6,559 research outputs found
Brown adipose tissue mitochondria: modulation by GDP and fatty acids depends on the respiratory substrates
The UCP1 [first UCP (uncoupling protein)] that is found in the mitochondria of brown adipocytes [BAT (brown adipose tissue)] regulates the heat production, a process linked to non-shivering thermogenesis. The activity of UCP1 is modulated by GDP and fatty acids. In this report, we demonstrate that respiration and heat released by BAT mitochondria vary depending on the respiratory substrate utilized and the coupling state of the mitochondria. It has already been established that, in the presence of pyruvate/malate, BAT mitochondria are coupled by faf-BSA (fatty-acid-free BSA) and GDP, leading to an increase in ATP synthesis and mitochondrial membrane potential along with simultaneous decreases in both the rates of respiration and heat production. Oleate restores the uncoupled state, inhibiting ATP synthesis and increasing the rates of both respiration and heat production. We now show that in the presence of succinate: (i) the rates of uncoupled mitochondria respiration and heat production are five times slower than in the presence of pyruvate/malate; (ii) faf-BSA and GDP accelerate heat and respiration as a result and, in coupled mitochondria, these two rates are accelerated compared with pyruvate/malate; (iii) in spite of the differences in respiration and heat production noted with the two substrates, the membrane potential and the ATP synthesized were the same; and (iv) oleate promoted a decrease in heat production and respiration in coupled mitochondria, an effect different from that observed using pyruvate/malate. These effects are not related to the production of ROS (reactive oxygen species). We suggest that succinate could stimulate a new route to heat production in BAT mitochondria
Differences in colour learning between pollen- and sucrose-rewarded bees
Rapid Communication"This is an Accepted Manuscript of an article published by Taylor & Francis in Communicative & Integrative Biology on 08 August 2015, available online: http://wwww.tandfonline.com/10.1080/19420889.2015.1052921]."Open access articleWhat bees learn during pollen collection, and how they might discriminate between flowers on the basis of the quality of this reward, is not well understood. Recently we showed that bees learn to associate colours with differences in pollen rewards. Extending these findings, we present here additional evidence to suggest that the strength and time-course of memory formation may differ between pollen- and sucrose-rewarded bees. Colour-naĆÆve honeybees, trained with pollen or sucrose rewards to discriminate coloured stimuli, were found to differ in their responses when recalling learnt information after reversal training. Such differences could affect the decision-making and foraging dynamics of individual bees when collecting different types of floral rewards
A comparative analysis of colour preferences in temperate and tropical social bees
This is the final version of the article. Available from Springer Verlag via the DOI in this record.The spontaneous occurrence of colour preferences without learning has been demonstrated in several insect species; however, the underlying mechanisms are still not understood. Here, we use a comparative approach to investigate spontaneous and learned colour preferences in foraging bees of two tropical and one temperate species. We hypothesised that tropical bees utilise different sets of plants and therefore might differ in their spontaneous colour preferences. We tested colour-naive bees and foragers from colonies that had been enclosed in large flight cages for a long time. Bees were shortly trained with triplets of neutral, UV-grey stimuli placed randomly at eight locations on a black training disk to induce foraging motivation. During unrewarded tests, the beesā responses to eight colours were video-recorded. Bees explored all colours and displayed an overall preference for colours dominated by long or short wavelengths, rather than a single colour stimulus. Naive Apis cerana and Bombus terrestris showed similar choices. Both inspected long-wavelength stimuli more than short-wavelength stimuli, whilst responses of the tropical stingless bee Tetragonula iridipennis differed, suggesting that resource partitioning could be a determinant of spontaneous colour preferences. Reward on an unsaturated yellow colour shifted the beesā preference curves as predicted, which is in line with previous findings that brief colour experience overrides the expression of spontaneous preferences. We conclude that rather than determining foraging behaviour in inflexible ways, spontaneous colour preferences vary depending on experimental settings and reflect potential biases in mechanisms of learning and decision-making in pollinating insects.We acknowledge research grant funding provided by the Royal Society for International Joint Projects and UKIERI (DST-2014-15-041). B.G.S. was funded by a PhD studentship award from MHRD, Govt. of India
Achievement goals and motivational responses in tennis: Does the context matter?
Objectives: This study examined: (a) whether athletesā goal orientations differ across training and competition; (b) whether goal orientations predict effort, enjoyment, and psychological skill use differently in training and competition; and (c) whether goal orientations predict perceived improvement in training and perceived performance in competition.
