88 research outputs found
The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function
BACKGROUND: The WD motif (also known as the Trp-Asp or WD40 motif) is found in a multitude of eukaryotic proteins involved in a variety of cellular processes. Where studied, repeated WD motifs act as a site for protein-protein interaction, and proteins containing WD repeats (WDRs) are known to serve as platforms for the assembly of protein complexes or mediators of transient interplay among other proteins. In the model plant Arabidopsis thaliana, members of this superfamily are increasingly being recognized as key regulators of plant-specific developmental events. RESULTS: We analyzed the predicted complement of WDR proteins from Arabidopsis, and compared this to those from budding yeast, fruit fly and human to illustrate both conservation and divergence in structure and function. This analysis identified 237 potential Arabidopsis proteins containing four or more recognizable copies of the motif. These were classified into 143 distinct families, 49 of which contained more than one Arabidopsis member. Approximately 113 of these families or individual proteins showed clear homology with WDR proteins from the other eukaryotes analyzed. Where conservation was found, it often extended across all of these organisms, suggesting that many of these proteins are linked to basic cellular mechanisms. The functional characterization of conserved WDR proteins in Arabidopsis reveals that these proteins help adapt basic mechanisms for plant-specific processes. CONCLUSIONS: Our results show that most Arabidopsis WDR proteins are strongly conserved across eukaryotes, including those that have been found to play key roles in plant-specific processes, with diversity in function conferred at least in part by divergence in upstream signaling pathways, downstream regulatory targets and /or structure outside of the WDR regions
Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis
Blastocystis is the most prevalent eukaryotic microbe colonizing the human gut, infecting approximately 1 billion individuals worldwide. Although Blastocystis has been linked to intestinal disorders, its pathogenicity remains controversial because most carriers are asymptomatic. Here, the genome sequence of Blastocystis subtype (ST) 1 is presented and compared to previously published sequences for ST4 and ST7. Despite a conserved core of genes, there is unexpected diversity between these STs in terms of their genome sizes, guanine-cytosine (GC) content, intron numbers, and gene content. ST1 has 6,544 protein-coding genes, which is several hundred more than reported for ST4 and ST7. The percentage of proteins unique to each ST ranges from 6.2% to 20.5%, greatly exceeding the differences observed within parasite genera. Orthologous proteins also display extreme divergence in amino acid sequence identity between STs (i.e., 59%–61%median identity), on par with observations of the most distantly related species pairs of parasite genera. The STs also display substantial variation in gene family distributions and sizes, especially for protein kinase and protease gene families, which could reflect differences in virulence. It remains to be seen to what extent these inter-ST differences persist at the intra-ST level. A full 26% of genes in ST1 have stop codons that are created on the mRNA level by a novel polyadenylation mechanism found only in Blastocystis. Reconstructions of pathways and organellar systems revealed that ST1 has a relatively complete membrane-trafficking system and a near-complete meiotic toolkit, possibly indicating a sexual cycle. Unlike some intestinal protistan parasites, Blastocystis ST1 has near-complete de novo pyrimidine, purine, and thiamine biosynthesis pathways and is unique amongst studied stramenopiles in being able to metabolize ?-glucans rather than ?-glucans. It lacks all genes encoding heme-containing cytochrome P450 proteins. Predictions of the mitochondrion-related organelle (MRO) proteome reveal an expanded repertoire of functions, including lipid, cofactor, and vitamin biosynthesis, as well as proteins that may be involved in regulating mitochondrial morphology and MRO/endoplasmic reticulum (ER) interactions. In sharp contrast, genes for peroxisome-associated functions are absent, suggesting Blastocystis STs lack this organelle. Overall, this study provides an important window into the biology of Blastocystis, showcasing significant differences between STs that can guide future experimental investigations into differences in their virulence and clarifying the roles of these organisms in gut health and disease
Effects of H-2 genes on mutagen-induced micronuclei and chromosome aberrations in mouse bone-marrow cells
PIL1 participates in a negative feedback loop that regulates its own gene expression in response to shade
