Sobolev Embeddings for Fractional Hajłasz-Sobolev Spaces in the Setting of Rearrangement Invariant Spaces

Acord transformatiu CRUE-CSICWe obtain symmetrization inequalities in the context of Fractional Hajłasz-Sobolev spaces in the setting of rearrangement invariant spaces and prove that for a large class of measures our symmetrization inequalities are equivalent to the lower bound of the measure

A Sobolev type embedding theorem for Besov spaces defined on doubling metric spaces

We obtain a Sobolev type embedding result for Besov spaces defined on a doubling measure metric space

Mapping of landslide susceptibility of coastal cliffs : the Mont-Roig del Camp case study

The weathered and fractured conglomerate cliffs of Mont Roig del Camp constitute a rock fall hazard for the surrounding pocket beaches and, therefore, for the population that frequent them, especially over the summer. Landslide susceptibility of the cliff has been assessed using the Rock Engineering System method (RES). The determinant and triggering factors considered in this study include: wave exposure, shoreline variations, cliff height, cliff slope, geotechnical quality of the rocky mass, superficial runoff and cliff orientations favoring landslides. Geographic Information Systems (GIS) have been employed to facilitate the information analysis and generate new susceptibility maps. The quality of the rock mass and cliff orientation are the most interactive factors for the stability of the cliff. However, shoreline variations and surface runoff are the most dominant factors in the system. Thus, the quality of the rock mass has been determined to be a basic variable in the cliff characterization because of its high dependence on the variations of the remaining factors. The landslide susceptibility map depicts a predominance of surfaces with moderate degrees of susceptibility concentrated mainly in the headlands, where the combined actions of subaerial and marine processes control the weathering and eroding processes. Therefore, the landslide susceptibility assessment based on this methodology has allowed the identification of hazardous areas that should be considered in future management plans

Characterization of goat colostrum oligosaccharides by nano-liquid chromatography on chip quadrupole time-of-flight mass spectrometry and hydrophilic interaction liquid chromatography-quadrupole mass spectrometry

A detailed qualitative and quantitative characterization of goat colostrum oligosaccharides (GCO) has been carried out for the first time. Defatted and deproteinized colostrum samples, previously treated by size exclusion chromatography (SEC) to remove lactose, were analyzed by nanoflow liquid chromatography–quadrupole-time of flight mass spectrometry (Nano-LC-Chip–Q-TOF MS). Up to 78 oligosaccharides containing hexose, hexosamine, fucose, N-acetylneuraminic acid or Nglycolylneuraminic acid monomeric units were identified in the samples, some of them detected for the first time in goat colostra. As a second step, a hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) methodology was developed for the separation and quantitation of the main GCO, both acidic and neutral carbohydrates. Among other experimental chromatographic conditions, mobile phase additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry of target carbohydrates. Narrow peaks (wh: 0.2–0.6 min) and good symmetry (As: 0.8–1.4) were obtained for GCO using an acetonitrile:water gradient with 0.1% ammonium hydroxide at 40 ◦C. These conditions were selected to quantify the main oligosaccharides in goat colostrum samples. Values ranging from 140 to 315 mg L−1 for neutral oligosaccharides and from 83 to 251 mg L−1 for acidic oligosaccharides were found. The combination of both techniques resulted to be useful to achieve a comprehensive characterization of GCO.This work has been funded by Junta de Andalucía (project AGR2011-7626), CSIC (project i-link0827), Comunidad Autónoma de Madrid (Spain) and European funding from FEDER program (AVANSECAL-CM S2013/ABI-3028) and Fundación Ramón Areces. This work was also supported by the UC Davis RISE program and the National Institutes of Health awards R21AT006180, R01AT007079, R01AT008759-02.Peer reviewe

The Portuguese grapevine cultivar 'Amaral': synonymies, homonymies and misnames

Within Western Europe, Portugal is the country with highest diversity of grapevine cultivars. As the Iberian Peninsula has been considered a domestication centre of grapevines, the interest on Portuguese grapevine cultivars diversity has increased in the last years.Using twelve SSR markers scattered across twelve grape chromosomes, thirty-three accessions collected on Vinhos Verdes and Lafões Controlled Designations of Origin (DOC) and supposedly of the grapevine cultivar 'Amaral', were analysed. Names of the accessions ranged from 'Amaral' and 'Azal Tinto' to 'Sousão Galego'. Eight different genotypes were detected. The majority of accessions had the genotype attributed to 'Amaral'. However, three accessions were identified as misnaming cases and four genotypes were considered unknown, as no cultivar identification was achieved. Nevertheless, not only the misnamed cultivars, 'Vinhão', 'Melhorio' and 'Touriga Nacional', but also the four unknown genotypes, 'Azal Tinto' (AT2203 and AT2206), 'Amaral-1', 'Amaral-2' and 'Sousão Galego', share at least one allele in each of the 12 SSR loci analysed.The accessions AT2203 and AT2206 were found as possible offspring of 'Amaral' and 'Folgasão' and the accession 'Amaral-2' is likely result of a cross between 'Amaral' and 'Pedral'.The high number of synonym names that are attributed to 'Amaral', like 'Azal Tinto', 'Azar', 'Cainho' or 'Susão Galego' and the fact that it probably has parent-offspring relations with many cultivars, suggests that 'Amaral' is an ancient cultivar in the Northwest of the Iberian Peninsula and was a very important cultivar in the past.

