Assessment of labelled products with different radioanalytical methods: study on 18F-fluorination reaction of 4-[18F]fluoro-N-[2-[1-(2-methoxyphenyl)-1-piperazinyl]ethyl-N-2-pyridinyl-benzamide (p-[18F]MPPF)

Peer reviewe

Association between quality of life and severity of profile deviation in prospective orthognathic patients

Aimto evaluate whether severity of skeletal facial profile deviation assessed by professionals associates with quality of life and psychosocial factors in patients with dentofacial deformities and prospective orthognathic treatment.Materials and methodThe study consisted of 55 patients admitted to orthognathic treatment. Skeletal profile was assessed from lateral head films using cephalometric analysis. The following angles were used to assess the sagittal position of upper and lower jaw and profile: Sella-Nasion-A-point-angle, Sella-Nasion-B-point-angle, and A-point-Nasion-B-point-angle (ANB). For vertical assessment, gonial angle and the angle between Sella-Nasion and mandibular plane were used. Merrifield’s Z-angle was used to assess soft-tissue profile. Severity of skeletal facial profile deviation was assessed with deviation in ANB angle. Orthognathic quality of life (OQoL) and psychosocial factors were defined with four questionnaires: Orthognathic Quality of Life Questionnaire, Symptom Checklist-90, Rosenberg Self-Esteem Scale, and a body image questionnaire.ResultsIncrease in ANB deviation was associated with increased awareness of dentofacial deformity [Orthognathic Quality of Life Questionnaire subscale awareness of dentofacial deformity (OQLQ-AoDD), r = 0.319, P = 0.017). OQOL-AoDD was not found to be equal when ANB angle was divided into three different categories (χ 2 = 6.78, P = 0.034): G1. ANB = 0–4 degrees; G2. ANB 4 degrees. Furthermore, categories G1 and G2 differed significantly (U = 50.5, P = 0.017). Increase in ANB angle was also associated with a more positive body image (r = 0.342, P = .023). There were no significant correlations between other cephalometric variables, quality of life, and psychosocial factors.ConclusionSkeletal facial profile seems to associate with some aspects of orthognathic quality of life. Professional cephalometric analysis of the severity of facial profile deviation correlates with patients’ awareness of their own facial and dental appearance. Patients with more deviating skeletal profile are more aware of their dentofacial deformities compared to patients with normal values.</div

Robotics in Care Services : A Finnish Roadmap

Julkaisu löytyy myös osoitteesta; http://roseproject.aalto.fi/images/publications/Roadmap-final02062017.pd

Pathological consequences of VCP mutations on human striated muscle

*These authors have contributed equally to this work. Mutations in the valosin-containing protein (VCP, p97) gene on chromosome 9p13-p12 cause a late-onset form of autosomal dominant inclusion body myopathy associated with Paget disease of the bone and frontotemporal dementia (IBMPFD). We report on the pathological consequences of three heterozygous VCP (R93C, R155H, R155C) mutations on human striated muscle. IBMPFD skeletal muscle pathology is characterized by degenerative changes and filamentous VCP-and ubiquitin-positive cytoplasmic and nuclear protein aggregates. Furthermore, this is the first report demonstrating that mutant VCP leads to a novel form of dilatative cardiomyopathy with inclusion bodies. In contrast to post-mitotic striated muscle cells and neurons of IBMPFD patients, evidence of protein aggregate pathology was not detected in primary IBMPFD myoblasts or in transient and stable transfected cells using wild-type-VCP and R93C-, R155H-, R155C-VCP mutants. Glutathione S-transferase pull-down experiments showed that all three VCP mutations do not affect the binding to Ufd1, Npl4 and ataxin-3. Structural analysis demonstrated that R93 and R155 are both surface-accessible residues located in the centre of cavities that may enable ligand-binding. Mutations at R93 and R155 are predicted to induce changes in the tertiary structure of the VCP protein. The search for putative ligands to the R93 and R155 cavities resulted in the identification of cyclic sugar compounds with high binding scores. The latter findings provide a novel link to VCP carbohydrate interactions in the complex pathology of IBMPFD. Keywords: VCP; p97; myopathy; cardiomyopathy; IBMPFD Abbreviations: GST = glutathione S-transferase; IBMPFD = inclusion body myopathy associated with Paget disease of the bone and frontotemporal dementia; PBS = phosphate-buffered saline; SDS = sodium dodecyl sulphate; VCP = valosin-containing protei

Seurantakäsikirja Suomen merenhoitosuunnitelman seurantaohjelmaan vuosille 2020–2026

