Another win for endothelial progenitor cells: Endothelial progenitor cell-derived conditioned medium promotes proliferation and exerts neuroprotection in cultured neuronal progenitor cells.

Progress in stem cell research demonstrates stem cells' potential for treating neurodegenerative diseases. Stem cells have proliferative/differentiative properties and produce a variety of paracrine factors that can potentially be used to regenerate nervous tissue. Previous studies have shown the positive regenerative effects of endothelial progenitor cells (EPCs), and thus, they may be used as a tool for regeneration. A study by Di Santo et al. explored whether EPC-derived conditioned medium (EPC-CM) promotes the survival of cultured striatal progenitor cells and attempted to find the paracrine factors and signaling pathways involved with EPC-CM's effects. The neuronal progenitor cells that were cultured with EPC-CM had much higher densities of GABA-immunoreactive (GABA-ir) neurons. It was shown that phosphatidylinositol-3-kinase/AKT and mitogen-activated protein kinase/ERK signaling pathways are involved in the proliferation of GABAergic neurons, as inhibition of these pathways decreased GABAergic densities. In addition, the results suggest that paracrine factors from EPC, both proteinaceous and lipidic, significantly elevated the viability and/or differentiation in the cultures. Importantly, it was found that EPC-CM provided neuroprotection against toxins from 3-nitropropionic acid. In sum, EPC-CM engendered proliferation and regeneration of the cultured striatal cells through paracrine factors and imparted neuroprotection. Furthermore, the effects of EPC-CM may generate a cell-free therapeutic strategy to address neurodegeneration

Successful transition to elementary school and the implementation of facilitative practices specified in the Reggio-Emilia philosophy

Systematic, mandated facilitation of school transitions is an important but understudied aspect of the Reggio-Emilia approach to early childhood education admired internationally as best practice. We studied the links between Northern Italian transition practices and academic achievement, school liking, cooperativeness, and problem behaviors. We followed 288 students across a transition from preschool to elementary school. Schools varied in their implementation of transition practices. High implementation of Reggio-type transition practices was related to significantly more school liking and significantly fewer problem behaviors after the transition. At follow-up at the end of the post-transition year, high-implementation schools were still characterized by lower levels of problem behavior. These data indicate that the facilitation of school transitions in the Reggio-Emilia tradition is associated with successful post-transition adjustment

Comparison between off-resonance and electron Bernstein waves heating regime in a microwave discharge ion sourcea)

A microwave discharge ion source (MDIS) operating at the Laboratori Nazionali del Sud of INFN, Catania has been used to compare the traditional electron cyclotron resonance (ECR) heating with an innovative mechanisms of plasma ignition based on the electrostatic Bernstein waves (EBW). EBW are obtained via the inner plasma electromagnetic-to-electrostatic wave conversion and they are absorbed by the plasma at cyclotron resonance harmonics. The heating of plasma by means of EBW at particular frequencies enabled us to reach densities much larger than the cutoff ones. Evidences of EBW generation and absorption together with X-ray emissions due to high energy electrons will be shown. A characterization of the discharge heating process in MDISs as a generalization of the ECR heating mechanism by means of ray tracing will be shown in order to highlight the fundamental physical differences between ECR and EBW heating

Caracterización angular automatizada de sensores solares fotovoltaicos

Este trabajo tiene como objetivo caracterizar la respuesta angular de distintos dispositivos fotovoltaicos al variar el ángulo de la radiación solar incidente. La caracterización se llevó a cabo con un nuevo equipo automatizado de respuesta angular desarrollado en el Departamento de Energía Solar (DES) de la Comisión Nacional de Energía Atómica (CNEA). Se presenta la caracterización de sensores solares y radiómetros fotovoltaicos desarrollados en el DES y dispositivos comerciales similares. Se analizó la repetitividad de la respuesta entre varios sensores de radiación fotosintéticamente activa (PAR) de una misma serie de fabricación nacional y se comparó la respuesta de los diferentes sensores elaborados en el DES con la de los sensores comerciales de igual principio de funcionamiento. En el caso de los sensores PAR nacionales, el análisis de las mediciones llevó a una modificación del difusor del sensor; la mejora en la respuesta se pudo comprobar ensayando nuevamente los sensores.The main objective of this paper is to characterize the angular response of different photovoltaic devices by varying the angle of incident solar radiation. In order to achieve this, new automated angular response equipment made in the Department of Solar Energy (DES) of the National Atomic Energy Commission (CNEA) is being used. The characterization of solar sensors and photovoltaic radiometers developed in DES and commercial devices with similar characteristics is presented. The repetitiveness of the response between several photosynthetically active radiation sensors (PAR) of the same series of national manufacture was analyzed and the response of the different sensors developed in the DES with the response of commercial sensors of the same operating principle was compared. In the case of the national PAR sensors, the analysis of the results obtained led to a modification of the diffuser; the improvement in the response was checked by retesting the sensors.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

On the management of nature-based solutions in open-air laboratories: new insights and future perspectives

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste

Incapsulation of azo- and xanthene-tattoo pigments in lipid microparticles colorant photo-stabilization and retention by the particle matrix in excised porcine skin

No abstract availabl

Macrotrachela quadricornifera featured in a space experiment

Macrotrachela quadricornifera, a bdelloid rotifer, is the animal model of an experiment scheduled on the International Space Station (ISS). The focus of the experiment is the role of the cytoskeleton during oogenesis and early development. Bdelloids will fly desiccated, be rehydrated on the ISS and cultivated for five generations. We present the outline of the ISS experiment, the expectations and the state-of-the-art of ground-based research run to date on the major topics of the planned experiment: anhydrobiosis and embryogenesis. Anhydrobiosis focuses on two major aspects, storage conditions that enhance recovery rate and comparison of the resistance between dormant and active rotifers to UV radiation and other environmental injuries. Embryogenesis has been approached at the morphological level under ground conditions: developing embryos have been studied by light and confocal microscopes

The Toxicity of Polyester Fibers in Xenopuslaevis

Microplastics are practically ubiquitous and pose a serious survival challenge for many species. Most of the exposure experiments for determining the toxicological effects of microplastics were performed with a microplastic varying little in shape and size (often purchased microplastic beads), but few studies deal with non-homogeneous samples. We analyzed the effect on Xenopuslaevis larva on the early development of polyester fibers, PEFs, taken from a dryer machine in which 100% polyester fabrics were dried after washing. Three concentrations were tested. The results showed that the gastrointestinal tract, GIT, was the most affected system by PEFs which modified the normal shape of the intestine with an EC50 96 h value of 6.3 µg mL−1. Fibers were observed to press against the digestive epithelium, deforming the normal architecture of the gut, sometimes pushing deep into the epithelium until piercing it. Physical GIT occlusion was observed in a concentration-dependent manner. However, no other damages were registered. No mortality was observed, but PEF-exposed larvae showed a significant reduction in their mobility. The results of the present paper suggest that environmental samples with their heterogeneity may have adverse effects on X. laevis development