Renal sympathetic ablation using an irrigated-tip catheter: an attractive option?

BACKGROUND: Catheter-based renal sympathetic denervation has emerged as an adjunct strategy to control refractory hypertension. No studies have yet compared the tissue effects of different catheters, powers and time periods of radiofrequency application, which was the objective of this study. METHODS: Six porcine renal arteries were sectioned in their longitudinal axis and placed in the flow chamber designed to simulate physiological renal flow conditions. The catheters were placed obliquely to the artery with constant contact pressure. Radiofrequency ablations were performed using three different catheters: 4 mm/5 F solid-tip electrode, 4 mm/7 F solid-tip electrode, and open irrigated-tip 4 mm/7 F electrode. Two different powers were used (8 W and 15 W) for 30, 60 and 120 seconds. RESULTS: A total of 18 ablations were performed. More significant nerve damage was observed with the 4 mm/5 F catheter and power of 8 W only when the application duration was extended to 120 seconds. On the other hand, significant nerve damage was observed with the 4 mm/7 F catheter with all power (8 W and 15 W) and duration (30, 60, and 120 seconds) options tested. Deeper lesions were observed with the use of the irrigated catheter, regardless of power and time periods of radiofrequency application. CONCLUSIONS: The irrigated-tip catheters produce deeper lesions than solid-tip catheters and their use might be more beneficial in treating patients with renal sympathetic denervation. The clinical applicability of these results, however, should be confirmed.INTRODUÇÃO: A denervação simpática renal por meio de cateteres surgiu como estratégia adjunta para o controle da hipertensão arterial resistente. Nenhum estudo até o momento comparou os efeitos teciduais de diferentes cateteres, potências e tempos de aplicação da radiofrequência, objetivo do presente estudo. MÉTODOS: Seis artérias renais de porco foram seccionadas em seu eixo longitudinal e colocadas em uma câmara projetada para simular condições fisiológicas de fluxo renal. Os cateteres foram posicionados obliquamente à artéria, mantendo-se pressão de contato constante. Aplicações de radiofrequência foram realizadas utilizando-se três diferentes dispositivos: eletrodo de ponta sólida 4 mm/5 F, eletrodo de ponta sólida 4 mm/7 F, e eletrodo com ponta aberta irrigada 4 mm/7 F. Duas energias foram aplicadas (8 W e 15 W), durante 30 segundos, 60 segundos e 120 segundos. RESULTADOS: No total foram realizadas 18 aplicações. Injúria neural renal mais significativa foi observada utilizando-se cateter 4 mm/5 F e energia de 8 W apenas quando a duração da aplicação foi estendida a 120 segundos. Por outro lado, significante dano neural foi observado com o cateter 4 mm/7 F com todas as potências (8 W e 15 W) e durações testadas (30 segundos, 60 segundos e 120 segundos). Lesões mais profundas foram notadas quando o cateter irrigado foi utilizado, independentemente da potência e da duração da aplicação. CONCLUSÕES: O cateter com ponta irrigada produz lesões mais profundas que os cateteres de ponta sólida e seu uso pode ser mais vantajoso na denervação simpática renal. A aplicabilidade clínica desses resultados, entretanto, deve ser confirmada.Invasiva do Instituto Dante Pazzanese de CardiologiaInstituto Dante Pazzanese de CardiologiaUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUNIFESP, Depto. de PatologiaSciEL

EBV-negative lymphoepithelial-like carcinoma of the lower lip

Lymphoepithelial-like carcinoma (LEC) is a rare malignant neoplasm, which can be associated with Epstein-Barr virus (EBV) infection. Histologically, LEC is an undifferentiated carcinoma with an intermixed reactive lymphoplasmacytic infiltrate. LEC appears to be an uncommon tumor type of lip carcinoma. An 82-year-old white woman presented a lesion on her lower lip that developed over the last year. The lesion was characterized by ulceration with flat edges, hardened base, painful, and absence of regional lymphadenopathy. Microscopical analysis evidenced an intense inflammatory infiltrate, composed of lymphoplasmacytic cells, associated with scarce pleomorphic epithelial cells. Immunohistochemistry highlighted the LEC cells with strong expression of pan-CK AE1/AE3, EMA, p63, and p53. CD138 was also faintly positive. Ki-67 was >85%. In situ hybridization analysis did not show evidence of EBV. A diagnostic of EBV-negative LEC was made. We present an uncommon type of lip carcinoma, which can represent a diagnostic challenge for clinicians and pathologists

A tale of two communities: Comparing user perceptions of condominial and conventional sewer systems in Salvador, Brazil

Problems of access and quality of sanitary sewage disproportionately impact the health of populations in urban peripheries of low-and middle-income countries. The condominial sewer system is a practical, low-cost, effective, and simplified engineering approach compared to conventional sewer systems. In support of meeting the sanitation needs in highly populated urban settings, there is a need to understand the residents’ perceptions regarding the advantages and disadvantages of this sanitation model compared to conventional sewer systems. We conducted a cross-sectional study from September to December 2021 in two urban communities of Salvador, Bahia, Brazil, where condominial and conventional sewer systems had been implemented in the last five years. Of the 203 residents we interviewed, 50.7% lived in a site served by a condominial sewer system. Residents in the condominial sewer site reported not connecting to public sewage network (23.7% vs. 11.2%; p = 0.022) more often than in the conventional site. They reported more collective action to solve urban sanitation problems (69.9% vs. 54.0%; p = 0.020), such as manhole cleaning and unclogging efforts to fix plumbing. Despite these challenges, these residents expressed that the current service quality is better than it was in the previous two years. Our results suggest that even within urban periphery communities of a large Brazilian city, disparities exist in access to and quality of sanitation services that may be linked to sewage system implementation. Implementing simplified sewer systems is important to meet the growing sanitation demands of urban areas. However, these systems should also play a role in reducing sanitation disparities and the adoption of participatory approaches to meet the needs of populations in the most disadvantaged conditions. Despite challenging conditions, there is the potential for community engagement and active participation in sanitation-related matters, which could enhance the implementation and long-term sustainability of these systems

Câncer anal e sua relação com o papilomavírus humano em pacientes portadores do vírus HIV: uma revisão integrativa da literatura / Anal cancer and its relationship with the human papillomavirus in HIV infected patients: an integrative literature review

O câncer anal é uma neoplasia que, apesar de se caracterizar como rara na população em geral, vem apresentando um aumento na incidência de casos, principalmente em populações de risco, como pacientes HIV positivos e HPV positivos. Investigar a literatura quanto a relação entre o Papiloma Vírus Humano com o desenvolvimento de câncer anal em pacientes HIV positivo. Revisão Integrativa da Literatura. Os artigos foram extraídos das bases de dados Scielo, LILACS e MEDLINE/Pubmed. Foram selecionados 15 artigos entre os anos de 2012 a 2021. Há aumento na incidência e prevalência do câncer anal em pacientes HSH que convivem com HIV. Além disso, foi demonstrado ainda que, apesar de a taxa de incidência de câncer anal ser menor em pacientes com contagem de células CD4 acima de 500/μL há risco aumentado do surgimento de neoplasia anal mesmo com valores superiores de células CD4 a 500/μL por um período superior a dois anos. Os resultados encontrados enfatizam a necessidade de identificar as populações mais expostas a infecção por HPV e ao risco de desenvolvimento de neoplasia anal, afim de promover diagnostico, tratamento precoce e melhorar a expectativa de vida dessas populações

Prostatic Artery Embolization as a Primary Treatment for Benign Prostatic Hyperplasia: Preliminary Results in Two Patients

Symptomatic benign prostatic hyperplasia (BPH) typically occurs in the sixth and seventh decades, and the most frequent obstructive urinary symptoms are hesitancy, decreased urinary stream, sensation of incomplete emptying, nocturia, frequency, and urgency. Various medications, specifically 5-α-reductase inhibitors and selective α-blockers, can decrease the severity of the symptoms secondary to BPH, but prostatectomy is still considered to be the traditional method of management. We report the preliminary results for two patients with acute urinary retention due to BPH, successfully treated by prostate artery embolization (PAE). The patients were investigated using the International Prostate Symptom Score, by digital rectal examination, urodynamic testing, prostate biopsy, transrectal ultrasound (US), and magnetic resonance imaging (MRI). Uroflowmetry and postvoid residual urine volume complemented the investigation at 30, 90, and 180 days after PAE. The procedure was performed under local anesthesia; embolization of the prostate arteries was performed with a microcatheter and 300- to 500-μm microspheres using complete stasis as the end point. One patient was subjected to bilateral PAE and the other to unilateral PAE; they urinated spontaneously after removal of the urethral catheter, 15 and 10 days after the procedure, respectively. At 6-month follow-up, US and MRI revealed a prostate reduction of 39.7% and 47.8%, respectively, for the bilateral PAE and 25.5 and 27.8%, respectively, for the patient submitted to unilateral PAE. The early results, at 6-month follow-up, for the two patients with BPH show a promising potential alternative for treatment with PAE

Construindo ambientes saudáveis em Ceilândia : uma experiência de extensão do novo campus da Universidade de Brasília na maior cidade do Distrito Federal

Ilustrações do capítulo encontram-se no Anexo I do livro Universidade para o século XXI: educação e gestão ambiental na Universidade de Brasília, p. 328.Este relato tem por objetivo apresentar os resultados do primeiro projeto de extensão iniciado na FCE, “Formação comunitária para ações de promoção da saúde e da qualidade de vida - Construindo Ambientes Saudáveis”, em 2010. O cineclube é a estratégia de aproximação dos movimentos sociais de Ceilândia com a academia por meio do debate periódico de temas problematizadores relacionados à história de luta da comunidade voltada para a promoção de ambientes saudáveis. Os debates foram gravados e transcritos como forma de registro, avaliação e publicação da experiência. A memória reflexiva sobre esse projeto é coletiva e se beneficiou de comentários críticos esboçados por vários de seus membros e pessoas das comunidades de Ceilândia. O intuito foi produzir um relato polifônico, preservando a atmosfera democrática, colegiada e diversificada que tem sido idealizada pelo projeto

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost