Adaptation options for agricultural cultivation systems in the South Central Coast under the context of climate change: Assessment Report.

This report highlights the results of consultation meetings and field visits organized by the Department of Crop Production and the CGIAR Research Program on Climate Change, Agriculture and Food Security in Southeast Asia in association with the three offices of the Department of Agriculture and Rural Development in the South Central Coast provinces of Binh Thuan, Ninh Thuan, and Khanh Hoa, in combination with consultation with the provinces in the conference: “Summing up crops production in the Winter-Spring season in 2018-2019, implementing the Summer-Autumn season, Main rice season in 2019 for the South Central Coast and the Central Highlands” held by the Ministry of Agriculture and Rural Development in Tam Ky City, Quang Nam Province on 12 April 2019. The meetings underlined the progress made by the provinces on climate change adaptation and mitigation, options for risk reductions in agricultural production, and conversion of crop structure as results of implementing the guidelines of the provinces and the Sector, especially, solutions for reservation and efficient and economic use of water under the context of climate change. This assessment report also reviews some issues related to the agricultural transformation of the region in adapting to risks caused by climate change. They are based on comparative advantages in terms of geographical location and market of key agricultural products. This report also points out shortcomings in using land and unreasonable points in managing and using important natural resources, especially water, and provides recommendations for the agricultural transformation and inter-regional connection with the Central Highlands and the Southeast. The team also introduces climate-related risks maps and adaptation plans (CS MAP) which is applied in the five provinces in the Mekong Delta Region, and hopes this solution’s expansion shall be supported by the Ministry of Agriculture and Rural Development and the provinces

Infantile-onset Pompe disease complicated by sickle cell anemia: Case report and management considerations

Infantile-onset Pompe disease (IOPD) is a rare, severe disorder of lysosomal storage of glycogen that leads to progressive cardiac and skeletal myopathy. IOPD is a fatal disease in childhood unless treated with enzyme replacement therapy (ERT) from an early age. Sickle cell anemia (SCA) is a relatively common hemoglobinopathy caused by a specific variant in the hemoglobin beta-chain. Here we report a case of a male newborn of African ancestry diagnosed and treated for IOPD and SCA. Molecular testing confirmed tw

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke

Mapping the irrigated rice cropping patterns of the Mekong delta, Vietnam through hyper-temporal SPOT NDVI image analysis

Successful identification and mapping of different cropping patterns under cloudy conditions of a specific crop through remote sensing provides important baseline information for planning and monitoring. In Vietnam, this information is either missing or unavailable; several ongoing projects studying options with radar to avoid earth observation problems caused by the prevailing cloudy conditions have to date produced only partial successes. In this research, optical hyper-temporal Satellite Pour l'Observation de la Terre (SPOT) VEGETATION (SPOT VGT) data (1998–2008) were used to describe and map variability in irrigated rice cropping patterns of the Mekong delta. Divergence statistics were used to evaluate signature separabilities of normalized difference vegetation index (NDVI) classes generated from the iterative self-organizing data analysis technique algorithm (ISODATA) classification of 10-day SPOT NDVI image series. Based on this evaluation, a map with 77 classes was selected. Out of these 77 mapped classes, 26 classes with prior knowledge that they represent rice were selected to design the sampling scheme for fieldwork and for crop calendar characterization. Using the collected information of 112 farmers’ fields belonging to the 26 selected classes, the map produced provides highly accurate information on rice cropping patterns (94% overall accuracy, 0.93 Kappa coefficient). We found that the spatial distributions of the triple and the double rice cropping systems are highly related to the flooding regime from the Hau and Tien rivers. Areas that are highly vulnerable to flooding in the upper part and those that are saline in the north-western part of the delta mostly have a double rice cropping system, whilst areas in the central and the south-eastern parts mostly have a triple rice cropping system. In turn, the duration of flooding is highly correlated with the decision by farmers to cultivate shorter or longer duration rice varieties. The overall spatial variability mostly coincides with administrative units, indicating that crop pattern choices and water control measures are locally synchronized. Water supply risks, soil acidity and salinity constraints and the anticipated highly fluctuating rice market prices all strongly influence specific farmers’ choices of rice varieties. These choices vary considerably annually, and therefore grown rice varieties are difficult to map. Our study demonstrates the high potential of optical hyper-temporal images, taken on a daily basis, to differentiate and map a high variety of irrigated rice cropping patterns and crop calendars at a high level of accuracy in spite of cloudy condition

