Performance of heterogeneous earthfill dams under earthquakes: optimal location of the impervious core

Earthfill dams are man-made geostructures which may be especially damaged by seismic loadings, because the soil skeleton they are made of suffers remarkable modifications in its mechanical properties, as well as changes of pore water pressure and flow of this water inside their pores, when subjected to vibrations. The most extreme situation is the dam failure due to soil liquefaction. Coupled finite element numerical codes are a useful tool to assess the safety of these dams. In this paper the application of a fully coupled numerical model, previously developed and validated by the authors, to a set of theoretical cross sections of earthfill dams with impervious core, is presented. All these dams are same height and have the same volume of impervious material at the core. The influence of the core location inside the dam on its response against seismic loading is numerically explored. The dams are designed as strictly stable under static loads. As a result of this research, a design recommendation on the location of the impervious core is obtained for this type of earth dams, on the basis of the criteria of minor liquefaction risk, minor soil degradation during the earthquake and minor crest settlement

Electrical conductivity measurement: a new technique to detect iatrogenic initial pedicle perforation

Abstract Pedicle screw fixation has achieved significant popularity amongst spinal surgeons for both single and multi-level spinal fusion. Misplacement and pedicle cortical violation occurs in over 20% of screw placement and can result in potential complications such as dysthesia, paraparesis or paraplegia. There have been many advances in techniques available for navigating through the pedicle; however, these techniques are not without drawbacks. A new electrical conductivity-measuring device, previously evaluated on the porcine model to detect the pedicle violation, was evaluated amongst nine European Hospitals to be used in conjunction with the methods currently used in that centre. This new device is based on two original principles; the device is integrated in the drilling or screwing tool. The technology allows real-time detection of perforation through two independent parameters, impedance variation and evoked muscle contractions. Data was collected twofold. Initially, the surgeon was given the device and a comparison was made between the devices ability to detect a breech and the surgeon's ability to detect one using his traditional methods of pedicle preparation. In the second module of the study, the surgeon was limited to using the electrical conductivity detection device as their sole guide to detect pedicle breaches. A comparison was made between the detection ability of the device and the other detection possibilities. Post-operative fine cut CT scanning was used to detect the pedicle breaches. Overall, the 11 trial surgeons performed a total of 521 pedicle drillings on 97 patients. Initially there were 147 drillings with 23 breaches detected. The detection rate of these breaches were 22/23 for the device compared to 10/23 by the surgeon. Over both parts of the study 64 breaches (12.3%) were confirmed on post-operative CT imaging. The electrical conductivity detection device detected 63 of the 64 breaches (98.4%). There was one false negative and four false positives. This gives the device an overall sensitivity of 98% and specificity of 99% for detecting a pedicle breach. The negative predictive value was 99.8%, with a positive predictive value of 94%. No adverse event was noted with the use of the electrical conductivity device. Electrical conductivity monitoring may provide

The AMIGA sample of isolated galaxies: XIV. Disc breaks and interactions through ultra-deep optical imaging*

Context. In the standard cosmological model of galaxy evolution, mergers and interactions play a fundamental role in shaping galaxies. Galaxies that are currently isolated are thus interesting because they allow us to distinguish between internal and external processes that affect the galactic structure. However, current observational limits may obscure crucial information in the low-mass or low-brightness regime. Aims. We use optical imaging of a subsample of the AMIGA catalogue of isolated galaxies to explore the impact of different factors on the structure of these galaxies. In particular, we study the type of disc break as a function of the degree of isolation and the presence of interaction indicators such as tidal streams or plumes, which are only detectable in the ultra-low surface brightness regime. Methods. We present ultra-deep optical imaging in the r band of a sample of 25 low-redshift (z< 0.035) isolated galaxies. Through careful data processing and analysis techniques, the nominal surface brightness limits achieved are comparable to those to be obtained on the ten-year LSST coadds (μr,lim ≳ 29.5 mag arcsec-2 [3σ; 10'' × 10'']). We place special emphasis on preserving the low surface brightness features throughout the processing. Results. The extreme depth of our imaging allows us to study the interaction signatures of 20 galaxies since Galactic cirrus is a strong limiting factor in the characterisation of interactions for the remaining 5 of them. We detect previously unreported interaction features in 8 (40% ± 14%) galaxies in our sample. We identify 9 galaxies (36% ± 10%) with an exponential disc (Type I), 14 galaxies (56% ± 10%) with a down-bending (Type II) profile, and only 2 galaxies (8% ± 5%) with up-bending (Type III) profiles. Isolated galaxies have considerably more purely exponential discs and fewer up-bending surface brightness profiles than field or cluster galaxies. We find clear minor merger activity in some of the galaxies with single exponential or down-bending profiles, and both of the galaxies with up-bending profiles show signatures of a past interaction. Conclusions. We show the importance of ultra-deep optical imaging in revealing faint external features in galaxies that indicate a probable history of interaction. We confirm that up-bending profiles are likely produced by major mergers, while down-bending profiles are probably formed by a threshold in star formation. Unperturbed galaxies that slowly evolve with a low star formation rate could induce the high rate of Type I discs in isolated galaxies

