From teaching physics to teaching children : beginning teachers learning from pupils

This paper discusses the development of beginning physics teachers' pedagogical content knowledge (PCK) in the context of teaching basic electricity during a one-year Professional Graduate Diploma in Education course (PGDE) and beyond. This longitudinal study used repeated semi-structured interviews over a period of four-and-a-half years. The interview schedule followed a line of development through the secondary school electrical syllabus in Scotland. Fifteen student teachers were interviewed during the PGDE year. Six of them were followed up at the end of the Induction Year (their first year as a newly qualified teacher), and again two-and-a-half years later. Thematic analysis of the interviews showed that before the beginning teachers had taught any classes, their initial focus was on how to transform their own subject matter knowledge (SMK) about electricity into forms that were accessible to pupils. As the beginning teachers gained experience working with classes, they gave vivid descriptions of interacting with particular pupils when teaching electricity which showed the development of their pedagogical knowledge. This played a significant role in the teachers' change of focus from teaching physics to teaching children as they transformed their SMK into forms that were accessible to pupils and developed their general pedagogical knowledge

Sustainability of beef production from brigalow lands after cultivation and mining. 1. Sown pasture growth and carrying capacity

Context: New Acland coal mine in south-eastern Queensland is seeking to rehabilitate mined land to pastures that are safe, stable and sustainable for beef production. Little is known of the productivity and sustainability of grazing previously mined land in the Darling Downs study region. Additionally, information is required to specify management guidelines for sustainable grazing of regional land types retired from cultivation. Aims: Identify pasture growth characteristics, rainfall use efficiencies and long-term carrying capacities of subtropical sown pastures established on lands rehabilitated after open-cut coal mining in comparison to sown pastures established on un-mined but previously cultivated lands. Methods: Pasture growth and quality (% nitrogen) were observed using the Swiftsynd methodology in ungrazed exclosures with three sites on rehabilitated lands of the Acland Grazing Trial over a 5-year period (2014–2018), and 13 sites on unmined lands over periods of 2–5 years providing data for modelling pasture growth. Key results: Peak pasture yield (TSDM for autumn harvests) averaged for 2017 and 2018 was greater (P < 0.1) on rehabilitated sites than unmined Poplar Box land type sites (5957 and 2233 kg/ha respectively) but similar to Brigalow Uplands and Mountain Coolibah land type sites (3946 and 3413 kg/ha respectively). Pasture rundown was evident, with pasture N uptake decreasing over 5 years at some sites. Soil mineral N supply (potentially mineralisable N and mineral N) in spring was a useful indicator of N uptake over the following growing season. Simulations using the GRASP pasture growth model for the grazing trial period predicted rainfall use efficiencies of 12.0, 7.0, 9.1 and 4.8 kg/ha.mm rainfall for rehabilitated sites and unmined sites on Brigalow Uplands, Mountain Coolibah and Poplar Box land types respectively. Long-term carrying capacities based on estimates of long-term median pasture growth and 30% utilisation were 4.39, 3.58 and 5.92 ha/adult equivalent respectively for the unmined land types, and 2.45 ha/adult equivalent for the rehabilitated lands. Conclusions: Rehabilitated land can be as productive as unmined but previously cultivated land. Implications: Grazing management plans for sustainable management of mined and unmined lands can be developed using data from the present study. The plans will assist with the transition of rehabilitated lands to commercial agriculture

Sustainability of beef production from brigalow lands after cultivation and mining. 3. Pasture rundown, climate and grazing pressure effects

