Dissolved matter fluxes in the inner delta of the River Niger

Abstract This study is part of the EQUANIS programme, the objectives of which are (a) estimating inputs from the River Niger to the inner Delta; (b) computing the hydrological balance; and (c) monitoring the quality of water in the central lacustrine basin. Eleven sampling locations were selected near gauging stations, both on the Niger River and its main tributary, the Bani River. Those sites have been sampled weekly since July 1990. The inner delta of the Niger River is a system particularly subject to sahelian and sub-desert climatic conditions and characterized by large flood plains. Time series of input water volumes in the inner delta, and of the water losses within it, show that the water losses are high, due to the intense evaporation, and vary from 6 to 40 km3. The water .losses reach their maximum during the wettest years-up to 47%, and minimum during the driest years-only 32%, due to the reduction of the flooded area. The surface of the flooded area is inferred from the hydrological balance. The preliminary results of this study indicate that the Niger and Bani rivers have low levels of dissolved element concentrations. The mean conductivity values, ranging from 50-80 pS cm-', increase regularly during the low water stage and decrease drastically with rising water. The pH values are slightly basic, ranging from 7.1 to 8. Silica and bicarbonates are the main dissolved species; they always represent more than 75% of TDS. In May, when the rising water stage begins, the waters are poorly mineralized at the input of the delta, while they have been enriched during the dry season in the delta. A good mass balance is found between inputs and outputs through the delta. However, a disequilibrium appears at the sampling sites within the basin, which could be partially linked to poor mixing between the Niger and Bani river waters. The first results from the upper basin and below the inner delta, show low concentrations of matter. The specific dissolved loads vary between 10-12 t km-' year-' for the Niger River and 2.5 t km-2 year-' for the Bani River. The annual input in the inner delta was about 2.2 X lo6 t in 1992-1993. Chemical budgets show a saline deposit of 0.3 x lo6 t in the inner delta. Seasonal variations of the dissolved matter fluxes are very different between the upper and lower parts of the inner delta, due to the breaking of the annual flood and to the more important flood plains in the upper delta. Los flujos de materiales disueltos en el delta inerior del Niger Resumen Es presente estudio forma parte del programa EQUANIS, cuyos objetivos son (a) estimación de los aportes del río Niger al delta interior, (b) cómputo del balance gídrico, y (c) monitoreo de la calidad del agua en la cuenca lacustre central. Se seleccionaron once sitios de muestre0 próximos a las estaciones de aforo tanto en el Niger como en su principal afluente, el Bani. Desde julio de 1990 se llevaron a cabo allí muestreos semanales. EI delta interior del Niger is us sistema particular sometido a condiciones interior, y de pérdidas de agua en is mismo, muestran que las pérdidas son elevadas debido a la evaporación intensa, y fluctúan entre 40 y 6 km3. Las pérdidas de agua son máximas durante los años más húmedos (hasta un 47%) y mínimas durante los años más socos (solo 32%) debido a la reducción de las Breas inundadas. La superficie inundada se deduce del balance hídrico. Los resultados preliminares del estudio indican que los ríos Niger y Bani tienen un bajo nivel de concentración de elementos en disolución. Los valores medios de conductividad que van de 50 a 80 pS cm" se incrementan regularmente durante el período de estiaje y disminuyen drásticamente en períodos de avenidas y variacíon de niveles. Los valores de pH son ligeramente básicos, de 7.1 a 8. Los principales materiales dusueltos son el sílice y los bicarbonatos; siempre representan más del 75% del TDS. En mayo, cuando se inicia el período de crecida, las aguas contienen pocos minerales en la entrada del delta mientras que se han concentrado durante la estación seca del delta. Se observa un buen balance de masa en el delta entre los aportes y descargas, pero aparece un desequilibrio en los sitios de muestre0 dentro de la cuenca, que puede atribuirse parcialmente a una mala mezcla entre las aguals del Niger y del Bani. Los primeros resultados en la cuenca superior y baja del delta interior muestran bajas concentraciones de material. Las cargas disueltas específicas fluctúan entre 10 y 12 t km-2 año-' para el río Niger y 2.5 t km-* año-' para el río Bani. El aporte anual en el delta interior fue de unos 2.2 X lo6 t en 1992-1993. Los balances químicos presentan un depósito salino de 0.3 X lo6 t en el delta interior. Las variaciones estacionales de los flujos de materiales son muy diferentes entre las partes alta y baja del delta interior, debido al inicio de la crecida anual y a las más importantes planicies de inundación en la parte superior del delta

Between a rock and a hard place: Associations between Mentzos' “dilemma”, self‐reported interpersonal problems, and psychosocial functioning in individuals with non‐affective psychoses

Primary aim of this study was to determine the extent and type of self-reported interpersonal problems in patients with non-affective psychoses and their impact on psychosocial functioning. Furthermore, we aimed to explore potential links with the psychodynamic construct of Stavros Mentzos' "psychotic dilemma", which describes an insufferable inner tension caused by an individual's struggle of being torn between "self-oriented" and "object-oriented" tendencies. In a cross-sectional study among 129 patients with non-affective psychoses, measures of cognition, symptom load and social functioning as well as a tentative, psychodynamic assessment of Mentzos' "dilemma" were obtained during a clinical research visit. Self-report data on interpersonal problems were gathered using the Inventory of Interpersonal Problems (IIP-64D) and compared with a German representative standard sample. Second, IIP-64D scores were compared between groups with or without Mentzos' "dilemma". Hierarchical regression analyses were performed to test for the impact of interpersonal problems on psychosocial functioning, while controlling for cognitive deficits and psychopathology. Results showed that IIP-64D scores differed significantly from healthy controls, except for "self-centred" and "intrusive" interpersonal styles. Participants with a potential "psychotic dilemma" scored significantly higher on the subscales: "domineering", "self-centred", "cold", and "socially avoidant" than the group without a "psychotic dilemma". The total amount of interpersonal problems, and particularly high scores on the IIP-64D "socially avoidant" subscale, predicted psychosocial dysfunction, whereas a "cold" interpersonal style had an opposite effect. In conclusion, specific interpersonal problems may predict psychotherapeutic outcome measures like psychosocial functioning and are partly compatible with the psychodynamic construct of Stavros Mentzos' "psychotic dilemma"

Extracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in Pseudomonas aeruginosa tobramycin-enhanced biofilm formation

Biofilms are structured microbial communities that are the leading cause of numerous chronic infections which are difficult to eradicate. Within the lungs of individuals with cystic fibrosis (CF), Pseudomonas aeruginosa causes persistent biofilm infection that is commonly treated with aminoglycoside antibiotics such as tobramycin. However, sublethal concentrations of this aminoglycoside were previously shown to increase biofilm formation by P. aeruginosa, but the underlying adaptive mechanisms still remain elusive. Herein, we combined confocal laser scanning microscope analyses, proteomics profiling, gene expression assays and phenotypic studies to unravel P. aeruginosa potential adaptive mechanisms in response to tobramycin exposure during biofilm growth. Under this condition, we show that the modified biofilm architecture is related at least in part to increased extracellular DNA (eDNA) release, most likely as a result of biofilm cell death. Furthermore, the activity of quorum sensing (QS) systems was increased, leading to higher production of QS signaling molecules. We also demonstrate upon tobramycin exposure an increase in expression of the PrrF small regulatory RNAs, as well as expression of iron uptake systems. Remarkably, biofilm biovolumes and eDNA relative abundances in pqs and prrF mutant strains decrease in the presence of tobramycin. Overall, our findings offer experimental evidences for a potential adaptive mechanism linking PrrF sRNAs, QS signaling, biofilm cell death, eDNA release, and tobramycin-enhanced biofilm formation in P. aeruginosa. These specific adaptive mechanisms should be considered to improve treatment strategies against P. aeruginosa biofilm establishment in CF patients’ lungs

Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-Resolution Microscopy

Super-resolution 3D imaging reveals remodeling of the cortical actin meshwork at the natural killer cell immune synapse, which is likely to be important for secretion of lytic granules

