Response kinetics in the complex chemotaxis signalling pathway of Rhodobacter sphaeroides

Chemotaxis is one of the best characterised signalling systems in biology. It is the mechanism by which bacteria move towards optimal environments and is implicated in biofilm formation, pathogenesis and symbiosis. The properties of the bacterial chemosensory response have been described in detail for the single chemosensory pathway of Escherichia coli. We have characterised the properties of the chemosensory response of Rhodobacter sphaeroides, an -proteobacterium with multiple chemotaxis pathways, under two growth conditions allowing the effects of protein expression levels and cell architecture to be investigated. Using tethered cell assays we measured the responses of the system to step changes in concentration of the attractant propionate and show that, independently of the growth conditions, R. sphaeroides is chemotactic over at least five orders of magnitude and has a sensing profile following Weber’s law. Mathematical modelling also shows that, like E. coli, R. sphaeroides is capable of showing Fold-Change Detection (FCD). Our results indicate that general features of bacterial chemotaxis such as the range and sensitivity of detection, adaptation times, adherence to Weber’s law and the presence of FCD may be integral features of chemotaxis systems in general, regardless of network complexity, protein expression levels and cellular architecture across different species

Experimental Space Shuttle Orbiter Studies to Acquire Data for Code and Flight Heating Model Validation

In an experimental study to obtain detailed heating data over the Space Shuttle Orbiter, CUBRC has completed an extensive matrix of experiments using three distinct models and two unique hypervelocity wind tunnel facilities. This detailed data will be employed to assess heating augmentation due to boundary layer transition on the Orbiter wing leading edge and wind side acreage with comparisons to computational methods and flight data obtained during the Orbiter Entry Boundary Layer Flight Experiment and HYTHIRM during STS-119 reentry. These comparisons will facilitate critical updates to be made to the engineering tools employed to make assessments about natural and tripped boundary layer transition during Orbiter reentry. To achieve the goals of this study data was obtained over a range of Mach numbers from 10 to 18, with flight scaled Reynolds numbers and model attitudes representing key points on the Orbiter reentry trajectory. The first of these studies were performed as an integral part of Return to Flight activities following the accident that occurred during the reentry of the Space Shuttle Columbia (STS-107) in February of 2003. This accident was caused by debris, which originated from the foam covering the external tank bipod fitting ramps, striking and damaging critical wing leading edge heating tiles that reside in the Orbiter bow shock/wing interaction region. During investigation of the accident aeroheating team members discovered that only a limited amount of experimental wing leading edge data existed in this critical peak heating area and a need arose to acquire a detailed dataset of heating in this region. This new dataset was acquired in three phases consisting of a risk mitigation phase employing a 1.8% scale Orbiter model with special temperature sensitive paint covering the wing leading edge, a 0.9% scale Orbiter model with high resolution thin-film instrumentation in the span direction, and the primary 1.8% scale Orbiter model with detailed thin-film resolution in both the span and chord direction in the area of peak heating. Additional objectives of this first study included: obtaining natural or tripped turbulent wing leading edge heating levels, assessing the effectiveness of protuberances and cavities placed at specified locations on the orbiter over a range of Mach numbers and Reynolds numbers to evaluate and compare to existing engineering and computational tools, obtaining cavity floor heating to aid in the verification of cavity heating correlations, acquiring control surface deflection heating data on both the main body flap and elevons, and obtain high speed schlieren videos of the interaction of the orbiter nose bow shock with the wing leading edge. To support these objectives, the stainless steel 1.8% scale orbiter model in addition to the sensors on the wing leading edge was instrumented down the windward centerline, over the wing acreage on the port side, and painted with temperature sensitive paint on the starboard side wing acreage. In all, the stainless steel 1.8% scale Orbiter model was instrumented with over three-hundred highly sensitive thin-film heating sensors, two-hundred of which were located in the wing leading edge shock interaction region. Further experimental studies will also be performed following the successful acquisition of flight data during the Orbiter Entry Boundary Layer Flight Experiment and HYTHIRM on STS-119 at specific data points simulating flight conditions and geometries. Additional instrumentation and a protuberance matching the layout present during the STS-119 boundary layer transition flight experiment were added with testing performed at Mach number and Reynolds number conditions simulating conditions experienced in flight. In addition to the experimental studies, CUBRC also performed a large amount of CFD analysis to confirm and validate not only the tunnel freestream conditions, but also 3D flows over the orbiter acreage, wing leading edge, and controlurfaces to assess data quality, shock interaction locations, and control surface separation regions. This analysis is a standard part of any experimental program at CUBRC, and this information was of key importance for post-test data quality analysis and understanding particular phenomena seen in the data. All work during this effort was sponsored and paid for by the NASA Space Shuttle Program Office at the Johnson Space Center in Houston, Texas

