Stable carbon, nitrogen and sulphur isotope analysis of permafrost preserved human hair from rescue excavations (2009, 2010) at the precontact site of Nunalleq, Alaska

Acknowledgments This work was funded by an Arts and Humanities Research Council (AH/K006029/1) grant awarded to Rick Knecht, Kate Britton and Charlotta Hillerdal (Aberdeen); an AHRC-LabEx award (AH/N504543/1) to KB, RK, Keith Dobney (Liverpool) and Isabelle Sidéra (Nanterre); the Carnegie Trust to the Universities of Scotland (travel grant to KB); and the Max Planck Institute for Evolutionary Anthropology. The onsite collection of samples was carried out by staff and students from the University of Aberdeen, volunteer excavators and the residents of Quinhagak. We had logistical and planning support for fieldwork by the Qanirtuuq Incorporated, Quinhagak, Alaska, and the people of Quinhagak, who we also thank for sampling permissions. Special thanks to Warren Jones and Qanirtuuq Incorporated (especially Michael Smith and Lynn Church), and to all Nunalleq project team members, in Aberdeen and at other institutions, particularly Charlotta Hillerdal and Edouard Masson-Maclean (Aberdeen) for comments on earlier versions of this manuscript, and also to Véronique Forbes, Ana Jorge, Carly Ameen and Ciara Mannion (Aberdeen) for their inputs. Thanks also to Michelle Alexander (York). Finally, thank you to Ian Scharlotta (Alberta) for inviting us to contribute to this special issue, to the Editor, and to three anonymous reviewers, whose suggestions and recommended changes to an earlier version of this manuscript greatly improved the paper.Peer reviewedPublisher PD

Stable carbon, nitrogen and sulphur isotope analysis of permafrost preserved human hair from rescue excavations (2009, 2010) at the precontact site of Nunalleq, Alaska

AbstractThe reconstruction of diet and subsistence strategies is integral to understanding hunter-gatherer societies in the past, and is particularly of interest in high latitude environments as they can illuminate human-environmental interactions and adaptations. Until recently, very little archaeological research had been undertaken on the Bering Sea coasts of the Yukon-Kuskokwim Delta, and relatively little is known about precontact lifeways in this region. Here, we present stable carbon, nitrogen and sulphur isotope data from non-mortuary human hair excavated from Nunalleq (c. 1300CE–1750CE) – a precontact village site in Western Alaska. Now the focus of a major research project, excavations at Nunalleq began as a rescue excavation, as the site is eroding rapidly into the Bering Sea. Following an initial pilot study on cut strands representing a small number of individuals, a larger body of isotope data has now been generated from the first phase of the investigations of Nunalleq (2009, 2010). These new data, including sulphur isotope values, provide further evidence for the subsistence strategy at the site, including a mixed diet of marine and terrestrial foods (but likely dominated by salmonids). In addition, these new data from Nunalleq highlight some dietary variability amongst the inhabitants of the site. Analyses of additional longer hair strands suggest this variability may not be exclusively due to seasonal variation, and may evidence inter-personal dietary differences. Data from Nunalleq are compared to isotope data from previous studies of Thule-era and earlier Alaskan sites, and to isotope data from Thule sites in Canada and Greenland and the potential of ongoing and future research at the site is discussed, along with the implications for our understanding of Thule subsistence strategies and precontact lifeways on the Yukon-Kuskokwim Delta

High-Resolution AMS Dating of Architecture, Boulder Artworks and the Transition to Farming at Lepenski Vir

The archaeological site of Lepenski Vir is widely known after its remarkable stone art sculptures that represent a unique and unprecedented case of Holocene hunter-gatherer creativity. These artworks were found largely associated with equally unique trapezoidal limestone building floors around their centrally located rectangular stone-lined hearths. A debate has raged since the discovery of the site about the chronological place of various discovered features. While over years different views from that of the excavator about the stratigraphy and chronology of the site have been put forward, some major disagreements about the chronological position of the features that make this site a key point of reference in European Prehistory persist. Despite challenges of re-analyzing the site's stratigraphy from the original excavation records, taphonomic problems, and issues of reservoir offsets when providing radiocarbon measurements on human and dog bones, our targeted AMS (Accelerator Mass Spectrometry) dating of various contexts from this site with the application of Bayesian statistical modelling allows us to propose with confidence a new and sound chronological framework and provide formal estimates for several key developments represented in the archaeological record of Lepenski Vir that help us in understanding the transition of last foragers to first farmers in southeast Europe as a whole

Finding Mycenaeans in Minoan Crete? Isotope and DNA analysis of human mobility in Bronze Age Crete.

