Interaction of Hawking radiation with static sources in deSitter and Schwarzschild-deSitter spacetimes

We study and look for similarities between the response rates $R^{\rm dS}(a_0, \Lambda)$ and $R^{\rm SdS}(a_0, \Lambda, M)$ of a static scalar source with constant proper acceleration $a_0$ interacting with a massless, conformally coupled Klein-Gordon field in (i) deSitter spacetime, in the Euclidean vacuum, which describes a thermal flux of radiation emanating from the deSitter cosmological horizon, and in (ii) Schwarzschild-deSitter spacetime, in the Gibbons-Hawking vacuum, which describes thermal fluxes of radiation emanating from both the hole and the cosmological horizons, respectively, where $\Lambda$ is the cosmological constant and $M$ is the black hole mass. After performing the field quantization in each of the above spacetimes, we obtain the response rates at the tree level in terms of an infinite sum of zero-energy field modes possessing all possible angular momentum quantum numbers. In the case of deSitter spacetime, this formula is worked out and a closed, analytical form is obtained. In the case of Schwarzschild-deSitter spacetime such a closed formula could not be obtained, and a numerical analysis is performed. We conclude, in particular, that $R^{\rm dS}(a_0, \Lambda)$ and $R^{\rm SdS}(a_0, \Lambda, M)$ do not coincide in general, but tend to each other when $\Lambda \to 0$ or $a_0 \to \infty$. Our results are also contrasted and shown to agree (in the proper limits) with related ones in the literature.Comment: ReVTeX4 file, 9 pages, 5 figure

Do static sources respond to massive scalar particles from the Hawking radiation as uniformly accelerated ones do in the inertial vacuum?

We revisit the recently found equivalence for the response of a static scalar source interacting with a {\em massless} Klein-Gordon field when the source is (i) static in Schwarzschild spacetime, in the Unruh vacuum associated with the Hawking radiation and (ii) uniformly accelerated in Minkowski spacetime, in the inertial vacuum, provided that the source's proper acceleration is the same in both cases. It is shown that this equivalence is broken when the massless Klein-Gordon field is replaced by a {\em massive} one.Comment: 4 pages, 2 figure

Bacteriological conversion in twenty urinary tuberculosis patients treated with ofloxacin, rifampin and isoniazid: a 10-year follow-up study

Twenty patients withuri nary tuberculosis were treated withofloxac in (200 mg/day, 6 months), rifampin (600 mg/day, 3 months) and isoniazid (300 mg/day, 3 months) between 1989 and 1990. All patients were new cases, diagnosed by observation and/or isolation of Mycobacterium tuberculosis in one of the three morning urine samples. Bacteriological culture conversion (negativization) was assessed as a clinical guide of efficacy, comparing it, as the only parameter, against a control group (150 patients) withurina ry tuberculosis who received conventional therapy. Bacteriological follow-up studies were performed in bothgroups monthly for 6 months, then again 6 months later and then every year for 10 years after completion of treatment. In the 20 patients, the initial culture was positive with over 100 colonies per culture (>50%); the smear was positive in 45% of the patients (most were 2+). All strains were susceptible to rifampin, isoniazid and ofloxacin. Two patients discontinued treatment. Beginning withth e first monthof treatment, the bacteriological conversion was 100%, 89.5% and 100% in the remaining controls. In the control group, which received conventional treatment, the conversion was: 90%, 87%, 93% and 100% in the remaining controls. Treatment withofloxacin resulted in a bacteriological conversion similar to that following conventional treatment (p>0.05, Fisher’s exact test). After 10 years of patient follow-up, we conclude that ofloxacin, in combination withrifampin and isoniazid (bothfor 3 months only is effective against M. tuberculosis, providing satisfactory bacteriological and clinical efficacy

U-Pb zircon age of Ordovician magmatism in the Albera Massif (Eastern Pyrenees)

New geochronological data from the Albera Massif confirm the presence of an Early - Mid Ordovician igneous event (472 - 465Ma) recorded in the pre-Variscan rocks of the Pyrenees. This event resulted in the emplacement of a large granitic body in the lower part of the pre-Upper Ordovician metasedimentary succession and in the intrusion of a series of metric sized dykes in the middle and upper parts of it. The two types of igneous rocks were gneissified during subsequent Variscan deformation. The geochronological data confirm the occurrence of the gneiss as having derived from an Ordovician intrusive sheet, as in other Pyrenean massifs. The dykes are considered to be the subvolcanic equivalent of the intrusive sheet. The data also provide insight into the age of the metasedimentary series of the massif and enable us to correlate the dated rocks with other gneissic and subvolcanic bodies of the Variscan massifs of the Pyrenees and Iberia

