2-(4-Meth­oxy­phen­yl)-4H-1,3,2-benzoxathia­phosphinine 2-sulfide

The asymmetric unit of the title compound, C14H13O2PS2, contains two crystallographically independent mol­ecules, which differ in the conformation of the 1,3,2-benzoxathia­phosphinine moieties (screw boat in the first mol­ecule and envelope in the second mol­ecule). In the crystal, neither classical nor non-classical hydrogen bonds are found. Weak inter­actions (about 2.9–3.0 Å) between the lone pair of the terminal S atoms with H atoms occur. This compound was further characterized by 1H NMR and IR spectroscopy

2-[2-(1H-Imidazol-1-yl)-2-adamant­yl]phenol

In the title mol­ecule, C19H22N2O, the imidazole and benzene rings form a dihedral angle of 84.53 (5)°. In the crystal, classical inter­molecular O—H⋯N hydrogen bonds pair the mol­ecules into centrosymmetric dimers, and C—H⋯π inter­actions further link these dimers into columns propagated in [100]

Marker Loci in Brucella Genome for Differential PCR Indication of Pathogenic strains

Objective of this work was to develop the algorithms for differential PCR indication of Brucella genus strains using databases of their genomes. Materials and methods .Resources of the National Center for Biotechnology Information (NCBI) and BLAST and Vector NTI 9.1.0 software utilities. For PCR amplification, B. suis, B. abortus, B. melitensis nucleic acids, as well as plasmid DNA with marker insertions were used. Results and conclusions. We assessed brucella gene sequences, some of which are found in Brucella genus bacteria, others only in representatives of B. melitensis, and the third ones – only in representatives of B. abortus. As a result of primers and probes designing for indication of Brucella genus bacteria and representatives of B. melitensis and B. abortus species, criteria for marker sequence amplification have been established. These criteria provide for simultaneous differentiation in a single reaction. The determination of strain differences within one species of Brucella is described in multilocus VNTR assay technique, and the profiles of tandem repeats of various B. melitensis and B. abortus strains are available in the public domain. To monitor the progress of amplification, a positive control has been developed that has the nucleotide sequence of all marker regions. The text of the paper discloses all the nucleotide sequences of primers, probes and positive control, which makes it possible to independently acquire them in competent organizations

Сполиготипирование туберкулезных микобактерий, выделенных от человека и крупного рогатого скота

Objective: to analyze the species and genetic families of tuberculous mycobacteria isolated from humans and cattle by spoligotyping.Subjects and methods. Biological materials collected in the cattle, livestock farmers (who underwent annual fluorographic examinations) and tuberculosis patients were studied. To identify mycobacteria, the spoligotyping method was used.Results. The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem.Цель исследования: анализ видов и генетических линий туберкулезных микобактерий, выделенных от человека и крупного рогатого скота, 2 методом сполиготипирования.Материалы и методы. Объектом исследования служил биологический материал от крупного рогатого скота, а также работников животноводческих ферм (ежегодно проходивших флюорографическое исследование) и больных туберкулезом пациентов. Для идентификации микобактерий использовали метод сполиготипирования.Результаты. Представлена оценка вариабельности участков внутри прямых повторов (спейсеров), которые в лабораторной диагностике используются при сполиготипировании микобактерий и идентификации возбудителей туберкулеза. Сопоставление встречаемости комбинаций различных спейсеров у анализируемых микобактерий в исследуемом материале, идентифицированных как Mycobacteriumtuberculosiscomplex, со сполигопрофилем известных микобактерий устанавливает принадлежность их к конкретной генетической линии. Исследованные изоляты изучены микроскопическими, бактериологическими и молекулярно-генетическими (ПЦР) методами. По результатам сполиготипирования определена их принадлежность к генетическим линиям M. tuberculosisBeijing, LAM и Haarlem

