Summary, volume 1 Final report

Voyager project study summaries on spacecraft powered by radioisotope thermoelectric generators, planetary quarantine, redundancy, and data management and contro

Effect of postactivation potentiation on fifty-meter freestyle in national swimmers

Effect of postactivation potentiation (PAP) on fifty meters freestyle in national swimmers. 2013.- The purpose of this study was to examine the effect of PAP on 50m freestyle in national-level swimmers. Four warm-ups were compared: A traditional race-specific warm-up (RSWU), upper body PAP (UBPAP), lower body PAP (LBPAP) and combined PAP warm-up (CPAP). Eighteen (10 men, 8 women) national-level swimmers participated in this study, which included seven separate testing sessions. Participants' 3 repetition maximum (3RM) of the pull-up (PU) was established in session 1. In session 2, rest periods for muscle enhancement of the upper body were determined using a medicine-ballthrow test 4, 8 and 12 minutes post UBPAP stimulus (1 x 3RM of the PU). In session 3, swimmers performed a counter movement jump 4, 8 and 12 minutes post LBPAP stimulus (1x5 jumps to a box whilst carrying 10% of the participants' body weight). The 50m freestyle tests were performed on sessions 4 to 7, preceded by each warm-up protocol and corresponding rest periods. A repeated measures ANOVA (p<0.05) and Bonferroni post hoc test revealed that RSWU elicited faster swimming times than UBPAP (29.00 ± 2.05 vs. 29.36 ± 1.88s p=0.046). Additionally, when data were split into gender, in the male group the UBPAP elicited significantly slower times than RSWU (27.51 ± 1.06 vs. 28.01 ± 1.17s p=0.047) and CPAP (27.49 ± 1.12 vs. 28.01 ± 1.17s p=0.02). These findings suggest individualized PAP warm-up may be a valuable tool to enhance performance in sprint events, particularly in male swimmers. However, the PU may not be an appropriate PAP stimulus on its own

Designing Privacy-aware Internet of Things Applications

Internet of Things (IoT) applications typically collect and analyse personal data that can be used to derive sensitive information about individuals. However, thus far, privacy concerns have not been explicitly considered in software en- gineering processes when designing IoT applications. The advent of behaviour driven security mechanisms, failing to address privacy concerns in the design of IoT applications can have security implications. In this paper, we explore how a Privacy-by-Design (PbD) framework, formulated as a set of guidelines, can help software engineers integrate data privacy considerations into the design of IoT applications. We studied the utility of this PbD framework by studying how software engineers use it to design IoT applications. We also explore the challenges in using the set of guidelines to influence the IoT applications design process. In addition to highlighting the benefits of having a PbD framework to make privacy features explicit during the design of IoT applications, our studies also surfaced a number of challenges associated with the approach. A key find- ing of our research is that the PbD framework significantly increases both novice and expert software engineers’ ability to design privacy into IoT applications

Efficacy of early neonatal vitamin A supplementation in reducing mortality during infancy in Ghana, India and Tanzania: study protocol for a randomized controlled trial

Vitamin A supplementation of 6-59 month old children is currently recommended by the World Health Organization based on evidence that it reduces mortality. There has been considerable interest in determining the benefits of neonatal vitamin A supplementation, but the results of existing trials are conflicting. A technical consultation convened by WHO pointed to the need for larger scale studies in Asia and Africa to inform global policy on the use of neonatal vitamin A supplementation. Three trials were therefore initiated in Ghana, India and Tanzania to determine if vitamin A supplementation (50,000 IU) given to neonates once orally on the day of birth or within the next two days will reduce mortality in the period from supplementation to 6 months of age compared to placebo. The trials are individually randomized, double masked, and placebo controlled. The required sample size is 40,200 in India and 32,000 each in Ghana and Tanzania. The study participants are neonates who fulfil age eligibility, whose families are likely to stay in the study area for the next 6 months, who are able to feed orally, and whose parent(s) provide informed written consent to participate in the study. Neonates randomized to the intervention group receive 50,000 IU vitamin A and the ones randomized to the control group receive placebo at the time of enrollment. Mortality and morbidity information are collected through periodic home visits by a study worker during infancy. The primary outcome of the study is mortality from supplementation to 6 months of age. The secondary outcome of the study is mortality from supplementation to 12 months of age. The three studies will be analysed independent of each other. Subgroup analysis will be carried out to determine the effect by birth weight, sex, and timing of DTP vaccine, socioeconomic groups and maternal large-dose vitamin A supplementation. The three ongoing studies are the largest studies evaluating the efficacy of vitamin A supplementation to neonates. Policy formulation will be based on the results of efficacy of the intervention from the ongoing randomized controlled trials combined with results of previous studies

