Combined grazing incidence RBS and TEM analysis of luminescent nano-SiGe/SiO2 multilayers.

Multilayer structures with five periods of amorphous SiGe nanoparticles/SiO2 layers with different thickness were deposited by Low Pressure Chemical Vapor Deposition and annealed to crystallize the SiGe nanoparticles. The use of grazing incidence RBS was necessary to obtain sufficient depth resolution to separate the signals arising from the individual layers only a few nm thick. The average size and areal density of the embedded SiGe nanoparticles as well as the oxide interlayer thickness were determined from the RBS spectra. Details of eventual composition changes and diffusion processes caused by the annealing processes were also studied. Transmission Electron Microscopy was used to obtain complementary information on the structural parameters of the samples in order to check the information yielded by RBS. The study revealed that annealing at 900 °C for 60 s, enough to crystallize the SiGe nanoparticles, leaves the structure unaltered if the interlayer thickness is around 15 nm or higher

Influence of the crystallization process on the luminescence of multilayers of SiGe nanocrystals embedded in SiO2

Multilayers of SiGe nanocrystals embedded in an oxide matrix have been fabricated by low-pressure chemical vapor deposition SiO2 onto Si wafers (in a single run at 390 ◦C and 50mTorr, using GeH4, Si2 H6 and O2) followed by a rapid thermal annealing crystallize the SiGe nanoparticles. The main emission band is located at 400 nm in both cathodoluminescence and photoluminescence at 80K and also at room temperature. The annealing conditions (temperatures ranging from 700 to 1000 ◦C and for times of 30 investigated in samples with different diameter of the nanoparticles (from ≈3 to ≥5 nm) and oxide interlayer thickness (15 and establish a correlation between the crystallization of the nanoparticles, the degradation of their composition by Ge diffusion the luminescence emission band. Structures with small nanoparticles (3–4.5 nm) separated by thick oxide barriers (≈35 nm) annealed 60 s yield the maximum intensity of the luminescence. An additional treatment at 450 ◦C in forming gas for dangling-bond the intensity of the luminescence band by 25–30%

Mapping of landslide susceptibility of coastal cliffs : the Mont-Roig del Camp case study

The weathered and fractured conglomerate cliffs of Mont Roig del Camp constitute a rock fall hazard for the surrounding pocket beaches and, therefore, for the population that frequent them, especially over the summer. Landslide susceptibility of the cliff has been assessed using the Rock Engineering System method (RES). The determinant and triggering factors considered in this study include: wave exposure, shoreline variations, cliff height, cliff slope, geotechnical quality of the rocky mass, superficial runoff and cliff orientations favoring landslides. Geographic Information Systems (GIS) have been employed to facilitate the information analysis and generate new susceptibility maps. The quality of the rock mass and cliff orientation are the most interactive factors for the stability of the cliff. However, shoreline variations and surface runoff are the most dominant factors in the system. Thus, the quality of the rock mass has been determined to be a basic variable in the cliff characterization because of its high dependence on the variations of the remaining factors. The landslide susceptibility map depicts a predominance of surfaces with moderate degrees of susceptibility concentrated mainly in the headlands, where the combined actions of subaerial and marine processes control the weathering and eroding processes. Therefore, the landslide susceptibility assessment based on this methodology has allowed the identification of hazardous areas that should be considered in future management plans

Dinámicas socioecológicas y ecoturismo comunitario: Un análisis comparativo en el eje fluvial Guayabero-Guaviare

The effects of the ecotourism in the relationships between inhabitants and their environment are complex and multidimensional. To explore these effects, two community ecotourism initiatives in the Guaviare Province were comparatively analyzed. This territory has been historically affected by the armed conflict and today is given priority as a scenery for the peace construction. The analysis herein articulates approaches both from the political ecology and the socio-ecological systems and considers how important it is to understand the power relations emerging in community ecotourism contexts that, in turn, help to measure how this kind of tourism impacts the socio-ecological dynamics and the strategies by the peasant families who try to diversify their economies to made them sustainable. © 2018, Pontificia Universidad Javeriana. All rights reserved

Cooperativity of stress-responsive transcription factors in core hypoxia-inducible factor binding regions

The transcriptional response driven by Hypoxia-inducible factor (HIF) is central to the adaptation to oxygen restriction. Despite recent characterization of genome-wide HIF DNA binding locations and hypoxia-regulated transcripts in different cell types, the molecular bases of HIF target selection remain unresolved. Herein, we combined multi-level experimental data and computational predictions to identify sequence motifs that may contribute to HIF target selectivity. We obtained a core set of bona fide HIF binding regions by integrating multiple HIF1 DNA binding and hypoxia expression profiling datasets. This core set exhibits evolutionarily conserved binding regions and is enriched in functional responses to hypoxia. Computational prediction of enriched transcription factor binding sites identified sequence motifs corresponding to several stress-responsive transcription factors, such as activator protein 1 (AP1), cAMP response element-binding (CREB), or CCAAT-enhancer binding protein (CEBP). Experimental validations on HIF-regulated promoters suggest a functional role of the identified motifs in modulating HIF-mediated transcription. Accordingly, transcriptional targets of these factors are over-represented in a sorted list of hypoxia-regulated genes. Altogether, our results implicate cooperativity among stress-responsive transcription factors in fine-tuning the HIF transcriptional responseThis work was supported by Ministerio de Ciencia e Innovación (Spanish Ministry of Science and Innovation, MICINN) [grant number SAF2008-03147 to L. del P.], Comunidad Autónoma de Madrid [grant number S-SAL-0311_2006 to L. del P.] and the 7th Research Framework Programme of the European Union [grant number METOXIA project ref. HEALTH-F2-2009-222741] to L. del P. D.V. was a recipient of PhD funding from the Spanish Ministry of Science and Innovation [FPU programme] and the European Molecular Biology Organization [Short-Term Fellowships