Robust wavebuoys for the marginal ice zone: Experiences from a large persistent array in the Beaufort Sea

An array of novel directional wavebuoys was designed and deployed into the Beaufort Sea ice cover in March 2014, as part of the Office of Naval Research Marginal Ice Zone experiment. The buoys were designed to drift with the ice throughout the year and monitor the expected breakup and retreat of the ice cover, forced by waves travelling into the ice from open water. Buoys were deployed from fast-and-light air-supported ice camps, based out of Sachs Harbour on Canada’s Banks Island, and drifted westwards with the sea ice over the course of spring, summer and autumn, as the ice melted, broke up and finally re-froze. The buoys transmitted heave, roll and pitch timeseries at 1 Hz sample frequency over the course of up to eight months, surviving both convergent ice dynamics and significant waves-in-ice events. Twelve of the 19 buoys survived until their batteries were finally exhausted during freeze-up in late October/November. Ice impact was found to have contaminated a significant proportion of the Kalman-filter-derived heave records, and these bad records were removed with reference to raw x/y/z accelerations. The quality of magnetometer-derived buoy headings at the very high magnetic field inclinations close to the magnetic pole was found to be generally acceptable, except in the case of four buoys which had probably suffered rough handling during transport to the ice. In general, these new buoys performed as expected, though vigilance as to the veracity of the output is required

Winter-to-summer transition of Arctic sea ice breakup and floe size distribution in the Beaufort Sea

Breakup of the near-continuous winter sea ice into discrete summer ice floes is an important transition that dictates the evolution and fate of the marginal ice zone (MIZ) of the Arctic Ocean. During the winter of 2014, more than 50 autonomous drifting buoys were deployed in four separate clusters on the sea ice in the Beaufort Sea, as part of the Office of Naval Research MIZ program. These systems measured the ocean-ice-atmosphere properties at their location whilst the sea ice parameters in the surrounding area of these buoy clusters were continuously monitored by satellite TerraSAR-X Synthetic Aperture Radar. This approach provided a unique Lagrangian view of the winter-to-summer transition of sea ice breakup and floe size distribution at each cluster between March and August. The results show the critical timings of a) temporary breakup of winter sea ice coinciding with strong wind events and b) spring breakup (during surface melt, melt ponding and drainage) leading to distinctive summer ice floes. Importantly our results suggest that summer sea ice floe distribution is potentially affected by the state of winter sea ice, including the composition and fracturing (caused by deformation events) of winter sea ice, and that substantial mid-summer breakup of sea ice floes is likely linked to the timing of thermodynamic melt of sea ice in the area. As the rate of deformation and thermodynamic melt of sea ice has been increasing in the MIZ in the Beaufort Sea, our results suggest that these elevated factors would promote faster and more enhanced breakup of sea ice, leading to a higher melt rate of sea ice and thus a more rapid advance of the summer MIZ

Simulation of sub-millimetre atmospheric spectra for characterizing potential ground-based remote sensing observations

The sub-millimetre is an understudied region of the Earth's atmospheric electromagnetic spectrum. Prior technological gaps and relatively high opacity due to the prevalence of rotational water vapour lines at these wavelengths have slowed progress from a ground-based remote sensing perspective; however, emerging superconducting detector technologies in the fields of astronomy offer the potential to address key atmospheric science challenges with new instrumental methods. A site study, with a focus on the polar regions, is performed to assess theoretical feasibility by simulating the downwelling clear-sky sub-millimetre spectrum from 30 mm (10 GHz) to 150 μm (2000 GHz) at six locations under annual mean, summer, winter, daytime, nighttime and low humidity conditions. Vertical profiles of temperature, pressure and 28 atmospheric gases are constructed by combining radiosonde, meteorological reanalysis, and atmospheric chemistry model data. The sensitivity of the simulated spectra to the choice of water vapour continuum model and spectroscopic line database is explored. For the atmospheric trace species hypobromous acid (HOBr), hydrogen bromide (HBr), perhydroxyl radical (HO2) and nitrous oxide (N2O) the emission lines producing the largest change in brightness temperature are identified. Signal strengths, centre frequencies, bandwidths, estimated minimum integration times and maximum receiver noise temperatures are determined for all cases. HOBr, HBr and HO2 produce brightness temperature peaks in the mK to K range, whereas the N2O peaks are in the K range. The optimal sub-millimetre remote sensing lines for the four species are shown to vary significantly between location and scenario, strengthening the case for future hyperspectral instruments that measure over a broad wavelength range. The techniques presented here provide a framework that can be applied to additional species of interest and taken forward to simulate retrievals and guide the design of future sub-millimetre instruments

Potential climatic transitions with profound impact on Europe

We discuss potential transitions of six climatic subsystems with large-scale impact on Europe, sometimes denoted as tipping elements. These are the ice sheets on Greenland and West Antarctica, the Atlantic thermohaline circulation, Arctic sea ice, Alpine glaciers and northern hemisphere stratospheric ozone. Each system is represented by co-authors actively publishing in the corresponding field. For each subsystem we summarize the mechanism of a potential transition in a warmer climate along with its impact on Europe and assess the likelihood for such a transition based on published scientific literature. As a summary, the ‘tipping’ potential for each system is provided as a function of global mean temperature increase which required some subjective interpretation of scientific facts by the authors and should be considered as a snapshot of our current understanding. <br/

NO PLIF Visualizations of the Orion Capsule in LENS-I

Planar laser-induced fluorescence (PLIF) of nitric oxide (NO) was used to visualize the interaction of reaction-control-system (RCS) jet flows in the wake of a hypersonic capsule reentry vehicle. The tests were performed at the Calspan University at Buffalo Research Center's (CUBRC) LENS-I reflected shock tunnel facility. This was the first application of PLIF to study RCS jets in a large-scale pulsed hypersonic facility. The LENS-I facility allowed RCS jet flows to be studied while varying the flow enthalpy, Reynolds number, angle of attack and jet configuration. The interaction of pitch and roll jets with the flowfield was investigated. Additionally, thin film sensors were used to monitor heat transfer on the surface of the model to detect any localized heating resulting from the firing of the RCS jets. Tests were conducted with the model held at angles of attack of 18deg and 22deg. The nominal Mach number in all tests was 8, while Reynolds number based on model diameter ranged from 2.2x10(exp 6) - 1.5x10(exp 7). Images were processed using the Virtual Diagnostics Interface (ViDI) system developed at NASA Langley Research Center to provide a three-dimensional display of the experimental data

Arctic sea ice and snow cover albedo variability and trends during the last three decades