Identification of minority grapevine cultivars from Vinhos Verdes Portuguese DOC Region

The Portuguese Vinhos Verdes DOC Region retains a considerable biodiversity in grapevine varieties. Unfortunately, regional grapevine patrimony is in danger of disappearing. The present study was carried out in order to genotype, using 12 microsatellite loci, a group of 39 accessions of Vinhos Verdes DOC region, including underuse and minor varieties included in the wine list of varieties and also several unknown accessions with regional designations. The accessions analyzed were identified and grouped into 34 different genotypes, nine of them referred as new genotypes. Some new synonyms were detected, namely between Spanish and Portuguese cultivars. Misidentifications and wrong designations were also detected

Characterization of grapevine (Vitis vinifera L.) cultivars from northern Portugal using RAPD and microsatellite markers

Twelve grapevine cultivars from northern Portugal were characterized with RAPD and microsatellites. Nine primers were used in the RAPD analysis; 8 of the varieties showed monotypic patterns. With the 6 microsatellite loci a total of 38 alleles was obtained. Relationships among the studied varieties were observed. Both types of molecular markers have proved useful for identification. Existence of synonymies is discussed.

Prospection and identification of grapevine varieties cultivated in north Portugal and northwest Spain

A broad prospection of grapevine plants was carried out in northwest Spain and north of Portugal during the period from 2002 to 2009. It included zones located in Galicia as well as in the provinces of León, Zamora and Salamanca in Spain and the region delimited by the right side of the Douro river and the spanish border in Portugal. A total of 669 accessions were sampled, studied and identified, both by using the six microsatellite loci included in the OIV descriptors list, plus ampelographic characters. As a result of the study a total of 53 different grapevine varieties were identified. Most of them correspond to minor varieties that are present in the zone. In many cases the same variety was detected in both countries, although denominated with different names that are synonymous. The molecular markers that were analyzed are those included in the OIV 801 to 806 descriptors, and correspond to the following loci: VVS2, VVMD5, VVMD7, VVMD27, ssrVrZAG62 and ssrVrZAG79. Allele sizes of the studied varieties are recorded. Relationships among these varieties seem to be possible. Recommended priority names for the studied varieties both in Spain and in Portugal are listed, in order to avoid misidentifications and reduce the incidence of homonymies.

A genomics approach identifies selective effects of trans-resveratrol in cerebral cortex neuron and glia gene expression

The mode of action of trans-resveratrol, a promising lead compound for the development of neuroprotective drugs, is unknown. Data from a functional genomics study were retrieved with the aim to find differentially expressed genes that may be involved in the benefits provided by trans-resveratrol. Genes that showed a significantly different expression (p<0.05, cut-off of a two-fold change) in mice fed with a control diet or a control diet containing trans-resveratrol were different in cortex, heart and skeletal muscle. In neocortex, we identified 4 up-regulated (Strap, Pkp4, Rab2a, Cpne3) and 22 down-regulated (Actn1, Arf3, Atp6v01, Atp1a3, Atp1b2, Cacng7, Crtc1, Dbn1, Dnm1, Epn1, Gfap, Hap, Mark41, Rab5b, Nrxn2, Ogt, Palm, Ptprn2, Ptprs, Syn2, Timp2, Vamp2) genes upon trans-resveratrol consumption. Network analysis of gene products provided evidence of plakophilin 4 up-regulation as a triggering factor for down-regulation of events related to synaptic vesicle transport and neurotransmitter release via underexpression of dynamin1 and Vamp2 (synaptobrevin 2) as node-gene drivers. Analysis by RT-qPCR of some of the selected genes in a glioma cell line showed that dynamin 1 mRNA was down-regulated even in acute trans-resveratrol treatments. Taken all together, these results give insight on the glial-neuronal networks involved in the neuroprotective role of trans-resveratrol

Educación Deportiva versus Enseñanza Tradicional: Influencia sobre la regulación motivacional en alumnado de Bachillerato

El objetivo de esta investigación fue analizar el impacto de una intervención basada en el Modelo de Educación Deportiva (MED), en comparación con el Modelo de Enseñanza Tradicional (MET), sobre la regulación motivacional en alumnado de bachillerato en clase de Educación Física. Participó un total de 44 estudiantes (22 hombres y 22 mujeres, Medad = 16.32, DT = 0.57) de bachillerato. El diseño fue un estudio cuasi-experimental con medidas pre- y post-test y comparaciones intra- e inter-grupo. El programa de intervención consistió en 12 sesiones para la enseñanza del baloncesto, siguiéndose las directrices recomendadas por la literatura tanto para el grupo MED como para el grupo MET. Los resultados han indicado que el MED, respecto al MET, mejoró signicativamente el nivel de motivación intrínseca y de regulación identicada. Los hallazgos también han mostrado como el MED redujo signicativamente el nivel de regulación externa y desmotivación comparado con el MET. Como conclusión, el MED es un modelo de enseñanza deportiva que favorece el desarrollo de las regulaciones de motivación más autodeterminadas (motivación intrínseca y regulación identicada) en el proceso de enseñanza-aprendizaje en el contexto escolar, lo que podría suscitar el interés en el alumnado por la práctica deportiva de modo regular en el tiempo libre.