Measurement of the cross-section and forward-backward charge asymmetry for the b and c-quark in e+e- annihilation with inclusive muons at sqrt(s) = 58 GeV

We have studied inclusive muon events using all the data collected by the TOPAZ detector at sqrt(s)=58 GeV with an integrated luminosity of 273pb-1. From 1328 inclusive muon events, we measured the ratio R_qq of the cross section for qq-bar production to the total hadronic cross section and forward-backward asymmetry A^q_FB for b and c quarks. The obtained results are R_bb = 0.13+-0.02(stat)+-0.01(syst), R_cc = 0.36+-0.05(stat)+-0.05(syst), A^b_FB = -0.20+-0.16(stat)+-0.01(syst) and A^c_FB = -0.17+-0.14(stat)+-0.02(syst), in fair agreement with a prediction of the standard model.Comment: To be published in EPJ C. 24 pages, 12 figure

A Measurement of the D∗±D^{*\pm} Cross Section in Two-Photon Processes

We have measured the inclusive $D^{*\pm}$ production cross section in a two-photon collision at the TRISTAN $e^+e^-$ collider. The mean $\sqrt{s}$ of the collider was 57.16 GeV and the integrated luminosity was 150 $pb^{-1}$. The differential cross section ($d\sigma(D^{*\pm})/dP_T$) was obtained in the $P_T$ range between 1.6 and 6.6 GeV and compared with theoretical predictions, such as those involving direct and resolved photon processes.Comment: 8 pages, Latex format (article), figures corrected, published in Phys. Rev. D 50 (1994) 187

K0(K0ˉ)K^0(\bar{K^0}) Production in Two-Photon Processes at TRISTAN

We have carried out an inclusive measurement of $K^0(\bar{K^0})$ production in two-photon processes at TRISTAN. The mean $\sqrt{s}$ was 58 GeV and the integrated luminosity was 199 pb$^{-1}$. High-statistics $K_s$ samples were obtained under such conditions as no-, anti-electron, and remnant-jet tags. The remnant-jet tag, in particular, allowed us, for the first time, to measure the cross sections separately for the resolved-photon and direct processes.Comment: 20 pages, Latex format, 4 figures and KEK-mark included. Table 1 revised. To be published in Phys. Lett.

Measurement of inclusive electron cross section in γγ\gamma \gamma collisions at TRISTAN

We have studied open charm production in $\gamma \gamma$ collisions with the TOPAZ detector at the TRISTAN $e^{+}e^{-}$ collider. In this study, charm quarks were identified by electrons (and positrons) from semi-leptonic decays of charmed hadrons. The data corresponded to an integrated luminosity of 95.3 pb$^{-1}$ at a center-of-mass energy of 58 GeV. The results are presented as the cross sections of inclusive electron production in $\gamma \gamma$ collisions with an anti-tag condition, as well as the subprocess cross sections, which correspond to resolved-photon processes. The latter were measured by using a sub-sample with remnant jets. A comparison with various theoretical predictions based on direct and resolved-photon processes showed that our data prefer that with relatively large gluon contents in a photon at small $x (x \le 0.1)$, with the next-to-leading order correction, and with a charm-quark mass of 1.3 GeV.Comment: 26 pages, Latex format (article), 5 figures included, to be published in Phys. Lett.

Measurement of the forward-backward asymmetries for charm- and bottom-quark pair productions at <s><\sqrt{s}>=58GeV with electron tagging

We have measured, with electron tagging, the forward-backward asymmetries of charm- and bottom-quark pair productions at $$=58.01GeV, based on 23,783 hadronic events selected from a data sample of 197pb$^{-1}$ taken with the TOPAZ detector at TRISTAN. The measured forward-backward asymmetries are $A_{FB}^c = -0.49 \pm 0.20(stat.) \pm 0.08 (sys.)$ and $A_{FB}^b = -0.64 \pm 0.35(stat.) \pm 0.13 (sys.)$, which are consistent with the standard model predictions.Comment: 19 pages, Latex format (article), 5 figures included. to be published in Phys. Lett.

Identical NR5A1 Missense Mutations in Two Unrelated 46,XX Individuals with Testicular Tissues

The role of monogenic mutations in the development of 46,XX testicular/ovotesticular disorders of sex development (DSD) remains speculative. Although mutations in NR5A1 are known to cause 46,XY gonadal dysgenesis and 46,XX ovarian insufficiency, such mutations have not been implicated in testicular development of 46,XX gonads. Here, we identified identical NR5A1 mutations in two unrelated Japanese patients with 46,XX testicular/ovotesticular DSD. The p.Arg92Trp mutation was absent from the clinically normal mothers and from 200 unaffected Japanese individuals. In silico analyses scored p.Arg92Trp as probably pathogenic. In vitro assays demonstrated that compared with wild‐type NR5A1, the mutant protein was less sensitive to NR0B1‐induced suppression on the SOX9 enhancer element. Other sequence variants found in the patients were unlikely to be associated with the phenotype. The results raise the possibility that specific mutations in NR5A1 underlie testicular development in genetic females

Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mechanism of establishment

Differential methylation between the two alleles of a gene has been observed in imprinted regions, where the methylation of one allele occurs on a parent-of-origin basis, the inactive X-chromosome in females, and at those loci whose methylation is driven by genetic variants. We have extensively characterized imprinted methylation in a substantial range of normal human tissues, reciprocal genome-wide uniparental disomies, and hydatidiform moles, using a combination of whole-genome bisulfite sequencing and high-density methylation microarrays. This approach allowed us to define methylation profiles at known imprinted domains at base-pair resolution, as well as to identify 21 novel loci harboring parent-of-origin methylation, 15 of which are restricted to the placenta. We observe that the extent of imprinted differentially methylated regions (DMRs) is extremely similar between tissues, with the exception of the placenta. This extra-embryonic tissue often adopts a different methylation profile compared to somatic tissues. Further, we profiled all imprinted DMRs in sperm and embryonic stem cells derived from parthenogenetically activated oocytes, individual blastomeres, and blastocysts, in order to identify primary DMRs and reveal the extent of reprogramming during preimplantation development. Intriguingly, we find that in contrast to ubiquitous imprints, the majority of placenta-specific imprinted DMRs are unmethylated in sperm and all human embryonic stem cells. Therefore, placental-specific imprinting provides evidence for an inheritable epigenetic state that is independent of DNA methylation and the existence of a novel imprinting mechanism at these loci

The Analysis of Receptor-binding Cancer Antigen Expressed on SiSo Cells (RCAS1) immunoreactivity within the microenvironment of the ovarian cancer lesion relative to the applied therapeutic strategy

RCAS1 is involved in generating the suppressive profile of the tumor microenvironment that helps cancer cells evade immune surveillance. The status of the cells surrounding the cancer nest may affect both the progression of the cancer and the development of metastases. In cases of ovarian cancer, a large number of patients do not respond to the applied therapy. The patient’s response to the applied therapy is directly linked to the status of the tumor microenvironment and the intensity of its suppressive profile. We analyzed the immunoreactivity of RCAS1 on the cells present in the ovarian cancer microenvironment in patients with the disease; these cells included macrophages and carcinoma-associated fibroblasts. Later we analyzed the immunoreactivity levels within these cells, taking into consideration the clinical stage of the cancer and the therapeutic strategy applied, such as the number of chemotherapy regiments, primary cytoreductive surgery, or the presence of advanced ascites. In the patients who did not respond to the therapy we observed significantly higher immunoreactivity levels of RCAS1 within the cancer nest than in those patients who did respond; moreover, in the non-responsive patients we found RCAS1 within both macrophages and carcinoma-associated fibroblasts. RCAS1 staining may provide information about the intensity of the immuno-suppressive microenvironment profile found in cases of ovarian cancer and its intensity may directly relate to the clinical outcome of the disease

Quasi-free photoproduction of η-mesons off 3He nuclei

Quasi-free photoproduction of η-mesons has been measured off nucleons bound in 3He nuclei for incident photon energies from the threshold region up to 1.4 GeV. The experiment was performed at the tagged photon facility of the Mainz MAMI accelerator with an almost 4π covering electromagnetic calorimeter, combining the TAPS and Crystal Ball detectors. The η-mesons were detected in coincidence with the recoil nucleons. This allowed a comparison of the production cross section off quasi-free protons and quasi-free neutrons and a full kinematic reconstruction of the final state, eliminating effects from nuclear Fermi motion. In the S11(1535) resonance peak, the data agree with the neutron/proton cross section ratio extracted from measurements with deuteron targets. More importantly, the prominent structure observed in photoproduction off quasi-free neutrons bound in the deuteron is also clearly observed. Its parameters (width, strength) are consistent with the expectations from the deuteron results. On an absolute scale the cross sections for both quasi-free protons and neutrons are suppressed with respect to the deuteron target pointing to significant nuclear final-state interaction effects

Photoproduction of mesons off nuclei

Recent results for the photoproduction of mesons off nuclei are reviewed. These experiments have been performed for two major lines of research related to the properties of the strong interaction. The investigation of nucleon resonances requires light nuclei as targets for the extraction of the isospin composition of the electromagnetic excitations. This is done with quasi-free meson photoproduction off the bound neutron and supplemented with the measurement of coherent photoproduction reactions, serving as spin and/or isospin filters. Furthermore, photoproduction from light and heavy nuclei is a very efficient tool for the study of the interactions of mesons with nuclear matter and the in-medium properties of hadrons. Experiments are currently rapidly developing due to the combination of high quality tagged (and polarized) photon beams with state-of-the-art 4pi detectors and polarized targets