Hepatoxicity of aqueous extract and fractionated methanol extract of Phytolacca americana by isolated rat liver perfusion system

Recombinant retroviral vector containing human tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) gene was constructed and investigation of the in vitro invasion and metastasis of gastric cancer cells transfected with TIMP-2 was carried out. Human TlMP-2 was isolated from recombinant vector Bluescript 1/TIMP-2(+), and then inserted into the retroviral vector pL-MT. Correct orientation was verified by restriction endonuclease digestion. Human full length TIMP-2 gene was ligated into a plasmid, which was then transfected into PA317 cell line. G418-resistant individual clones were selected to transfect human SGC-7901 cell line. Cell proliferation, cell electrophoresis, soft agar colony formation and in vitro invasion were detected to analyze the bio-behavioral changes of cancer cells. The results from restriction endonuclease digestion were as theoretically expected. The cell electrophoresis rate, colony number and invasion ability in SGC-7901 cells and MFC cells transfected with TIMP-2 gene were significantly decreased when compared with control group. However, no significant changes were noted in the proliferation of cancer cells. We successfully construct a recombinant retroviral vector containing human TIMP-2. TIMP-2 transfection could markedly alter the membrane charge of cancer cells, resulting in decreased electrophoresis capacity, cell migration and invasion. However, cell growth was not affected by TIMP-2. These results suggested TIMP-2 transfection might exert effects on the malignant phenotype of cancer cells through affecting extracellular environment, which provided a new way to investigate gene regulation of in vitro collagen metabolism

Possible spin-orbit driven spin-liquid ground state in the double perovskite phase of Ba3YIr2O9

We report the structural transformation of hexagonal Ba3YIr2O9 to a cubic double perovskite form (stable in ambient conditions) under an applied pressure of 8GPa at 1273K. While the ambient pressure (AP) synthesized sample undergoes long-range magnetic ordering at 4K, the high pressure(HP) synthesized sample does not order down to 2K as evidenced from our susceptibility, heat capacity and nuclear magnetic resonance (NMR) measurements. Further, for the HP sample, our heat capacity data have the form gamma*T+beta*T3 in the temperature (T) range of 2-10K with the Sommerfeld coefficient gamma=10mJ/mol-Ir K2. The 89Y NMR shift has no T-dependence in the range of 4-120K and its spin-lattice relaxation rate varies linearly with T in the range of 8-45K (above which it is T-independent). Resistance measurements of both the samples confirm that they are semiconducting. Our data provide evidence for the formation of a 5d based, gapless, quantum spin-liquid (QSL) in the cubic (HP) phase of Ba3YIr2O9. In this picture, the T term in the heat capacity and the linear variation of 89Y 1/T1 arises from excitations out of a spinon Fermi surface. Our findings lend credence to the theoretical suggestion [G. Chen, R. Pereira, and L. Balents, Phys. Rev. B 82, 174440 (2010)] that strong spin-orbit coupling can enhance quantum fluctuations and lead to a QSL state in the double perovskite lattice.Comment: 6 pages 5 figure

Effect of Metformin on Serum Ferritin Level in Women with Polycystic Ovary Syndrome

Background: Polycystic ovary syndrome (PCOS) is one of the most common diseases among women associated with various inflammatory reactants such as C-reactive protein (CRP) and ferritin. This study aimed to investigate the effect of metformin on probable reduction of serum ferritin in patients with PCOS.Methods: This study was conducted on 45 patients with PCOS who had not other systemic diseases and did not take any medications. Weight, waist and hip circumstances (WHR), body mass index (BMI), metabolic indexes,CRP, ferritin and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) double acute accent were measured before the study. Metformin (500 mg/tid) tablets were prescribed for three months and then same above parameters were re-measured.Results: Of 45 patients, 19 (42.2) were overweight and 14 (31.1) were obese. After drug therapy, there was a significant reduction in waist circumstance and serum ferritin. This reduction was significant only in the lean and overweight groups but not in the obese group. There was not significant association between serum ferritin and CRP, HOMA-IR, BMI and WHR. There was not significant correlation between CRP and HOMA-IR and also BMI.Conclusion: The effect of metformin on reduction of serum ferritin was not significant just in obese group and was not associated with metabolic and anthropometric indexes. © Iranian Red Crescent Medical Journal

Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria

A decadally delayed response of the tropical Pacific to Atlantic multidecadal variability

North Atlantic sea surface temperature anomalies are known to affect tropical Pacific climate variability and El Niño-Southern Oscillation (ENSO) through thermocline adjustment in the equatorial Pacific Ocean. Here coupled climate simulations featuring repeated idealized cycles of the Atlantic Multidecadal Oscillation (AMO) generated by nudging its tropical branch demonstrate that the tropical Pacific response to the AMO also entails a substantial decadally delayed component. The simulations robustly show multidecadal fluctuations in central equatorial Pacific sea surface temperatures lagging the AMO by about three decades and a subdecadal cold-to-warm transition of the tropical Pacific mean state during the AMO's cooling phase. The interplay between out-of-phase responses of seawater temperature and salinity in the western Pacific and associated density anomalies in local thermocline waters emerge as crucial factors of remotely driven multidecadal variations of the equatorial Pacific climate. The delayed AMO influences on tropical Pacific dynamics could help understanding past and future ENSO variability.North Atlantic sea surface temperature anomalies are known to affect tropical Pacific climate variability and El Niño-Southern Oscillation (ENSO) through thermocline adjustment in the equatorial Pacific Ocean. Here coupled climate simulations featuring repeated idealized cycles of the Atlantic Multidecadal Oscillation (AMO) generated by nudging its tropical branch demonstrate that the tropical Pacific response to the AMO also entails a substantial decadally delayed component. The simulations robustly show multidecadal fluctuations in central equatorial Pacific sea surface temperatures lagging the AMO by about three decades and a subdecadal cold-to-warm transition of the tropical Pacific mean state during the AMO's cooling phase. The interplay between out-of-phase responses of seawater temperature and salinity in the western Pacific and associated density anomalies in local thermocline waters emerge as crucial factors of remotely driven multidecadal variations of the equatorial Pacific climate. The delayed AMO influences on tropical Pacific dynamics could help understanding past and future ENSO variability. © 2016. American Geophysical Union. All Rights Reserved

The factor structure of the mood disorder questionnaire in Tunisian patients

Background: The Mood Disorder Questionnaire (MDQ) is a frequently used screening tool for the early detection of Bipolar Disorder (BD), which is often unrecognized or misdiagnosed at its onset. In this study, data from Tunisia has been used to evaluate the psychometric properties of the Arabic MDQ. Methods: The sample included 151 patients with a current major depressive episode. The Arabic adapted version of the Structured Clinical Interview for DSM-IV-TR was used to formulate a diagnosis, yielding 62 patients with BD and 89 with unipolar Major Depressive Disorder (MDD). Principal component analysis with parallel analysis was used to establish the spontaneous distribution of the 13 core items of the MDQ. Confirmatory Factor Analysis (CFA) was used to check the available factor models. Receiver Operating Characteristic (ROC) analysis was used to assess the capacity of the MDQ to distinguish patients with BD from those with MDD. Results: Cronbach’s α in the sample was 0.80 (95%CI: 0.75 to 0.85). Ordinal α was 0.88. Parallel analysis suggested two main components, which explained 59% of variance in the data. CFA found a good fit for the existing unidimensional, the two-factor, and the three-factor models. ROC analysis showed that at a threshold of 7, the MDQ was able to distinguish patients with BD from those with MDD with extraordinary negative predictive value (0.92) and a positive diagnostic likelihood ratio of 3.8. Conclusion: The Arabic version of the MDQ showed good measurement properties in terms of reliability, factorial validity and discriminative properties

