AMBER/VLTI high spectral resolution observations of the Brγ\gamma emitting region in HD 98922. A compact disc wind launched from the inner disc region

We analyse the main physical parameters and the circumstellar environment of the young Herbig Be star HD 98922. We present AMBER/VLTI high spectral resolution (R =12000) interferometric observations across the Br$\gamma$ line, accompanied by UVES high-resolution spectroscopy and SINFONI-AO assisted near-infrared integral field spectroscopic data. To interpret our observations, we develop a magneto-centrifugally driven disc-wind model. Our analysis of the UVES spectrum shows that HD 98922 is a young (~5x10^5 yr) Herbig Be star (SpT=B9V), located at a distance of 440(+60-50) pc, with a mass accretion rate of ~9+/-3x10^(-7) M_sun yr^(-1). SINFONI K-band AO-assisted imaging shows a spatially resolved circumstellar disc-like region (~140 AU in diameter) with asymmetric brightness distribution. Our AMBER/VLTI UT observations indicate that the Br$\gamma$ emitting region (radius ~0.31+/-0.04 AU) is smaller than the continuum emitting region (inner dust radius ~0.7+/-0.2 AU), showing significant non-zero V-shaped differential phases (i.e. non S-shaped, as expected for a rotating disc). The value of the continuum-corrected pure Br$\gamma$ line visibility at the longest baseline (89 m) is ~0.8+/-0.1, i.e. the Br$\gamma$ emitting region is partially resolved. Our modelling suggests that the observed Br$\gamma$ line-emitting region mainly originates from a disc wind with a half opening angle of 30deg, and with a mass-loss rate of ~2x10(-7) M_sun yr^(-1). The observed V-shaped differential phases are reliably reproduced by combining a simple asymmetric continuum disc model with our Br$\gamma$ disc-wind model. The Br$\gamma$ emission of HD 98922 can be modelled with a disc wind that is able to approximately reproduce all interferometric observations if we assume that the intensity distribution of the dust continuum disc is asymmetric.Comment: Accepted for publication on Astronomy \& Astrophysics. High resolution figures published on the main journal (see Astronomy & Astrophysics: Forthcoming) or at www.researchgate.net/profile/Alessio_Caratti_o_Garatti/publication

The encapsulation of DNA molecules within biomimetic lipid nanocapsules

Most of DNA synthetic complexes result from the self-assembly of DNA molecules with cationic lipids or polymers in an aqueous controlled medium. However, injection of such self-assembled complexes in medium like blood that differ from that of their formulation leads to strong instability. Therefore, DNA vectors that have physico-chemical properties and structural organisation that will not be sensitive to a completely different medium in terms of ionic and protein composition are actively sought. To this end, the goal here was to discover and optimize a nanostructured system where DNA molecules would be encapsulated in nanocapsules consisting in an oily core and a shell covered by PEG stretches obtained through a nanoemulsion process in the absence of organic solvent. This encapsulation form of DNA molecules would prevent interactions with external hostile biological fluid. The results show the entrapment of lipoplexes into lipid nanocapsules, leading to the formation of neutral 110 nm-DNA nanocapsules. They were weakly removed by the immune system, displaying an increased blood half-life, and improved carcinoma cell transfection, in comparison to the parent lipoplexes. Our results demonstrate that the fabrication of nanocapsules encapsulating hydrophilic DNA in an oily core that meet criteria for blood injection is possible

LITpro: a model fitting software for optical interferometry

9 pagesInternational audienceLITpro is a software for fitting models on data obtained from various stellar optical interferometers, like the VLTI. As a baseline, for modeling the object, it provides a set of elementary geometrical and center-to-limb darkening functions, all combinable together. But it is also designed to make very easy the implementation of more specific models with their own parameters, to be able to use models closer to astrophysical considerations. So LITpro only requires the modeling functions to compute the Fourier transform of the object at given spatial frequencies, and wavelengths and time if needed. From this, LITpro computes all the necessary quantities as needed (e.g. visibilities, spectral energy distribution, partial derivatives of the model, map of the object model). The fitting engine, especially designed for this kind of optimization, is based on a modified Levenberg-Marquardt algorithm and has been successfully tested on real data in a prototype version. It includes a Trust Region Method, minimizing a heterogeneous non-linear and non-convex criterion and allows the user to set boundaries on free parameters. From a robust local minimization algorithm and a starting points strategy, a global optimization solution is effectively achieved. Tools have been developped to help users to find the global minimum. LITpro is also designed for performing fitting on heterogeneous data. It will be shown, on an example, how it fits simultaneously interferometric data and spectral energy distribution, with some benefits on the reliability of the solution and a better estimation of errors and correlations on the parameters. That is indeed necessary since present interferometric data are generally multi-wavelengths

DETC Induces Leishmania Parasite Killing in Human In Vitro and Murine In Vivo Models: A Promising Therapeutic Alternative in Leishmaniasis

Background: Chemotherapy remains the primary tool for treatment and control of human leishmaniasis. However, currently available drugs present serious problems regarding side-effects, variable efficacy, and cost. Affordable and less toxic drugs are urgently needed for leishmaniasis. Methodology/Principal Findings: We demonstrate, by microscopy and viability assays, that superoxide dismutase inhibitor diethyldithiocarbamate (DETC) dose-dependently induces parasite killing (p,0.001) and is able to ??????sterilize?????? Leishmania amazonensis infection at 2 mM in human macrophages in vitro. We also show that DETC-induced superoxide production (p,0.001) and parasite destruction (p,0.05) were reverted by the addition of the antioxidant N-acetylcysteine, indicating that DETC-induced killing occurs through oxidative damage. Furthermore, ultrastructural analysis by electron microscopy demonstrates a rapid and highly selective destruction of amastigotes in the phagosome upon DETC treatment, without any apparent damage to the host cell, including its mitochondria. In addition, DETC significantly induced parasite killing in Leishmania promastigotes in axenic culture. In murine macrophages infected with Leishmania braziliensis, DETC significantly induced in vitro superoxide production (p = 0.0049) and parasite killing (p = 0.0043). In vivo treatment with DETC in BALB/C mice infected with Leishmania braziliensis caused a significant decrease in lesion size (p,0.0001), paralleled by a 100-fold decrease (p = 0.0087) in parasite burden. Conclusions/Significance: Due to its strong leishmanicidal effect in human macrophages in vitro, its in vivo effectiveness in a murine model, and its previously demonstrated in vivo safety profile in HIV treatment, DETC treatment might be considered as a valuable therapeutic option in human leishmaniasis, including HIV/Leishmania co-infection

Digestive and appendicular soft-parts, with behavioural implications, in a large Ordovician trilobite from the Fezouata Lagerstätte, Morocco

Trilobites were one of the most successful groups of marine arthropods during the Palaeozoic era, yet their soft-part anatomy is only known from a few exceptionally-preserved specimens found in a handful of localities from the Cambrian to the Devonian. This is because, even if the sclerotized appendages were not destroyed during early taphonomic stages, they are often overprinted by the three-dimensional, mineralised exoskeleton. Inferences about the ventral anatomy and behavioural activities of trilobites can also be derived from the ichnological record, which suggests that most Cruziana and Rusophycus trace fossils were possibly produced by the actions of trilobites. Three specimens of the asaphid trilobite Megistaspis (Ekeraspis) hammondi, have been discovered in the Lower Ordovician Fezouata Konservat-Lagerstätte of southern Morocco, preserving appendages and digestive tract. The digestive structures include a crop with digestive caeca, while the appendages display exopodal setae and slight heteropody (cephalic endopods larger and more spinose than thoracic and pygidial ones). The combination of these digestive structures and the heteropody has never been described together among trilobites, and the latter could assist in the understanding of the production of certain comb-like traces of the Cruziana rugosa group, which are extraordinarily abundant on the shallow marine shelves around Gondwana.This work has been supported by the Spanish Ministry of Economy and Competitiveness, project number CGL2012- 39471/BTE.Peer reviewe

Oxygen as a Driver of Early Arthropod Micro-Benthos Evolution

BACKGROUND: We examine the physiological and lifestyle adaptations which facilitated the emergence of ostracods as the numerically dominant Phanerozoic bivalve arthropod micro-benthos. METHODOLOGY/PRINCIPAL FINDINGS: The PO(2) of modern normoxic seawater is 21 kPa (air-equilibrated water), a level that would cause cellular damage if found in the tissues of ostracods and much other marine fauna. The PO(2) of most aquatic breathers at the cellular level is much lower, between 1 and 3 kPa. Ostracods avoid oxygen toxicity by migrating to waters which are hypoxic, or by developing metabolisms which generate high consumption of O(2). Interrogation of the Cambrian record of bivalve arthropod micro-benthos suggests a strong control on ecosystem evolution exerted by changing seawater O(2) levels. The PO(2) of air-equilibrated Cambrian-seawater is predicted to have varied between 10 and 30 kPa. Three groups of marine shelf-dwelling bivalve arthropods adopted different responses to Cambrian seawater O(2). Bradoriida evolved cardiovascular systems that favoured colonization of oxygenated marine waters. Their biodiversity declined during intervals associated with black shale deposition and marine shelf anoxia and their diversity may also have been curtailed by elevated late Cambrian (Furongian) oxygen-levels that increased the PO(2) gradient between seawater and bradoriid tissues. Phosphatocopida responded to Cambrian anoxia differently, reaching their peak during widespread seabed dysoxia of the SPICE event. They lacked a cardiovascular system and appear to have been adapted to seawater hypoxia. As latest Cambrian marine shelf waters became well oxygenated, phosphatocopids went extinct. Changing seawater oxygen-levels and the demise of much of the seabed bradoriid micro-benthos favoured a third group of arthropod micro-benthos, the ostracods. These animals adopted lifestyles that made them tolerant of changes in seawater O(2). Ostracods became the numerically dominant arthropod micro-benthos of the Phanerozoic. CONCLUSIONS/SIGNIFICANCE: Our work has implications from an evolutionary context for understanding how oxygen-level in marine ecosystems drives behaviour