Context: The Acland Land System overlying the Walloon sandstone coal deposits in southern Queensland is generally marginal for cropping but well suited to grazing, and thus cultivated land is commonly returned to pasture. Rehabilitation of these lands after open-cut coal mining seeks to be safe, stable and self-sustaining to satisfy requirements for ecologically sustainable development. Aims: The present paper evaluates the sustainability and economic viability of beef production on (a) lands retired from cultivation and then rehabilitated with sown pastures after open-cut coal mining at the New Acland mine site, and (b) similar nearby pasture lands that were not mined but were also retired from cultivation. Methods: The GRASP grazing systems model was modified and calibrated with short-term (5-year) grazing trial data (soil, pasture and cattle observations), and then used with long-term (60-year) weather data to estimate effects of land type, pasture rundown, climate and grazing pressure on productivity and economic returns. The productivity of three rehabilitated sites and 15 unmined sites were evaluated, including pastures on six commercial properties. Key results: Estimates of long-term mean annual growth of pastures on unmined lands retired from cultivation on three land types (Mountain Coolibah, Brigalow Uplands and Poplar Box) were 3398, 2817 and 2325 kg/ha respectively. Pasture growth was greater on rehabilitated lands; 3736 kg/ha on the site most typical of rehabilitated lands and a mean of 4959 kg/ha across three sites. Seasonal conditions had large effects on cattle liveweight gain (133–213 kg/head per year during the trial); however, pasture growth was the main driver of beef production and economic returns per hectare. In GRASP, potential nitrogen uptake was used to influence key pasture growth processes and accounted for 64% of variation in observed annual growth. The short-term lift and subsequent rundown in productivity typically associated with sown pastures was estimated to have increased mean annual pasture and cattle productivity during the 2014–2018 trial period by up to 17% and 25% respectively. Estimates of long-term mean annual beef production and economic returns for the unmined lands were less than estimated for rehabilitated lands and were 139 kg/head.year (45 kg/ha.year) and AU$154/adult equivalent. Conclusions: Rehabilitated lands were found to be sustainable for beef production at grazing pressures up to 30% utilisation of annual pasture growth, and comparable with grazing systems on native and sown pastures in good condition. Pastures on unmined lands retired from cultivation had reduced productivity. Implications: Overgrazing is a significant and on-going residual risk to sustainable production. Grazing regimes need to continually adjust for changes in novel landscapes, pasture condition and climate. The methods used in the present study could be applied more generally

The effect of bleach duration and age on the ERG photostress test

Background: The ERG photostress test assesses the recovery of the focal 41 Hz ERG following exposure to a bright light that bleaches a significant proportion of photopigment. The aims of this study were: 1) to compare the repeatability of the ERG photostress test recovery time constant following long and short duration light exposure, and 2) to determine the effect of age on the ERG photostress test recovery time constant. Methods: Focal 41 Hz ERGs were recorded from 23 participants (age range 20–71 years) at 20-second intervals for 5 minutes following either a short-duration (photoflash) or long-duration (equilibrium) light exposure. After a 5-minute wash-out period, the procedure was repeated using the second bleach modality. The time constant of cone recovery was determined by fitting an exponential model to the amplitude recovery data. The whole procedure was repeated on a second occasion. The co-efficient of repeatability (CoR) was calculated for each bleaching technique. The relationship between the time constant of recovery and age was investigated (Pearson’s correlation coefficient). Results: The time constant of recovery following an equilibrium bleach was more repeatable than recovery following a photoflash (CoR = 85s and 184s respectively). Eight trials (from seven participants) failed to show a reduction in amplitude following the photoflash, suggesting that a blink or fixation loss had occurred. All participants were reliably light-adapted by the equilibrium bleach. For the equilibrium bleach data, the time constant of recovery increased with age at a rate of 27 seconds per decade. Conclusions: The equilibrium bleach was more reliable and repeatable than the photoflash. Increasing participant age was shown to result in a lengthening of the recovery time constant, of a magnitude comparable to previously published psychophysical data