Tämä merenhoidon seurantakäsikirja käsittää merenhoitosuunnitelman seurantaohjelman kuvauksen kokonaisuudessaan. Se päivittää vuoden 2014–2020 seurantaohjelman ja sitä sovelletaan vuoden 2020 heinäkuusta vuoden 2026 heinäkuuhun. Seurantaohjelma on osa merenhoidon suunnittelua, jota tehdään vesienhoidon ja merenhoidon järjestämisestä annetun lain (272/2011) ja merenhoidon järjestämisestä annetun valtioneuvoston asetuksen (980/2011) toteuttamiseksi. Tämä laki ja asetus on annettu meristrategiadirektiivin (Euroopan parlamentin ja neuvoston direktiivi 2008/56/EY yhteisön meriympäristöpolitiikan puitteista) kansallista toimeenpanoa varten. Suomessa meristrategiadirektiivin mukaista meristrategiaa kutsutaan merenhoitosuunnitelmaksi. Suomen seurantaohjelma koostuu 13:sta ohjelmasta, joiden alla on yhteensä 44 alaohjelmaa. Tähän päivitettyyn seurantaohjelmaan lisättiin kuusi uutta alaohjelmaa ja useita alaohjelmia muokattiin joko muuttuneiden vaatimusten, kehittyneempien menetelmien tai muuttuneen toimintaympäristön takia. Merenhoidon uusia vaatimuksia ovat meristrategiadirektiivin liitteen 3 päivitys (EU/2017/845), Euroopan komission päätös EU/2017/848 merivesien hyvän ekologisen tilan vertailuperusteista ja menetelmästandardeista sekä seurantaa ja arviointia varten tarkoitetut täsmennykset standardoiduista menetelmistä. Seurantakäsikirja koostuu kolmesta osasta: seurantaohjelman tausta, varsinainen seurantaohjelma, ja kolmas osa, joka käsittelee seurannan kehitystarpeita, kustannuksia ja riittävyyttä. Seurantaohjelma kattaa ekosysteemilähestymistavan mukaisesti erilaisia muuttujia, jotka kuvaavat toisaalta veden ominaisuuksia ja laatua ja toisaalta ekosysteemin osia ja niiden tilaa sekä niihin kohdistuvia ihmisestä johtuvia paineita. Seurannan alaohjelmissa on kuvattu mitattavat meriympäristön ominaisuudet tai paineet, niiden seurantatiheys, indikaattorit, joihin seurantatietoa käytetään, seurannalla kootun tiedon hallinta ja yhteydet meristrategiadirektiivin hyvän tilan laadullisiin kuvaajiin ja kriteereihin

Terveyden edistäminen Oulun yliopistollisessa sairaalassa

Tutkimuksen tarkoituksena oli kuvata Oulun yliopistollisen sairaalan neljän tulosalueen terveydenhuollon ammattihenkilöiden arvioita toiminnastaan potilaiden terveyden edistämiseksi. Lisäksi tarkoituksena oli selvittää heidän mielipiteitään ja kokemuksiaan terveyden edistämisestä, siinä käytetyistä menetelmistä ja terveyden edistämiseen liittyvistä kehittämistarpeista. Tutkimus tehtiin Pirkanmaan sairaanhoitopiirissä tehdyn vastaavan kyselytutkimuksen pohjalta. Tutkimuksen tavoitteena oli tuottaa uutta tietoa potilaiden terveyden edistämisen nykytilasta, jota Oysin perusterveydenhuollon yksikön terveyden edistämisen työryhmä voi käyttää terveyden edistämisen suunnittelutyön tukena. Kysymykset liittyivät alkoholin riskikäyttöön, tupakointiin, ylipainoon, masennukseen ja perheiden tukemiseen. Tutkimuksen tietoperustassa käsitellään potilaan terveyden edistämiseen liittyvää historiaa, taustaa, käsitteitä sekä lakia. Lisäksi tietoperustassa tarkastellaan terveyden edistämistä yhteiskunnallisesta näkökulmasta. Tutkimus on kvantitatiivinen survey-tutkimus, jossa aineistonhankintamenetelmänä käytettiin webropol-sovellusta. Tutkimuksen vastausprosentti oli 23 % (N=4276), joten tutkimustulokset ovat suuntaa antavia. Tutkimustulosten perusteella voidaan todeta, että terveydenedistäminen on jokaisen sairaalassa työskentelevän tehtävä ja sitä tehdäkseen henkilökunnalle olisi tarjottava enemmän resursseja ja täydennyskoulutusta. Tutkimuksen kehittämisideana oli tuoda esille nykyisten käytäntöjen vahvuudet ja kehittämisalueet, joita edelleen kehittämällä voidaan tuottaa laadukkaampia ja tehokkaampia terveyden edistämisen käytäntöjä erikoissairaanhoidossa. Jatkotutkimuksena voitaisiin tutkia erikoissairaanhoidossa olevien potilaiden ja heidän omaistensa kokemuksia, mielipiteitä ja toiveita terveyden edistämiseksi esimerkiksi palvelumuotoilun avulla.The purpose of this study was to describe the evaluations of health care professionals from four different departments at the Oulu University hospital on their actions in promoting the health of patients. In addition, the aim was to investigate their opinions and experiences in health promoting, the methods used for it and the needs for future development. The study was carried out in the same manner as a report done at the Pirkanmaa health care district. The purpose of the study was to obtain information on the present status of health promotion at the Oulu University hospital. The report is also intended to support the work of the health promotion work group at the unit of basic health care. The questions of the study were related to alcohol consumption, smoking, obesity, depression and family support. The knowledge basis part of the work consists the history, background, concepts and law of health promotion. The knowledge basis contains also evaluation of health promotion from the societal perspective. The study is a quantitative survey where webropol application was used for material acquisition. The response rate of the survey was 23 % (N=4276), meaning that the results are directional. The results show that the personnel working at hospitals need to promote health and in order to succeed; the personnel require more resources and supplemental education. The development idea of the study was to highlight the strengths and weaknesses of the present health promotion practices. Improvements in these areas can then provide higher quality and more efficient methods for promoting health in special health care. The present study could be followed by a survey of the experiences, opinions and expectations on health promotion of patients and their relatives undergoing special health care.Muita tekijöitä on lisäksi: Teija Mykkänen ja Marjukka Salonen-Jänkäl

