Breast mammographic Density: stromal implications on breast cancer detection and therapy

Current evidences state clear that both normal development of breast tissue as well as its malignant progression need many-sided local and systemic communications between epithelial cells and stromal components. During development, the stroma, through remarkably regulated contextual signals, affects the fate of the different mammary cells regarding their specification and differentiation. Likewise, the stroma can generate tumour environments that facilitate the neoplastic growth of the breast carcinoma. Mammographic density has been described as a risk factor in the development of breast cancer and is ascribed to modifications in the composition of breast tissue, including both stromal and glandular compartments. Thus, stroma composition can dramatically affect the progression of breast cancer but also its early detection since it is mainly responsible for the differences in mammographic density among individuals. This review highlights both the pathological and biological evidences for a pivotal role of the breast stroma in mammographic density, with particular emphasis on dense and malignant stromas, their clinical meaning and potential therapeutic implications for breast cancer patients

Forming next-generation antibody–nanoparticle conjugates through the oriented installation of non-engineered antibody fragments

The successful development of targeted nanotherapeutics is contingent upon the conjugation of therapeutic nanoparticles to target-specific ligands, with particular emphasis being placed on antibody-based ligands. Thus, new methods that enable the covalent and precise installation of targeting antibodies to nanoparticle surfaces are greatly desired, especially those which do not rely on costly and time-consuming antibody engineering techniques. Herein we present a novel method for the highly controlled and oriented covalent conjugation of non-engineered antibody F(ab) fragments to PLGA–PEG nanoparticles using disulfide-selective pyridazinedione linkers and strain-promoted alkyne–azide click chemistry. Exemplification of this method with trastuzumab and cetuximab showed significant improvements in both conjugation efficiency and antigen binding capability, when compared to commonly employed strategies for antibody–nanoparticle construction. This new approach paves the way for the development of antibody-targeted nanomedicines with improved paratope availability, reproducibility and uniformity to enhance both biological activity and ease of manufacture

HABs in coastal upwelling systems: Insights from an exceptional red tide of the toxigenic dinoflagellate Alexandrium minutum

Alexandrium minutum blooms generally occur in semi-enclosed sites such as estuaries, harbours and lagoons, where enhanced stratification, restricted circulation and accumulation of resting cysts in the sediment set suitable habitat conditions for the proliferation of this paralytic shellfish poisoning toxigenic species. In the Galician Rías Baixas (NW Iberian Peninsula), according to weekly time-series between 1994 and 2020, blooms of A. minutum were recurrent in small, shallow estuarine bays inside the Rías de Vigo and Pontevedra, but rarely detected, and if so at low concentrations, out of these environments. However, from May to July 2018 it developed as usual in the small inner bays but then spread over both Rías (Vigo and Pontevedra) causing discoloured waters during one month and prolonged harvesting closures. Meteorological conditions during that period (rains / runoff higher than climatological averages, sustained temperature increment and oscillating wind pattern –i.e., series of upwelling-relaxation cycles), fostered optimal circumstances for the development of that extensive and massive proliferation: strong vertical stratification and the alternation of retention and dispersion processes. Simulations from a particle tracking model portrayed the observed bloom development phases: onset and development inside a small inner bay; transport within the surface layer, from these sites towards the interior parts of the Ría; and dispersion all over the embayment. Seedbeds with high concentrations of resting cysts were detected several months after the bloom, which may have favoured flourishment of A. minutum in the following two years, markedly in 2020. The present work contributes to the general understanding of the dynamics of harmful algal blooms (HABs), from which surveillance indicators of the state of marine ecosystems and their evolution can be derived. We hypothesize that the intensity and frequency of A. minutum proliferations in the Galician Rías could increase under projected climate trends.Postprint2,69

Bloom dynamics of an exceptional red tide of the toxigenic dinoflagellate

The toxic dinoflagellate Alexandrium minutum generally proliferates in semi-enclosed sites such as estuaries, harbours and lagoons, where stratification, restricted circulation and accumulation of resting cysts set suitable conditions for its development. In the Galician Rías (NW Iberian Peninsula), its blooms follow also this pattern. They are recurrent in small, shallow estuarine bays inside the Rías, but rarely detected, and if so in minor amount, out of these areas. However, a massive proliferation of A. minutum from June to July 2018 in the Rías Baixas (Vigo and Pontevedra) changed this picture. The bloom initiated in semi-enclosed waters, as previously described for this species, but thereafter spread to the whole embayments where persisted more than one month. It generated a noticeable red tide with disperse patches that became heavily concentrated inside the port of Vigo. During that period shellfish harvesting closures and paralytic shellfish toxins in certain marine invertebrates and fish were reported for the first time in Spain. Meteorological conditions (higher than usual rains/runoff, sustained temperature increment and oscillating wind pattern promoting a series of upwelling-relaxation cycles) fostered optimal circumstances for the outbreak of A. minutum: strong vertical stratification and the alternation of retention and dispersion processes. Simulations from a particle tracking model portrayed the observed bloom development phases: onset, transport within the surface layer towards the interior parts of the Ría of Vigo, and dispersion all over the embayment. High concentrations of resting cysts were detected several months after the bloom, which may have favoured flourish of A. minutum in the following years, markedly in 2020