We undertook a large-scale study of Neolithic and Bronze Age human mobility on Crete using biomolecular methods (isotope analysis, DNA), with a particular focus on sites dating to the Late Bronze Age ('Late Minoan') period. We measured the strontium and sulphur isotope values of animal remains from archaeological sites around the island of Crete to determine the local baseline values. We then measured the strontium and sulphur values of humans from Late Neolithic and Bronze Age sites. Our results indicate that most of the humans have sulphur and strontium isotope values consistent with being local to Crete, showing no evidence for a wide-scale movement of people from the Greek mainland or other areas away from Crete in these time periods. However, we found four individuals from the late Bronze Age (Late Minoan III) cemetery of Armenoi with sulphur isotope values not typically found in Crete and are instead consistent with an origin elsewhere. This cemetery at Armenoi also has one of only a few examples of the newly adopted Mycenaean Linear B script on Crete found outside of the palace sites, pointing to an influence (trade and possible migration) from the mainland, which may then be the place of origin of these four individuals. DNA (mtDNA) studies of eight Late Bronze Age individuals from Armenoi have results consistent with people living in Aegean region at this time and cannot be used to distinguish between individuals from Crete ('Minoans') and the Greek mainland ['Mycenaeans'])

The emergence of extramural cemeteries in Neolithic southeast Europe: a formally modeled chronology for Cernica, Romania

The emergence of separate cemeteries for disposal of the dead represents a profound shift in mortuary practice in the Late Neolithic of southeast Europe, with a new emphasis on the repeated use of a specific space distinct from, though still often close to, settlements. To help to time this shift more precisely, this paper presents 25 dates from 21 burials in the large cemetery at Cernica, in the Lower Danube valley in southern Romania, which are used to formally model the start, duration of use and end of the cemetery. A further six dates were obtained from four contexts for the nearby settlement. Careful consideration is given to the possibility of environmental and dietary offsets. The preferred model, without freshwater reservoir offsets, suggests that use of the Cernica cemetery probably began in 5355–5220 cal BC (95% probability) and ended in 5190–5080 cal BC (28% probability) or 5070–4940 (67% probability). The implications of this result are discussed, including with reference to other cemeteries of similar age in the region, the nature of social relations being projected through mortuary ritual, and the incorporation of older, Mesolithic, ways of doing things into Late Neolithic mortuary practice

Response to Bonsall et al. “Food for thought: Re-assessing mesolithic diets in the iron gates"

Here, we reply to the attempt by Bonsall et al. (2015) to re-assess our results from the sulfur isotopic study of bone collagen from Mesolithic and Neolithic sites along the Danube in the Iron Gates Gorges area (Nehlich et al. 2010). Although we are highly interested to see our data re-assessed, we found certain misinterpretations, mistaken assumptions, and factual errors regarding our results. Therefore, we want to respond and re-assess our previous data, too. We establish for a few individuals a quantitative dietary reconstruction to demonstrate the reliability of our earlier interpretations

A calf for all seasons? The potential of stable isotope analysis to investigate prehistoric husbandry practices

The Early Bronze Age barrows at Irthlingborough and Gayhurst in central England are notable for the large number of cattle (Bos taurus) remains associated with their human Beaker burials. Previous work using strontium isotope analysis has indicated that most of the cattle analysed, and one aurochs (Bos primigenius), were of local origin [Towers, J., Montgomery, J., Evans, J., Jay, M., Parker Pearson, M., 2010. An investigation of the origins of cattle and aurochs deposited in the Early Bronze Age barrows at Gayhurst and Irthlingborough. Journal of Archaeological Science 37,508-515.]. In this study, stable isotope analysis of enamel and bone was carried out to investigate whether the mature cattle had experienced similar husbandry practices, climate and environment. Bulk carbon, nitrogen and sulphur isotope analysis of collagen suggested most were consuming similar sources of plant protein from environments probably local to the sites and this was supported by high resolution intra-enamel carbon isotope profiles. Oxygen isotope profiles indicated the aurochs and most of the cattle experienced similar climatic regimes: the only exception being an animal with a non-local strontium isotope ratio. However, a comparison of seasonality profiles of the local animals using estimated tooth formation times showed that there was no consistency in season of birth: the animals appeared to have been born throughout the year. Cattle can breed throughout the year but it requires considerable human effort and intervention to successfully overwinter young stock; it is therefore unlikely to have been carried out without good reason and benefit if winters were harsh. One reason is to ensure a continuous supply of milk. Measuring oxygen isotope profiles to identify year-round calving may thus be a potential indicator of dairying economies

Carbon and nitrogen isotopic ratios in archaeological and modern Swiss fish as possible markers for diachronic anthropogenic activity in freshwater ecosystems