Tomography of Galactic star-forming regions and spiral arms with the Square Kilometre Array

Copyright owned by the author(s) under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike LicenceVery Long Baseline Interferometry (VLBI) at radio wavelengths can provide astrometry accurate to 10 micro-arcseconds or better (i.e. better than the target GAIA accuracy) without being limited by dust obscuration. This means that unlike GAIA, VLBI can be applied to star-forming regions independently of their internal and line-of-sight extinction. Low-mass young stellar objects (particularly T Tauri stars) are often non-thermal compact radio emitters, ideal for astrometric VLBI radio continuum experiments. Existing observations for nearby regions (e.g. Taurus, Ophiuchus, or Orion) demonstrate that VLBI astrometry of such active T Tauri stars enables the reconstruction of both the regions' 3D structure (through parallax measurements) and their internal kinematics (through proper motions, combined with radial velocities). The extraordinary sensitivity of the SKA telescope will enable similar "tomographic mappings" to be extended to regions located several kpc from Earth, in particular to nearby spiral arm segments. This will have important implications for Galactic science, galactic dynamics and spiral structure theories.Final Published versio

Growth Arrest-Specific Factor 6 (GAS6) Is Increased in COVID-19 Patients and Predicts Clinical Outcome

Producción CientíficaBackground: Growth arrest-specific factor 6 (GAS6) and the Tyro3, AXL, and MERTK (TAM) receptors counterbalance pro-inflammatory responses. AXL is a candidate receptor for SARS-CoV-2, particularly in the respiratory system, and the GAS6/AXL axis is targeted in current clinical trials against COVID-19. However, GAS6 and TAMs have not been evaluated in COVID-19 patients at emergency admission. Methods: Plasma GAS6, AXL, and MERTK were analyzed in 132 patients consecutively admitted to the emergency ward during the first peak of COVID-19. Results: GAS6 levels were higher in the SARS-CoV-2-positive patients, increasing progressively with the severity of the disease. Patients with initial GAS6 at the highest quartile had the worst outcome, with a 3-month survival of 65%, compared to a 90% survival for the rest. Soluble AXL exhibited higher plasma concentration in deceased patients, without significant differences in MERTK among SARS-CoV-2-positive groups. GAS6 mRNA was mainly expressed in alveolar cells and AXL in airway macrophages. Remarkably, THP-1 human macrophage differentiation neatly induces AXL, and its inhibition (bemcentinib) reduced cytokine production in human macrophages after LPS challenge. Conclusions: Plasma GAS6 and AXL levels reflect COVID-19 severity and could be early markers of disease prognosis, supporting a relevant role of the GAS6/AXL system in the immune response in COVID-19.Ministerio de Ciencia, Innovación y Universidades (project RTI2018-095672-B-I00)Instituto de Salud Carlos III - Fondo de Investigación Sanitaria (grants PI15/00531 and PI19/01410)Fundació La Marató TV3 (grants 20153030 and 20153031)Consejo Superior de Investigaciones Científicas (project CSIC-COV19-016/202020E155)Junta de Castilla y León (project 07.04.467B04.74011.0

The T-REX project: Micromegas for rare event searches

The T-REX project aims at developing novel readout techniques for Time Projection Chambers for experiments searching for Rare Events. The Micromegas detectors are a good option, because of their good performance regarding low background levels, energy and time resolution, gain and stability of operation. In the present we will shortly refer to two particular cases, on one hand their performance in the CAST experiment and on the other the studies carried out within NEXT, a neutrinoless double-beta decay experiment

The HY5-PIF regulatory module coordinates light and temperature control of photosynthetic gene transcription

The ability to interpret daily and seasonal alterations in light and temperature signals is essential for plant survival. This is particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production for photoautotrophic growth. Phytochromes accomplish this partly through the suppression of phytochrome interacting factors (PIFs), negative regulators of chlorophyll and carotenoid biosynthesis. While the bZIP transcription factor long hypocotyl 5 (HY5), a potent PIF antagonist, promotes photosynthetic pigment accumulation in response to light. Here we demonstrate that by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. This antagonistic regulatory module provides a simple, direct mechanism through which environmental change can redirect transcriptional control of genes required for photosynthesis and photoprotection. In the regulation of photopigment biosynthesis genes, HY5 and PIFs do not operate alone, but with the circadian clock. However, sudden changes in light or temperature conditions can trigger changes in HY5 and PIFs abundance that adjust the expression of common target genes to optimise photosynthetic performance and growth