AGN feedback and gas mixing in the core of NGC 4636

Chandra observations of NGC 4636 show disturbances in the galaxy X-ray halo, including arm-like high surface brightness features (tentatively identified as AGN driven shocks) and a possible cavity on the west side of the galaxy core. We present Chandra and XMM spectral maps of NGC 4636 which confirm the presence of the cavity and show it to be bounded by the arm features. The maps also reveal a ~15 kpc wide plume of low temperature, high abundance gas extending 25-30 kpc to the southwest of the galaxy. The cavity appears to be embedded in this plume, and we interpret the structure as being entrained gas drawn out of the galaxy core during previous episodes of AGN activity. The end of the plume is marked by a well defined edge, with significant falls in surface brightness, temperature and abundance, indicating a boundary between galaxy and group/cluster gas. This may be evidence that as well as preventing gas cooling through direct heating, AGN outbursts can produce significant gas mixing, disturbing the temperature structure of the halo and transporting metals out from the galaxy into the surrounding intra-group medium.Comment: 4 Pages, 2 colour figures, accepted for publication in ApJ Letters. Version with high quality images at http://hea-www.harvard.edu/~ejos/files/N4636_hires.pd

Captures of Red Giant Stars by Black Holes in Elliptical Galaxies: Feedback to the Hot Gas

The highly disturbed hot gas in elliptical galaxies, as revealed in many {\em Chandra} X-ray images, implies a source of energy in the galactic nucleus. In some elliptical galaxies faint X-ray ``ghost'' cavities appear without corresponding radio lobes. It has been suggested that ghost cavities are caused by short-lived activity with a timescale of $\sim 10^3-10^4$ years, but this is difficult to understand within the popular paradigm of active galactic nuclei. We suggest an episode model for ghost cavities, invoking captures of red giant stars by the black hole located at the center of the elliptical galaxies at a typical rate of $10^{-5}$yr$^{-1}$ per galaxy. The accretion of tidally disrupted red giant stars onto the black hole powers activity in a timescale of a few years. The total energy channeled into the jet/outflow during the cooling time of the hot gas is $\sim 10^{56}$ erg, which is the typical work required to form the observed cavities. In this scenario, the faint cavities are produced by the feedback following accretion of the debris of the captured red giant stars onto the black holes. We apply the present model to several elliptical galaxies and find that it can explain the formation of the ghost cavities. This model can be tested in the future by comparisons between radio and X-ray observations.Comment: 4 pages in emulateapj5.sty. to Appear in ApJ Letter

Scaling Mass Profiles around Elliptical Galaxies Observed with Chandra and XMM-Newton

We investigated the dynamical structure of 53 elliptical galaxies, based on the {\it Chandra} archival X-ray data. In X-ray luminous galaxies, a temperature increases with radius and a gas density is systematically higher at the optical outskirts, indicating a presence of a significant amount of the group-scale hot gas. In contrast, X-ray dim galaxies show a flat or declining temperature profile against radius and the gas density is relatively lower at the optical outskirts. Thus it is found that X-ray bright and faint elliptical galaxies are clearly distinguished by the temperature and gas density profile. The mass profile is well scaled by a virial radius $r_{200}$ rather than an optical-half radius $r_e$, and is quite similar at $(0.001-0.03)r_{200}$ between X-ray luminous and dim galaxies, and smoothly connects to those of clusters of galaxies. At the inner region of $(0.001-0.01)r_{200}$ or $(0.1-1)r_e$, the mass profile well traces a stellar mass with a constant mass-to-light ratio of $M/L_{\rm B}=3-10(M_{\odot}/L_{\odot})$. $M/L_{\rm B}$ ratio of X-ray bright galaxies rises up steeply beyond $0.01r_{200}$, and thus requires a presence of massive dark matter halo. From the deprojection analysis combined with the {\it XMM-Newton} data, we found that X-ray dim galaxies, NGC 3923, NGC 720, and IC 1459, also have a high $M/L_{\rm B}$ ratio of 20--30 at 20 kpc, comparable to that of X-ray luminous galaxies. Therefore, dark matter is indicated to be common in elliptical galaxies, and their distribution almost follows the NFW profile, as well as galaxy clusters.Comment: 33 pages, 11 figures, to appear in ApJ 636 No.2, ApJ 10 January 200