HIV infection and stroke:current perspectives and future directions

HIV infection can result in stroke via several mechanisms, including opportunistic infection, vasculopathy, cardioembolism, and coagulopathy. However, the occurrence of stroke and HIV infection might often be coincidental. HIV-associated vasculopathy describes various cerebrovascular changes, including stenosis and aneurysm formation, vasculitis, and accelerated atherosclerosis, and might be caused directly or indirectly by HIV infection, although the mechanisms are controversial. HIV and associated infections contribute to chronic inflammation. Combination antiretroviral therapies (cART) are clearly beneficial, but can be atherogenic and could increase stroke risk. cART can prolong life, increasing the size of the ageing population at risk of stroke. Stroke management and prevention should include identification and treatment of the specific cause of stroke and stroke risk factors, and judicious adjustment of the cART regimen. Epidemiological, clinical, biological, and autopsy studies of risk, the pathogenesis of HIV-associated vasculopathy (particularly of arterial endothelial damage), the long-term effects of cART, and ideal stroke treatment in patients with HIV are needed, as are antiretrovirals that are without vascular risk

LiverScreen project: study protocol for screening for liver fibrosis in the general population in European countries

Background: The development of liver cirrhosis is usually an asymptomatic process until late stages when complications occur. The potential reversibility of the disease is dependent on early diagnosis of liver fibrosis and timely targeted treatment. Recently, the use of non-invasive tools has been suggested for screening of liver fibrosis, especially in subjects with risk factors for chronic liver disease. Nevertheless, large population-based studies with cost-effectiveness analyses are still lacking to support the widespread use of such tools. The aim of this study is to investigate whether non-invasive liver stiffness measurement in the general population is useful to identify subjects with asymptomatic, advanced chronic liver disease. Methods: This study aims to include 30,000 subjects from eight European countries. Subjects from the general population aged ≥ 40 years without known liver disease will be invited to participate in the study either through phone calls/letters or through their primary care center. In the first study visit, subjects will undergo bloodwork as well as hepatic fat quantification and liver stiffness measurement (LSM) by vibration-controlled transient elastography. If LSM is ≥ 8 kPa and/or if ALT levels are ≥1.5 x upper limit of normal, subjects will be referred to hospital for further evaluation and consideration of liver biopsy. The primary outcome is the percentage of subjects with LSM ≥ 8kPa. In addition, a health economic evaluation will be performed to assess the cost-effectiveness and budget impact of such an intervention. The project is funded by the European Commission H2020 program. Discussion: This study comes at an especially important time, as the burden of chronic liver diseases is expected to increase in the coming years. There is consequently an urgent need to change our current approach, from diagnosing the disease late when the impact of interventions may be limited to diagnosing the disease earlier, when the patient is asymptomatic and free of complications, and the disease potentially reversible. Ultimately, the LiverScreen study will serve as a basis from which diagnostic pathways can be developed and adapted to the specific socio-economic and healthcare conditions in each country

Drosophila IAP1-Mediated Ubiquitylation Controls Activation of the Initiator Caspase DRONC Independent of Protein Degradation

Ubiquitylation targets proteins for proteasome-mediated degradation and plays important roles in many biological processes including apoptosis. However, non-proteolytic functions of ubiquitylation are also known. In Drosophila, the inhibitor of apoptosis protein 1 (DIAP1) is known to ubiquitylate the initiator caspase DRONC in vitro. Because DRONC protein accumulates in diap1 mutant cells that are kept alive by caspase inhibition (“undead” cells), it is thought that DIAP1-mediated ubiquitylation causes proteasomal degradation of DRONC, protecting cells from apoptosis. However, contrary to this model, we show here that DIAP1-mediated ubiquitylation does not trigger proteasomal degradation of full-length DRONC, but serves a non-proteolytic function. Our data suggest that DIAP1-mediated ubiquitylation blocks processing and activation of DRONC. Interestingly, while full-length DRONC is not subject to DIAP1-induced degradation, once it is processed and activated it has reduced protein stability. Finally, we show that DRONC protein accumulates in “undead” cells due to increased transcription of dronc in these cells. These data refine current models of caspase regulation by IAPs