Swift Fox, Vulpes velox, Den Located Next to a Railroad Track in Northwestern Texas

Swift Fox (Vulpes velox) dens are typically found in areas were the vegetation is sparse, in loam soils, and with unobstructed views of the surrounding area. In 2002 a Swift Fox in northwest Texas was found in a unique den situated at the base of a hill with the entrance within 1 m of an active railroad track. Use of a den in such proximity to railroad tracks has never been previously reported

Comparisons and Trends in White-tailed Deer, Odocoileus virginianus, Body Fat in Northeastern Minnesota, 1974-1990

The relationships among locations of body fats have not been thoroughly examined in White-tailed Deer (Odocoileus virginianus). We measured bone marrow fat (n = 2995), back fat (n = 1018), kidney fat (n = 2076), and xiphoid fat (n = 1246) levels of White-tailed Deer kills from Cook and Lake counties in northeastern Minnesota during 1974-1990. For each dead deer we determined age, sex, date, and causes of mortality. All of the fat measures were correlated to varying degrees. Generally all fat measurements peaked in late autumn and subsequently began declining and reached their lowest levels in May. Fat content was negatively correlated with winter severity. Causes of mortality included predation, poaching, accidental, unknown, and auto-collisions. Predated animals had lower bone marrow (-7.42 ± 3.92) and 0.165 ± 2.30 times lower back fat and had higher amounts of kidney fat than those killed by vehicles (0.86 ± 0.43)

Swift Fox, Vulpes velox, Den Use Patterns in Northwestern Texas

Predator avoidance may be a reason why Swift Foxes (Vulpes velox) are one of the most burrow-dependent canids in North America. Typically Swift Foxes have multiple dens, which they frequently move among. As part of a larger study to reduce Coyote (Canis latrans) related mortalities on Swift Foxes, we installed artificial escape dens in areas occupied by Swift Foxes on Rita Blanca National Grassland, Dallam County, Texas. For this paper, our objective was to determine the effects of artificial escape dens on Swift Fox den use patterns. From January 2002 to August 2004 we captured, radio-collared, and monitored 55 Swift Foxes. We documented annual number of dens used, rate of den use (fidelity), distance between dens, den area, and den sharing. We compared treated (artificial dens installed) and untreated (no artificial dens) areas but found no differences in annual number of dens (P = 0.64; mean = 8), rate of den use (P = 0.96; mean = 35%), mean distance between dens (P = 0.99; mean = 2,311 m), den area (P = 0.55; mean = 5.72 km2), or den sharing (P = 0.46; mean = 42% of time). We did not observe an effect of artificial escape dens on Swift Fox den use patterns probably because artificial escape dens were designed for temporary escape cover rather than diurnal den use

Articles Occupancy Modeling of Woodpeckers: Maximizing Detections for Multiple Species with 3 Multiple Spatial Scales 4 5

Abstract 10 Numerous forest birds benefit from woodpecker presence or have similar habitat requirements. 11 Monitoring populations of forest woodpeckers can be useful for management decisions regarding 12 these and other forest species. Usefulness of monitoring efforts depends on methods employed 13 and the quality of resulting parameter estimates. Estimating the proportion of area occupied by a 14 species can be an attractive and affordable alternative to abundance or survival estimates. The 15 purpose of this study was to assess the distribution and area of occupancy for pileated 16 woodpeckers (Drycopus pileatus) and American three-toed woodpeckers (Picoides dorsalis) in 17 north-central Idaho, and to compare occupancy estimates using silent point counts, playback 18 surveys, and playback surveys that incorporated estimates of detection probability (p). We used 19 a hierarchical multi-scale framework that allowed estimation of occupancy at two spatial scales 20 and applied a removal design such that repeat visits to sampling stations was not necessary to 21 estimate p. The initial naïve estimate of occupancy (using presence-absence data) for pileate

Impact of a referral management “gateway” on the quality of referral letters; a retrospective time series cross sectional review