A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan

While the wound healing activity of curcumin (CUR) has been well-established, its clinical effectiveness remains limited due to the inherently low aqueous CUR solubility, resulting in suboptimal CUR exposure in the wound sites. Previously, we developed high-payload amorphous nanoparticle complex (or nanoplex) of CUR and chitosan (CHI) capable of CUR solubility enhancement by drug-polyelectrolyte complexation. The CUR-CHI nanoplex, however, exhibited poor colloidal stability due to its strong agglomeration tendency. Herein we hypothesized that the colloidal stability could be improved by replacing CHI with its oligomers (OCHI) owed to the better charge distribution in OCHI. The effects of key parameters in drug-polyelectrolyte complexation (i.e. pH, salt inclusion, CUR concentration, and OCHI/CUR charge ratio) on the physical characteristics and preparation efficiency of the CUR-OCHI nanoplex produced were investigated. The in vivo wound healing efficacy of the CUR-OCHI nanoplex and its cytotoxicity towards human keratinocytes cells were examined. The results showed that CUR-OCHI nanoplex exhibited prolonged colloidal stability (72 h versus 90% after 7 days versus 9 days for the native CUR resulting in smaller scars, attributed to its generation of high CUR concentration in the wound sites.Nanyang Technological UniversityAccepted versionThe authors would like to acknowledge the research funds from Vietnam Atomic Energy Institute and Nuclear Research Institute (Grant number: 07/17/VNCHN) and from Nanyang Technological University's Undergraduate Research Experience on Campus (URECA) for Suen Ern Lee

The role of State Forest Enterprises in the payments for Forest Environmental Services Programme in Vietnam

To promote pro-poor payments for environmental services, it is necessary to identify institutional options that reduce transaction costs and organisational problems associated with establishing and maintaining contracts with small-scale environmental service providers. This study examined the dual functionality of state forest enterprises (SFEs) in the implementation of the Payments for Forest Environmental Services (PFES) Program in Vietnam. We considered whether SFEs’ involvement in the programme could reduce transaction costs and organisational problems. Data were collected from Tu Ly SFE in Hoa Binh province, northern Vietnam and from implementing agencies at various institutional levels. A survey of households participating in the SFE loan programme, and two stakeholder workshops were executed in 2014. The results revealed that Tu Ly SFE plays an important role in the livelihood of many farmers. A SWOT analysis exhibited SFEs’ advantage over other state agencies in implementing national forest management programmes as there are fewer parties involved with greater autonomy and outreach in the district. This study proposes the acknowledgment of SFEs as environmental service providers in their own forestlands and to use SFEs as intermediaries in the Payments for Forest Environmental Services Programme activities

X-ray-irradiated K562 feeder cells for expansion of functional CAR-T cells

Immunotherapy, particularly CAR-T therapy has recently emerged as an innovator for cancer treatment. Gamma-irradiated K562 cells is a common and effective method to stimulated CAR-T cells prior to treatment. However, high cost and limited equipment of gamma-irradiation is drawback of this method. This requires the establishment of CAR-T-expanding alternatives, such as X-ray-irradiated K562 cells.X-ray irradiation was used to deactivate K562 cells. The post-irradiative cell survival was investigated by counting of the number of cells, staining with Trypan Blue and PI. FACS analysis was applied to detect the expression of cell surface markers. The production of CD19-CAR-T cells were executed from fresh blood donor by CD19-CAR-plasmid transfection, followed by the stimulation with X-ray-irradiated K562 feeder cells. The function of produced CAR-T cells was checked by their ability to kill Daudi cells.X-ray-irradiation inhibited the propagation and viability of K562 cells in a dose- and time-dependent manner. Interestingly, CAR-T-stimulating effectors were remained on the surface of X-ray-irradiated K562 cells. CD-19-CAR-T cells were produced successfully, suggested by number of CAR-positive cells in transfected and stimulated population, compared to un-transfected group. Lastly, our data showed that engineered CAR-T cells effectively killed Daudi cells.Our data demonstrated the efficacy of X-ray on deactivation K562 feeder cells which subsequently stimulated and expanded functional CAR-T cells. Thus, X-ray can be used as an alternative to inactivate K562 cells prior to using as a feeder of CAR-T cells