Six-membered ring systems: with O and/or S atoms

A large variety of publications have emerged in 2012 involving O- and S-6- membered ring systems. The increasing number of reviews and other communica- tions dedicated to natural and synthetic derivatives and their biological significance highlights the importance of these heterocycles. Reviews on natural products involve biosynthesis and isolation of enantiomeric derivatives h12AGE4802i, biosynthesis, isolation, synthesis, and biological studies on the pederin family h12NPR980i and xanthones obtained from fungi, lichens, and bacteria h12CR3717i and on the potential chemotherapeutic value of phyto- chemical products and plant extracts as antidiabetic h12NPR580i, antimicrobial, and resistance-modifying agents h12NPR1007i. A more specific review covers a structure–activity relationship of endoperoxides from marine origin and their antitry- panosomal activity h12OBC7197i. New synthetic routes to naturally occurring, biologically active pyran derivatives have been the object of several papers. Different approaches have been discussed for the total synthesis of tetrahydropyran-containing natural products (")-zampanolide h12CEJ16868, 12EJO4130, 12OL3408i, (")-aspergillides A and B h12H(85)587, 12H(85)1255, 12TA252i, (þ)-neopeltolide h12JOC2225, 12JOC9840, 12H(85) 1255i, or their macrolactone core h12OBC3689, 12OL2346i. The total synthesis of bistramide A h12CEJ7452i and (þ)-kalihinol A h12CC901i and the stereoselec- tive synthesis of a fragment of bryostatin h12S3077, 12TL6163i have also been sur- veyed. Other papers relate the total synthesis of naturally occurring carbocyclic and heterocyclic-fused pyran compounds, such as (")-dysiherbaine h12CC6295i, penos- tatin B h12OL244i, Greek tobacco lactonic products, and analogues h12TL4293i and on the structurally intriguing limonoids andhraxylocarpins A–E h12CEJ14342i. The stereocontrolled synthesis of fused tetrahydropyrans was used in the preparation of blepharocalyxin D h12AGE3901i. Polyphenolic heterocyclic compounds have also received great attention in 2012. The biological activities and the chemistry of prenylated caged xanthones h12PCB78i, the occurrence of sesquiterpene coumarins h12PR77i, and the medicinal properties of the xanthone mangiferin h12MRME412i have been reviewed. An overview on the asymmetric syntheses of flavanones and chromanones h12EJO449i, on the synthesis and reactivity of flavones h12T8523i and xanthones h12COC2818i, on the synthesis and biosynthesis of biocoumarins h12T2553i, and on the synthesis and applications of flavylium compounds h12CSR869i has been discussed. The most recent developments in the synthesis and applications of sultones, a very important class of sulfur compounds, were reported h12CR5339i. A review on xanthene-based fluorescent probes for sensing cations, anions, bio- logical species, and enzyme activity has described the spiro-ring-opening approach with a focus on the major mechanisms controlling their luminescence behavior h12CR1910i. The design and synthesis of other derivatives to be used as sensors of gold species h12CC11229i and other specific metal cations h12PC823i have also been described. Recent advances related to coumarin-derived fluorescent chemosen- sors for metal ions h12COC2690i and to monitoring in vitro analysis and cellular imaging of monoamine oxidase activity h12CC6833i have been discussed. The study of various organic chromophores allowed the synthesis of novel dica- tionic phloroglucinol-type bisflavylium pigments h12SL2053i, and the optical and spectroscopic properties of several synthetic 6-aryldibenzo[b,d]pyrylium salts were explored h12TL6433i. Discussion of specific reactions leading to O- and S-membered heterocyclic compounds covers intramolecular radical cyclization h12S2475i and asymmetric enamine and dienamine catalysis h12EJO865i, oxa-Michael h12CSR988i and dom- ino Knoevenagel–hetero-Diels–Alder (hDA) reactions h12T5693i, and the versatility in cycloadditions as well as nucleophilic reactions using o-quinones h12CSR1050i. The use of specific reagents relevant to this chapter includes molecular iodine h12CEJ5460, 12COS561i, samarium diiodide–water for selective reductive transfor- mations h12CC330i, o-quinone methides as versatile intermediates h12CEJ9160i, InCl3 as catalyst h12T8683i, and gold and platinum p-acid mediated insertion of alkynes into carbon–heteroatom s-bonds h12S3401i. The remainder of this chapter discusses the most studied transformations on O- and S-6-membered heterocycles

Modern Trends of Organic Chemistry in Russian Universities

© 2018, Pleiades Publishing, Ltd. This review is devoted to the scientific achievements of the departments of organic chemistry in higher schools of Russia within the past decade