Analysis of parcel-based image classification methods for monitoring the activities of the Land Bank of Galicia (Spain)

[EN] The abandonment of agricultural plots entails a low economic productivity of the land and a higher vulnerability to wildfires and degradation of affected areas. In this sense, the local government of Galicia is promoting new methodologies based on high-resolution images in order to classify the territory in basic and generic land uses. This procedure will be used to control the sustainable management of plots belonging to the Land Bank. This paper presents an application study for maintaining and updating land use/land cover geospatial databases using parcel-oriented classification. The test is performed over two geographic areas of Galicia, in the northwest of Spain. In this region, forest and shrublands in mountain environments are very heterogeneous with many private unproductive plots, some of which are in a high state of abandonment. The dataset is made of high spatial resolution multispectral imagery, cadastral cartography employed to define the image objects (plots), and field samples used to define evaluation and training samples. A set of descriptive features is computed quantifying different properties of the objects, i.e. spectral, texture, structural, and geometrical. Additionally, the effect on the classification and updating processes of the historical land use as a descriptive feature is tested. Three different classification methodologies are analyzed: linear discriminant analysis, decision trees, and support vector machine. The overall accuracies of the classifications obtained are always above 90 % and support vector machine method is proved to provide the best performance. Forest and shrublands areas are especially undefined, so the discrimination between these two classes is low. The results enable to conclude that the use of automatic parcel-oriented classification techniques for updating tasks of land use/land cover geospatial databases, is effective in the areas tested, particularly when broad and well defined classes are required.The authors appreciate the collaboration and support provided by Xunta de Galicia, Sociedade para o Desenvolvemento Comarcal de Galícia, and Banco de Terras de Galicia. The financial support provided by the Spanish Ministerio de Ciencia e Innovación in the framework of the projects CGL2010-19591/BTE and CGL2009-14220 is also acknowledged.Hermosilla, T.; Díaz Manso, J.; Ruiz Fernández, LÁ.; Recio Recio, JA.; Fernández-Sarría, A.; Ferradáns Nogueira, P. (2012). Analysis of parcel-based image classification methods for monitoring the activities of the Land Bank of Galicia (Spain). Applied Geomatics. 4(4):245-255. https://doi.org/10.1007/s12518-012-0087-zS24525544Arikan M (2004) Parcel-based crop mapping through multi-temporal masking classification of landsat 7 images in Karacabey, Turkey. Int Arch Photogramm Remote Sens Spat Inf Sci 35:1085–1090Balaguer A, Ruiz LA, Hermosilla T, Recio JA (2010) Definition of a comprehensive set of texture semivariogram features and their evaluation for object-oriented image classification. Comput Geosci 36(2):231–240Balaguer-Besser A, Hermosilla T, Recio JA, Ruiz LA (2011) Semivariogram calculation optimization for object-oriented image classification. Model Sci Educ Learn 4(7):91–104Blaschke T (2010) Object based image analysis for remote sensing. ISPRS J Photogramm 65(1):2–16Cohen Y, Shoshany M (2000) Integration of remote sensing, GIS and expert knowledge in national knowledge-based crop recognition in Mediterranean environment. Int Arch Photogramm Remote Sens 33(Part B7):280–286Congalton R (1991) A review of assessing the accuracy of classifications of remotely sensed data. Remote Sens Environ 37(1):35–46Dadhwal VK, Singh RP, Dutta S, Parihar JS (2002) Remote sensing based crop inventory: a review of Indian experience. Trop Ecol 43(1):107–122De Wit AJW, Clevers JGPW (2004) Efficiency and accuracy of per-field classification for operational crop mapping. Int J Remote Sens 25:4091–4112Del Frate F, Pacifici F, Solimini D (2008) Monitoring urban land cover in Rome, Italy, and its changes by single-polarization multitemporal SAR images. IEEE J Sel Top Appl Earth Obs Remote Sens 1:87–97Díaz-Manso JM, Ferradáns-Nogueira P (2011) Modelo de uso actual da terra. In: Cobelle-Rico EJ, Diaz-Manso JM, Crecente-Maseda R, Martínez-Rivas EM (eds) Mercado e Mobilidade de Terras en Galícia, 1st edn. Servizo de Publicacións e Intercambio Científico, Santiago de Compostela, Spain, pp 