HST/ACS observations of shell galaxies: inner shells, shell colours and dust

AIM:Learn more about the origin of shells and dust in early type galaxies. METHOD: V-I colours of shells and underlying galaxies are derived, using HST Advanced Camera for Surveys (ACS) data. A galaxy model is made locally in wedges and subtracted to determine shell profiles and colours. We applied Voronoi binning to our data to get smoothed colour maps of the galaxies. Comparison with N-body simulations from the literature gives more insight to the origin of the shell features. Shell positions and dust characteristics are inferred from model galaxy subtracted images. RESULT: The ACS images reveal shells well within the effective radius in some galaxies (at 1.7 kpc in the case of NGC 5982). In some cases, strong nuclear dust patches prevent detection of inner shells. Most shells have colours which are similar to the underlying galaxy. Some inner shells are redder than the galaxy. All six shell galaxies show out of dynamical equilibrium dust features, like lanes or patches, in their central regions. Our detection rate for dust in the shell ellipticals is greater than that found from HST archive data for a sample of normal early-type galaxies, at the 95% confidence level. CONCLUSIONS: The merger model describes better the shell distributions and morphologies than the interaction model. Red shell colours are most likely due to the presence of dust and/or older stellar populations. The high prevalence and out of dynamical equilibrium morphologies of the central dust features point towards external influences being responsible for visible dust features in early type shell galaxies. Inner shells are able to manifest themselves in relatively old shell systems.Comment: accepted by A&A; 36 Figures, 25 pages. A version with full resolution Figures can be found here: http://www.astro.rug.nl/~sikkema/shells.p

Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene

Plants can survive a limiting nitrogen (N) supply by developing a set of N limitation adaptive responses. However, the Arabidopsis nla (nitrogen limitation adaptation) mutant fails to produce such responses, and cannot adapt to N limitation. In this study, the nla mutant was utilized to understand further the effect of NLA on Arabidopsis adaptation to N limitation. Grown with limiting N, the nla mutant could not accumulate anthocyanins and instead produced an N limitation-induced early senescence phenotype. In contrast, when supplied with limiting N and limiting phosphorus (Pi), the nla mutants accumulated abundant anthocyanins and did not show the N limitation-induced early senescence phenotype. These results support the hypothesis that Arabidopsis has a specific pathway to control N limitation-induced anthocyanin synthesis, and the nla mutation disrupts this pathway. However, the nla mutation does not affect the Pi limitation-induced anthocyanin synthesis pathway. Therefore, Pi limitation induced the nla mutant to accumulate anthocyanins under N limitation and allowed this mutant to adapt to N limitation. Under N limitation, the nla mutant had a significantly down-regulated expression of many genes functioning in anthocyanin synthesis, and an enhanced expression of genes involved in lignin production. Correspondingly, the nla mutant grown with limiting N showed a significantly lower production of anthocyanins (particularly cyanidins) and an increase in lignin contents compared with wild-type plants. These data suggest that NLA controls Arabidopsis adaptability to N limitation by channelling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis, which is important for Arabidopsis to adapt to N limitation

Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling in obesity

Innate immune signaling via TLR4 plays critical roles in pathogenesis of metabolic disorders, but the contribution of different lipid species to metabolic disorders and inflammatory diseases is less clear. GM3 ganglioside in human serum is composed of a variety of fatty acids, including long-chain (LCFA) and very-long-chain (VLCFA). Analysis of circulating levels of human serum GM3 species from patients at different stages of insulin resistance and chronic inflammation reveals that levels of VLCFA-GM3 increase significantly in metabolic disorders, while LCFA-GM3 serum levels decrease. Specific GM3 species also correlates with disease symptoms. VLCFA-GM3 levels increase in the adipose tissue of obese mice, and this is blocked in TLR4-mutant mice. In cultured monocytes, GM3 by itself has no effect on TLR4 activation; however, VLCFA-GM3 synergistically and selectively enhances TLR4 activation by LPS/HMGB1, while LCFA-GM3 and unsaturated VLCFA-GM3 suppresses TLR4 activation. GM3 interacts with the extracellular region of TLR4/MD2 complex to modulate dimerization/oligomerization. Ligand-molecular docking analysis supports that VLCFA-GM3 and LCFA-GM3 act as agonist and antagonist of TLR4 activity, respectively, by differentially binding to the hydrophobic pocket of MD2. Our findings suggest that VLCFA-GM3 is a risk factor for TLR4-mediated disease progression

The Arabidopsis plastid-signalling mutant gun1 (genomes uncoupled1) shows altered sensitivity to sucrose and abscisic acid and alterations in early seedling development

Developing seedlings of the Arabidopsis gun1 (genomes uncoupled1) mutant, which is defective in retrograde plastid-to-nucleus signalling, show several previously unrecognized mutant phenotypes. gun1 seedlings accumulated less anthocyanin than wild-type seedlings when grown in the presence of 2% (w/v) sucrose, due to lower amounts of transcripts of early anthocyanin biosynthesis genes in gun1. Norflurazon and lincomycin, which induce retrograde signalling, further decreased the anthocyanin content of sucrose-treated seedlings, and altered the temporal pattern of anthocyanin accumulation. Lincomycin treatment altered the spatial pattern of sucrose-induced anthocyanin accumulation, suggesting that plastids provide information for the regulation of anthocyanin biosynthesis in Arabidopsis seedlings. The temporal pattern of accumulation of LHCB1 transcripts differed between wild-type and gun1 seedlings, and gun1 seedlings were more sensitive to sucrose suppression of LHCB1 transcript accumulation than wild-type seedlings. Growth and development of gun1 seedlings was more sensitive to exogenous 2% sucrose than wild-type seedlings and, in the presence of lincomycin, cotyledon expansion was enhanced in gun1 seedlings compared to the wild type. gun1 seedlings were more sensitive than wild-type seedlings to the inhibition of seedling growth and development by abscisic acid. These observations clearly implicate GUN1 and plastid signalling in the regulation of seedling development and anthocyanin biosynthesis, and indicate a complex interplay between sucrose and plastid signalling pathways

Extracellular Sulfatases, Elements of the Wnt Signaling Pathway, Positively Regulate Growth and Tumorigenicity of Human Pancreatic Cancer Cells

BACKGROUND: Heparan sulfate proteoglycans (HSPGs) are control elements in Wnt signaling, which bind extracellularly to Wnt ligands and regulate their ability to interact with signal transduction receptors on the cell surface. Sulf-1 and Sulf-2 are novel extracellular sulfatases that act on internal glucosamine-6-sulfate (6S) modifications within HSPGs and thereby modulate HSPG interactions with various signaling molecules, including Wnt ligands. Emerging evidence indicates the importance of reactivated Wnt signaling in a number of cancers, including pancreatic adenocarcinoma. PRINCIPLE FINDINGS: Both Sulf proteins were upregulated in human pancreatic adenocarcinoma tumors and were broadly expressed in human pancreatic adenocarcinoma cell lines. Expression of human extracellular sulfatases Sulf-1 and Sulf-2 enhanced Wnt signaling in a reconstituted system. Three of four pancreatic adenocarcinoma cell lines tested exhibited autocrine Wnt signaling, in that extracellular Wnt ligands were required to initiate downstream Wnt signaling. Exposure of these pancreatic adenocarcinoma cells to a catalytically inactive form of Sulf-2 or siRNA-mediated silencing of endogenous Sulf-2 inhibited both Wnt signaling and cell growth. Sulf-2 silencing in two of these lines resulted in markedly reduced tumorigenesis in immunocompromised mice. CONCLUSIONS/SIGNIFICANCE: We have identified the Sulfs as potentiators of autocrine Wnt signaling in pancreatic cancer cells and have demonstrated their contribution to the growth and tumorigenicity of these cells. Since the Sulfs are extracellular enzymes, they would be attractive targets for therapy of pancreatic cancer. Our results run counter to the prevailing view in the literature that the Sulfs are negative regulators of tumorigenesis

Risk Factors for Hepatitis C Infection Among Sexually Transmitted Disease-Infected, Inner City Obstetric Patients

Objective: To test the hypothesis that our inner city obstetric patients who have been infected with sexually transmitted diseases (STDs) will have a higher prevalence of hepatitis C virus infection than the general population and to identify specific risk factors and high-risk groups. Methods: All patients in our prenatal clinic (July 1997–April 1999) who tested positive for one or more STDs were asked to return for hepatitis C antibody testing. Medical charts of all patients who returned for hepatitis C testing were reviewed. Results: A total of 106 patients with STDs were tested for hepatitis C. Positive screening tests for anti-hepatitis C antibody were found in 6.6% (7/106) of the patients (95% CI = 2.7–13.1%). This frequency is significantly higher than the hepatitis C prevalence (1.8%) in the general United States population (p = 0.006). Multiple logistic regression analysis confirmed only older age (p = 0.016) and positive HIV status (p = 0.023) to be significant predictors of hepatitis C infection. Conclusions: Inner city STD-infected obstetric patients are at high risk for hepatitis C infection compared with the general population. Increasing age and HIV-positive status are risk factors which are significantly associated with hepatitis C infection