Engineering access to higher education through higher education fairs

Text from van Zanten A., Legavre A. “Engineering access to higher education through higher education fairs”, in Goastellec G., Picard F. (ed.) The Roles of Higher Education and Research in the Fabric of Societies, Leuven, Sense Publishers, 2014 (in press). Transition to higher education is a major social process. This transition has been mostly studied by French sociologists of education and higher education from perspectives focusing predominantly on the role of the socio-economic status, academic profiles and different tracks followed by secondary school students (Merle 1996, Duru-Bellat and Kieffer 2008, Convert 2010), and, to a lesser extent, on the types of secondary schools attended (Duru-Bellat and Mingat 1998, Nakhili 2005) and the local higher education provision (Berthet et al. 2010, Orange 2013). Although these structural determinants play a major role in explaining significant regularities, they provide more powerful explanations for individuals representing the extremes of the different variables considered, leaving room for the influence of other major factors for those students in intermediate situations. In addition, even in the case of students occupying extreme positions, structural perspectives better explain the distribution of students between different higher education tracks than they do between institutions and disciplines. In this chapter, we adopt a perspective that we see as complementary to and interacting with the perspective centred on structural determinants by focusing on the role of the devices that mediate the exchanges between students and higher education institutions, and more specifically on one device: higher education fairs. Our purpose in doing so is not only to document how these various devices frame, in ways that remain largely unexplored by researchers, exchanges between providers and consumers of higher education but also to point out – and further explore in future publications – how these devices, and the specific features of fairs, contribute to the reproduction and transformation of educational inequalities in access to higher education (Benninghoff et al. 2012)

Comparative analysis of the lambda-interferons IL-28A and IL-29 regarding their transcriptome and their antiviral properties against hepatitis C virus.

Specific differences in signaling and antiviral properties between the different Lambda-interferons, a novel group of interferons composed of IL-28A, IL-28B and IL-29, are currently unknown. This is the first study comparatively investigating the transcriptome and the antiviral properties of the Lambda-interferons IL-28A and IL-29. Expression studies were performed by microarray analysis, quantitative PCR (qPCR), reporter gene assays and immunoluminometric assays. Signaling was analyzed by Western blot. HCV replication was measured in Huh-7 cells expressing subgenomic HCV replicon. All hepatic cell lines investigated as well as primary hepatocytes expressed both IFN-λ receptor subunits IL-10R2 and IFN-λR1. Both, IL-28A and IL-29 activated STAT1 signaling. As revealed by microarray analysis, similar genes were induced by both cytokines in Huh-7 cells (IL-28A: 117 genes; IL-29: 111 genes), many of them playing a role in antiviral immunity. However, only IL-28A was able to significantly down-regulate gene expression (n = 272 down-regulated genes). Both cytokines significantly decreased HCV replication in Huh-7 cells. In comparison to liver biopsies of patients with non-viral liver disease, liver biopsies of patients with HCV showed significantly increased mRNA expression of IL-28A and IL-29. Moreover, IL-28A serum protein levels were elevated in HCV patients. In a murine model of viral hepatitis, IL-28 expression was significantly increased. IL-28A and IL-29 are up-regulated in HCV patients and are similarly effective in inducing antiviral genes and inhibiting HCV replication. In contrast to IL-29, IL-28A is a potent gene repressor. Both IFN-λs may have therapeutic potential in the treatment of chronic HCV

Natural Killer Cell Signal Integration Balances Synapse Symmetry and Migration

Imaging immune surveillance by natural killer (NK) cells has revealed that integration of activating and inhibitory signals determines whether or not NK cells stop to kill the target cell or retain a migratory configuration

Lentiviral Hematopoietic Stem Cell Gene Therapy in Patients with Wiskott-Aldrich Syndrome.

iskott-Aldrich syndrome (WAS) is an inherited immunodeficiency caused by mutations in the gene encoding WASP, a protein regulating the cytoskeleton. Hematopoietic stem/progenitor cell (HSPC) transplants can be curative, but, when matched donors are unavailable, infusion of autologous HSPCs modified ex vivo by gene therapy is an alternative approach. We used a lentiviral vector encoding functional WASP to genetically correct HSPCs from three WAS patients and reinfused the cells after a reduced-intensity conditioning regimen. All three patients showed stable engraftment of WASP-expressing cells and improvements in platelet counts, immune functions, and clinical scores. Vector integration analyses revealed highly polyclonal and multilineage haematopoiesis resulting from the gene-corrected HSPCs. Lentiviral gene therapy did not induce selection of integrations near oncogenes, and no aberrant clonal expansion was observed after 20 to 32 months. Although extended clinical observation is required to establish long-term safety, lentiviral gene therapy represents a promising treatment for WAS