Background Referral management centres (RMC) for elective referrals are designed to facilitate the primary to secondary care referral path, by improving quality of referrals and easing pressures on finite secondary care services, without inadvertently compromising patient care. This study aimed to evaluate whether the introduction of a RMC which includes triage and feedback improved the quality of elective outpatient referral letters. Methods Retrospective, time-series, cross-sectional review involving 47 general practices in one primary care trust (PCT) in South-East England. Comparison of a random sample of referral letters at baseline (n = 301) and after seven months of referral management (n = 280). Letters were assessed for inclusion of four core pieces of information which are used locally to monitor referral quality (blood pressure, body mass index, past medical history, medication history) and against research-based quality criteria for referral letters (provision of clinical information and clarity of reason for referral). Results Following introduction of the RMC, the proportion of letters containing each of the core items increased compared to baseline. Statistically significant increases in the recording of ‘past medical history’ (from 71% to 84%, p < 0.001) and ‘medication history’ (78% to 87%, p = 0.006) were observed. Forty four percent of letters met the research-based quality criteria at baseline but there was no significant change in quality of referral letters judged on these criteria across the two time periods. Conclusion Introduction of RMC has improved the inclusion of past medical history and medication history in referral letters, but not other measures of quality. In approximately half of letters there remains room for further improvement

Detecting forest response to droughts with global observations of vegetation water content

Droughts in a warming climate have become more common and more extreme, making understanding forest responses to water stress increasingly pressing. Analysis of water stress in trees has long focused on water potential in xylem and leaves, which influences stomatal closure and water flow through the soil-plant-atmosphere continuum. At the same time, changes of vegetation water content (VWC) are linked to a range of tree responses, including fluxes of water and carbon, mortality, flammability, and more. Unlike water potential, which requires demanding in situ measurements, VWC can be retrieved from remote sensing measurements, particularly at microwave frequencies using radar and radiometry. Here, we highlight key frontiers through which VWC has the potential to significantly increase our understanding of forest responses to water stress. To validate remote sensing observations of VWC at landscape scale and to better relate them to data assimilation model parameters, we introduce an ecosystem-scale analog of the pressure-volume curve, the non-linear relationship between average leaf or branch water potential and water content commonly used in plant hydraulics. The sources of variability in these ecosystem-scale pressure-volume curves and their relationship to forest response to water stress are discussed. We further show to what extent diel, seasonal, and decadal dynamics of VWC reflect variations in different processes relating the tree response to water stress. VWC can also be used for inferring belowground conditions-which are difficult to impossible to observe directly. Lastly, we discuss how a dedicated geostationary spaceborne observational system for VWC, when combined with existing datasets, can capture diel and seasonal water dynamics to advance the science and applications of global forest vulnerability to future droughts

ECOSTRESS: NASA's next generation mission to measure evapotranspiration from the International Space Station

The ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station ECOSTRESS) was launched to the International Space Station on June 29, 2018. The primary science focus of ECOSTRESS is centered on evapotranspiration (ET), which is produced as level‐3 (L3) latent heat flux (LE) data products. These data are generated from the level‐2 land surface temperature and emissivity product (L2_LSTE), in conjunction with ancillary surface and atmospheric data. Here, we provide the first validation (Stage 1, preliminary) of the global ECOSTRESS clear‐sky ET product (L3_ET_PT‐JPL, version 6.0) against LE measurements at 82 eddy covariance sites around the world. Overall, the ECOSTRESS ET product performs well against the site measurements (clear‐sky instantaneous/time of overpass: r2 = 0.88; overall bias = 8%; normalized RMSE = 6%). ET uncertainty was generally consistent across climate zones, biome types, and times of day (ECOSTRESS samples the diurnal cycle), though temperate sites are over‐represented. The 70 m high spatial resolution of ECOSTRESS improved correlations by 85%, and RMSE by 62%, relative to 1 km pixels. This paper serves as a reference for the ECOSTRESS L3 ET accuracy and Stage 1 validation status for subsequent science that follows using these data

Fc-Optimized Anti-CD25 Depletes Tumor-Infiltrating Regulatory T Cells and Synergizes with PD-1 Blockade to Eradicate Established Tumors

CD25 is expressed at high levels on regulatory T (Treg) cells and was initially proposed as a target for cancer immunotherapy. However, anti-CD25 antibodies have displayed limited activity against established tumors. We demonstrated that CD25 expression is largely restricted to tumor-infiltrating Treg cells in mice and humans. While existing anti-CD25 antibodies were observed to deplete Treg cells in the periphery, upregulation of the inhibitory Fc gamma receptor (FcγR) IIb at the tumor site prevented intra-tumoral Treg cell depletion, which may underlie the lack of anti-tumor activity previously observed in pre-clinical models. Use of an anti-CD25 antibody with enhanced binding to activating FcγRs led to effective depletion of tumor-infiltrating Treg cells, increased effector to Treg cell ratios, and improved control of established tumors. Combination with anti-programmed cell death protein-1 antibodies promoted complete tumor rejection, demonstrating the relevance of CD25 as a therapeutic target and promising substrate for future combination approaches in immune-oncology

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead