Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa in healthy female rats

Background. Earlier in an experiment on rats, we showed that newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa has a significant modulating effect on the parameters of metabolism and the autonomic nervous and endocrine systems. In this study, conducted in line with the concepts of neuroendocrine-immune complex and functional-metabolic continuum, data on the immunomodulatory effects of these waters on the same rats. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 230-290 g divided into 4 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Rats of the second (control) group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. The rats of the main groups received the water "Myroslava" and "Khrystyna". The object of the study were the immune parameters of the thymus, spleen and blood. Results. The method of discriminant analysis revealed 12 parameters, according to which the animals loaded with mineral waters differed significantly from both control and intact animals. Classification accuracy is 86%. However, the difference between the immunotropic effects of mineral waters of different mineralization concerns only 9 parameters. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have both similar and specific immunomodulating effects on healthy old female rats with weekly use. This provides a basis for preclinical studies

Transcriptional Network Involved in Drought Response and Adaptation in Cereals

Drought is the major abiotic stress in many wheat environments, decreasing grain yields and farmer’s income. Finding ways to improve drought tolerance in wheat is therefore a global effort. Transcription factors (TFs) play important roles in drought tolerance by stimulating plant’s protective genome activities in response to heat and water limitation. TFs are specialized proteins which can bind to specific DNA elements in gene promoters and modulate gene expression in response to various external and internal stimuli. Thus TFs is a crucial part of plant signal transduction pathway mediated by signal receptors, phytohormones and other regulatory compounds. The activities of TFs are closely related to their structure, and their binding specificity is determined by the homo-/hetero-dimerization of TFs. The expression of downstream genes may produce a subset of TFs or regulate other functional proteins involved in physiological drought adaptation. Thus, the hierarchic regulations of TF activities, downstream gene expression and protein–protein interaction comprise a complex regulatory network, which participates in drought response and adaptation in cereal crops. Basic mechanisms of this regulatory network have been described, but more insight is needed to find new tools for enhancing cereals’ adaptation to drought stress

Одержання стабілізованого нанодисперсного заліза на основі органофілізованого монтморилоніту

The synthesis of zero­valent nano­dimension iron on the surface of montmorillonite and organomontmollonite by reducing aqueous solutions of Fe2+ salts by sodium boronhydride was conducted. The results of RFA and IR­spectroscopy indicate formation of a monolayer of SAS (HDTMA) both on the outer surface of the particles of montmorillonite and among structural packages of montmorillonite. In this case, organofilization of the surface contributes to the formation of more dispersed particles of zero­valent iron.The physical and chemical features of the processes of sorption cleaning of contaminated waters from chromium(VI) compounds using the obtained nanodispersed material were explored. It was established that the sorption of ions Cr(VI) by the composite adsorbent based on zero­valent iron and organomontmorillonite amounts to 120 mg/g of Fe, which significantly exceeds sorption for the original montmorillonite, organomontmorillonite, nano­dimension iron and the composite sorbent based on montmorilonite and nano­dimension iron.Based on the study of structural and rheological properties, it was established that with the content of iron in sorbent equal to 0,037÷0,146 %, the suspension remains resistant to aggregation and sedimentation. This, together with their rather high sorption characteristics, makes it appropriate to use aqueous dispersions of organomontmorillonite with the applied layer of the synthesized highly dispersed reactive material when cleaning ground waters from ions of heavy metals (chromium) using the latest environmental technologies based on pumping aqueous dispersions  of nanomaterial  into  contaminated layers of soil.Исследованы физико-химические особенности синтеза наноматериалов на основе монтмориллонита и органомонтморилонита модифицированных нанорозмірним железом. Проведено исследование реологических свойств дисперсий полученных материалов. Показана возможность их использования при очистке подземных вод с применением современных природоохранных технологийДосліджено фізико-хімічні особливості синтезу наноматеріалів на основі монтморилоніту та органомонтморилоніту модифікованих нанорозмірним залізом. Проведено дослідження реологічних властивостей дисперсій отриманих матеріалів. Показано можливість їх використання при очищенні підземних вод із застосуванням сучасних природоохоронних технологі

Sexual dimorphism in the neuro-endocrine regulation of bicycle ergometric test parameters in untrained individuals with dysfunction of the neuro-endocrine-immune complex