Cryptochromes Interact Directly with PIFs to Control Plant Growth in Limiting Blue Light
© 2016 Elsevier Inc. Sun-loving plants have the ability to detect and avoid shading through sensing of both blue and red light wavelengths. Higher plant cryptochromes (CRYs) control how plants modulate growth in response to changes in blue light. For growth under a canopy, where blue light is diminished, CRY1 and CRY2 perceive this change and respond by directly contacting two bHLH transcription factors, PIF4 and PIF5. These factors are also known to be controlled by phytochromes, the red/far-red photoreceptors; however, transcriptome analyses indicate that the gene regulatory programs induced by the different light wavelengths are distinct. Our results indicate that CRYs signal by modulating PIF activity genome wide and that these factors integrate binding of different plant photoreceptors to facilitate growth changes under different light conditions
Cryptochromes Interact Directly with PIFs to Control Plant Growth in Limiting Blue Light
© 2016 Elsevier Inc. Sun-loving plants have the ability to detect and avoid shading through sensing of both blue and red light wavelengths. Higher plant cryptochromes (CRYs) control how plants modulate growth in response to changes in blue light. For growth under a canopy, where blue light is diminished, CRY1 and CRY2 perceive this change and respond by directly contacting two bHLH transcription factors, PIF4 and PIF5. These factors are also known to be controlled by phytochromes, the red/far-red photoreceptors; however, transcriptome analyses indicate that the gene regulatory programs induced by the different light wavelengths are distinct. Our results indicate that CRYs signal by modulating PIF activity genome wide and that these factors integrate binding of different plant photoreceptors to facilitate growth changes under different light conditions
Developmental Dysplasia of the Hip before and after Increasing Community Awareness of the Harmful Effects of Swaddling
Supplementary Material for: The Prevalence of and Factors Related to Vascular Hyperintensity on T1-Weighted Imaging in Acute Ischemic Stroke
<p><b><i>Background:</i></b> Thrombus visualization in patients with
acute ischemic stroke has been detected and reported using various
imaging modalities. T1-weighted imaging (T1-WI) can depict thrombi as
hyperintense signals within vessels. Moreover, in addition to thrombi,
T1-WI hyperintensities in arteries may suggest arterial dissection.
However, the frequency of and factors related to the T1-hyperintense
vessel sign (T1-HVS) are not fully known. The aim of this study was to
clarify the prevalence of and related factors for the T1-HVS in patients
with acute ischemic stroke. <b><i>Methods:</i></b> From September 2014
through December 2015, consecutive acute ischemic stroke patients who
were admitted to our stroke unit within 7 days from symptom onset were
retrospectively recruited from the prospective registry. A T1-HVS was
defined as the presence of a hyperintense signal, with intensity higher
than surrounding brain, within the vessel lumen. Moreover, T1-HVSs were
separated into filled T1-HVSs (hyperintensity fills whole vessel lumen)
and non-filled T1-HVSs. The frequency of the T1-HVS and the timing of
emersion and the relationship between the presence of the T1-HVS and
arterial occlusion were assessed. <b><i>Results:</i></b> A total of 399
patients (139 women; median age 73 years; National Institutes of Health
Stroke Scale score 3) were enrolled in the present study. Of these, 327
(82%) patients had T1-WI on admission. Two hundred and sixty-seven (67%)
subjects had at least one follow-up T1-WI (median 6 days after
admission), and 134 (34%) cases had ≥2 follow-up T1-WI examinations. The
T1-HVS was observed in 18 patients during admission; therefore, the
frequency of the T1-HVS in acute ischemic stroke patients was 4.5% (95%
CI 2.5-6.5%). All but one (94%) of the T1-HVSs were first observed on
follow-up imaging, and the median number of days from onset to T1-HVS
appearance was 9. For patients having initial major artery occlusion and
follow-up MRI (<i>n</i> = 95), sensitivity and specificity of the
T1-HVS for persistent arterial occlusion on follow-up MR angiography
were 22 and 100%, respectively. T1-HVS persisted for a few months and
then normalized. Although there were no significant differences between
filled and non-filled T1-HVS, more patients with non-filled T1-HVS had
arterial dissection (43%) than those with filled T1-HVS (9%, <i>p</i> = 0.245). <b><i>Conclusion:</i></b>
The T1-HVS was observed in 4.5% of acute ischemic stroke patients.
T1-HVSs appeared in the subacute phase in arteries with persistent
occlusion and remained for a few months.</p
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