Unveiling the environment and faint features of the isolated galaxy CIG 96 with deep optical and HI observations

Asymmetries in HI in galaxies are often caused by the interaction with close companions, making isolated galaxies an ideal framework to study secular evolution. The AMIGA project has demonstrated that isolated galaxies show the lowest level of asymmetry in their HI integrated profiles, yet some present significant asymmetries. CIG 96 (NGC 864) is a representative case reaching a 16% level. Our aim is to investigate the HI asymmetries of this spiral galaxy and what processes have triggered the star-forming regions observed in the XUV pseudoring. We performed deep optical observations at CAHA 1.23m, 2.2m and VST telescopes. We reach surface brightness (SB) limits of mu_2.2m = 27.5 mag arcsec-2 (Cous R) and mu_VST = 28.7mag arcsec-2 (r) that show the XUV pseudoring of the galaxy in detail. Additionally, a wavelet filtering of the HI data cube from our deep observations with E/VLA telescope allowed us to reach a column density of N_HI = 8.9x10^18 cm -2 (5sigma) (28"x28" beam), lower than in any isolated galaxy. We confirm that the HI extends farther than 4xr_25 in all directions. Furthermore, we detect for the first time two gaseous structures (10^6 Msol) in the outskirts. The g-r colour index image from 1.23m shows extremely blue colours in certain regions of the pseudoring where N_HI>8.5x10^20 cm-2 , whereas the rest show red colours. Galactic cirrus contaminate the field, setting an unavoidable detection limit at 28.5mag arcsec-2 (r). We detect no stellar link within 1degx1deg or gaseous link within 40'x40' between CIG 96 and any companion. The isolation criteria rule out interactions with other similar-sized galaxies for at least 2.7Gyr. Using existing stellar evolution models, the age of the pseudoring is estimated at 1Gyr or older. Undetected previously accreted companions and cold gas accretion remain as the main hypothesis to explain the optical pseudoring and HI features of CIG 96.Comment: 23 pages, 18 figures, 4 table

Molecular analysis of ex-vivo CD133+ GBM cells revealed a common invasive and angiogenic profile but different proliferative signatures among high grade gliomas

<p>Abstract</p> <p>Background</p> <p>Gliomas are the most common type of primary brain tumours, and in this group glioblastomas (GBMs) are the higher-grade gliomas with fast progression and unfortunate prognosis. Two major aspects of glioma biology that contributes to its awful prognosis are the formation of new blood vessels through the process of angiogenesis and the invasion of glioma cells. Despite of advances, two-year survival for GBM patients with optimal therapy is less than 30%. Even in those patients with low-grade gliomas, that imply a moderately good prognosis, treatment is almost never curative. Recent studies have demonstrated the existence of a small fraction of glioma cells with characteristics of neural stem cells which are able to grow <it>in vitro </it>forming neurospheres and that can be isolated <it>in vivo </it>using surface markers such as CD133. The aim of this study was to define the molecular signature of GBM cells expressing CD133 in comparison with non expressing CD133 cells. This molecular classification could lead to the finding of new potential therapeutic targets for the rationale treatment of high grade GBM.</p> <p>Methods</p> <p>Eight fresh, primary and non cultured GBMs were used in order to study the gene expression signatures from its CD133 positive and negative populations isolated by FACS-sorting. Dataset was generated with Affymetrix U133 Plus 2 arrays and analysed using the software of the Affymetrix Expression Console. In addition, genomic analysis of these tumours was carried out by CGH arrays, FISH studies and MLPA;</p> <p>Results</p> <p>Gene expression analysis of CD133+ vs. CD133- cell population from each tumour showed that CD133+ cells presented common characteristics in all glioblastoma samples (up-regulation of genes involved in angiogenesis, permeability and down-regulation of genes implicated in cell assembly, neural cell organization and neurological disorders). Furthermore, unsupervised clustering of gene expression led us to distinguish between two groups of samples: those discriminated by tumour location and, the most importantly, the group discriminated by their proliferative potential;</p> <p>Conclusions</p> <p>Primary glioblastomas could be sub-classified according to the properties of their CD133+ cells. The molecular characterization of these potential stem cell populations could be critical to find new therapeutic targets and to develop an effective therapy for these tumours with very dismal prognosis.</p

Comprehensive analysis via exome sequencing uncovers genetic etiology in autosomal recessive nonsyndromic deafness in a large multiethnic cohort

Purpose:Autosomal recessive nonsyndromic deafness (ARNSD) is characterized by a high degree of genetic heterogeneity, with reported mutations in 58 different genes. This study was designed to detect deafness-causing variants in a multiethnic cohort with ARNSD by using whole-exome sequencing (WES).Methods:After excluding mutations in the most common gene, GJB2, we performed WES in 160 multiplex families with ARNSD from Turkey, Iran, Mexico, Ecuador, and Puerto Rico to screen for mutations in all known ARNSD genes.Results:We detected ARNSD-causing variants in 90 (56) families, 54 of which had not been previously reported. Identified mutations were located in 31 known ARNSD genes. The most common genes with mutations were MYO15A (13), MYO7A (11), SLC26A4 (10), TMPRSS3 (9), TMC1 (8), ILDR1 (6), and CDH23 (4). Nine mutations were detected in multiple families with shared haplotypes, suggesting founder effects.Conclusion:We report on a large multiethnic cohort with ARNSD in which comprehensive analysis of all known ARNSD genes identifies causative DNA variants in 56 of the families. In the remaining families, WES allows us to search for causative variants in novel genes, thus improving our ability to explain the underlying etiology in more families.Genet Med 18 4, 364-371. © American College of Medical Genetics and Genomics

The AMIGA sample of isolated galaxies

Context. In the standard cosmological model of galaxy evolution, mergers and interactions play a fundamental role in shaping galaxies. Galaxies that are currently isolated are thus interesting because they allow us to distinguish between internal and external processes that affect the galactic structure. However, current observational limits may obscure crucial information in the low-mass or low-brightness regime. Aims. We use optical imaging of a subsample of the AMIGA catalogue of isolated galaxies to explore the impact of different factors on the structure of these galaxies. In particular, we study the type of disc break as a function of the degree of isolation and the presence of interaction indicators such as tidal streams or plumes, which are only detectable in the ultra-low surface brightness regime. Methods. We present ultra-deep optical imaging in the r band of a sample of 25 low-redshift (z < 0.035) isolated galaxies. Through careful data processing and analysis techniques, the nominal surface brightness limits achieved are comparable to those to be obtained on the ten-year LSST coadds (μr,lim ≳ 29.5 mag arcsec−2 [3σ; 10″ × 10″]). We place special emphasis on preserving the low surface brightness features throughout the processing. Results. The extreme depth of our imaging allows us to study the interaction signatures of 20 galaxies since Galactic cirrus is a strong limiting factor in the characterisation of interactions for the remaining 5 of them. We detect previously unreported interaction features in 8 (40% ± 14%) galaxies in our sample. We identify 9 galaxies (36% ± 10%) with an exponential disc (Type I), 14 galaxies (56% ± 10%) with a down-bending (Type II) profile, and only 2 galaxies (8% ± 5%) with up-bending (Type III) profiles. Isolated galaxies have considerably more purely exponential discs and fewer up-bending surface brightness profiles than field or cluster galaxies. We find clear minor merger activity in some of the galaxies with single exponential or down-bending profiles, and both of the galaxies with up-bending profiles show signatures of a past interaction. Conclusions. We show the importance of ultra-deep optical imaging in revealing faint external features in galaxies that indicate a probable history of interaction. We confirm that up-bending profiles are likely produced by major mergers, while down-bending profiles are probably formed by a threshold in star formation. Unperturbed galaxies that slowly evolve with a low star formation rate could induce the high rate of Type I discs in isolated galaxies