Background. Ergometric physical working capacity (PWC) testing has a long tradition in occupational medicine. PWC can be tested, using performance indicators like VO2max or the mechanical power. However, the calculated by bicycle ergometry PWC in reality reflects the reaction of the autonomic nervous system to muscle load, which, in turn, is strong, but still not absolutely complete, correlates with VO2max as a real indicator of cardiorespiratory fitness. The purpose of this study is to clarify the relationship between PWC, calculated based on the result of two-stage bicycle ergometry, and the parameters of neuro-endocrine regulation as well as sexual differences in such relationships. Materials and Methods. The object of observation were 30 women 29-76 (49,4±11,0) years and 30 men 24-69 (47,4±12,0) years without a clinical diagnosis, but with the deviations from the norm in a number of parameters of the neuro-endocrine-immune complex as a manifestation of maladaptation. For estimation of PWC a two-stage bicycle ergometry used. Parameters of EEG, HRV and adaptation hormones levels registered twice with an interval of 4 or 7 days. Results. In men, PWC correlates negatively with plasma levels of cortisol (r=-0,52) and triiodothyronine (r=-0,47), but positively with levels of calcitonin (r=0,25) and testosterone (r=0,22). The coefficient of multiple correlation R=0,705. In women, the correlation of the twice lower PWC with cortisol and calcitonin is weaker (r=-0,31 and 0,18, respectively), and is absent with testosterone and triiodothyronine, instead it was found in relation to aldosterone (r=-0,24); R=0,394. The PWC regression model for men includes 6 HRV and 11 EEG parameters (R=0,846), while for women only the mode HRV (r=-0,56) and two EEG parameters (R=0,608). Conclusion. PWC levels in men are generally downregulated by cortisol, triiodothyronine, sympathetic tone, and θ-rhythm generating neurons, but upregulated by testosterone, calcitonin, vagal tone, and related α-rhythm generating neurons. In women, PWC levels are borderline downregulated by cortisol and aldosterone, but significantly upregulated by circulating catecholamines and β-rhythm generating neurons

Obtaining stabilized nanodispersed iron based on organofilized montmorillonite

Досліджено фізико-хімічні особливості синтезу наноматеріалів на основі монтморилоніту та jрганомонтморилоніту модифікованих нанорозмірним залізом. Проведено дослідження реологічних властивостей дисперсій отриманих матеріалів. Показано можливість їх використання при очищенні підземних вод із застосуванням сучасних природоохоронних технологій

Assessment of the rate of glomerular filtration by a non-invasive method

Background. Existing methods for assessing glomerular filtration rate (GF) are invasive. Therefore, we set ourselves the goal of evaluating the rate of glomerular filtration by a non-invasive method. Materials and Methods. The object of observations were 10 men aged 37-69 years without clinical diagnosis tested twice with 7-days interval. The rate of glomerular filtration was calculated according to endogenous creatinine clearance and the Cockcroft & Gault formula. Systolic and diastolic blood pressure was measured three times in a row. The state of the autonomic nervous system was assessed by the HRV method. Results. We confirmed the significant correlation of GF with age and weight. The screening revealed a significant correlation of GF with a number of blood pressure and HRV parameters. If in the classic formula we replace creatinineemia with systolic blood pressure and HRV-markers of sympathetic tone, we get a formula for estimating the GF with a standard error of 10,9 mL/min vs 2,7 mL/min, but without blood sampling. The regression model, which includes HRV and blood pressure parameters, as well as urinary creatinine concentration, allows estimating GF with a standard error of 12 mL/min. Conclusion. The balneotherapy has a significant effect not only on blood creatinine level, but also on HRV and blood pressure parameters, and even more pronounced. Therefore, the estimation of GF based only on the Cockcroft & Gault formula is at least not much more accurate than the one proposed by us, besides, our method is completely non-invasive

Determination of Safety Indicators in the Developed Muffins with Non-traditional Raw Materials

