GERAÇÃO X Y Z E ALPHA – AÇÕES PARA PRESERVAÇÃO DO MEIO AMBIENTE ATRAVÉS DA EDUCAÇÃO

With the advent of globalization, communication access tools grow every day. Almost instantly, the search continues to improve the use of modern search technologies, making information faster and more assertive in the tireless search for qualification of these audiences. Thus, as a way to understand the characteristics that make up the different behaviors, events that shape the formation and personality of people in groups are used. With four generations occupying the same space, Baby Boomer, Generations X, Y, Z and Alpha, the segmentation opportunity grows so that communication is directed as a way to more effectively achieve environmental awareness, pre-establishing cultural standards, social, financial, professional, etc Thus, it is necessary to identify actions aimed at new behavioral paradigms, as a means of more effective and adequate access for these audiences, with a focus on the constant search for better results and the possibility of improving the environment.Con el advenimiento de la globalización, las herramientas de acceso a la comunicación crecen cada día. Casi al instante, la búsqueda continúa mejorando el uso de las tecnologías de búsqueda modernas, haciendo que la información sea más rápida y asertiva en la búsqueda incansable de la calificación de estas audiencias. Así, como forma de entender las características que conforman los diferentes comportamientos, se utilizan eventos que conforman la formación y personalidad de las personas en grupos.Con cuatro generaciones ocupando el mismo espacio, Baby Boomer, Generations X, Y, Z y Alpha, la oportunidad de segmentación crece para que la comunicación se dirija como una forma de lograr de manera más efectiva la conciencia ambiental, preestando estándares culturales, sociales, financieros, profesionales, etc.Así, es necesario identificar acciones dirigidas a nuevos paradigmas conductuales, como medio de acceso más efectivo y adecuado para estos públicos, con un enfoque en la búsqueda constante de mejores resultados y la posibilidad de mejorar el entorno.Com o advento da globalização, as ferramentas de acesso a comunicação crescem a cada dia. De maneira quase instantânea, a busca continua pelo aperfeiçoamento da utilização das modernas tecnologias de busca, faz com que a informação seja cada vez mais rápida e assertiva na incansável busca pela qualificação destes públicos. Assim, como forma de entender as características que compõem os diversos comportamentos, são utilizados acontecimentos que moldam a formação e a personalidade das pessoas em grupos. Com quatro gerações ocupando o mesmo espaço, Baby Boomer, Gerações X;Y,Z e Alpha, cresce a oportunidade de segmentação para que a comunicação seja direcionada como forma de atingir de maneira mais efetiva, a conscientização ambiental, pré-estabelecendo padrões culturais, sociais, financeiros, profissionais etc. Desta forma, é necessária a identificação de ações voltadas para os novos paradigmas comportamentais, como forma de acessos mais efetivos e adequados para estes públicos, com foco na busca constante de melhores resultados e possibilidade de melhorias para o meio ambiente. &nbsp

Angiotensin Converting Enzyme Regulates Cell Proliferation and Migration

Background The angiotensin-I converting enzyme (ACE) plays a central role in the renin-angiotensin system, acting by converting the hormone angiotensin-I to the active peptide angiotensin-II (Ang-II). More recently, ACE was shown to act as a receptor for Ang-II, and its expression level was demonstrated to be higher in melanoma cells compared to their normal counterparts. However, the function that ACE plays as an Ang-II receptor in melanoma cells has not been defined yet. Aim Therefore, our aim was to examine the role of ACE in tumor cell proliferation and migration. Results We found that upon binding to ACE, Ang-II internalizes with a faster onset compared to the binding of Ang-II to its classical AT1 receptor. We also found that the complex Ang-II/ACE translocates to the nucleus, through a clathrin-mediated process, triggering a transient nuclear Ca2+ signal. In silico studies revealed a possible interaction site between ACE and phospholipase C (PLC), and experimental results in CHO cells, demonstrated that the beta 3 isoform of PLC is the one involved in the Ca2+ signals induced by Ang-II/ACE interaction. Further studies in melanoma cells (TM-5) showed that Ang-II induced cell proliferation through ACE activation, an event that could be inhibited either by ACE inhibitor (Lisinopril) or by the silencing of ACE. In addition, we found that stimulation of ACE by Ang-II caused the melanoma cells to migrate, at least in part due to decreased vinculin expression, a focal adhesion structural protein. Conclusion ACE activation regulates melanoma cell proliferation and migration.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)INCT Nanocarbono - UFMG (Brazil)Univ Fed Minas Gerais, Dept Physiol & Biophys, Belo Horizonte, MG, BrazilUniv Fed Sao Joao del Rei, Dept Nat Sci, Sao Joao Del Rei, MG, BrazilUniv Fed Ceara, Dept Phys, Fortaleza, CE, BrazilUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, SP, BrazilUniv Fed Minas Gerais, Dept Phys, Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Dept Morphol, Belo Horizonte, MG, BrazilDepartment of Biophysics, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, BrazilWeb of Scienc

Características biométricas e descrição morfológica de frutos, sementes e plântulas de Combretum leprosum Mart.

O mofumbo (Combretum leprosum Mart.) é uma planta com uso na apicultura, medicina popular, forrageira, e com grande potencial na recuperação de áreas degradadas, principalmente por ser resistente a queimadas e cortes. Foram conduzidos estudos visando a caracterizar frutos, sementes, plântulas, mudas, germinação e emergência de Combretum leprosum. Foram realizadas medições de frutos e sementes, com paquímetro digital; massa de 1000 frutos e 1000 sementes, em balança semi-analítica; teor de umidade das sementes, pelo método de estufa a 105±3ºC por 24hs; 100 sementes foram colocadas para germinar em bandeja de germinação contendo fibra de coco para descrição da germinação e confecção das ilustrações. A unidade de dispersão é o fruto, seco, indeiscente, castanho, do tipo betulídio com uma semente por fruto. A semente é monospérmica com 4 estrias longitudinais; peso de mil sementes é 117,3g com 11,5% de umidade. A germinação é hipógea criptocotiledonar, com cotilédones de armazenamento. Protófilos e metáfilos apresentam diferenças na filotaxia. Os resultados obtidos podem ser empregados na identificação taxonômica e em trabalhos de tecnologia de sementes

Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up

OBJECTIVE: The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. INTRODUCTION: The human β-herpesviruses, including cytomegalovirus and human herpesvirus 6, are ubiquitous among human populations. Active infections of human herpesvirus 6 and cytomegalovirus are common after liver transplantation, possibly induced and facilitated by allograft rejection and immunosuppressive therapy. Both viruses affect the success of the transplant procedure. METHODS: Thirty patients submitted to liver transplant at the Liver Transplant Unit, at the Gastro Center, State University of Campinas, SP, Brazil, were studied prospectively from six months to one year, nested-polymerase chain reaction for cytomegalovirus and human herpesvirus 6 DNA detections. Two or more consecutive positive nested-polymerase chain reaction were considered indicative of active infection. RESULTS: Active infection by cytomegalovirus was detected in 13/30 (43.3%) patients, median time to first cytomegalovirus detection was 29 days after transplantation (range: 0-99 days). Active infection by human herpesvirus 6 was detected in 12/30 (40%) patients, median time to first human herpesvirus 6 detection was 23.5 days after transplantation (range: 0-273 days). The time-related appearance of each virus was not statistically different (p = 0.49). Rejection of the transplanted liver was observed in 16.7% (5/30) of the patients. The present analysis showed that human herpesvirus 6 and/or cytomegalovirus active infections were frequent in liver transplant recipients at our center. CONCLUSIONS: Few patients remain free of betaherpesviruses after liver transplantation. Most patients presenting active infection with more than one virus were infected sequentially and not concurrently. Nested-polymerase chain reaction can be considered of limited value for clinically monitoring cytomegalovirus and human herpesvirus 6

Simultaneous Monitoring Of Cmv And Human Herpesvirus 6 Infections And Diseases In Liver Transplant Patients: One-year Follow-up.

The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. The human β-herpesviruses, including cytomegalovirus and human herpesvirus 6, are ubiquitous among human populations. Active infections of human herpesvirus 6 and cytomegalovirus are common after liver transplantation, possibly induced and facilitated by allograft rejection and immunosuppressive therapy. Both viruses affect the success of the transplant procedure. Thirty patients submitted to liver transplant at the Liver Transplant Unit, at the Gastro Center, State University of Campinas, SP, Brazil, were studied prospectively from six months to one year, nested-polymerase chain reaction for cytomegalovirus and human herpesvirus 6 DNA detections. Two or more consecutive positive nested-polymerase chain reaction were considered indicative of active infection. Active infection by cytomegalovirus was detected in 13/30 (43.3%) patients, median time to first cytomegalovirus detection was 29 days after transplantation (range: 0-99 days). Active infection by human herpesvirus 6 was detected in 12/30 (40%) patients, median time to first human herpesvirus 6 detection was 23.5 days after transplantation (range: 0-273 days). The time-related appearance of each virus was not statistically different (p = 0.49). Rejection of the transplanted liver was observed in 16.7% (5/30) of the patients. The present analysis showed that human herpesvirus 6 and/or cytomegalovirus active infections were frequent in liver transplant recipients at our center. Few patients remain free of betaherpesviruses after liver transplantation. Most patients presenting active infection with more than one virus were infected sequentially and not concurrently. Nested-polymerase chain reaction can be considered of limited value for clinically monitoring cytomegalovirus and human herpesvirus 6.66949-5

Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses

Plant dehydrins (DNHs) belong to the LEA (Late Embryogenesis Abundant) protein family and are involved in responses to multiple abiotic stresses. DHNs are classified into five subclasses according to the organization of three conserved motifs (K-; Y-; and S-segments). In the present study, the DHN protein family was characterized by molecular phylogeny, exon/intron organization, protein structure, and tissue-specificity expression in eight Fabaceae species. We identified 20 DHN genes, encompassing three (YnSKn, SKn, and Kn) subclasses sharing similar gene organization and protein structure. Two additional low conserved DHN Φ-segments specific to the legume SKn-type of proteins were also found. The in silico expression patterns of DHN genes in four legume species (Arachis duranensis, A. ipaënsis, Glycine max, and Medicago truncatula) revealed that their tissue-specific regulation is associated with the presence or absence of the Y-segment. Indeed, DHN genes containing a Y-segment are mainly expressed in seeds, whereas those without the Y-segment are ubiquitously expressed. Further qRT-PCR analysis revealed that, amongst stress responsive dehydrins, a SKn-type DHN gene from A. duranensis (AdDHN1) showed opposite response to biotic and abiotic stress with a positive regulation under water deficit and negative regulation upon nematode infection. Furthermore, transgenic Arabidopsis lines overexpressing (OE) AdDHN1 displayed improved tolerance to multiple abiotic stresses (freezing and drought) but increased susceptibility to the biotrophic root-knot nematode (RKN) Meloidogyne incognita. This contradictory role of AdDHN1 in responses to abiotic and biotic stresses was further investigated by qRT-PCR analysis of transgenic plants using a set of stress-responsive genes involved in the abscisic acid (ABA) and jasmonic acid (JA) signaling pathways and suggested an involvement of DHN overexpression in these stress-signaling pathways

CERCAMENTO DO PARQUE FARROUPILHA - PORTO ALEGRE/RS – REDENÇÃO OU PRISÃO?

O fenômeno paisagístico é um processo cultural que se estabelece a partir da interação entre sujeitos e espaço percebido. A paisagem é um fenômeno vivo, contínuo, que expressa diferentes tempos, materialidades e arranjos sociais. É a metonímia de um território. Tendo isso em vista, um grupo de pesquisa interdisciplinar buscou experimentar diferentes ferramentas teórico-metodológicas ao realizar o exercício de intervir em uma determinada paisagem urbana, e assim, estimular o debate sobre as controvérsias que lhe são inerentes. A problemática escolhida foi a proposta de cercamento do Parque da Redenção, oficialmente denominado Parque Farroupilha, discutida na Câmara de Vereadores de Porto Alegre (RS) e na mídia local desde a década de 1990. Em uma área próxima ao lago Guaíba e ao Centro Histórico da cidade, os 40 hectares de área verde com diversos ambientes são um importante marco da paisagem de Porto Alegre. Caso fossem cercados, quais os aspectos da paisagem e outros que se agregam a ela que estariam sendo alterados? Como realizar uma intervenção para sensibilizar a população sobre as implicações do cercamento, ou não, do parque? Desde essas questões realizou-se o exercício, em 08 de novembro de 2014, cujos registros orientaram o debate e a produção deste texto e de um vídeo. Neste texto, procuramos inicialmente realizar um diagnóstico a paisagem estudada e a tensão evocada pela proposta de alterá-la mediante a instalação de uma cerca. A seguir relatamos uma intervenção realizada no local pelo grupo, como ferramenta metodológica para estimular diferentes reações e assim registrar as sensações e opiniões da população frente à alteração provocada naquela paisagem. Posteriormente, apresentamos reflexões sobre diferentes aspectos e implicações que o fechamento do parque poderá acarretar

Health-related quality of life in patients with type 1 diabetes mellitus in the different geographical regions of Brazil : data from the Brazilian Type 1 Diabetes Study Group

Background: In type 1 diabetes mellitus (T1DM) management, enhancing health-related quality of life (HRQoL) is as important as good metabolic control and prevention of secondary complications. This study aims to evaluate possible regional differences in HRQoL, demographic features and clinical characteristics of patients with T1DM in Brazil, a country of continental proportions, as well as investigate which variables could influence the HRQoL of these individuals and contribute to these regional disparities. Methods: This was a retrospective, cross-sectional, multicenter study performed by the Brazilian Type 1 Diabetes Study Group (BrazDiab1SG), by analyzing EuroQol scores from 3005 participants with T1DM, in 28 public clinics, among all geographical regions of Brazil. Data on demography, economic status, chronic complications, glycemic control and lipid profile were also collected. Results: We have found that the North-Northeast region presents a higher index in the assessment of the overall health status (EQ-VAS) compared to the Southeast (74.6 ± 30 and 70.4 ± 19, respectively; p < 0.05). In addition, North- Northeast presented a lower frequency of self-reported anxiety-depression compared to all regions of the country (North-Northeast: 1.53 ± 0.6; Southeast: 1.65 ± 0.7; South: 1.72 ± 0.7; Midwest: 1.67 ± 0.7; p < 0.05). These findings could not be entirely explained by the HbA1c levels or the other variables examined. Conclusions: Our study points to the existence of additional factors not yet evaluated that could be determinant in the HRQoL of people with T1DM and contribute to these regional disparities

Geographic range extension of Elachistocleis corumbaensis Piva, Caramaschi &amp;amp; Albuquerque, 2017 (Anura, Microhylidae) with new records in ecotonal zones in the state of Mato Grosso, Brazil

Elachistocleis corumbaensis was documented in 2017 in the central region of the Brazilian Pantanal and no other record of the species has since been reported. In this study, we report on the extension of the geographic range of E. corumbaensis based on 4 new records found in the riparian forests of the Paraguay River, in the state of Mato Grosso. Of these, 1 specimen was collected in a protected area in the Pantanal. We also report on the presence of this species in the ecotones between the Pantanal, Cerrado, and Amazonia, which suggests that E. corumbaensis is associated with seasonally flooded forests but more widely distributed in western Brazil than previously reported