Classroom management : the succesful use of behavior modification

xvii, 444 p.; 22 cm

Computers and information system, ed. 2/ Oleary

xxix, 668 hal.: ill.; 26 c

Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP

We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector

Ex vivo culture of circulating tumour cells derived from non-small cell lung cancer.

BackgroundTumour tissue-based information is limited. Liquid biopsy can provide valuable real-time information through circulating tumour cells (CTCs). Profiling and expanding CTCs may provide avenues to study transient metastatic disease.MethodsSeventy non-small cell lung cancer (NSCLC) patients were recruited. CTCs were enriched using the spiral microfluidic chip and a RosetteSep™ using bloods from NSCLC patients. CTC cultures were carried out using the Clevers media under hypoxic conditions. CTCs were characterized using immunofluorescence and mutation-specific antibodies for samples with known mutation profiles. Exome sequencing was used to characterized CTC cultures.ResultsCTCs (>2 cells) were detected in 38/70 (54.3%) of patients ranging from 0 to 385 CTCs per 7.5 mL blood. In 4/5 patients where primary tumours harboured an EGFR exon 19 deletion, this EGFR mutation was also captured in CTCs. ALK translocation was confirmed on CTCs from a patient harbouring an ALK-rearrangement in the primary tumour. Short term CTC cultures were successfully generated in 9/70 NSCLC patients. Whole exome sequencing (WES) confirmed the presence of somatic mutations in the CTC cultures with mutational signatures consistent with NSCLC.ConclusionsWe were able to detect CTCs in >50% of NSCLC patients. NSCLC patients with >2 CTCs had a poor prognosis. The short-term CTC culture success rate was 12.9%. Further optimization of this culture methodology may provide a means by which to expand CTCs derived from NSCLC patient's bloods. CTC cultures allow for expansion of cells to a critical mass, allowing for functional characterization of CTCs with the goal of drug sensitivity testing and the creation of CTC cell lines

Avian paramyxovirus type 1 infection in houbara bustards (Chlamydotis undulata macqueenii): Clinical and pathologic findings

Clinical and pathologic findings of avian paramyxovirus type 1 (PMV-1) in 19 houbara bustards (Chlamydotis undulata macqueenii) imported from Pakistan into the United Arab Emirates and one captive-bred bird are reported. Clinical signs included circling, walking backward, ataxia, opisthotonos, torticollis, recumbency, head tilt, head shaking, head tremor, tucking of head under keel, nasal discharge, conjunctivitis, and diarrhea. The length of time imported birds exhibited clinical signs varied from 4 days to 18 mo after importation. Hemagglutinating antibodies against PMV-1 were detected in the sera of all 17 birds from which blood samples were collected, and PMV-1 was isolated from pooled brain, spleen, and lung tissues from two birds with acute clinical signs. There were no distinctive gross lesions at necropsy, and histologic findings were consistent with but not pathognomonic for PMV-1. All houbara bustards managed in a captive breeding and restoration program established by the National Avian Research Center have been vaccinated against PMV-1 since October 1992, and no case of PMV-1 has been reported in this collection since that time