygnus OB2 region

We present observations of the Cygnus OB2 region obtained with the Giant Metrewave Radio Telescope (GMRT) at frequencies of 325 and 610 MHz. In this contribution we focus on the study of proplyd-like objects (also known as free-floating evaporating gas globules or frEGGs) that typically show an extended cometary morphology. We identify eight objects previously studied at other wavelengths and derive their physical properties by obtaining their optical depth at radio-wavelengths. Using their geometry and the photoionization rate needed to produce their radio-continuum emission, we find that these sources are possibly ionized by a contribution of the stars Cyg OB2 #9 and Cyg OB2 #22. Spectral index maps of the eight frEGGs were constructed, showing a flat spectrum in radio frequencies in general. We interpret these as produced by optically thin ionized gas, although it is possible that a combination of thermal emission, not necessarily optically thin, produced by a diffuse gas component and the instrument response (which detects more diffuse emission at low frequencies) can artificially generate negative spectral indices. In particular, for the case of the Tadpole we suggest that the observed emission is not of non-thermal origin despite the presence of regions with negative spectral indices in our maps.Facultad de Ciencias Astronómicas y GeofísicasInstituto Argentino de Radioastronomí

Cell free circulating tumor DNA in cerebrospinal fluid detects and monitors central nervous system involvement of B-cell lymphomas

The levels of cell free circulating tumor DNA (ctDNA) in plasma correlate with treatment response and outcome in systemic lymphomas. Notably, in brain tumors, the levels of ctDNA in the cerebrospinal fluid (CSF) are higher than in plasma. Nevertheless, their role in central nervous system (CNS) lymphomas remains elusive. We evaluated the CSF and plasma from 19 patients: 6 restricted CNS lymphomas, 1 systemic and CNS lymphoma, and 12 systemic lymphomas. We performed whole exome sequencing or targeted sequencing to identify somatic mutations of the primary tumor, then variant-specific droplet digital polymerase chain reaction was designed for each mutation. At time of enrollment, we found ctDNA in the CSF of all patients with restricted CNS lymphoma but not in patients with systemic lymphoma without CNS involvement. Conversely, plasma ctDNA was detected in only 2 out of 6 patients with restricted CNS lymphoma with lower variant allele frequencies than CSF ctDNA. Moreover, we detected CSF ctDNA in one patient with CNS lymphoma in complete remission and in one patient with systemic lymphoma, 3 and 8 months before CNS relapse was confirmed, indicating that CSF ctDNA might detect CNS relapse earlier than conventional methods. Finally, in two cases with CNS lymphoma, CSF ctDNA was still detected after treatment even though no tumoral cells were observed by flow cytometry (FC), indicating that CSF ctDNA detected residual disease better than FC. In conclusion, CSF ctDNA can detect CNS lesions better than plasma ctDNA and FC. In addition, CSF ctDNA predicted CNS relapse in CNS and systemic lymphomas

Implicaciones metodológicas e inconsistencias de la Tercera Comunicación Nacional sobre Cambio Climático de Colombia

Las Comunicaciones Nacionales sobre Cambio Climático (CNCC) son un mecanismo para que los países informen sus avances en mitigación y adaptación, y constituyen uno de los elementos de base para la política sobre cambio climático a escala nacional. Colombia ha emitido tres CNCC. La tercera plantea un escenario que considera las proyecciones de diversos modelos incluidos en la quinta fase del Proyecto de Comparación de Modelos Acoplados (Coupled Model Intercomparison Project, CMIP), el cual se estima como el promedio de las proyecciones correspondientes a las cuatro trayectorias de concentración representativa (Representative Concentration Pathways,RCP) presentadas en el quinto reporte de evaluación del Panel Intergubernamental sobre Cambio Climático. Cada una de estas RCP representa una trayectoria de concentración de gases de efecto invernadero (GEI) para un escenario particular de crecimiento poblacional, económico y tecnológico que conduce a una posible trayectoria de evolución del sistema climático. En este estudio se comparan las proyecciones presentadas en la Tercera CNCC con las obtenidas directamente de los modelos empleados. Nuestros resultados demuestran que al utilizarse un promedio de RCP se pierden escenarios alternos que podrían ser importantes a la hora de considerar posibles futuros diferentes y anulan la utilidad de plantear diversas trayectorias de emisiones de GEI. Más aun, una comparación entre la Segunda y la Tercera CNCC muestra proyecciones de precipitación opuestas para diferentes regiones del país, lo cual es de particular importancia, pues el escenario de cambio climático planteado en la Tercera CNCC sirve de referencia para la toma de decisiones en materia de cambio climático a nivel nacional

Common variants in Alzheimer’s disease and risk stratification by polygenic risk scores

Funder: Funder: Fundación bancaria ‘La Caixa’ Number: LCF/PR/PR16/51110003 Funder: Grifols SA Number: LCF/PR/PR16/51110003 Funder: European Union/EFPIA Innovative Medicines Initiative Joint Number: 115975 Funder: JPco-fuND FP-829-029 Number: 733051061Genetic discoveries of Alzheimer's disease are the drivers of our understanding, and together with polygenetic risk stratification can contribute towards planning of feasible and efficient preventive and curative clinical trials. We first perform a large genetic association study by merging all available case-control datasets and by-proxy study results (discovery n = 409,435 and validation size n = 58,190). Here, we add six variants associated with Alzheimer's disease risk (near APP, CHRNE, PRKD3/NDUFAF7, PLCG2 and two exonic variants in the SHARPIN gene). Assessment of the polygenic risk score and stratifying by APOE reveal a 4 to 5.5 years difference in median age at onset of Alzheimer's disease patients in APOE ɛ4 carriers. Because of this study, the underlying mechanisms of APP can be studied to refine the amyloid cascade and the polygenic risk score provides a tool to select individuals at high risk of Alzheimer's disease

New insights into the genetic etiology of Alzheimer's disease and related dementias

Characterization of the genetic landscape of Alzheimer's disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/'proxy' AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele