In situ analysis of pH gradients in mosquito larvae using non-invasive, self-referencing, pH-sensitive microelectrodes

The alkaline environment, pH approximately 11, in the anterior midgut lumen of mosquito larvae is essential for normal nutrition and development. The mechanism of alkalization is, however, unknown. Although evidence from immunohistochemistry, electron microscopy and electrophysiology suggests that a V-ATPase is present in the basal membranes of the epithelial cells, its physiological role in the alkalization process has not been demonstrated. To investigate a possible role of the V-ATPase in lumen alkalization, pH gradients emanating from the hemolymph side of the midgut in semi-intact mosquito larvae were measured using non-invasive, self-referencing, ion-selective microelectrodes (SERIS). Large H+ concentration gradients, with highest concentrations close to the basal membrane (outward [H+] gradients), were found in the anterior midgut, whereas much smaller gradients, with concentrations lowest close to this membrane (inward [H+] gradients), were found in the gastric caeca and posterior midgut. Similar region-specific pH gradients, with consistent anterior-to-posterior profiles, were observed in individuals of two Aedes species, Aedes aegypti from semi-tropical Florida and Aedes canadensis from north-temperate Massachusetts. The gradients remained in a steady state for up to 6 h, the maximum duration of the recordings. Bafilomycin A1 (10(-5), 10(-7 )mol x l(-1)) on the hemolymph side greatly diminished the [H+] gradients in the anterior midgut but had no effect on the gradients in the gastric caecum and posterior midgut. These physiological data are consistent with the previous findings noted above. Together, they support the hypothesis that a basal, electrogenic H+ V-ATPase energizes luminal alkalization in the anterior midgut of larval mosquitoes

Efficient sorting of free electron orbital angular momentum

We propose a method for sorting electrons by orbital angular momentum (OAM). Several methods now exist to prepare electron wavefunctions in OAM states, but no technique has been developed for efficient, parallel measurement of pure and mixed electron OAM states. The proposed technique draws inspiration from the recent demonstration of the sorting of OAM through modal transformation. We show that the same transformation can be performed on electrons with electrostatic optical elements. Specifically, we show that a charged needle and an array of electrodes perform the transformation and phase correction necessary to sort OAM states. This device may enable the analysis of the spatial mode distribution of inelastically scattered electrons

Sigma-model soliton intersections from exceptional calibrations

A first-order `BPS' equation is obtained for 1/8 supersymmetric intersections of soliton-membranes (lumps) of supersymmetric (4+1)-dimensional massless sigma models, and a special non-singular solution is found that preserves 1/4 supersymmetry. For 4-dimensional hyper-K\"ahler target spaces ($HK_4$) the BPS equation is shown to be the low-energy limit of the equation for a Cayley-calibrated 4-surface in \bE^4\times HK_4. Similar first-order equations are found for stationary intersections of Q-lump-membranes of the massive sigma model, but now generic solutions preserve either 1/8 supersymmetry or no supersymmetry, depending on the time orientation.Comment: 21 pages. Version 3: Minor corrections and one further reference: version published in JHE

All-cause mortality and serious cardiovascular events in people with hip and knee osteoarthritis: a population based cohort study

BACKGROUND: Because individuals with osteoarthritis (OA) avoid physical activities that exacerbate symptoms, potentially increasing risk for cardiovascular disease (CVD) and death, we assessed the relationship between OA disability and these outcomes. METHODS: In a population cohort aged 55+ years with at least moderately severe symptomatic hip and/or knee OA, OA disability (Western Ontario McMaster Universities (WOMAC) OA scores; Health Assessment Questionnaire (HAQ) walking score; use of walking aids) and other covariates were assessed by questionnaire. Survey data were linked to health administrative data to determine the relationship between baseline OA symptom severity to all-cause mortality and occurrence of a composite CVD outcome (acute myocardial infarction, coronary revascularization, heart failure, stroke or transient ischemic attack) over a median follow-up of 13.2 and 9.2 years, respectively. RESULTS: Of 2156 participants, 1,236 (57.3%) died and 822 (38.1%) experienced a CVD outcome during follow-up. Higher (worse) baseline WOMAC function scores and walking disability were independently associated with a higher all-cause mortality (adjusted hazard ratio, aHR, per 10-point increase in WOMAC function score 1.04, 95% confidence interval, CI 1.01–1.07, p = 0.004; aHR per unit increase in HAQ walking score 1.30, 95% CI 1.22–1.39, p<0.001; and aHR for those using versus not using a walking aid 1.51, 95% CI 1.34–1.70, p<0.001). In survival analysis, censoring on death, risk of our composite CVD outcome was also significantly and independently associated with greater baseline walking disability ((aHR for use of a walking aid  = 1.27, 95% CI 1.10–1.47, p = 0.001; aHR per unit increase in HAQ walking score  = 1.17, 95% CI 1.08–1.27, p<0.001). CONCLUSIONS: Among individuals with hip and/or knee OA, severity of OA disability was associated with a significant increase in all-cause mortality and serious CVD events after controlling for multiple confounders. Research is needed to elucidate modifiable mechanisms

Spectroscopic evidence for the binary nature of AM CVn

We analysed archival spectroscopic data of AM CVn taken with the William Herschel Telescope in 1996. In the literature two orbital periods for AM CVn are proposed. A clear S-wave in the HeI 4471, 4387 and 4143 \AA lines is revealed when the spectra are folded on the 1029 s period. No signature of this S-wave is seen when folded on 1051 s. Doppler tomography of the line profiles shows a clear signature of the hotspot. Using this we can constrain the value of K_2 to lie between 210 and 280 km/s. Our work confirms the binary nature of AM CVn beyond any doubt, establishes 1028.73 s as the true orbital period and supports the interpretation of AM CVn as a permanent superhump system.Comment: Accepted by MNRA

Dark matter astrometry: accuracy of subhalo positions for the measurement of self-interaction cross-sections

Direct evidence for the existence of dark matter and measurements of its interaction cross-section have been provided by the physical offset between dark matter and intracluster gas in merging systems like the Bullet Cluster. Although a smaller signal, this effect is more abundant in minor mergers where infalling substructure dark matter and gas are segregated. In such low-mass systems the gravitational lensing signal comes primarily from weak lensing. A fundamental step in determining such an offset in substructure is the ability to accurately measure the positions of dark matter subpeaks. Using simulated Hubble Space Telescope observations, we make a first assessment of the precision and accuracy with which we can measure infalling groups using weak gravitational lensing. We demonstrate that using an existing and well-used mass reconstruction algorithm can measure the positions of 1.5 × 1013 M⊙ substructures that have parent haloes 10 times more massive with a bias of less than 0.3 arcsec. In this regime, our analysis suggests the precision is sufficient to detect (at 3σ statistical significance) the expected mean offset between dark matter and baryonic gas in infalling groups from a sample of ∼50 massive clusters

Tachyon condensation on brane sphalerons

We consider a sphaleron solution in field theory that provides a toy model for unstable D-branes of string theory. We investigate the tachyon condensation on a Dp-brane. The localized modes, including a tachyon, arise in the spectrum of a sphaleron solution of a \phi^4 field theory on M^{p+1}\times S^1. We use these modes to find a multiscalar tachyon potential living on the sphaleron world-volume. A complete cancelation between brane tension and the minimum of the tachyon potential is found as the size of the circle becomes small.Comment: To appear in JHEP, 13 pages, 2 eps figures, minor changes and references adde

Vector Bundle Moduli and Small Instanton Transitions

We give the general presciption for calculating the moduli of irreducible, stable SU(n) holomorphic vector bundles with positive spectral covers over elliptically fibered Calabi-Yau threefolds. Explicit results are presented for Hirzebruch base surfaces B=F_r. The transition moduli that are produced by chirality changing small instanton phase transitions are defined and specifically enumerated. The origin of these moduli, as the deformations of the spectral cover restricted to the ``lift'' of the horizontal curve of the M5-brane, is discussed. We present an alternative description of the transition moduli as the sections of rank n holomorphic vector bundles over the M5-brane curve and give explicit examples. Vector bundle moduli appear as gauge singlet scalar fields in the effective low-energy actions of heterotic superstrings and heterotic M-theory.Comment: 52 pages, LATEX, corrected typo

Vector Bundle Moduli Superpotentials in Heterotic Superstrings and M-Theory

The non-perturbative superpotential generated by a heterotic superstring wrapped once around a genus-zero holomorphic curve is proportional to the Pfaffian involving the determinant of a Dirac operator on this curve. We show that the space of zero modes of this Dirac operator is the kernel of a linear mapping that is dependent on the associated vector bundle moduli. By explicitly computing the determinant of this map, one can deduce whether or not the dimension of the space of zero modes vanishes. It is shown that this information is sufficient to completely determine the Pfaffian and, hence, the non-perturbative superpotential as explicit holomorphic functions of the vector bundle moduli. This method is illustrated by a number of non-trivial examples.Comment: 81 pages, LaTeX, corrected typo

Decomposition and nutrient release of leguminous plants in coffee agroforestry systems.

Leguminous plants used as green manure are an important nutrient source for coffee plantations, especially for soils with low nutrient levels. Field experiments were conducted in the Zona da Mata of Minas Gerais State, Brazil to evaluate the decomposition and nutrient release rates of four leguminous species used as green manures (Arachis pintoi, Calopogonium mucunoides, Stizolobium aterrimum and Stylosanthes guianensis) in a coffee agroforestry system under two different climate conditions. The initial N contents in plant residues varied from 25.7 to 37.0 g kg-1 and P from 2.4 to 3.0 g kg-1. The lignin/N, lignin/polyphenol and(lignin+polyphenol)/N ratios were low in all residues studied. Mass loss rates were highest in the first 15 days, when 25 % of the residues were decomposed. From 15 to 30 days, the decomposition rate decreased on both farms. On the farm in Pedra Dourada (PD), the decomposition constant k increased in the order C. mucunoides < S. aterrimum < S. guianensis < A. pintoi. On the farm in Araponga (ARA), there was no difference in the decomposition rate among leguminous plants. The N release rates varied from 0.0036 to 0.0096 d-1. Around 32 % of the total N content in the plant material was released in the first 15 days. In ARA, the N concentration in the S. aterrimum residues was always significantly higher than in the other residues. At the end of 360 days, the N released was 78 % in ARA and 89 % in PD of the initial content. Phosphorus was the most rapidly released nutrient (k values from 0.0165 to 0.0394 d-1). Residue decomposition and nutrient release did not correlate with initial residue chemistry and biochemistry, but differences in climatic conditions between the two study sites modified the decomposition rate constants