The aim of research is studying the effect of unconventional raw materials (buckwheat, oatmeal, rye, corn, milk whey, skimmed milk powder, propolis, flower pollen, bean powder, sesame oil, pumpkin seed oil, walnut oil, walnut kernels, candied fruit mixes (apple-cherry fruits of bilberries, natural honey with floral pollen, natural honey with propolis) for the quality and safety of new muffins. The article analyzes the results of the study of the quality and safety indicators of muffins of improved composition, manufactured using non-traditional raw materials. For the preparation of new types of muffins, part of the wheat flour was replaced with buckwheat, corn, oat, milk whey, skimmed milk powder and additives of vegetable origin. Margarine has been replaced by vegetable oils. It has been established that flour confectionery products have high organoleptic properties. From the physico-chemical parameters, the mass fraction of total sugar was determined, which ranged from 28.17 % to 33.07 %, the mass fraction of fat – 16.2–20.66 %, the mass fraction of moisture – 18.0–22.0, mass fraction of ash – 0.03–1.3 %, alkalinity – 0.03–1.8°, the content of toxic elements (copper, zinc, lead, cadmium, arsenic, mercury) and microbiological indicators were also determined. Based on the data obtained as a research result, the expediency of using non-traditional raw materials to expand the range of flour confectionery products, in particular muffins is substantiated

Assessment of the sewage flotation process use

Import 05/08/2014Bakalářská práce je zaměřena do oblasti čistění odpadních vod. Je zde řešena možnost využití flotace k odstraňování sraženiny fosforu vznikající při terciárním čištění odpadní vody. Úvodní část je teoretická a popisuje čištění odpadních vod, flotaci jako separační technologie a srážení fosforu. Druhá část je praktická, která popisuje experimentální zařízení.The thesis is focused on the area of wastewater treatment. There addressed the possibility of using flotation to remove the precipitate formed during the phosphorus tertiary wastewater treatment. The first part is theoretical and describes wastewater treatment, flotation separation technology as a precipitation of phosphorus. The second part is practical, which describes the experimental facility.Prezenční546 - Institut environmentálního inženýrstvívelmi dobř

DETERMINATION OF SAFETY INDICATORS IN THE DEVELOPED MUFFINS WITH NON-TRADITIONAL RAW MATERIALS

The aim of research is studying the effect of unconventional raw materials (buckwheat, oatmeal, rye, corn, milk whey, skimmed milk powder, propolis, flower pollen, bean powder, sesame oil, pumpkin seed oil, walnut oil, walnut kernels, candied fruit mixes (apple-cherry fruits of bilberries, natural honey with floral pollen, natural honey with propolis) for the quality and safety of new muffins. The article analyzes the results of the study of the quality and safety indicators of muffins of improved composition, manufactured using non-traditional raw materials. For the preparation of new types of muffins, part of the wheat flour was replaced with buckwheat, corn, oat, milk whey, skimmed milk powder and additives of vegetable origin. Margarine has been replaced by vegetable oils. It has been established that flour confectionery products have high organoleptic properties. From the physico-chemical parameters, the mass fraction of total sugar was determined, which ranged from 28.17 % to 33.07 %, the mass fraction of fat – 16.2–20.66 %, the mass fraction of moisture – 18.0–22.0, mass fraction of ash – 0.03–1.3 %, alkalinity – 0.03–1.8°, the content of toxic elements (copper, zinc, lead, cadmium, arsenic, mercury) and microbiological indicators were also determined. Based on the data obtained as a research result, the expediency of using non-traditional raw materials to expand the range of flour confectionery products, in particular muffins is substantiated

Ukrainian Iconography of the Late XX–Early XXI Century: Trends and Leading Schools

The article deals with the development of modern Ukrainian iconography, the formation of the main centers and regional schools, traces artistic and stylistic features and trends. The article analyzes the activities of the Kyiv and Lviv Art Schools in the field of sacred art. The main features of the development of modern iconography are identified, the creativity of leading specialists and iconographers, genres and technological specifics of iconography are studied. The scientific novelty of this research consists in a comprehensive study of modern Ukrainian iconography, taking into account artistic and stylistic, regional, technological, and canonical aspects. Features of formation of art schools and associations of sacred art in Ukraine are traced. The aim of the study is to determine the main trends in the development of modern Ukrainian iconography, to identify the influence of leading schools in the field of sacred art. The methodological basis is a comprehensive approach to the analysis of cultural and artistic phenomena in the chronological and theoretical aspects of the development of modern sacred art in Ukraine