Tactical urbanism as a catalyst for democratic urban spaces

Ponència presentada a: Session 8: Dimensiones psicosociales de la arquitectura y el urbanismo / Psycological dimensions of architecture and plannin

Pulmonary mycobiome of patients with suspicion of respiratory fungal infection – an exploratory study

Objective: This pilot study aimed to characterize the pulmonary mycobiome of patients with suspicion of fungal infection of the respiratory tract as well as to identify potentially pathogenic fungi colonizing/infecting their lungs. Methods: A cohort of 10 patients was analyzed, including HIV+ patients and patients with active infection caused by Mycobacterium species. Their respiratory samples (bronchoalveolar lavage fluid/ bronchial secretions) were pre-treated with lyticase and proteinase K; DNA was extracted using the High Pure PCR Template Preparation kit following the manufacturer’s instructions. The internal transcribed spacer region 1 (ITS1) and calmodulin gene were amplified by PCR and the resulting amplicons were sequenced using the Illumina MiSeq platform with pair-end reads of 150 bp. The obtained results were analyzed using the PIPITS pipeline as described by Gweon et al. [1]. Operational taxonomic units (OTU) to which less than 0.1% of the total reads attributed were disregarded. Results: Thirty-seven different OTU were identified from which two belonged to the Plantae kingdom, 11 had less than the 0.1% threshold of the total reads and were therefore disregarded. The remaining 24 different OTU (grouped in 17 phylotypes), were considered as part of the pulmonary mycobiome of patients. Two phyla were identified: Basidiomycota (33.3%) and Ascomycota (54.2%). Regarding the Basidiomycota phylum, reads were classified in three classes (Agaricomycetes, Tremellomycetes and Walleomycetes), while for the Ascomycota phylum four different taxonomical classes were identified: Pneumocystidomycetes, Dothideomycetes, Eurotiomycetes and Saccharomycetes, with the latter being the most frequent class. Twelve fungal genera were identified, being Candida the most frequently detected. The median number of fungal genera detected in patients’ pulmonary mycobiome was six (ranging from two up to nine). The genus Papilotrema and the potentially pathogenic genera Cryptococcus and Pneumocystis were exclusively found in the pulmonary mycobiome of HIV+ + patients. Other potentially pathogenic fungi such as Aspergillus spp., Trichosporon spp., Saccharomyces spp. and Schizophyllum spp. were also detected. Conclusion: This pilot study illustrates how the pulmonary mycobiome is rich and highly variable in patients with fungal infections. The obtained results suggest that the described metagenomic analysis may possess a great ability to quickly and effectively detect potentially pathogenic fungi in the mycobiome of patients, making it a promising future diagnostic tool. Thus, further optimization, standardization and clinical validation of these NGS methodologies should be warranted in the future.info:eu-repo/semantics/publishedVersio

Para a caracterização da disciplina de Língua Portuguesa no Ensino Básico

Pretende-se, neste estudo, identificar o estatuto que a disciplina de Língua Portuguesa tem no currículo do Ensino Básico, estabelecendo como ponto de referência a Reforma do Sistema Educativo iniciada em 1986. Porém, e uma vez que nos encontramos num período em que estão em curso algumas medidas de reorganização curricular, não poderemos deixar de fazer referência às concepções que as atravessam. Por fim, conscientes de que as justificações do seu estatuto não podem apenas ser encontradas na nossa sincronia, procuraremos na história, de forma necessariamente sucinta, elementos que nos ajudem a perceber a importância hoje atribuída à disciplina

Estatuto e funções do manual escolar de língua Portuguesa

Na tentativa de identificação do estatuto de que se revestem os manuais escolares no processo de ensino-aprendizagem, estabelecemos, como linha de orientação neste capítulo, a caracterização das suas funções com base em i) concepções teóricas, ii) discursos oficiais e iii) nas práticas lectivas. Consideramos, numa análise inicial, o estatuto e funções do manual escolar em geral, para, posteriormente, nos referirmos ao manual de Língua Portuguesa

Gramática no ensino básico - renascer do caos ou o extermínio do mito?

No momento em que se assiste à tentativa de afirmação pedagógica da Terminologia Linguística para os Ensinos Básico e Secundário (TLEBS), muitas vozes fazem eco dos seus pareceres. Uns baseiam-se em critérios científicos para justificar os seus argumentos pró ou contra; outros, em critérios de natureza mais didáctica. Afinal, trata-se de romper com princípios e práticas enraizados numa lógica gramatical justificada pela tradição; a nomenclatura gramatical presente nos Programas é ainda a definida por Portaria do MEN, publicada no Diário do Governo, 1ª série, de 28 de Abril de 1967. Esta tentativa de colocar um estado de ordem no ensino-aprendizagem da gramática, leia-se uniformizar conceitos, surge já no texto que se apresenta como orientador das práticas lectivas, no que à disciplina de Língua Portuguesa diz respeito: A Língua Materna na Educação Básica (LMEB). Neste documento, que numa fórmula “pronto-a-usar” se condensou num livro conhecido no âmbito pedagógico como o livro das Competências, é referida a utilização da então nova Proposta de Nomenclatura Gramatical apresentada por DUARTE et al. (1991). (Cf. SIM-SIM et al.,1997,p. 84). Em consequência, alguns manuais tentaram actualizar as propostas de análise gramatical tendo por base esta nova terminologia, fazendo desde logo adivinhar a multiplicidade de noções e conceitos que passaram a emergir dos manuais (Cf. Pinto, 2000)

Study of a 9th century silver earrings set from Mikulčice: corrosion, conservation and maintenance

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Conservação e RestauroThe presented study deal with the degradation processes of the silver objects, buried for a long time in human graves and with their appropriate methods of conservation and restoration after excavation during archaeological research. It was used for this study a set of earrings from the 9th century A.D. found in Mikulčice, in the southeast of Czech Republic. To understand the mechanisms and process of corrosion and the manufacture and decoration techniques of the earrings was performed: structural study by photo-documentation in the optic stereo-microscope and by radiography, and microstructure and elemental analysis with scanning electron microscopy with energy dispersive X-ray (SEM-EDS). It was also made detailed metallographic analysis of one pure silver earring fragment to study manufacture technique and the buried silver corrosion process. Related to silver earrings set maintenance conditions was study the atmospheric corrosion of silver in museum environments. For that, it was measure the corrosion rate and film thickness by galvanostatic reduction technique, of 15 silver samples placed in exhibition and storage rooms from the National Museum and Museum of Decorative Arts of Prague