Perceptions of Present and Future Climate Change Impacts on Water Availability for Agricultural Systems in the Western Mediterranean Region

Many Mediterranean countries have experienced water shortages during the last 20 years and future climate change projections foresee further pressure on water resources. This will have significant implications for irrigation water management in agricultural systems in the future. Through qualitative and quantitative empirical research methods carried out on a case study on four Mediterranean farming systems located in Oristano, Italy, we sought to understand the relationship between farmers’ perceptions of climate change (i.e., increased temperature and decreased precipitation) and of present and future water availability for agriculture as forecasted by climatic and crop models. We also explored asymmetries between farmers’ perceptions and present and future climate change and water scenarios as well as factors influencing perceptions. Our hypotheses were that farmers’ perceptions are the main drivers of actual water management practices and that sustainable practices can emerge from learning spaces designed from the understanding of the gaps between perceptions and scientific evidences. Results showed that most farmers perceived that climate change is occurring or will occur in their area. They also perceived that there has been an increased temperature trend, but also increased precipitation. Therefore, they are convinced that they have and will have enough irrigation water for agriculture in the near future, while climate change projections foresee an increasing pressure on water resources in the Mediterranean region. Such results suggest the need for (i) irrigation management policies that take into account farmers’ perceptions in order to promote virtuous behaviors and improve irrigation water use efficiency; (ii) new, well-designed learning spaces to improve the understanding on climate change expectations in the near future in order to support effective adaptive responses at the farm and catchment scales

Twelve-Month Outcomes of the AFFINITY Trial of Fluoxetine for Functional Recovery After Acute Stroke: AFFINITY Trial Steering Committee on Behalf of the AFFINITY Trial Collaboration

Background and Purpose: The AFFINITY trial (Assessment of Fluoxetine in Stroke Recovery) reported that oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and seizures. After trial medication was ceased at 6 months, survivors were followed to 12 months post-randomization. This preplanned secondary analysis aimed to determine any sustained or delayed effects of fluoxetine at 12 months post-randomization. Methods: AFFINITY was a randomized, parallel-group, double-blind, placebo-controlled trial in adults (n=1280) with a clinical diagnosis of stroke in the previous 2 to 15 days and persisting neurological deficit who were recruited at 43 hospital stroke units in Australia (n=29), New Zealand (4), and Vietnam (10) between 2013 and 2019. Participants were randomized to oral fluoxetine 20 mg once daily (n=642) or matching placebo (n=638) for 6 months and followed until 12 months after randomization. The primary outcome was function, measured by the modified Rankin Scale, at 6 months. Secondary outcomes for these analyses included measures of the modified Rankin Scale, mood, cognition, overall health status, fatigue, health-related quality of life, and safety at 12 months. Results: Adherence to trial medication was for a mean 167 (SD 48) days and similar between randomized groups. At 12 months, the distribution of modified Rankin Scale categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio, 0.93 [95% CI, 0.76–1.14]; P =0.46). Compared with placebo, patients allocated fluoxetine had fewer recurrent ischemic strokes (14 [2.18%] versus 29 [4.55%]; P =0.02), and no longer had significantly more falls (27 [4.21%] versus 15 [2.35%]; P =0.08), bone fractures (23 [3.58%] versus 11 [1.72%]; P =0.05), or seizures (11 [1.71%] versus 8 [1.25%]; P =0.64) at 12 months. Conclusions: Fluoxetine 20 mg daily for 6 months after acute stroke had no delayed or sustained effect on functional outcome, falls, bone fractures, or seizures at 12 months poststroke. The lower rate of recurrent ischemic stroke in the fluoxetine group is most likely a chance finding. REGISTRATION: URL: http://www.anzctr.org.au/ ; Unique identifier: ACTRN12611000774921

Changing livelihood options as adaptation: A comparative analysis of three flood control schemes in the Vietnamese Mekong Delta

Population, Development, and the Environment - Challenges to Achieving the Sustainable Development Goals in the Asia Pacific137-154Singapor