U-Pb zircon age of Ordovician magmatism in the Albera Massif (Eastern Pyrenees)

New geochronological data from the Albera Massif confirm the presence of an Early – Mid Ordovician igneous event (472 - 465Ma) recorded in the pre-Variscan rocks of the Pyrenees. This event resulted in the emplacement of a large granitic body in the lower part of the pre-Upper Ordovician metasedimentary succession and in the intrusion of a series of metric sized dykes in the middle and upper parts of it. The two types of igneous rocks were gneissified during subsequent Variscan deformation. The geochronological data confirm the occurrence of the gneiss as having derived from an Ordovician intrusive sheet, as in other Pyrenean massifs. The dykes are considered to be the subvolcanic equivalent of the intrusive sheet. The data also provide insight into the age of the metasedimentary series of the massif and enable us to correlate the dated rocks with other gneissic and subvolcanic bodies of the Variscan massifs of the Pyrenees and Iberia

Low-energy sector quantization of a massless scalar field outside a Reissner-Nordstrom black hole and static sources

We quantize the low-energy sector of a massless scalar field in the Reissner-Nordstrom spacetime. This allows the analysis of processes involving soft scalar particles occurring outside charged black holes. In particular, we compute the response of a static scalar source interacting with Hawking radiation using the Unruh (and the Hartle-Hawking) vacuum. This response is compared with the one obtained when the source is uniformly accelerated in the usual vacuum of the Minkowski spacetime with the same proper acceleration. We show that both responses are in general different in opposition to the result obtained when the Reissner-Nordstrom black hole is replaced by a Schwarzschild one. The conceptual relevance of this result is commented.Comment: 12 pages (REVTEX), no figure

Multicentre evaluation of the Boehringer Mannheim / Hitachi 911 Analysis System

The analytical performance and practicability of the Boehringer Mannheim (BM)/mtaci 91 analysis system have been assessed in a multicentre evaluation, which involved six laboratories from European countries. Analytes commonly used in classical clinical chemistry were tested in a core programme, which mainlyfollowed lhe ECCLS guidelines. In addition, a satellite programme covered other analytes, such as proteins, drugs and urine analytes. In total, the study comprised more than 100 000 data items collected over a three-month period. The evaluation was supported with 'Computer Aided Evaluation' (CAEv) and telecommunications. Acceptance criteria for the results were established at the beginning ofthe study. Nearly all ofthe analytes met the imprecision limits.' within-run imprecision (as CVs) was 2l/ofor enzyme and substrate assays, l%for ISE methods and 5l/o for immunoassays; between-day imprecision was 3l/ofor enzyme and substrate assays, 2o//o for ISE methods and 10% for immunoassays

Newborn screening for medium-chain acyl-CoA dehydrogenase deficiency: regional experience and high incidence of carnitine deficiency

Background Medium-chain acyl-CoA dehydrogenase deficiency (MCADD) is the most common inherited defect in the mitochondrial fatty acid oxidation pathway, resulting in significant morbidity and mortality in undiagnosed patients. Newborn screening (NBS) has considerably improved MCADD outcome, but the risk of complication remains in some patients. The aim of this study was to evaluate the relationship between genotype, biochemical parameters and clinical data at diagnosis and during follow-up, in order to optimize monitoring of these patients. Methods We carried out a multicenter study in southwest Europe, of MCADD patients detected by NBS. Evaluated NBS data included free carnitine (C0) and the acylcarnitines C8, C10, C10:1 together with C8/C2 and C8/C10 ratios, clinical presentation parameters and genotype, in 45 patients. Follow-up data included C0 levels, duration of carnitine supplementation and occurrence of metabolic crises. Results C8/C2 ratio and C8 were the most accurate biomarkers of MCADD in NBS. We found a high number of patients homozygous for the prevalent c.985A > G mutation (75%). Moreover, in these patients C8, C8/C10 and C8/C2 were higher than in patients with other genotypes, while median value of C0 was significantly lower (23 μmol/L vs 36 μmol/L). The average follow-up period was 43 months. To keep carnitine levels within the normal range, carnitine supplementation was required in 82% of patients, and for a longer period in patients homozygotes for the c.985A>G mutation than in patients with other genotypes (average 31 vs 18 months). Even with treatment, median C0 levels remained lower in homozygous patients than in those with other genotypes (14 μmol/L vs 22 μmol/L). Two patients died and another three suffered a metabolic crisis, all of whom were homozygous for the c.985 A>G mutation. Conclusions Our data show a direct association between homozygosity for c.985A>G and lower carnitine values at diagnosis, and a higher dose of carnitine supplementation for maintenance within the normal range. This study contributes to a better understanding of the relationship between genotype and phenotype in newborn patients with MCADD detected through screening which could be useful in improving follow-up strategies and clinical outcome

Immunization with the MAEBL M2 domain protects against lethal Plasmodium yoelii infection

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOMalaria remains a world-threatening disease largely because of the lack of a long-lasting and fully effective vaccine. MAEBL is a type 1 transmembrane molecule with a chimeric cysteine-rich ectodomain homologous to regions of the Duffy binding-like erythrocyte binding protein and apical membrane antigen 1 (AMA1) antigens. Although MAEBL does not appear to be essential for the survival of blood-stage forms, ectodomains M1 and M2, homologous to AMA1, seem to be involved in parasite attachment to erythrocytes, especially M2. MAEBL is necessary for sporozoite infection of mosquito salivary glands and is expressed in liver stages. Here, the Plasmodium yoelii MAEBL-M2 domain was expressed in a prokaryotic vector. C57BL/6J mice were immunized with doses of P. yoelii recombinant protein rPyM2-MAEBL. High levels of antibodies, with balanced IgG1 and IgG2c subclasses, were achieved. rPyM2-MAEBL antisera were capable of recognizing the native antigen. Anti-MAEBL antibodies recognized different MAEBL fragments expressed in CHO cells, showing stronger IgM and IgG responses to the M2 domain and repeat region, respectively. After a challenge with P. yoelii YM (lethal strain)-infected erythrocytes (IE), up to 90% of the immunized animals survived and a reduction of parasitemia was observed. Moreover, splenocytes harvested from immunized animals proliferated in a dose-dependent manner in the presence of rPyM2-MAEBL. Protection was highly dependent on CD4(+), but not CD8(+), T cells toward Th1. rPyM2-MAEBL antisera were also able to significantly inhibit parasite development, as observed in ex vivo P. yoelii erythrocyte invasion assays. Collectively, these findings support the use of MAEBL as a vaccine candidate and open perspectives to understand the mechanisms involved in protection.Malaria remains a world-threatening disease largely because of the lack of a long-lasting and fully effective vaccine. MAEBL is a type 1 transmembrane molecule with a chimeric cysteine-rich ectodomain homologous to regions of the Duffy binding-like erythr831037813792FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOsem informaçãosem informaçã

Fucosylated Chondroitin Sulfate Inhibits Plasmodium Falciparum Cytoadhesion And Merozoite Invasion.

Sequestration of Plasmodium falciparum-infected erythrocytes (Pf-iEs) in the microvasculature of vital organs plays a key role in the pathogenesis of life-threatening malaria complications, such as cerebral malaria and malaria in pregnancy. This phenomenon is marked by the cytoadhesion of Pf-iEs to host receptors on the surfaces of endothelial cells, on noninfected erythrocytes, and in the placental trophoblast; therefore, these sites are potential targets for antiadhesion therapies. In this context, glycosaminoglycans (GAGs), including heparin, have shown the ability to inhibit Pf-iE cytoadherence and growth. Nevertheless, the use of heparin was discontinued due to serious side effects, such as bleeding. Other GAG-based therapies were hampered due to the potential risk of contamination with prions and viruses, as some GAGs are isolated from mammals. In this context, we investigated the effects and mechanism of action of fucosylated chondroitin sulfate (FucCS), a unique and highly sulfated GAG isolated from the sea cucumber, with respect to P. falciparum cytoadhesion and development. FucCS was effective in inhibiting the cytoadherence of Pf-iEs to human lung endothelial cells and placenta cryosections under static and flow conditions. Removal of the sulfated fucose branches of the FucCS structure virtually abolished the inhibitory effects of FucCS. Importantly, FucCS rapidly disrupted rosettes at high levels, and it was also able to block parasite development by interfering with merozoite invasion. Collectively, these findings highlight the potential of FucCS as a candidate for adjunct therapy against severe malaria.581862-7