150 research outputs found
Neuron arbor geometry is sensitive to the limited-range fractal properties of their dendrites
12 pagesFractal geometry is a well-known model for capturing the multi-scaled complexity of
many natural objects. By analyzing three-dimensional images of pyramidal neurons
in the rat hippocampus CA1 region, we examine how the individual dendrites within
the neuron arbor relate to the fractal properties of the arbor as a whole. We find that
the dendrites reveal unexpectedly mild fractal characteristics quantified by a low
fractal dimension. This is confirmed by comparing two fractal methods—a traditional
“coastline” method and a novel method that examines the dendrites’ tortuosity
across multiple scales. This comparison also allows the dendrites’ fractal geometry to
be related to more traditional measures of their complexity. In contrast, the arbor’s
fractal characteristics are quantified by a much higher fractal dimension. Employing
distorted neuron models that modify the dendritic patterns, deviations from natural
dendrite behavior are found to induce large systematic changes in the arbor’s
structure and its connectivity within a neural network. We discuss how this
sensitivity to dendrite fractality impacts neuron functionality in terms of balancing
neuron connectivity with its operating costs. We also consider implications for
applications focusing on deviations from natural behavior, including pathological
conditions and investigations of neuron interactions with artificial surfaces in human
implants
Investigating Fractal Analysis as a Diagnostic Tool That Probes the Connectivity of Hippocampal Neurons
12 pagesMany of nature’s fractal objects benefit from the favorable functionality that results from
their pattern repetition at multiple scales. Our recent research focused on the importance
of fractal scaling in establishing connectivity between neurons. Fractal dimension DA of the
neuron arbors was shown to relate to the optimization of competing functional
constraints—the ability of dendrites to connect to other neurons versus the costs
associated with building the dendrites. Here, we consider whether pathological states
of neurons might affect this fractal optimization and if changes in DA might therefore be
used as a diagnostic tool in parallel with traditional measures like Sholl analyses. We use
confocal microscopy to obtain images of CA1 pyramidal neurons in the coronal plane of
the dorsal rat hippocampus and construct 3-dimensional models of the dendritic arbors
using Neurolucida software. We examine six rodent groups which vary in brain condition
(whether they had lesions in the anterior thalamic nuclei, ATN) and experience (their
housing environment and experience in a spatial task). Previously, we showed ATN lesions
reduced spine density in hippocampal CA1 neurons, whereas enriched housing increased
spine density in both ATN lesion and sham rats. Here, we investigate whether ATN lesions
and experience also effect the complexity and connectivity of CA1 dendritic arbors. We
show that sham rats exposed to enriched housing and spatial memory training exhibited
higher complexity (as measured by DA) and connectivity compared to other groups. When
we categorize the rodent groups into those with or without lesions, we find that both
categories achieve an optimal balance of connectivity with respect to material cost.
However, the DA value used to achieve this optimization does not change between
these two categories, suggesting any morphological differences induced by the lesions are
too small to influence the optimization process. Accordingly, we highlight considerations
associated with applying our technique to publicly accessible repositories of neuron
images with a broader range of pathological conditions
Sex differences in the Simon task help to interpret sex differences in selective attention.
In the last decade, a number of studies have reported sex differences in selective attention, but a unified explanation for these effects is still missing. This study aims to better understand these differences and put them in an evolutionary psychological context. 418 adult participants performed a computer-based Simon task, in which they responded to the direction of a left or right pointing arrow appearing left or right from a fixation point. Women were more strongly influenced by task-irrelevant spatial information than men (i.e., the Simon effect was larger in women, Cohen's d = 0.39). Further, the analysis of sex differences in behavioral adjustment to errors revealed that women slow down more than men following mistakes (d = 0.53). Based on the combined results of previous studies and the current data, it is proposed that sex differences in selective attention are caused by underlying sex differences in core abilities, such as spatial or verbal cognition
Moving beyond neurons: the role of cell type-specific gene regulation in Parkinson's disease heritability
Parkinson’s disease (PD), with its characteristic loss of nigrostriatal dopaminergic neurons and deposition of α-synuclein in neurons, is often considered a neuronal disorder. However, in recent years substantial evidence has emerged to implicate glial cell types, such as astrocytes and microglia. In this study, we used stratified LD score regression and expression-weighted cell-type enrichment together with several brain-related and cell-type-specific genomic annotations to connect human genomic PD findings to specific brain cell types. We found that PD heritability attributable to common variation does not enrich in global and regional brain annotations or brain-related cell-type-specific annotations. Likewise, we found no enrichment of PD susceptibility genes in brain-related cell types. In contrast, we demonstrated a significant enrichment of PD heritability in a curated lysosomal gene set highly expressed in astrocytic, microglial, and oligodendrocyte subtypes, and in LoF-intolerant genes, which were found highly expressed in almost all tested cellular subtypes. Our results suggest that PD risk loci do not lie in specific cell types or individual brain regions, but rather in global cellular processes detectable across several cell types
The genetic architecture of the human cerebral cortex
The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson’s disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder
Use of anticoagulants and antiplatelet agents in stable outpatients with coronary artery disease and atrial fibrillation. International CLARIFY registry
The genetic architecture of the human cerebral cortex
INTRODUCTION
The cerebral cortex underlies our complex cognitive capabilities. Variations in human cortical surface area and thickness are associated with neurological, psychological, and behavioral traits and can be measured in vivo by magnetic resonance imaging (MRI). Studies in model organisms have identified genes that influence cortical structure, but little is known about common genetic variants that affect human cortical structure.
RATIONALE
To identify genetic variants associated with human cortical structure at both global and regional levels, we conducted a genome-wide association meta-analysis of brain MRI data from 51,665 individuals across 60 cohorts. We analyzed the surface area and average thickness of the whole cortex and 34 cortical regions with known functional specializations.
RESULTS
We identified 306 nominally genome-wide significant loci (P < 5 × 10−8) associated with cortical structure in a discovery sample of 33,992 participants of European ancestry. Of the 299 loci for which replication data were available, 241 loci influencing surface area and 14 influencing thickness remained significant after replication, with 199 loci passing multiple testing correction (P < 8.3 × 10−10; 187 influencing surface area and 12 influencing thickness).
Common genetic variants explained 34% (SE = 3%) of the variation in total surface area and 26% (SE = 2%) in average thickness; surface area and thickness showed a negative genetic correlation (rG = −0.32, SE = 0.05, P = 6.5 × 10−12), which suggests that genetic influences have opposing effects on surface area and thickness. Bioinformatic analyses showed that total surface area is influenced by genetic variants that alter gene regulatory activity in neural progenitor cells during fetal development. By contrast, average thickness is influenced by active regulatory elements in adult brain samples, which may reflect processes that occur after mid-fetal development, such as myelination, branching, or pruning. When considered together, these results support the radial unit hypothesis that different developmental mechanisms promote surface area expansion and increases in thickness.
To identify specific genetic influences on individual cortical regions, we controlled for global measures (total surface area or average thickness) in the regional analyses. After multiple testing correction, we identified 175 loci that influence regional surface area and 10 that influence regional thickness. Loci that affect regional surface area cluster near genes involved in the Wnt signaling pathway, which is known to influence areal identity.
We observed significant positive genetic correlations and evidence of bidirectional causation of total surface area with both general cognitive functioning and educational attainment. We found additional positive genetic correlations between total surface area and Parkinson’s disease but did not find evidence of causation. Negative genetic correlations were evident between total surface area and insomnia, attention deficit hyperactivity disorder, depressive symptoms, major depressive disorder, and neuroticism.
CONCLUSION
This large-scale collaborative work enhances our understanding of the genetic architecture of the human cerebral cortex and its regional patterning. The highly polygenic architecture of the cortex suggests that distinct genes are involved in the development of specific cortical areas. Moreover, we find evidence that brain structure is a key phenotype along the causal pathway that leads from genetic variation to differences in general cognitive function
The genetic architecture of the human cerebral cortex
The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder
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