Social and Hydrological Responses to Extreme Precipitations: An Interdisciplinary Strategy for Postflood Investigation

International audienceThis paper describes and illustrates a methodology to conduct postflood investigations based on interdisciplinary collaboration between social and physical scientists. The method, designed to explore the link between crisis behavioral response and hydrometeorological dynamics, aims at understanding the spatial and temporal capacities and constraints on human behaviors in fast-evolving hydrometeorological conditions. It builds on methods coming from both geosciences and transportations studies to complement existing post-flood field investigation methodology used by hydrometeorologists. The authors propose an interview framework, structured around a chronological guideline to allow people who experienced the flood firsthand to tell the stories of the circumstances in which their activities were affected during the flash flood. This paper applies the data collection method to the case of the 15 June 2010 flash flood event that killed 26 people in the Draguignan area (Var, France). As a first step, based on the collected narratives, an abductive approach allowed the identification of the possible factors influencing individual responses to flash floods. As a second step, behavioral responses were classified into categories of activities based on the respondents' narratives. Then, aspatial and temporal analysis of the sequences made of the categories of action to contextualize the set of coping responses with respect to local hydrometeorological conditions is proposed. During this event, the respondents mostly follow the pace of change in their local environmental conditions as the flash flood occurs, official flood anticipation being rather limited and based on a large-scale weather watch. Therefore, contextual factors appear as strongly influencing the individual's ability to cope with the event in such a situation

First AMBER/VLTI observations of hot massive stars

AMBER is the first near infrared focal instrument of the VLTI. It combines three telescopes and produces spectrally resolved interferometric measures. This paper discusses some preliminary results of the first scientific observations of AMBER with three Unit Telescopes at medium (1500) and high (12000) spectral resolution. We derive a first set of constraints on the structure of the circumstellar material around the Wolf Rayet Gamma2 Velorum and the LBV Eta Carinae

AMBER/VLTI interferometric observations of the recurrent Nova RS Ophiuchii 5.5 days after outburst

International audienceAims.We report on spectrally dispersed interferometric AMBER/VLTI observations of the recurrent nova RS Oph five days after the discovery of its outburst on 2006 Feb. 12. Methods: Using three baselines ranging from 44 to 86 m, and a spectral resolution of λ/δλ=1500, we measured the extension of the milliarcsecond-scale emission in the K band continuum and in the Brγ and He I 2.06 μm lines, allowing us to get an insight into the kinematics of the line forming regions. The continuum visibilities were interpreted by fitting simple geometric models consisting of uniform and Gaussian ellipses, ring and binary models. The visibilities and differential phases in the Brγ line were interpreted using skewed ring models aiming to perform a limited parametric reconstruction of the extension and kinematics of the line forming region. Results: The limited uv coverage does not allow discrimination between filled models (uniform or Gaussian ellipses) and rings. Binary models are discarded because the measured closure phase in the continuum is close to zero. The visibilities in the lines are at a low level compared to their nearby continuum, consistent with a more extended line forming region for He I 2.06 μm than Brγ. The ellipse models for the continuum and for the lines are highly flattened (b/a˜0.6) and share the same position angle (PA˜140°). Their typical Gaussian extensions are 3.1×1.9 mas, 4.9×2.9 mas and 6.3×3.6 mas for the continuum, Brγ and He I 2.06 μm lines, respectively. Two radial velocity fields are apparent in the Brγ line: a slow expanding ring-like structure (v_rad≤1800 km s-1), and a fast structure extended in the E-W direction (v_rad˜ 2500{-}3000 km s-1), a direction that coincides with the jet-like structure seen in the radio. These results confirm the basic fireball model, contrary to the conclusions of other interferometric observations conducted by Monnier et al. (2006a, ApJ, 647, L127). Based on observations collected at the European Southern Observatory, Paranal, Chile, within the programme 276.D-5049

Direct constraint on the distance of y2 Velorum from AMBER/VLTI observations

In this work, we present the first AMBER observations, of the Wolf-Rayet and O (WR+O) star binary system y2 Velorum. The AMBER instrument was used with the telescopes UT2, UT3, and UT4 on baselines ranging from 46m to 85m. It delivered spectrally dispersed visibilities, as well as differential and closure phases, with a resolution R = 1500 in the spectral band 1.95-2.17 micron. We interpret these data in the context of a binary system with unresolved components, neglecting in a first approximation the wind-wind collision zone flux contribution. We show that the AMBER observables result primarily from the contribution of the individual components of the WR+O binary system. We discuss several interpretations of the residuals, and speculate on the detection of an additional continuum component, originating from the free-free emission associated with the wind-wind collision zone (WWCZ), and contributing at most to the observed K-band flux at the 5% level. The expected absolute separation and position angle at the time of observations were 5.1±0.9mas and 66±15° respectively. However, we infer a separation of 3.62+0.11-0.30 mas and a position angle of 73+9-11°. Our analysis thus implies that the binary system lies at a distance of 368+38-13 pc, in agreement with recent spectrophotometric estimates, but significantly larger than the Hipparcos value of 258+41-31 pc