31–44Dupas CA (2000) SAR and LANDSAT TM image fusion for land cover classification in the Brazilian Atlantic Forest Domain. Int Arch Photogramm Remote Sens XXXIII(Part B1):96–103El Kady M, Mack CB (1992) Remote sensing for crop inventory of Egypt’s old agricultural lands. Int Arch Photogramm Remote Sens 29:176–185Everitt BS, Dunn G (2001) Applied multivariate data analysis, 2nd edn. Edward Arnold, LondonHaralick RM, Shanmugam K, Dinstein I (1973) Texture features for image classification. IEEE Transact Syst Man Cybern 3(6):610–622Hermosilla T, Almonacid J, Fernández-Sarría A, Ruiz LA, Recio JA (2010) Combining features extracted from imagery and lidar data for object-oriented classification of forest areas. Int Arch Photogramm Remote Sens Spat Inf Sci 38(4/C7)Hernández Orallo J, Ramírez Quintana MJ, Ferri Ramírez C (2004) Introducción a la minería de datos. Pearson Educación S.A, MadridHomer C, Huang C, Yang L, Wylie B, Coan M (2004) Development of a 2001 National Land-Cover Database for the United States. Photogramm Eng Remote Sens 70:829–840Huberty CJ (1994) Applied discriminant analysis. Wiley, New YorkLaws KI (1985) Goal-directed texture image segmentation. Appl Artif Intel II, SPIE 548:19–26Ormeci C, Alganci U, Sertel E (2010) Identification of crop areas using SPOT-5 data, FIG Congress 2010 Facing the Challenges—building the capacity. Sydney, Australia, pp 11–16Peled A, Gilichinsky M (2004) GIS-driven analyses of remotely sensed data for quality assessment of existing land cover classification. Int Arch Photogramm Remote Sens Spat Inf Sci 35Peled A, Gilichinsky M (2010) Knowledge-based classification of land cover for the quality assessment of GIS database. Int Arch Photogramm Remote Sens Spat Inf Sci 38:217–222Perveen F, Nagasawa R, Ali S, Husnain (2008) Evaluation of ASTER spectral bands for agricultural land cover mapping using pixel-based and object-based classification approaches. Int Arch Photogramm Remote Sens Spat Inf Sci 37(4-C1)Petit CC, Lambin EF (2002) Impact of data integration technique on historical land-use/land-cover change: comparing historical maps with remote sensing data in the Belgian Ardennes. Landsc Ecol 17:117–132Quinlan JR (1993) C4.5: Programs for machine learning. Kaufmann, San FranciscoRabe A, van der Linden S, Hostert P (2010) imageSVM, Version 2.1. www.hu-geomatics.deRecio JA, Hermosilla T, Ruiz LA, Fernández-Sarría A (2011) Historical land use as a feature for image classification. Photogramm Eng Remote Sens 77(4):377–387Ruiz LA, Fernández-Sarría A, Recio JA (2004) Texture feature extraction for classification of remote sensing data using wavelet decomposition: a comparative study. Int Arch Photogramm Remote Sens Spat Inf Sci 35(B4):1109–1115Ruiz LA, Recio JA, Hermosilla T, Fdez. Sarriá A (2009) Identification of agricultural and land cover database changes using object-oriented classification techniques. 33rd International Symposium on Remote Sensing of Environment, May 4–8, Stresa (Italy)Ruiz LA, Recio JA, Fernández-Sarría A, Hermosilla T (2011) A feature extraction software tool for agricultural object-based image analysis. Comput Electron Agric 76(4):284–296Tansey K, Chambers I, Anstee A, Denniss A, Lamb A (2009) Object-oriented classification of very high resolution airborne imagery for the extraction of hedgerows and field margin cover in agricultural areas. Appl Geogr 29(2):145–157van der Linden S, Rabe A, Wirth F, Suess S, Okujeni A, Hostert P (2010) imageSVM regression, application manual: imageSVM version 2.1. Humboldt-Universität zu Berlin, GermanyVapnik VN (1998) Statistical learning theory. Wiley, New YorkWalsh SJ, McCleary AL, Mena CF, Shao Y, Tuttle JP, Gonzalez A, Atkinson R (2008) QuickBird and Hyperion data analysis of an invasive plant species in the Galapagos Islands of Ecuador: implications for control and land use management. Remote Sens Environ 112(5):1927–1941Walter V (2004) Object-based classification of remote sensing data for change detection. ISPRS J Photogramm Remote Sens 58:225–238Walter V (2005) Object-based evaluation of lidar and multiespectral data for automatic change detection in GIS databases. Geo-Inf Syst 18:10–15Zaragozí, B, Rabasa, A, Rodríguez-Sala, JJ, Navarro, JT, Belda, A, Ramón, A (2012) Modelling farmland abandonment: A study combining GIS and data mining techniques. Agric Ecosys Environ 155:124–132Zhang S, Liu X (2005) Realization of data mining model for expert classification using multi-scale spatial data. Int Arch Photogramm Remote Sens Spat Inf Sci 26(4/W6):107–11

Education and inequality in Finland, Spain and Brazil

Production of INCASI Project H2020-MSCA-RISE-2015 GA 691004Finland, Spain and Brazil are three very internally complex and heterogeneous realities, with contradictions and permanent reforms to their education systems. In a first quantitative approach each country can be placed in a continuum of the education system that goes from most successful in terms of reaching a high level of education all across the population, in conditions of equity and facilitating youths' incorporation into the labour market, to least successful, with Finland and Brazil occupying either end of the spectrum respectively and Spain occupying an intermediate situation. Although there are differences, they share certain tensions in their respective education systems. On the one hand, about the conception of education, ranging from more utilitarian, human capital theories, to the more humanist and civic-minded perspective. On the other hand, the challenge of comprehensiveness between an academic and a vocational path. In addition, there is also the challenge of improving the education level of the population while also improving equality. The tensions differ from country to country, since their education traditions and cooperation and conflict strategies between the education agents, with varying levels of resources and different alliances with political actors vary, as does the social consensus

Non-motor symptom burden in patients with Parkinson's disease with impulse control disorders and compulsive behaviours : results from the COPPADIS cohort

The study was aimed at analysing the frequency of impulse control disorders (ICDs) and compulsive behaviours (CBs) in patients with Parkinson's disease (PD) and in control subjects (CS) as well as the relationship between ICDs/CBs and motor, nonmotor features and dopaminergic treatment in PD patients. Data came from COPPADIS-2015, an observational, descriptive, nationwide (Spain) study. We used the validated Questionnaire for Impulsive-Compulsive Disorders in Parkinson's Disease-Rating Scale (QUIP-RS) for ICD/CB screening. The association between demographic data and ICDs/CBs was analyzed in both groups. In PD, this relationship was evaluated using clinical features and treatment-related data. As result, 613 PD patients (mean age 62.47 ± 9.09 years, 59.87% men) and 179 CS (mean age 60.84 ± 8.33 years, 47.48% men) were included. ICDs and CBs were more frequent in PD (ICDs 12.7% vs. 1.6%, p < 0.001; CBs 7.18% vs. 1.67%, p = 0.01). PD patients had more frequent previous ICDs history, premorbid impulsive personality and antidepressant treatment (p < 0.05) compared with CS. In PD, patients with ICDs/CBs presented younger age at disease onset, more frequent history of previous ICDs and premorbid personality (p < 0.05), as well as higher comorbidity with nonmotor symptoms, including depression and poor quality of life. Treatment with dopamine agonists increased the risk of ICDs/CBs, being dose dependent (p < 0.05). As conclusions, ICDs and CBs were more frequent in patients with PD than in CS. More nonmotor symptoms were present in patients with PD who had ICDs/CBs compared with those without. Dopamine agonists have a prominent effect on ICDs/CBs, which could be influenced by dose

Spread of a SARS-CoV-2 variant through Europe in the summer of 2020.

Following its emergence in late 2019, the spread of SARS-CoV-21,2 has been tracked by phylogenetic analysis of viral genome sequences in unprecedented detail3–5. Although the virus spread globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, travel within Europe resumed in the summer of 2020. Here we report on a SARS-CoV-2 variant, 20E (EU1), that was identified in Spain in early summer 2020 and subsequently spread across Europe. We find no evidence that this variant has increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant’s success. Despite travel restrictions, we estimate that 20E (EU1) was introduced hundreds of times to European countries by summertime travellers, which is likely to have undermined local efforts to minimize infection with SARS-CoV-2. Our results illustrate how a variant can rapidly become dominant even in the absence of a substantial transmission advantage in favourable epidemiological settings. Genomic surveillance is critical for understanding how travel can affect transmission of SARS-CoV-2, and thus for informing future containment strategies as travel resumes. © 2021, The Author(s), under exclusive licence to Springer Nature Limited