The aimof this study is to investigate isotopic variability in archaeological (n=85) and modern (n=29) freshwater fish specimens from Switzerland. Here, carbon (δ13C) and nitrogen (δ15N) stable isotope ratio analysiswas performed on bone collagen samples of pike (Esox lucius), perch (Perca fluviatilis), barbel (Barbus barbus), roach (Rutilus rutilus) and carp (Cyprinus carpio) fromeleven archaeological (11th to 18/19th centuries CE) andmodern sites. The archaeological vs. modern fish data revealed significant isotopic differences for pike, perch and barbel (δ13Cp≤ 0.03; δ15Np≤ 0.008), and provides possible evidence for a temporal change in Swiss aquatic ecosystems from Medieval to modern times. In comparison to archaeological fish (δ13C mean ± SD; −23.3 ± 1.6‰; δ15N mean ± SD; 8.3 ± 1.8‰), the modern fish samples show decreased δ13C and increased δ15N values (δ13C mean± SD; −27.4 ± 2.3‰; δ15N mean± SD; 12.5± 4.1‰) that can be associated with anthropogenic effects: fossil fuel combustion, deforestation and organic waste in the form of sewage and fertilizers. The isotopic signatures of archaeological fish remains indicate a local fishery practice, but also the exploitation of distant fishing grounds and freshwater fish transportation. Furthermore, a diachronic isotopic trend is observed in young perch from sites in Basel, dating between the 12th and 15/16th centuries CE, and the isotopic data from the Rhine freshwater fish (18/19th century CE) suggests that a significant shift in the river's trophic state was possibly caused by organic pollution fromurban and industrial wastewater. This retrospective research illustrates possible natural processes and human activitieswhich can cause differences in fish stable isotope data and highlights the ability to elucidate changes in past bodies of water. Furthermore, this study provides an interpretative framework for additional palaeoenvironmental studies and modern restoration projects focused on freshwater ecosystems

Role of adrenoreceptors in manifestation of the bonding activity of pyloric sphincter and small intestine

The radiolysis process of a two-phase heat carrier in the reactor core was studied. A mathematical model of two-phase heat carrier radiolysis, techniques of calculating the model parameters are developed. A program complex is developed realizing the model of radiolysis, as well as the techniques of calculating the model parameters; simulation of radiolysis in AST of integral construction revealed the availability of fields of growth and decrease of radiolysis products concentration and a curve of steady states. Prediction of corrosion behaviour of fuel elements and technological chennels is used at RBMK. Prediction of corrosion behaviour of fuel element shells and technological channels increases APP reliability and safety. The field of application covers designing a reactor and development of water-chemical modesAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Carbon and nitrogen isotopic ratios in archaeological and modern Swiss fish as possible markers for diachronic anthropogenic activity in freshwater ecosystems

The aimof this study is to investigate isotopic variability in archaeological (n=85) and modern (n=29) freshwater fish specimens from Switzerland. Here, carbon (δ13C) and nitrogen (δ15N) stable isotope ratio analysiswas performed on bone collagen samples of pike (Esox lucius), perch (Perca fluviatilis), barbel (Barbus barbus), roach (Rutilus rutilus) and carp (Cyprinus carpio) fromeleven archaeological (11th to 18/19th centuries CE) andmodern sites. The archaeological vs. modern fish data revealed significant isotopic differences for pike, perch and barbel (δ13Cp≤ 0.03; δ15Np≤ 0.008), and provides possible evidence for a temporal change in Swiss aquatic ecosystems from Medieval to modern times. In comparison to archaeological fish (δ13C mean ± SD; −23.3 ± 1.6‰; δ15N mean ± SD; 8.3 ± 1.8‰), the modern fish samples show decreased δ13C and increased δ15N values (δ13C mean± SD; −27.4 ± 2.3‰; δ15N mean± SD; 12.5± 4.1‰) that can be associated with anthropogenic effects: fossil fuel combustion, deforestation and organic waste in the form of sewage and fertilizers. The isotopic signatures of archaeological fish remains indicate a local fishery practice, but also the exploitation of distant fishing grounds and freshwater fish transportation. Furthermore, a diachronic isotopic trend is observed in young perch from sites in Basel, dating between the 12th and 15/16th centuries CE, and the isotopic data from the Rhine freshwater fish (18/19th century CE) suggests that a significant shift in the river's trophic state was possibly caused by organic pollution fromurban and industrial wastewater. This retrospective research illustrates possible natural processes and human activitieswhich can cause differences in fish stable isotope data and highlights the ability to elucidate changes in past bodies of water. Furthermore, this study provides an interpretative framework for additional palaeoenvironmental studies and modern restoration projects focused on freshwater ecosystems.status: publishe