Method: Participants were 116 competitive tennis players (mean age = 19.99, SD = 5.82), who completed questionnaires measuring goal orientations, effort, enjoyment, and psychological skill use in training and competition, perceived improvement in training, and perceived performance in competition.
Results: Dependent t-tests revealed that athletes reported higher task orientation in training than in competition and higher ego orientation in competition than in training, while Pearson product-moment correlations revealed a high cross-contextual consistency for both task and ego goal orientations between training and competition. Regression analyses indicated that task orientation predicted positively effort, enjoyment, self-talk, and goal setting in both contexts, perceived improvement in training, and perceived performance in competition. An interaction effect also emerged whereby ego orientation predicted positively effort in competition only when task orientation was low or average.
Conclusions: The findings suggest that goal orientations may differ between training and competition; task orientation is the goal that should be promoted in both contexts; and the context may affect the relationship between goal orientations and effort, enjoyment, and goal setting
High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
Scanning transmission electron microscopy (STEM) provides structural analysis with sub-angstrom resolution. But the pixel-by-pixel scanning process is a limiting factor in acquiring high-speed data. Different strategies have been implemented to increase scanning speeds while at the same time minimizing beam damage via optimizing the scanning strategy. Here, we achieve the highest possible scanning speed by eliminating the image acquisition dead time induced by the beam flyback time combined with reducing the amount of scanning pixels via sparse imaging. A calibration procedure was developed to compensate for the hysteresis of the magnetic scan coils. A combination of sparse and serpentine scanning routines was tested for a crystalline thin film, gold nanoparticles, and in an in-situ liquid phase STEM experiment. Frame rates of 92, 23 and 5.8 s-1 were achieved for images of a width of 128, 256, and 512 pixels, respectively. The methods described here can be applied to single-particle tracking and analysis of radiation sensitive materials
A stochastic model for the evolution of the Web
Recently several authors have proposed stochastic models of the growth of the Web graph that give rise to power-law distributions. These models are based on the notion of preferential attachment leading to the "rich get richer" phenomenon. However, these models fail to explain several distributions arising from empirical results, due to the fact that the predicted exponent is not consistent with the data. To address this problem, we extend the evolutionary model of the Web graph by including a non-preferential component, and we view the stochastic process in terms of an urn transfer model. By making this extension, we can now explain a wider variety of empirically discovered power-law distributions provided the exponent is greater than two. These include: the distribution of incoming links, the distribution of outgoing links, the distribution of pages in a Web site and the distribution of visitors to a Web site. A by-product of our results is a formal proof of the convergence of the standard stochastic model (first proposed by Simon)
Among My Souvenirs / music by Horatio Nicholls; words by Edgar Leslie
Cover: drawing of a chest filled with letters and a Rose; Publisher: De Sylvia Brown and Henderson (New York)https://egrove.olemiss.edu/sharris_d/1086/thumbnail.jp
Modelling the EB-PVD thermal barrier coating process: Component clusters and shadow masks
Electron beam-physical vapour deposition (EB-PVD) is a commonly employed process for the production of thermal barrier coatings (TBCs), used in high performance applications such as gas turbines high-pressure aerofoil blades for the aerospace industry. Computer modelling can contribute to improved control of the coating process, important to support a continuous drive for increased efficiency. This paper considers two aspects associated with the EB-PVD coating of TBCs for commercial application: firstly, that clusters of blades are coated simultaneously in commercial coaters and, secondly, that these parts possess a complex geometry, such that shadow masks need to be taken into account. In this context, a computer model that calculates coating thickness distribution along the surface of different engine components, based on the analysis of the vapour deposition flux around complex geometries, is presented. To validate the predictive capability of the computer model two deposition trials were performed. Firstly, a cluster of components was simulated using three rotating cylinders, as a simple representation of coating multiple blades. Secondly, the effect of shadow masks was studied with an arrangement in which flat plates were welded, in the form of a U-shaped component, but with one side shorter than the other. The predicted results generated by the computer model compare favourably with those measured in the experimental runs presented. For the cluster of three cylinders, an error of 4% was obtained whilst the divergence was around 20% for the simulated shadow mask due to the fact that overall coating thickness was significantly reduced. In spite of this, the results obtained from the model were promising with respect to the degree of fit of the inverse square law. It is thought that a virtual source may be responsible for measurements being generally higher than those predicted by the model
- ā¦