Allosteric mechanism for site-specific ubiquitination of FANCD2

DNA-damage repair is implemented by proteins that are coordinated by specialized molecular signals. One such signal in the Fanconi anemia (FA) pathway for the repair of DNA interstrand crosslinks is the site-specific monoubiquitination of FANCD2 and FANCI. The signal is mediated by a multiprotein FA core complex (FA-CC) however, the mechanics for precise ubiquitination remain elusive. We show that FANCL, the RING-bearing module in FA-CC, allosterically activates its cognate ubiqutin-conjugating enzyme E2 UBE2T to drive site-specific FANCD2 ubiquitination. Unlike typical RING E3 ligases, FANCL catalyzes ubiquitination by rewiring the intraresidue network of UBE2T to influence the active site. Consequently, a basic triad unique to UBE2T engages a structured acidic patch near the target lysine on FANCD2. This three-dimensional complementarity, between the E2 active site and substrate surface, induced by FANCL is central to site-specific monoubiquitination in the FA pathway. Furthermore, the allosteric network of UBE2T can be engineered to enhance FANCL-catalyzed FANCD2–FANCI di-monoubiquitination without compromising site specificity

Customer value creation in the development of digital health services:discourse analysis

Abstract The study’s aim was to describe how customer value creation is reflected in the development of digital health services. To this end, we used discourse analysis to evaluate documentation from the ODA (Self-Treatment and Digital Value Services) project, which provides national-scale digital health services. Three main discourses emerged: 1) a discourse on the active role of the customer, 2) a discourse on technology that activates the customer to create value, and 3) a discourse on the benefits of customer value creation. The research provided new insight into customer value creation in digital health service development. Speech about customer value creation was a part of the social reality of the digital health service’s development. The customer appeared as an active player and a key resource within the service. The role of the active customer was considered demanding and responsible. Our findings suggest that the new digital service changed customer behavior, with technology acting as an enabler of this change. Customer activity and information sharing were seen as enablers of value creation and the associated benefits, and there was a willingness to strengthen the customer’s role as a need determiner

Pathological consequences of VCP mutations on human striated muscle.

Mutations in the valosin-containing protein (VCP, p97) gene on chromosome 9p13-p12 cause a late-onset form of autosomal dominant inclusion body myopathy associated with Paget disease of the bone and frontotemporal dementia (IBMPFD). We report on the pathological consequences of three heterozygous VCP (R93C, R155H, R155C) mutations on human striated muscle. IBMPFD skeletal muscle pathology is characterized by degenerative changes and filamentous VCP- and ubiquitin-positive cytoplasmic and nuclear protein aggregates. Furthermore, this is the first report demonstrating that mutant VCP leads to a novel form of dilatative cardiomyopathy with inclusion bodies. In contrast to post-mitotic striated muscle cells and neurons of IBMPFD patients, evidence of protein aggregate pathology was not detected in primary IBMPFD myoblasts or in transient and stable transfected cells using wild-type-VCP and R93C-, R155H-, R155C-VCP mutants. Glutathione S-transferase pull-down experiments showed that all three VCP mutations do not affect the binding to Ufd1, Npl4 and ataxin-3. Structural analysis demonstrated that R93 and R155 are both surface-accessible residues located in the centre of cavities that may enable ligand-binding. Mutations at R93 and R155 are predicted to induce changes in the tertiary structure of the VCP protein. The search for putative ligands to the R93 and R155 cavities resulted in the identification of cyclic sugar compounds with high binding scores. The latter findings provide a novel link to VCP carbohydrate interactions in the complex pathology of IBMPFD