Accuracy of the Arabic HCL - 32 and MDQ in detecting patients with bipolar disorder

Background: Studies about the two most used and validated instruments for the early detection of Bipolar Disorder (BD), the 32 - item Hypomania Checklist (HCL - 32) and the Mood Disorder Questionnaire (MDQ), are scarce in non-Western countries. This study aimed to explore the reliability, factor structure, and criterion validity of their Arabic versions in a sample of Tunisian patients diagnosed with mood disorders. Methods: The sample included 59 patients with BD, 86 with unipolar Major Depressive Disorder (MDD) and 281 controls. Confirmatory factor analysis was applied to show that a single global score was an appropriate summary measure of the screeners in the sample. Receiver Operating Characteristic analysis was used to assess the capacity of the translated screeners to distinguish patients with BD from those with MDD and controls. Results: Reliability was good for both tools in all samples. The bifactor implementation of the most reported two-factor model had the best fit for both screeners. Both were able to distinguish patients diagnosed with BD from putatively healthy controls, and equally able to distinguish patients diagnosed with BD from patients with MDD. Conclusion: Both screeners work best in excluding the presence of BD in patients with MDD, which is an advantage in deciding whether or not to prescribe an antidepressant

Rational design of hypoallergenic vaccines: Blocking ige-binding to polcalcin using allergen-specific igg antibodies

Chenopodium album polcalcin (Che a 3) is characterized as a major cause of cross-reactivity inallergic patients to the Chenopodiaceae family. Therefore, the present study was conducted to develop a hypoallergenic Che a 3 derivatives as the candidate vaccine for type 1 allergy. Four derivatives were generated from Che a 3. The first was a mosaic peptide derivative computationally identified in Che a 3 which was coupled to keyhole limpet hemocyanin (KLH). The second one was a mutant Che a 3, and the other two derivatives included N-and C-Terminal halves of Che a 3 that both coupled to KLH. The IgE-binding capacity of Che a 3 and its derivatives and also their ability to induce there combinant Che a 3 (rChe a 3)-specific IgG antibody, were determined using the enzyme-linked immune sorbent assay (ELISA). Moreover, the lymphopro liferative capacity of rChe a 3 or its derivatives and their pro-inflammatory cytokine response interleukin (IL)-5 and IL-13 were measured in the human peripheral blood mononuclear cells (PBMCs). Among all derivatives, the N-Terminal half peptide and mosaic peptide exhibited the lowest IgEbinding capacity. In addition, in comparison to other antigens, KLH-coupled mosaic peptide induced the highest level of the recombinant Che a 3 (rChe a 3)-specific IgG antibody and ther Che a 3 specific-blocking IgG antibody in mice. Moreover, the mosaic peptide lacked lymphopro liferative capacity and down-regulated expression of pro-Allergic IL-5 and IL-13 cytokines. Therefore, a peptide-carrier fusion vaccine, composed of the B-cell epitope coupled to the carrier, could be considered as one of the promising hypoallergenic vaccines to treat patients with allergy to low molecular weight allergens such as Che

Investigation about the effect of manufacturing parameters on the mechanical behaviour of natural fibre nonwovens reinforced thermoplastic composites

To date, nonwoven fabrics made with natural fibres and thermoplastic commingled fibres have been extensively used in the composite industry for a wide variety of applications. This paper presents an innovative study about the effect of the manufacturing parameters on the mechanical behaviour of flax/PP nonwoven reinforced composites. The mechanical properties of nonwoven fabric reinforced composites are related directly to the ones of dry nonwoven reinforcements, which depend strongly on the nonwoven manufacturing parameters, such as the needle-punching and areal densities. Consequently, the influence of these manufacturing parameters will be analysed through the tensile and flexural properties. The results demonstrated that the more areal density the nonwoven fabric has, the more the mechanical behaviour can be tested for composites. By contrast, it has a complex influence on needle-punching density on the load-strain and bending behaviours at the composite scale