The aim of the present study is to assess the full effect on the albedo of both sea ice extent decrease and snowline retreat in the Arctic during the last three decades. Averaged over the globe, the overall warming effect due to Arctic land and ocean albedo change corresponds to adding about 44% to the direct effect of human CO2 emissions during the same period. In fact, the area and thickness of Arctic sea ice have both been declining in this time frame. This has caused feedbacks affecting the whole global climate system. One such is albedo feedback of sea ice shrinking which was previously estimated (Pistone et al., 2014) to add about 25% to the direct warming effect of anthropogenic CO2 emissions. In this study, we demonstrate that the role of snowline retreat in albedo decrease is comparable to that of sea ice shrinking. To this aim, we estimate the radiative forcing (W/m2) due to snow and ice decrease during 34 years (1982–2015) from the analysis of changes of observed albedo based on the Clouds and the Earth's Radiant Energy System Energy Balanced And Filled (CERES EBAF) dataset, paired with sea ice and snow cover data from the US National Snow &amp; Ice Data Center (NSIDC)

Weddell Sea iceberg drift: Five years of observations

Since 1999, 52 icebergs have been tagged with GPS buoys in the Weddell Seato enable monitoring of their position. The chosen icebergs were of small tomedium size, with a few icebergs larger than 10 km associatedwith the calving of icebergs A38 and A43 from the Ronne Ice Shelf.The majority of icebergs were tagged off Neumayer Station (8E, 70S).It was found that smaller bergs with edges shorter than 200 m had the shortestlife cycle (< 0.5 yr). Iceberg and thus freshwater export out of theWeddell Sea was found to be highly variable. In one year the majority of buoysdeployed remained in the Weddell Sea, constituting about 40 % of the NCEP P-Efreshwater input, whereas in other years all of the tagged icebergs were exported.The observed drifts of icebergs and sea-ice showed a remarkably coherent motion.The analysis of an iceberg - sea-ice buoy array in the western Weddell Seaand an iceberg array in the eastern Weddell Sea showed a coherent sea-iceiceberg drift in sea-ice concentrations above 86 %. Dynamic kinematic parameter(DKP) during the course of coherent movement were low and deviations from the meancourse associated with the passage of low-pressure system. The length scale ofcoherent movement was estimated to be less than 250km; about half the value found forthe Arctic Ocean

Use of transcriptomic data for extending a model of the AppA/PpsR system in Rhodobacter sphaeroides

BackgroundPhotosynthetic (PS) gene expression in Rhodobacter sphaeroides is regulated in response to changes in light and redox conditions mainly by PrrB/A, FnrL and AppA/PpsR systems. The PrrB/A and FnrL systems activate the expression of them under anaerobic conditions while the AppA/PpsR system represses them under aerobic conditions. Recently, two mathematical models have been developed for the AppA/PpsR system and demonstrated how the interaction between AppA and PpsR could lead to a phenotype in which PS genes are repressed under semi-aerobic conditions. These models have also predicted that the transition from aerobic to anaerobic growth mode could occur via a bistable regime. However, they lack experimentally quantifiable inputs and outputs. Here, we extend one of them to include such quantities and combine all relevant micro-array data publically available for a PS gene of this bacterium and use that to parameterise the model. In addition, we hypothesise that the AppA/PpsR system alone might account for the observed trend of PS gene expression under semi-aerobic conditions. ResultsOur extended model of the AppA/PpsR system includes the biological input of atmospheric oxygen concentration and an output of photosynthetic gene expression. Following our hypothesis that the AppA/PpsR system alone is sufficient to describe the overall trend of PS gene expression we parameterise the model and suggest that the rate of AppA reduction in vivo should be faster than its oxidation. Also, we show that despite both the reduced and oxidised forms of PpsR binding to the PS gene promoters in vitro, binding of the oxidised form as a repressor alone is sufficient to reproduce the observed PS gene expression pattern. Finally, the combination of model parameters which fit the biological data well are broadly consistent with those which were previously determined to be required for the system to show (i) the repression of PS genes under semi-aerobic conditions, and (ii) bistability. ConclusionWe found that despite at least three pathways being involved in the regulation of photosynthetic genes, the AppA/PpsR system alone is capable of accounting for the observed trends in photosynthetic gene expression seen at different oxygen levels